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| 1 | Human ACE2-Functionalized Gold“Virus-Trap”Nanostructures for Accurate Capture of SARS-CoV-2 and Single-Virus SERS Detection显示文摘The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus.Here,we present a Human Angiotensin-converting-enzyme 2(ACE2)-functionalized gold“virus traps”nanostructure as an extremely sensitive SERS biosensor,to selectively capture and rapidly detect S-protein expressed coronavirus,such as the current SARS-CoV-2 in the contaminated water,down to the single-virus level.Such a SERS sensor features extraordinary 106-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as“virus-traps”composed of oblique gold nanoneedles,and 109-fold enhancement of Raman signals originating from multi-component SERS effects.Furthermore,the identification standard of virus signals is established by machine-learning and identification techniques,resulting in an especially low detection limit of 80 copies mL^(−1) for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min,which is of great significance for achieving real-time monitoring and early warning of coronavirus.Moreover,here-developed method can be used to establish the identification standard for future unknown coronavirus,and immediately enable extremely sensitive and rapid detection of novel virus. | Yong Yang Yusi Peng Chenglong Lin Li Long Jingying Hu Jun He Hui Zeng Zhengren Huang Zhi-Yuan Li Masaki Tanemura Jianlin Shi John R.Lombardi Xiaoying Luo | 2021 | Nano-Micro Letters2021，13，7： | 6 |
| 2 | Continuous desulfurization and bacterial community structure of an integrated bioreactor developed to treat SO_2 from a gas stream显示文摘Sulfide dioxide(SO2) is often released during the combustion processes of fossil fuels. An integrated bioreactor with two sections, namely, a suspended zone(SZ) and immobilized zone(IZ), was applied to treat SO2 for 6 months. Sampling ports were set in both sections to investigate the performance and microbial characteristics of the integrated bioreactor. SO2 was effectively removed by the synergistic effect of the SZ and IZ, and more than 85%removal efficiency was achieved at steady state. The average elimination capacity of SO2 in the bioreactor was 2.80 g/(m3·hr) for the SZ and 1.50 g/(m3· hr) for the IZ. Most SO2 was eliminated in the SZ. The liquid level of the SZ and the water content ratio of the packing material in the IZ affected SO2 removal efficiency. The SZ served a key function not only in SO2 elimination, but also in moisture maintenance for the IZ. The desired water content in IZ could be feasibly maintained without any additional pre-humidification facilities. Clone libraries of 16 S r DNA directly amplified from the DNA of each sample were constructed and sequenced to analyze the community composition and diversity in the individual zones.The desulfurization bacteria dominated both zones. Paenibacillus sp. was present in both zones, whereas Ralstonia sp. existed only in the SZ. The transfer of SO2 to the SZ involved dissolution in the nutrient solution and biodegradation by the sulfur-oxidizing bacteria.This work presents a potential biological treatment method for waste gases containing hydrophilic compounds. | Jian Lin Lin Li Wenjie Ding Jingying Zhang Junxin Liu | 2015 | Journal of Environmental Sciences2015，27，11： | 6 |
| 3 | A full-scale integrated-bioreactor with two zones treating odours from sludge thickening tank and dewatering house: performance and microbial characteristics显示文摘一一个推迟的地区和一个使不能调动的地区的照原尺寸的综合生物反应器的在于被采用对待从废水处理植物射出的 ordours。H 2 S 和 NH 3 分别地是 1.6-38.6 mgm -3 和 0.1-6.7 mgm -3, ,当不变的插头集中为 H 2-3 时 > 为 NH 3 的 S 和 0-0.5 mgm -3 。两 H 2 S 和 NH 3 有效地被消除由综合生物反应器。H 2 S 和 NH 3 在二个地区之间不同。与 H 2 S 和 NH 3 被孤立。在生物反应器的微生物的特征和分布在单个地区取决于底层和微环境的条件的入口集中。产品分析显示了 H 2 S 在使不能调动的地区被氧化进硫酸盐,但是在推迟的地区被溶解进液体阶段。大量 NH 3 被硝代在推迟的地区变换成硝酸盐和亚硝酸根,而仅仅 NH 3 的小数量主要被吸收或化学中立化在使不能调动的地区转移到水的阶段。不同微生物引起的人口统治了单个地区,并且主要 biodegradation 产品因此变化了。 | Jianwei Liu Kaixiong Yang Lin Li Jingying Zhang | 2017 | Frontiers of Environmental Science & Engineering2017，11，4： | 3 |
| 4 | PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness显示文摘PHYTOCHROME-INTERACTING FACTORS(PIFs)are a group of basic helix-loop-helix transcription factors that can physically interact with photoreceptors,including phytochromes and cryptochromes.It was previously demonstrated that PIFs accumulated in darkness and repressed seedling photomorphogenesis,and that PIFs linked different photosensory and hormonal pathways to control plant growth and development.In this study,we show that PIFs positively regulate the ABA signaling pathway during the seedling stage specifically in darkness.We found that PIFs positively regulate ABI5 transcript and protein levels in darkness in response to exogenous ABA treatment by binding directly to the G-box motifs in the ABI5 promoter.Consistently,PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness,and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark.Moreover,we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9,and that this interaction is not regulated by ABA.Further analyses showed that PYL8 and PYL9 promote PIF4 protein accumulation in the dark and enhance PIF4 binding to the ABI5 promoter,but negatively regulate PIF4-mediated ABI5 activation.Taken together,our data demonstrate that PIFs interact with ABA receptors to orchestrate ABA signaling in darkness by controlling ABI5 expression,providing new insights into the pivotal roles of PIFs as signal integrators in regulating plant growth and development. | Lijuan Qi Shan Liu Cong Li Jingying Fu Yanjun Jing Jinkui Cheng Hong Li Dun Zhang Xiaoji Wang Xiaojing Dong Run Han Bosheng Li Yu Zhang Zhen Li William Terzaghi Chun-Peng Song Rongcheng Lin Zhizhong Gong Jigang Li | 2020 | Molecular Plant2020，13，3： | 3 |
| 5 | Temporal variation of microbial population in a thermophilic biofilter for SO_2 removal显示文摘The performance of a biofilter relies on the activity of microorganisms during the gas contaminant treatment process. In this study, SO_2 was treated using a laboratory-scale biofilter packed with polyurethane foam cubes(PUFC), on which thermophilic desulfurization bacteria were attached. The thermophilic biofilter effectively reduced SO_2 within 10 months of operation time, with a maximum elimination capacity of 48.29 g/m^3/hr.Temporal shifts in the microbial population in the thermophilic biofilter were determined through polymerase chain reaction-denaturing gradient gel electrophoresis and deoxyribonucleic acid(DNA) sequence analysis. The substrate species and environmental conditions in the biofilter influenced the microbial population. Oxygen distribution in the PUFC was analyzed using a microelectrode. When the water-containing rate in PUFC was over 98%, the oxygen distribution presented aerobic–anoxic–aerobic states along the test route on the PUFC. The appearance of sulfate-reducing bacteria was caused by the anaerobic conditions and sulfate formation after 4 months of operation. | Jingying Zhang Lin Li Junxin Liu | 2016 | Journal of Environmental Sciences2016，28，1： | 2 |
| 6 | Effects of oxygen and water content on microbial distribution in the polyurethane foam cubes of a biofilter for SO_2 removal显示文摘The performance of a biofilter for off-gas treatment relies on the activity of microorganisms and adequate O_2 and H_2O. In present study, a microelectrode was applied to analyze O_2 in polyurethane foam cubes(PUFCs) packed in a biofilter for SO_2 removal. The O_2 distribution varied with the density and water-containing rate(WCR) of PUFCs. The O_2 concentration dropped sharply from 10.2 to 0.8 mg/L from the surface to the center of a PUFC with 97.20%of WCR. The PUFCs with high WCR presented aerobic–anoxic–aerobic areas.Three-dimensional simulated images demonstrated that the structure of PUFCs with high WCR consisted of an aerobic 'shell' and an anoxic 'core', with high-density PUFCs featuring a larger anoxic area than low-density PUFCs. Moreover, the H_2O distribution in the PUFC was uneven and affected the O_2 concentration. Whereas aerobic bacteria were observed in the PUFC surface, facultative anaerobic microorganisms were found at the PUFC core, where the O_2 concentration was relatively low. O_2 and H_2O distributions differed in the PUFCs, and the distribution of microorganisms varied accordingly. | Jingying Zhang Lin Li Junxin Liu Yanjie Wang | 2018 | Journal of Environmental Sciences2018，30，1： | 2 |
| 7 | Simultaneous desulfurization and denitrification by microwave reactor with ammonium bicarbonate and zeolite显示文摘 | Wei Zaishan Lin Zhehang Niu He Jingying | 2009 | Journal of Hazardous Materials2009，162，： | 1 |
| 8 | True-color real-time imaging and spectroscopy of carbon nanotubes on substrates using enhanced Rayleigh scattering显示文摘白光照亮的围单人赛的碳 nanotubes (SWCNT ) 应该由于散布的回声瑞利显得有颜色。然而,底层上的 SWCNT 的真彩成像没被报导,因为 SWCNT 和散布的强壮的底层的极其低的散布紧张。这里,我们证明散布的瑞利能被接口偶极子改进效果极大地提高。底层上的因而丰富多彩的 SWCNT 能被宽地 supercontinuum 激光照明直接在一台光显微镜下面想象,它便于单个 SWCNT 的高产量 chirality 赋值。这条途径,称为的瑞利成像显微镜学,没被限制为 SWCNT,但是对许多 nanomaterials 广泛地适用,它使丰富多彩的 nanoworld 能在光显微镜下面被探索。 | Wenyun Wu Jingying Yue Xiaoyang Lin Dongqi Li Fangqiang Zhu Xue Yin Jun Zhu Jiangtao Wang Jin Zhang Yuan Chen Xinhe Wang Tianyi Li Yujun He Xingcan Dai Peng Liu Yang wei Jiaping Wang Wei Zhang Yidong Huang Li Fan Lina Zhang Qunqing Li Shoushan Fan Kaili Jiang | 2015 | Nano Research2015，8，8： | 1 |
| 9 | Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance显示文摘Exploiting novel endogenous glyphosate-tolerant alleles is highly desirable and has promising potential for weed control in rice breeding. Here,through fusions of different effective cytosine and adenine deaminases with nCas9-NG, we engineered an effective surrogate two-component composite base editing system, STCBE-2, with improved C-to-T and A-to-G base editing efficiency and expanded the editing window. Furthermore,we targeted a rice endogenous OsEPSPS gene for artificial evolution through STCBE-2-mediated near-saturated mutagenesis. After hygromycin and glyphosate selection, we identified a novel OsEPSPS allele with an Asp-213-Asn(D213N)mutation(OsEPSPS-D213N) in the predicted glyphosate-binding domain, which conferred rice plants reliable glyphosate tolerance and had not been reported or applied in rice breeding. Collectively, we developed a novel dual base editor which will be valuable for artificial evolution of important genes in crops. And the novel glyphosate-tolerant rice germplasm generated in this study will benefit weeds management in rice paddy fields. | Chen Zhang Xue Zhong Shaoya Li Lei Yan Jingying Li Yubing He Yong Lin Yangjun Zhang Lanqin Xia | 2023 | Journal of Integrative Plant Biology2023，65，9： | 1 |
| 10 | Correlation between infiltration of FOXP3 + regulatory T cells and expression of B7-H1 in the tumor tissues of gastric cancer显示文摘 | Jingying Hou Zhong Yu Rengyun Xiang Chuqiang Li Lin Wang Shufen Chen Qingyun Li Mei Chen Linyun Wang | 2013 | Experimental and Molecular Pathology2013，，： | 1 |
| 11 | Making a Higher Hit Ratio Cryptanalytic Time-Memory Trade-Off Attack on Passwords显示文摘 | ZOU JingI LIN Dongdai HAO Chunhui LI Zhenqi WANG Wenhao LU Yao | 2013 | Chinese Journal of Electronics2013，22，4： | 1 |
| 12 | Thermophilic biofilter for SO2removal:Performance and microbial characteristics显示文摘 | Zhang Jingying Li Lin Liu Junxin | 2015 | Bioresource Technology2015，180，： | 1 |
| 13 | In vivo volumetric imaging of microcirculation within human skin under psoriatic conditions u- sing optical microangiography 显示文摘 | QIN Jia JIANG Jingying AN Lin | 2011 | Lasers in Surgery and Medi- cine2011，43，2： | 1 |
| 14 | Optimization of Hybrid Crystal with SAPO-5/34 on Hydrothermal Stability for deNOx Reaction by NH3显示文摘In order to deal with the challenge of the hydrothermal deactivation of selective catalytic reduction of NO by NH3(NH3-SCR)catalyst and extend its lifetime,a novel Cu/SAPO-5/34 catalyst was prepared,and it almostmaintains its deNOx performance with a high conversion rate of 90%NO,between 175℃and 400℃after under-going the rigorous treatment at 800℃for 12 h.Thus,Cu/SAPO-5/34 is more recalcitrant to the high-temperature hydrothermal deactivation than Cu/SAPO-34.Besides,the formation of N20 is always below 3×10^-6(3 ppm)during the whole reaction temperature.performing an advanced catalytic selectivity.The effect of high-temperature hvdro-thermal treatment on the morphology,structure and texture property,the acid sites,as well as the active copper spe cies were investigated.These characterizations manifest that the optimized high-temperature hydrothermal stability is associated closely with the good structural stability over Cu/SAPO-5/34-HT.which facilitates to preserve reaction sites,and then showing the better hydrothermal stability than Cu/SAPO-34. | LIN Qingjin LIN Chenlu LIU Jingying LIU Shuang XU Haidi CHEN Yaoqiang DAN Yi | 2020 | Chemical Research in Chinese Universities2020，36，6： | 0 |
| 15 | Benefit analysis of multi-approach biomass energy utilization toward carbon neutrality显示文摘To reduce greenhouse gas(GHG)emissions,biomass has been increasingly developed as a renewable and clean alternative to fossil fuels because of its carbon-neutral characteristics.China has been investigating the rational development and use of bioenergy for developing its clean energy and achieving carbon neutrality.Substituting fossil fuels with multi-source and multi-approach utilized bioenergy and corresponding carbon reduction in China remain largely unexplored.Here,a comprehensive bioenergy accounting model with a multi-dimensional analysis was developed by combining spatial,life cycle,and multi-path analyses.Accordingly,the bioenergy production potential and GHG emission reduction for each distinct type of biomass feedstock through different conversion pathways were estimated.The sum of all available organic waste(21.55 EJ yr^(-1))and energy plants on marginal land(11.77 EJ yr^(-1))in China produced 23.30 EJ of bioenergy and reduced 2,535.32 Mt CO_(2)-eq emissions,accounting for 19.48%and 25.61%of China’s total energy production and carbon emissions in 2020,respectively.When focusing on the carbon emission mitigation potential of substituting bioenergy for conventional counterparts,bioelectricity was the most effective,and its potential was 4.45 and 8.58 times higher than that of gaseous and liquid fuel alternatives,respectively.In this study,life cycle emission reductions were maximized by a mix of bioenergy end uses based on biomass properties,with an optimal 78.56%bioenergy allocation from biodiesel,densified solid biofuel,biohydrogen,and biochar.The main regional bioenergy GHG mitigation focused on the Jiangsu,Sichuan,Guangxi,Henan,and Guangdong provinces,contributing to 31.32%of the total GHG mitigation potential.This study provides valuable guidance on exploiting untapped biomass resources in China to secure carbon neutrality by 2060. | Jiaoyue Wang Jingying Fu Zhitong Zhao Longfei Bing Fengming Xi Feng Wang Jiang Dong Shiyun Wang Gang Lin Yan Yin Qinqin Hu | 2023 | The Innovation2023，4，3： | 0 |
| 16 | Interface dipole enhancement effect and enhanced Rayleigh scattering显示文摘一纳米或亚纳米的光效果包围象围单人赛的碳 nanotubes (SWCNT ) 那样的单个 nanomaterials 的压缩分子的界面的层理论上并且试验性地被学习了。当由光照亮了时,这界面的层作为一个光偶极子格子表现并且贡献在附近的原子,分子,或 nanomaterials 上提高本地地的一块即时近的地,它可以接着导致提高的瑞利散布,散布的拉曼,和荧光。这接口偶极子的理论提高了效果(IDEE ) 预言那在 nanomaterials 和界面的层的飞机之间的更小的距离,或到包围媒介的界面的层的绝缘的常数的更大的比率,将导致一个更大的领域改进因素。这预言被 SWCNT 以及在逐渐地控告的 SWCNT 散布的 situ 瑞利散布的提高的瑞利的几实现进一步试验性地验证。接口偶极子提高了散布的瑞利不仅启用真彩 nanomaterials 的即时成像,而且提供一个有效工具凝视进微妙的界面的现象。 | Wenyun Wu Jingying Yue Dongqi Li Xiaoyang Lin Fangqiang Zhu Xue Yin Jun Zhu Xingcan Dai Peng Liu Yang Wei Jiaping Wang Haitao Yang Lina Zhang Qunqing Li Shoushan Fan Kaili Jiang | 2015 | Nano Research2015，8，1： | 0 |
| 17 | Pollution characteristics of peroxyacetyl nitrate in karst areas in Southwest China显示文摘桂林是世界著名的地处喀斯特地区的旅游胜地,其光化学污染问题日益严重,过氧乙酰硝酸酯(PAN)被认为是光化学污染的可靠指标,也是本研究的重点.本研究于2021年10月首次观测了桂林的PAN的浓度为0.087-2.559ppb,同时探讨了PAN典型高值过程的成因.此次污染主要来源于东北方向污染气团的水平和高空输送,同时,高温,强辐射和低湿度等气象条件也促进了本地PAN的形成.本研究同时估算了桂林市的O3背景浓度为20.347ppb.这项研究为城市的光化学污染控制工作提供了理论基础。 | Songjun Guo Xu Wei Hongjiao Li Wen Qin Yijun Mu Jiongli Huang Chuan Nong Junchao Yang Dabiao Zhang Hua Lin Jingying Mao Zhaoyu Mo | 2024 | Atmospheric and Oceanic Science Letters2024，17，2： | 0 |
| 18 | Molecular characterization,expression patterns,and subcellular localization of a classical and a novel nonclassical MHC class Iαmolecules from Japanese eel Anguilla japonica显示文摘Major histocompatibility complex(MHC)molecules play a critical role in the immune response of vertebrate animals by presenting foreign antigens to T lymphocytes.In this study,we first cloned and identified a classical and a novel nonclassical MHC Iαmolecules from Japanese eel(Anguilla japonica),named as AjMHC I-UBA and AjMHC I-L,respectively.The full-length cDNA of AjMHC I-UBA contains an open reading frame(ORF)of 1047 bp encoding a predicted protein of 348 amino acids,while AjMHC I-L 1089 bp encodes 362 amino acids.The multiple alignment of the amino acid sequence showed that AjMHC I-UBA and AjMHC I-L consist of an N-terminal MHC I superfamily domain withinα1 andα2 helices,an IgC-MHC Iα3 domain,and a transmembrane region close to the C-terminal,which are similar to other fish and mammal species.Molecular polymorphism analysis showed that eight different major alleles of AjMHC I-UBA,named as AjMHC I-UBA*0101~1001,were identified from six Japanese eel individuals.Furthermore,a distinguishing signature of the nonclassical L-lineage genes specific motif“HINMTL”,including an N-glycosylation site(NXS/T),was present in theα3 domain of AjMHC I-L sequences.Although the predicted three-dimensional structures of AjMHC I-UBA and AjMHC I-L are similar to that of human MHC Iα,phylogenetic analysis showed that these two protein molecules belong to classical MHC I UBA gene of U-lineage and nonclassical MHC I gene of L-lineage,respectively.Quantitative real-time polymerase chain reaction(qRT-PCR)analysis revealed that the highest expression of AjMHC I-UBA and AjMHC I-L was found in the intestine and the expression level of AjMHC I-UBA in all of the examined tissues was significantly higher than that of AjMHC I-L.The expressions of AjMHC I-UBA and AjMHC I-L in liver,kidney and spleen were significantly induced following injection with the viral mimic poly I:C,LPS,and Aeromonas hydrophila infection.In vitro,the AjMHC I-UBA and AjMHC I-L transcripts of Japanese eel liver cells were significantly enhanced by the treatment of poly I:C or the stimulation of the high concentration of A.hydrophila.Subcellular localization showed that under natural conditions,AjMHC I-UBA and AjMHC I-L were uniformly distributed in the cytoplasm,and aggregated into spots or flakes in the cytoplasm after the stimulation of poly I:C or LPS.These results collectively suggested that AjMHC I-UBA and AjMHC I-L are important components possibly involved in Japanese eel defense against viral and bacterial infection.Taken together,these findings provide valuable insight into the immune function of MHC class I in the immune system of teleost. | Jianjun Feng Xinwei Peng Peng Lin Yilei Wang Ziping Zhang Yuankai Xu Pengyun Chen Jingying Wu | 2023 | Aquaculture and Fisheries2023，8，2： | 0 |
| 19 | Promoting Proton Migration Kinetics by Ni^(2+)Regulating Enables Improved Aqueous Zn-MnO_(2) Batteries显示文摘The energy storage behaviors of MnO_(2) for aqueous Zn-MnO_(2) batteries mainly depend on the Zn^(2+)/H^(+)intercalation but are limited by poor ion/electron migration dynamics and stability.Herein,a strategy is proposed that promoting proton migration kinetics ameliorates H^(+)storage activity by introducing Ni^(2+)intoγ-MnO_(2)(Ni-MnO_(2)).Ni^(2+)can lower the diffusion barrier of H^(+)and selectively induce the ion intercalation,thereby alleviating the electrostatic interaction with the lattice.Moreover,Ni^(2+)enables the adjacent[MnO6]octahedrons to have better electron conductivity.The Ni-MnO_(2) exhibits superior rate performance(nearly four times specific capacity compared with MnO_(2))and ultra-long-cycle stability(100%of capacity retention after 11000 cycles at 3.0 A g^(-1)).The calculation indicates that the Ni-MnO_(2) allows H^(+)migrate rapidly along the one-dimensional tunnel due to reduction of the activation energy caused by Ni^(2+)regulating,thus achieving excellent reaction kinetics.This work brings great potential for the development of high-performance aqueous Zn-MnO_(2) batteries. | Jie Ji Jia Yao Yongchang Xu Houzhao Wan Bao Zhang Lin Lv Jingying Li Nengze Wang Zhaohan Zheng Jun Zhang Guokun Ma Li Tao Hanbin Wang Yi Wang Hao Wang | 2023 | Energy & Environmental Materials2023，6，2： | 0 |
| 20 | Associations between LMO1 gene polymorphisms and central nervous system tumor susceptibility显示文摘Importance:LIM domain only 1(LMO1)gene polymorphisms were previously found to be implicated in the risk of several cancers.No available studies were performed regarding the predisposing effect of LMO1 gene single nucleotide polymorphisms(SNPs)on central nervous system(CNS)tumor risk.Objective:We aimed to determine whether the LMO1 gene SNPs were associated with the risk of CNS tumor by applying a case-control study with 191 cases and 248 controls in China.Methods:The contributions of LMO1 gene SNPs to the risk of CNS tumor was evaluated by multinomial logistic regression.Results:Based on the calculations of odds ratio(OR)and 95%confidence interval(CI),we failed to detect a significant relationship between each LMO1 gene SNP(rs110419 A>G,rs4758051 G>A,rs10840002 A>G,rs204938 A>G,and rs2168101 G>T)and CNS tumor risk,respectively.A negative association was also found in the combined effects on these five SNPs and CNS tumor risk.The stratification analysis further demonstrated the individuals with rs204938 AG/GG genotype confer to increased risk of CNS tumor compared with those with an AA genotype in males(OR:1.74,95%CI:1.01-2.98,P=0.046).Interpretation:We concluded that LMO1 gene SNPs may not strong enough to influence the risk of CNS tumor in Chinese children.More studies are required to verify this association. | Huiran Lin Huitong Chen Ao Lin Xiaoping Liu Xiaokai Huang Jingying Zhou Li Yuan Zhenjian Zhuo | 2021 | Pediatric Investigation2021，5，4： | 0 |
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