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| 1 | SEUSS and PIF4 Coordinately Regulate Light and Temperature Signaling Pathways to Control Plant Growth显示文摘 | Junling Huai Xinyu Zhang dialong Li Tingting Ma Ping Zha Yanjun Jing Rongcheng Lin | 2018 | Molecular Plant2018，11，7： | 15 |
| 2 | The Interaction of Population,Industry and Land in Process of Urbanization in China:A Case Study in Jilin Province显示文摘Urbanization is a complex process that covers a wide range of topics, of which population, industry and land urbanization are three important aspects. Jilin Province is an important agricultural province in China. The contradiction between population, industry and land urbanization is especially prominent here, and its coupling development is of great practical significance. In this paper, the coupling degree of population-industry-land urbanization in Jilin Province in 1990, 2000 and 2010 is measured by coupling mode. The spatial pattern of the coupling degree is analyzed by trend surface analysis and global and local spatial autocorrelation. The influence factors and their spatial differentiation are discussed using multiple linear regression(MLR) model and geographic weighted regression(GWR) model. The results show that: 1) the coupling degree of population-industry-land urbanization in Jilin Province is at a low level. Judging from the change of time scale, the urbanization of most research units is becoming more and more coordinated. From the comparison of spatial scales, there is significant spatial difference in the research units of different administrative levels. 2) Judging from the global change trend, the coupling degree of population-industry-land urbanization in the central region is higher than that in western and eastern regions. The coordination of urbanization in the central region is relatively good, and the distribution of the cold and hot spots is basically the same as that overall. 3) The spatial pattern of the coupling degree is related to the cold and hot spot distribution of the influence coefficient of urban population density and per capita urban construction land. The variation of the coupling degree spatial pattern is synchronous with the spatial change of the urban population density influence coefficient. 4) The degree of agglomeration of the urban population is the main factor promoting the coupling pattern of population-industry-land urbanization in Jilin Province, and the extent of its influence is gradually increasing. In addition, the level of city administrative, the efficiency of urban expansion and the capacity of market consumption also have an important influence on the coupling pattern. | LIU Yanjun ZHOU Guolei LIU Degang YU Huisheng ZHU Liyuan ZHANG Jing | 2018 | Chinese Geographical Science2018，28，3： | 12 |
| 3 | A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants显示文摘Developing CRISPR/Cas9-mediated non-transgenic mutants in asexually propagated perennial crop plants is challenging but highly desirable.Here,we report a highly useful method using an Agrobacterium-mediated transient CRISPR/Cas9 gene expression system to create non-transgenic mutant plants without the need for sexual segregation.We have also developed a rapid,cost-effective,and high-throughput mutant screening protocol based on Illumina sequencing followed by high-resolution melting(HRM)analysis.Using tetraploid tobacco as a model species and the phytoene desaturase(PDS)gene as a target,we successfully created and expediently identified mutant plants,which were verified as tetra-allelic mutants.We produced pds mutant shoots at a rate of 47.5%from tobacco leaf explants,without the use of antibiotic selection.Among these pds plants,17.2%were confirmed to be non-transgenic,for an overall non-transgenic mutation rate of 8.2%.Our method is reliable and effective in creating non-transgenic mutant plants without the need to segregate out transgenes through sexual reproduction.This method should be applicable to many economically important,heterozygous,perennial crop species that are more difficult to regenerate. | Longzheng Chen Wei Li Lorenzo Katin-Grazzini Jing Ding Xianbin Gu Yanjun Li Tingting Gu Ren Wang Xinchun Lin Ziniu Deng Richard J.McAvoy Frederick G.Gmitter Jr. Zhanao Deng Yunde Zhao Yi Li | 2018 | Horticulture Research2018，5，1： | 12 |
| 4 | Immobilization of Papain in Biosilica Matrix and Its Catalytic Property显示文摘一个新奇方法通过 papain 导致的一个 biomimetic silicification 过程为 papain 固定被开发。由在一个硅石先锋答案孵化 papain, papain 硅石合成很快形成了, papain 被包含。封装效率和恢复活动分别地是 82.60% 和 83.09% 。与面对另外的猛抛硅石的种类在 biosilica 矩阵使不能调动的酶和 biomolecules 相比,这个 papain 封装过程,一条 biomimetic 途径,由它在温和条件(在中立附近的 pH 和周围的温度) 下面的 autosilification 活动的认识到的高封装效率。而且,包含的 papain 展览提高了热, pH,再循环并且存储稳定性。运动分析证明 biomimetic 硅石矩阵显著地没妨碍底层的集体运输或产品的版本。 | ZHOU Liya WANG Cui JIANG Yanjun GAO Jing | 2013 | Chinese Journal of Chemical Engineering2013，21，6： | 8 |
| 5 | Application of deep learning in ecological resource research:Theories, methods, and challenges显示文摘Ecological resources are an important material foundation for the survival,development,and self-realization of human beings.In-depth and comprehensive research and understanding of ecological resources are beneficial for the sustainable development of human society.Advances in observation technology have improved the ability to acquire long-term,cross-scale,massive,heterogeneous,and multi-source data.Ecological resource research is entering a new era driven by big data.Traditional statistical learning and machine learning algorithms have problems with saturation in dealing with big data.Deep learning is a method for automatically extracting complex high-dimensional nonlinear features,which is increasingly used for scientific and industrial data processing because of its ability to avoid saturation with big data.To promote the application of deep learning in the field of ecological resource research,here,we first introduce the relationship between deep learning theory and research on ecological resources,common tools,and datasets.Second,applications of deep learning in classification and recognition,detection and localization,semantic segmentation,instance segmentation,and graph neural network in typical spatial discrete data are presented through three cases:species classification,crop breeding,and vegetation mapping.Finally,challenges and opportunities for the application of deep learning in ecological resource research in the era of big data are summarized by considering the characteristics of ecological resource data and the development status of deep learning.It is anticipated that the cooperation and training of cross-disciplinary talents may promote the standardization and sharing of ecological resource data,improve the universality and interpretability of algorithms,and enrich applications with the development of hardware. | Qinghua GUO Shichao JIN Min LI Qiuli YANG Kexin XU Yuanzhen JU Jing ZHANG Jing XUAN Jin LIU Yanjun SU Qiang XU Yu LIU | 2020 | Science China Earth Sciences2020，63，10： | 7 |
| 6 | PIF1 and RVE1 form a transcriptional feedback loop to control light-mediated seed germination in Arabidopsis^FA显示文摘The phytochrome B (phyB) photoreceptor plays a major role that inputs light signals to regulate seed dormancy and germination. PHYTOCHROME-INTERACTING FACTOR1 (PIF1) is a key transcription factor repressing phyB-mediated seed germination, while REVEILLE1 (RVE1) factor functions as a curial regulator in controlling both seed dormancy and ger-mination. However, the relationship between the PIF1-and RVE1-modulated signaling pathways remains mostly unknown. Here, we find that PIF1 physically interacts with RVE1. Genetic analysis indicates that RVE1 inhibition on seed germination requires PIF1;re-ciprocally, the repressive effect of PIF1 is partially de-pendent on RVE1. Strikingly, PIF1 and RVE1 directly bind to the promoter and activate the expression of each other. Furthermore, PIF1 and RVE1 coordinately regulate the transcription of many downstream genes involved in abscisic acid and gibberellin pathways. Moreover, PIF1 enhances the DNA-binding ability and transcriptional repression activity of RVE1 in regulating GIBBERELLIN 3-OXIDASE2, and RVE1 promotes PIF1's DNA-binding ability in modulating ABSCISIC ACID-INSENSITIVE3 expression. Thus, this study demon-strates that PIF1 and RVE1 form a transcriptional feedback loop that coordinately inhibits seed germi-nation, providing a mechanistic understanding of how phyB-mediated light signal is transduced to the seeds. | Liwen Yang Zhimin Jiang Yanjun Jing Rongcheng Lin | 2020 | Journal of Integrative Plant Biology2020，62，9： | 7 |
| 7 | Isorhapontigenin protects against doxorubicin-induced cardiotoxicity via increasing YAP1 expression显示文摘As an effective anticancer drug, the clinical limitation of doxorubicin(Dox) is the time-and dose-dependent cardiotoxicity. Yes-associated protein 1(YAP1) interacts with transcription factor TEA domain 1(TEAD1) and plays an important role in cell proliferation and survival. However, the role of YAP1 in Dox-induced cardiomyopathy has not been reported. In this study, the expression of YAP1 was reduced in clinical human failing hearts with dilated cardiomyopathy and Dox-induced in vivo and in vitro cardiotoxic model. Ectopic expression of Yap1 significantly blocked Dox-induced cardiomyocytes apoptosis in TEAD1 dependent manner. Isorhapontigenin(Isor) is a new derivative of stilbene and responsible for a wide range of biological processes. Here, we found that Isor effectively relieved Doxinduced cardiomyocytes apoptosis in a dose-dependent manner in vitro. Administration with Isor(30 mg/kg/day, intraperitoneally, 3 weeks) significantly protected against Dox-induced cardiotoxicity in mice. Interestingly, Isor increased Dox-caused repression in YAP1 and the expression of its target genes in vivo and in vitro. Knockout or inhibition of Yap1 blocked the protective effects of Isor on Dox-induced cardiotoxicity. In conclusion, YAP1 may be a novel target for Dox-induced cardiotoxicity and Isor might be a new compound to fight against Dox-induced cardiotoxicity by increasing YAP1 expression. | Panxia Wang Minghui Wang Yuehuai Hu Jianxing Chen Yanjun Cao Cui Liu Zhongkai Wu Juan Shen Jing Lu Peiqing Liu | 2021 | Acta Pharmaceutica Sinica B2021，11，3： | 7 |
| 8 | Enzyme@silica nanoflower@metal-organic framework hybrids: A novel type of integrated nanobiocatalysts with improved stability显示文摘一个新奇综合 nanobiocatalyst 系统基于 enzyme@silica,有改进稳定性的 nanoflower@metal 器官的框架(enzyme@SNF@ZIF-8 ) 结构第一次被制作。这个系统的通用性作为模型酶用青霉素 G acylase (针网阵列) 和过氧化氢酶(猫) 被验证。多微孔的 ZIF-8 层能被改变 ZIF-8 涂层周期的数字控制,它与不同 ZIF-8 层厚度生产 PGA@SNF@ZIF-8 nanobiocatalysts。在第二个 ZIF-8 涂层周期以后, PGA@SNF@ZIF-8 (2 ) 结构与一同类并且 well-intergrown ZIF-8 层被形成,它拥有优秀机械、化学的稳定性。而且, PGA@SNF@ZIF-8 (2 ) 表演与在硅石 nanoflowers (PGA@SNF ) 上使不能调动的免费针网阵列和针网阵列相比改进了 thermal/storage 稳定性和可重用性。获得的基于猫的 nanobiocatalysts (CAT@SNF@ZIF-8 (2 )) 也显示出优秀催化性能。 | Yingjie Du Jing Gao Huajiao Liu Liya Zhou Ying He Zhihong Huang Yanjun Jiang | 2018 | Nano Research2018，11，8： | 5 |
| 9 | Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E.coli by macromolecular cross-linking agent显示文摘Cross-linked enzyme aggregates(CLEAs) of nitrile hydratase(NHase) ES-NHT-118 from Escherichia coli were prepared by using ammonium sulfate as precipitating agent followed by cross-linking with dextran polyaldehyde for the first time. In this process, egg white was added as protein feeder for facilitating the formation of CLEAs. The optimal conditions of the immobilization process were determined. Michaelis constants(Km) of free NHase and NHase CLEAs were also determined. The NHase CLEAs exhibited increased stability at varied pH and temperature conditions compared to its free counterpart. When exposed to high concentrations of acrylamide, NHase CLEAs also exhibited effective catalytic activity. | Liya Zhou Haixia Mou Jing Gao Li Ma Ying He Yanjun Jiang | 2017 | Chinese Journal of Chemical Engineering2017，25，4： | 5 |
| 10 | Propofol attenuates high glucose-induced P66shc expression in human umbilical vein endothelial cells through Sirt1显示文摘Perioperative hyperglycemia is a common metabolic disorder in clinic settings.Hyperglycemia leads to endothelial inflammation,endothelial cell apoptosis,and dysfunction,thus resulting in endothelial injury.Propofol(2,6-diisopropylphenol)is a widely used intravenous anesthetic in clinic settings.Our previous study indicated that propofol inhibits mitochondrial reactive oxygen species(ROS)production via down-regulation of phosphatase A2(PP2A)expression,inhibition of Ser36-p66shc dephosphorylation and mitochondrial translocation,thus improving high glucose-induced endothelial injury.The expression of p66shc was inhibited by propofol in hyperglycemic human umbilical vein endothelial cells(HUVECs).However,the mechanism by which propofol inhibits p66shc expression in hyperglycemic HUVECs is still obscure.In the present study,we mainly examined how propofol inhibited high glucose-induced p66shc expression in HUVECs.Compared with 5 mM glucose treatment,high glucose increased p66shc expression and decreased sirt1 expression,which was inhibited by propofol treatment.Moreover,EX527(a sirt1 inhibitor)reversed the effect of propofol against high glucose-induced p66shc expression.However,EX527 did not reverse the effects of propofol against high glucose-induced ROS accumulation,endothelial inflammation,and apoptosis.Furthermore,when cells were incubated with propofol,EX527,and FTY720(a PP2A activator)simultaneously,the effects of propofol against high glucose-induced ROS accumulation,inflammation,and apoptosis were reversed.Our results suggested that propofol inhibited high glucose-induced p66shc expression via upregulation of sirt1 expression in hyperglycemic HUVECs.Moreover,propofol protects against high glucose-mediated ROS accumulation and endothelial injury via both inhibition of p66shc expression and dephosphorylation of Ser36-p66shc. | Jing Wang Jie Qi Qichao Wu Hui Jiang Yuehao Yin Yan Huanx Yanjun Zhao Minmin Zhu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 5 |
| 11 | A widely adaptable approach to generate integration-free iPSCs from non-invasively acquired human somatic cells显示文摘 | Zhichao Ding Lina Sui Ruotong Ren Yanjun Liu Xiuling Xu Lina Fu Ruijun Bai Tingting Yuan Ying Hao Weiqi Zhang Huize Pan Wensu Liu Han Yu Concepcion Rodriguez Esteban Xiaobing Yu Ze Yang Jian Li Xiaomin Wang Juan Carlos Izpisua Belmonte Guang-Hui Liu Fei Yi Jing Qu | 2015 | Protein & Cell2015，6，5： | 5 |
| 12 | The Evening Complex and the Chromatin-Remodeling Factor PICKLE Coordinately Control Seed Dormancy by Directly Repressing DOG1 in Arabidopsis显示文摘Primary seed dormancy is acquired during seed development and maturation,which is important for plant fitness and survival.DELAY OF GERMINATION1(DOG1)plays a critical role in inducing seed dormancy.DOG1 expression increases rapidly during seed development,but the precise mechanism underlying this process remains elusive.In this study,we showed that mutants with a loss or reduced function of the chromatin-remodeling factor PICKLE(PKL)exhibit increased seed dormancy.PKL associates with DOG1 chromatin and inhibits its transcription.We found that PKL physically interacts with LUX ARRHYTHMO(LUX),a member of the evening complex(EC)of the circadian clock.Furthermore,LUX directly binds to a specific coding sequence of DOG1,and DOG1 acts genetically downstream of PKL and LUX.Mutations in either LUX or EARLY FLOWERING3(ELF3)encoding another member of the EC led to increased DOG1 expression and enhanced seed dormancy.Surprisingly,these phenotypes were abolished when the parent plants were grown under continuous light.In addition,we observed that loss of function of either PKL or LUX decreased H3K27me3 levels at the DOG1 locus.Taken together,our study reveals a regulatory mechanism in which EC proteins coordinate with PKL to transmit circadian signals for directly regulating DOG1 expression and seed dormancy during seed development. | Ping Zha Shuangrong Liu Yang Li Tingting Ma Liwen Yang Yanjun Jing Rongcheng Lin | 2020 | Plant Communications2020，1，2： | 3 |
| 13 | Studies on pathogen of explosive epidemic disease of shrimp Ⅱ. Purification of pathogen显示文摘 | Wang Jinxing, Liu Changbin, Zhang Hongwei, Zhao Shuangyi, Zhang Yanjun and Zhao Jing (College of Life Sciences, Shandong University, Jinan 250100, China) | 1998 | Acta Oceanologica Sinica1998，17，4： | 3 |
| 14 | PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness显示文摘PHYTOCHROME-INTERACTING FACTORS(PIFs)are a group of basic helix-loop-helix transcription factors that can physically interact with photoreceptors,including phytochromes and cryptochromes.It was previously demonstrated that PIFs accumulated in darkness and repressed seedling photomorphogenesis,and that PIFs linked different photosensory and hormonal pathways to control plant growth and development.In this study,we show that PIFs positively regulate the ABA signaling pathway during the seedling stage specifically in darkness.We found that PIFs positively regulate ABI5 transcript and protein levels in darkness in response to exogenous ABA treatment by binding directly to the G-box motifs in the ABI5 promoter.Consistently,PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness,and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark.Moreover,we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9,and that this interaction is not regulated by ABA.Further analyses showed that PYL8 and PYL9 promote PIF4 protein accumulation in the dark and enhance PIF4 binding to the ABI5 promoter,but negatively regulate PIF4-mediated ABI5 activation.Taken together,our data demonstrate that PIFs interact with ABA receptors to orchestrate ABA signaling in darkness by controlling ABI5 expression,providing new insights into the pivotal roles of PIFs as signal integrators in regulating plant growth and development. | Lijuan Qi Shan Liu Cong Li Jingying Fu Yanjun Jing Jinkui Cheng Hong Li Dun Zhang Xiaoji Wang Xiaojing Dong Run Han Bosheng Li Yu Zhang Zhen Li William Terzaghi Chun-Peng Song Rongcheng Lin Zhizhong Gong Jigang Li | 2020 | Molecular Plant2020，13，3： | 3 |
| 15 | Prediction of major adverse cardiac events is the first critical task in the management of immune checkpoint inhibitor-associated myocarditis显示文摘Dear Editor,The application of immune checkpoint inhibitors(ICIs),anti-tumor immunotherapy unleashing the host immune system to eradicate tumor cells,has revolutionized the treatment of various advanced cancers and showed remarkable anti-tumor effects[1].However,emphasis must be placed on fatal immune-related adverse events(irAEs),especially some fulminant irAEs like ICI-related cardiovascular AEs[2,3].However,due to the low rate of cardiovascular AEs,little is known about the effective methods for evaluating the risk of catastrophic cardiac events in ICI-associated myocarditis and its management[4,5]. | Yan Xu Yanjun Song Xiaoyan Liu Yuequan Shi Yingxian Liu Hao Qian Xiaoxing Gao Minjiang Chen Ruili Pan Jing Zhao Wei Zhong Wei Wu Mengzhao Wang | 2022 | Cancer Communications2022，42，9： | 3 |
| 16 | Quantum nonreciprocality in quadratic optomechanics显示文摘We propose to achieve nonreciprocal quantum control of photons in a quadratic optomechanical(QOM)system based on directional nonlinear interactions.We show that by optically pumping the QOM system in one side,the effective QOM coupling can be enhanced significantly in that side,but not for the other side.This,contrary to the intuitive picture,allows the emergence of a nonreciprocal photon blockade in such optomechanical devices with weak single-photon QOM coupling.Our proposal opens up the prospect of exploring and utilizing quantum nonreciprocal optomechanics,with applications ranging from single-photon nonreciprocal devices to on-chip chiral quantum engineering. | XUNWEI XU YANJUN ZHAO HUI WANG HUI JING AIXI CHEN | 2020 | Photonics Research2020，8，2： | 3 |
| 17 | Electrochemical Determination of Phenylethanolamine A Based on Nafion/MWCNTs/AuNPs Modified Carbon Electrode显示文摘Phenylethanolamine A (豌豆) , -agonist, 被发现去年非法在猪被用作一个生长倡导者,引起中国管理的大注意。这里,一个敏感电气化学的方法为由黄金的固定检测豌豆被开发 nanoparticles (AuNPs ) , multiwalled 碳 nanotubes (MWCNT ) 和一个玻璃质的碳电极(GCE ) 的表面上的 Nafion。Nafion/MWCNTs/AuNPs 电影被扫描电子显微图(SEM ) 和电气化学的阻抗光谱学(EIS ) 描绘。在修改 GCE 的豌豆的电气化学的行为详细被调查。AuNPs, MWCNT 和 Nafion 的合作效果放大豌豆的电气化学的减小信号,并且为豌豆决心导致高敏感。在最佳的条件下面,电气化学的传感器显示出 0.01 ~ 10 的一个宽线性范围(有 0.005 的察觉限制的 mol/L ???????? ? 吗?? | Yanjun Lai Jing Bai Wei Zhu Yuezhong Xian Litong Jin | 2013 | Chinese Journal of Chemistry2013，31，2： | 2 |
| 18 | Nitric oxide enhances the sensitivity of alpaca melanocytes to respond to α-melanocyte-stimulating hormone by up-regulating melanocortin-1 receptor显示文摘 | Yanjun Dong Jing Cao Haidong Wang Jie Zhang Zhiwei Zhu Rui Bai HuanQing Hao Xiaoyan He Ruiwen Fan Changsheng Dong | 2010 | Biochemical and Biophysical Research Communications2010，，4： | 2 |
| 19 | Influential Intensity of Urban Agglomeration on Evolution of Ecoenvironmental Pressure:A Case Study of Changchun,China显示文摘In this paper,we study the interactive relationship between the agglomeration of urban elements and the evolution of eco-environmental pressure.We build an index system for evaluating the agglomeration of urban elements and eco-environmental pressure.Using the entropy method and response intensity model,we analyze how urban elements agglomeration influenced eco-environmental pressure in Changchun from 1990 to 2012,eliciting the changing features and influential factors.Ultimately,we conclude there is a significant interactive relationship between the agglomeration of urban elements and the evolution of eco-environmental pressure in Changchun.This is inferred from the degree of this agglomeration in Changchun having increased since 1990,with the degree of eco-environmental pressure first decreasing and then increasing.Alongside this,the impact of urban elements agglomeration on eco-environmental pressure has changed from negative to positive.The main reasons behind this shift are arguably the rapid growth of urban investment and ongoing urbanization. | LIU Yanjun ZHANG Jing LI Chenggu ZHOU Guolei FU Zhanhui LIU Degang | 2017 | Chinese Geographical Science2017，27，4： | 2 |
| 20 | DNA methylation affects photoperiodic tuberization in potato (Solanum tuberosum L.) by mediating the expression of genes related to the photoperiod and GA pathways显示文摘Overcoming short-day-dependent tuberization to adapt to long-day conditions is critical for the widespread geographical success of potato.The genetic pathways of photoperiodic tuberization are similar to those of photoperiodic flowering.DNA methylation plays an important role in photoperiodic flowering.However,little is known about how DNA methylation affects photoperiodic tuberization in potato.Here,we verified the effect of a DNA methylation inhibitor on photoperiodic tuberization and compared the DNA methylation levels and differentially methylated genes(DMGs)in the photoperiodic tuberization process between photoperiod-sensitive and photoperiod-insensitive genotypes,aiming to dissect the role of DNA methylation in the photoperiodic tuberization of potato.We found that a DNA methylation inhibitor could promote tuber initiation in strict short-day genotypes.Whole-genome DNA methylation sequencing showed that the photoperiod-sensitive and photoperiod-insensitive genotypes had distinct DNA methylation modes in which few differentially methylated genes were shared.Transcriptome analysis confirmed that the DNA methylation inhibitor regulated the expression of the key genes involved in the photoperiod and GA pathways to promote tuber initiation in the photoperiod-sensitive genotype.Comparison of the DNA methylation levels and transcriptome levels identified 52 candidate genes regulated by DNA methylation that were predicted to be involved in photoperiodic tuberization.Our findings provide a new perspective for understanding the relationship between photoperiod-dependent and GA-regulated tuberization.Uncovering the epigenomic signatures of these pathways will greatly enhance potato breeding for adaptation to a wide range of environments. | Yanjun Ai Shenglin Jing Zhengnan Cheng Botao Song Conghua Xie Jun Liu Jun Zhou | 2021 | Horticulture Research2021，8，1： | 1 |
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