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| 1 | The alteration in the architecture of a T-DNA insertion rice mutant osmtd1 is caused by up-regulation of Micro RNA156f显示文摘Plant architecture is an important factor for crop production. Some members of micro RNA156(mi R156) and their target genes SQUAMOSA Promoter-Binding Protein-Like(SPL) were identi fi ed to play essential roles in the establishment of plant architecture. However, the roles and regulation of mi R156 is not well understood yet. Here, we identi fi ed a T-DNA insertion mutant Osmtd1(Oryza sativa multi-tillering and dwarf mutant). Osmtd1 produced more tillers and displayed short stature phenotype. We determined that the dramatic morphological changes were caused by a single T-DNA insertion in Osmtd1. Further analysis revealed that the T-DNA insertion was located in the gene Os08g34258 encoding a putative inhibitor I family protein. Os08g34258 was knocked out and Osmi R156 f was signi fi cantly upregulated in Osmtd1. Overexpression of Os08g34258 in Osmtd1 complemented the defects of the mutant architecture, while overexpression of Osmi R156 f in wild-type rice phenocopied Osmtd1. We showed that the expression of Os SPL3, Os SPL12,and Os SPL14 were signi fi cantly downregulated in Osmtd1 or Osmi R156 f overexpressed lines, indicating that Os SPL3,Os SPL12, and Os SPL14 were possibly direct target genes of Osmi R156 f. Our results suggested that Osmi R156 f controlledplant architecture by mediating plant stature and tiller outgrowth and may be regulated by an unknown protease inhibitor I family protein. | Qing Liu Gezhi Shen Keqin Peng Zhigang Huang Jianhua Tong Mohammed Humayun Kabir Jianhui Wang Jingzhe Zhang Genji Qin Langtao Xiao | 2015 | Journal of Integrative Plant Biology2015，57，10： | 5 |
| 2 | Retinoschisis at Macular Area in Highly Myopic Eye by Optic Coherence Tomography显示文摘Purpose:To improve our understanding of macular schisis in highly myopic eyes. Methods:From 2002.6-2003.12, the highly myopic eyes(>6D) which has macular schisis found by OCT at our hospital were included in the study. Each eye then received further examinations including visual acuity,refractive error and ultra-sonography. Then the results were analyzed to find the characteristic of the entity. Results:During that period, OCT found macular schisis in 38 highly myopic eyes from 36 patients. Their vision was low, and their average axial length and refractive error were 29.6mm and -10.5D respectively. The appearance and the area of the schisis are different among the eyes. But the schisis can be largely divided into two types: inner and outer schisis. They can exit independently or concurrently in the same eye. And the schisis was often accompanied by other macular findings, like retinal detachment, macular hole. Conclusion:Macular schisis in highly myopic eyes is not uncommon in the clinic. OCT is useful in its diagnosis. | Chunhui Jiang Wenji Wang Gezhi Xu Ling Wang | 2006 | 眼科学报2006，22，3： | 2 |
| 3 | Safety and Efficacy of Conbercept in Neovascular Age-Related Macular Degeneration Results from a 12-month Randomized Phase II Study: AURORA Study显示文摘 | Xiaoxin Li Gezhi Xu Yusheng Wang Xun Xu Xiaoling Liu Shibo Tang Feng Zhang Junjun Zhang Luosheng Tang Quan Wu Delun Luo Xiao Ke | 2014 | Ophthalmology2014，，： | 2 |
| 4 | Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit显示文摘 | ZhenYing Shi Jiang Wang XinShan Wan GeZhi Shen XinQi Wang JingLiu Zhang | 2007 | Planta2007，，1： | 1 |
| 5 | Complexity in Biological signaling systems 显示文摘 | Weng Gezhi Bhalla Upinder S Iyengr Ravi | 1999 | Science1999，284，： | 1 |
| 6 | G protein b_1λ_2 subunits purification and their interaction with adenylyl cyclase显示文摘A preliminary study on the interaction of G protein (guanine triphosphate binding pro- tein) b1g2 subunits and their coupled components in cell signal transduction was conducted in vitro. The insect cell lines, Sf9 (Spodoptera frugiperda) and H5 (Trichoplusia ni ) were used to express the recombinant protein Gb1g2. The cell membrane containing Gb1g2 was isolated through affinity chromatography column with Ni-NTA agarose by FPLC method, and the highly purified protein was obtained. The adenylyl cyclase 2 (AC2) activity assay showed that the purified Gb1g2 could signifi-cantly stimulate AC2 activity. The interaction of b1g2 subunits of G protein with the cytoplasmic tail of various mammalian adenylyl cyclases was monitored by BIAcore technology using NTA sensor chip, which relies on the phenomenon of surface plasmon resonance (SPR). The experiments showed the direct binding of Gb1g2 to the cytoplasmic tail C2 domain of AC2. The specific binding domain of AC2 with Gb1g2 was the same as AC2 activity domain which was stimulated by Gb1g2. | 陈巨莲 倪汉祥 孙京瑞 WENG Gezhi | 2003 | Science China(Life Sciences)2003，46，2： | 1 |
| 7 | Complexity in biological signaling systems 显示文摘 | Bhalla U S Iyengar R | 1999 | Science1999，284，： | 1 |
| 8 | The amylose content in rice endosperm is related to the post-transcriptional regulation of the Waxy gene显示文摘 | Wang Zongyang Zheng Feiqin Shen Gezhi | 1995 | The Plant Journal1995，7，4： | 1 |
| 9 | Transformation of dissociation transposon in japonica rice Zhonghua 11 mediated by Agrobacterium显示文摘Dissociation(Ds)transposon is one of thetransposable elements in corn.The trans-posons can be transferred into other plantswhere the transposons were not found.Oncethe transposon was inserted into target gene ofthese plants,it could be used as a marker todistinguish and isolate the gene.The object ofthis study is to transfer Ds transposon to riceby Agrobacterium-mediated transformation. The calli of immature embryos。 | ZHANG Jianjun YIN Liqin WANG Xinqi FAN Kunhua CHEN Quanqing SHEN Gezhi,Crop Res Inst,Shanghai Acad of Agri Sci,Shanghai 201107,China | 1999 | Chinese Rice Research Newsletter1999，7，2： | 1 |
| 10 | Photodynamic Therapy for Symptomatic Circumscribed Macular Choroidal Hemangioma in Chinese Patients显示文摘 | Yongjin Zhang Wei Liu Yanwen Fang Jiang Qian Gezhi Xu Wenji Wang Lei Li Ying Shen Qiaoyun Gao | 2010 | American Journal of Ophthalmology2010，，5： | 1 |
| 11 | Degradation strategies of pesticide residue:From chemicals tosynthetic biology显示文摘The past 50 years have witnessed a massive expansion in the demand and application of pesticides.However,pesticides are difficult to be completely degraded without intervention hence the pesticide residue could pose a persistent threat to non-target organisms in many aspects.To aim at the problem of the abuse of pesticide products and excessive pesticide residues in the environment,chemical and biological degradation methods are widely developed but are scaled and insufficient to solve such a pollution.In recent years,bio-degradative tools instructed by synthetic biological principles have been further studied and have paved a way for pesticide degradation.Combining the customized design strategy and standardized assembly mode,the engineering bacteria for multi-dimensional degradation has become an effective tool for pesticide residue degradation.This review introduces the mechanisms and hazards of different pesticides,summarizes the methods applied in the degradation of pesticide residues,and discusses the advantages,applications,and prospects of synthetic biology in degrading pesticide residues. | Bi Ruomeng Ou Meihao Zhou Siru Geng Shichen Zheng Yixian Chen Junhong Mo Ruijie Li Yuan Xiao Gezhi Chen Xingyu Zhai Shiyi Zhang Aihui Fang Baishan | 2023 | Synthetic and Systems Biotechnology2023，8，2： | 0 |
| 12 | Framework nucleic acid-based confined enzyme cascade for efficient synergistic cancer therapy in vivo显示文摘Artificial enzyme cascade systems with confinement effect are highly important in synthetic biology and biomedicine.Herein,a framework nucleic acid-based confined enzyme cascade(FNA-CEC)for synergistic cancer therapy in vivo was developed.The FNA-CEC consisted of glucose oxidase and horseradish peroxidase precisely assembled on an addressable DNA tetrahedron scaffold within few nanometers.Glucose oxidase(GOx)can trigger efficient glucose depletion for tumor starvation therapy,and increase the local concentration of H_(2)O_(2) in situ for enhanced downstream horseradish peroxidase(HRP)-activated prodrug therapy.Due to the spatial-confinement on DNA tetrahedron scaffold,the efficiency of intermediate metabolites transportation between the enzyme cascades was improved.Moreover,FNA-CEC was applied for efficient synergistic cancer therapy in vitro and in vivo.As a simple and efficient approach,the FNA-CEC is expected to expand the toolbox of technologies in synthetic biology and biomedicine. | Gezhi Kong Meng Zhang Mengyi Xiong Mei Chen Chan Yang Yan Zhao Yue Yang Zilong Zhao Zhi-Ling Song Liang Gong Guoliang Ke Xiao-Bing Zhang Weihong Tan | 2021 | Science China Chemistry2021，64，4： | 0 |
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