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| 1 | Fas ligand expression in colon cancer:A possible mechanism of tumor immune privilege显示文摘AIM: To detect the expression of Fas ligand (FasL) in colon cancer tissues and cell lines and analyze the function of FasL-expressing colon cancer cells in inducing Fas-sensitive T lymphocyte apoptosis. METHODS: Ninety surgically resected colon cancer tissues and 15 hepatic metastasis specimens were investigatedby immunohistochemical method with normal colon mucosa and colon adenoma as control. The relationship between FasL expression and pathologic features was also analyzed.FasL expression of 4 colon cancer cell lines, SW620, Lovo, LS-174T and SW1116, were detected by Western blotting assay. The function of FasL expressed on colon cancer cells was determined by coculture assay with Jurkat T lymphocytes, the apoptotic rate of which was detectedby flow cytometry assay.RESULTS: Fifty-six (62.22%) cases of all the 90 colon cancer tissues and all (100%) the liver metastasis specimens expressed FasL, significantly higher than normal colon mucosa and colonic adenoma. Higher expression of FasL was found in more advanced stage of colon cancer and in cancer tissues with lymphatic or hepatic metastasis.All the colon cancer cell lines were found to express FasL.After coculture with the SW1116 cells for 24 h with an effector: target ratio 10:1, the rate of apoptosis of Jurkat cells rose from 1.9% to 21.0%.CONCLUSION: The expression of FasL is upregulated in colon cancer and the functionally expressed FasL can induce apoptosis of Fas-expressing T lymphocytes. | WeiZhang Er-XunDing QiangWang Da-QiaoZhu JinHe Yu-LiLi Yuan-HeWang | 2005 | World Journal of Gastroenterology2005，11，23： | 6 |
| 2 | Cloning and characterization of a mouse liver-specific gene mfrep-1,up-regulated in liver regeneration显示文摘Human fibrinogen-related protein-1/liver fibrinogen-related protein-1 (HFREP-1/LFIRE-1), a liver-specificprotein, is a member of fibrinogen superfamily that exerts various biological activities. However, the func-tion of HFREP-1/LFIRE-1 in liver remains unknown. Here we isolated its mouse ortholog gene-mousefibrinogen-related protein-1 (mfrep-1), which encoded 314 amino acids, exhibiting 80.4% similarity toHFREP-1/LFIRE-1. Northern blot analysis revealed that 1.2-kb mfrep-1 mRNA was detected selectivelyin mouse liver. To explore the function of MFREP-1, we examined the levels of mfrep-1 mRNA duringregeneration after 70% partial hepatectomy (PHx) in mice. mfrep-1 mRNA increased in the regeneratingliver and reached the first shoulder peak at 2-4 h after PHx. Cycloheximide pretreatment could suppress theinduction of mfrep-1, indicating the up-regulation of this gene need de novo protein synthesis. Its mRNAcontinued to elevate at 6 h thereafter and reached the second peak at 24 h. The enhanced expression ofmfrep-1 maintained high until 72 h and then declined slowly to the basal level. Immunohistochemistryassessment confirmed the up-regulated expression of MFREP-1 protein in parenchymal cells during liverregeneration. These data suggested that MFREP-1 might play an important role in liver regeneration andbe involved in the regulation of cell growth. | JUNYAN HAOYING FEIGU JINHE YuLILI HuIMINLIU YONGHUAXU | 2002 | Cell Research2002，12，5： | 5 |
| 3 | Remediation of heavy metal(loid)s contaminated soils – To mobilize or to immobilize?显示文摘 | Nanthi Bolan Anitha Kunhikrishnan Ramya Thangarajan Jurate Kumpiene Jinhee Park Tomoyuki Makino Mary Beth Kirkham Kirk Scheckel | 2013 | Journal of Hazardous Materials2013，，： | 3 |
| 4 | Transcriptome sequencing assisted discovery and computational analysis of novel SNPs associated with flowering in Raphanus sativus in-bred lines for marker-assisted backcross breeding显示文摘The sequencing of radish genome aids in the better understanding and tailoring of traits associated with economic importance.In order to accelerate the genomics assisted breeding and genetic selection,transcriptomes of 33 radish inbred lines with diverse traits were sequenced for the development of single nucleotide polymorphic(SNP)markers.The sequence reads ranged from 2,560,543,741 bp to 20,039,688,139 bp with the GC(%)of 47.80–49.34 and phred quality score(Q30)of 96.47–97.54%.A total of 4951 polymorphic SNPs were identified among the accessions after stringent filtering and 298 SNPs with efficient marker assisted backcross breeding(MAB)markers were generated from the polymorphic SNPs.Further,functional annotations of SNPs revealed the effects and importance of the SNPs identified in the flowering process.The SNPs were predominantly associated with the four major flowering related transcription factors such as MYB,MADS box(AG),AP2/EREB,and bHLH.In addition,SNPs in the vital flowering integrator gene(FT)and floral repressors(EMBRYONIC FLOWER 1,2,and FRIGIDA)were identified among the radish inbred lines.Further,50 SNPs were randomly selected from 298 SNPs and validated using Kompetitive Allele Specific PCR genotyping system(KASP)in 102 radish inbred lines.The homozygosity of the inbred lines varied from 56 to 96%and the phylogenetic analysis resulted in the clustering of inbred lines into three subgroups.Taken together,the SNP markers identified in the present study can be utilized for the discrimination,seed purity test,and adjusting parental combinations for breeding in radish. | Jinhee Kim Abinaya Manivannan Do-Sun Kim Eun-Su Lee Hye-Eun Lee | 2019 | Horticulture Research2019，6，1： | 2 |
| 5 | Replication and gene expression of mutant hepatitis B virus in a transgenic mouse containing the complete viral genome with mutant sgene显示文摘AIM:To establish the transgenic mouse line harbouring complete hepatitis B virus (HBV) genome with mutant s gene (adr subtype).METHODS: Transgenic mice were generated by microinjecting HBV genome into fertilized eggs. Integration, expression, replication of HBV gene and histological changes in transgenic mice were estimated by genomic DNA PCR, serum DNA PCR, Southern blot, ELISA, HE staining, immunohistochemistry and transmission electron microscopy. Transgenic mice with HBsAg positive in serum were bred and analyzed.RESULTS: A total of 288 eggs survived from microinjections were transplanted into the oviducts of 13 pseudopregnant mice and 49 pups were produced. Twenty-six mice were identified to have the integrated HBV gene. Serum HBsAg and HBeAg were detected in 2 of 43 mice. HBsAg and HBcAg in cytoplasm or nuclei of hepatocytes were detected in 10 mice. Founders with HBsAg in serum were named lineages G145R-15 and G145R-18. Of the 16 F1 offsprings generated by G145R-15 founder, 12 were positive for HBV genome with PCR, 10 were positive for HBsAg and HBcAg with immunohistochemistry and 7 were positive for HBsAg and HBeAg with ELISA. Only 1 of 8 F1 offsprings generated by G145R-18 founder was survived and it was detected positive for HBV genome, HBsAg, HBcAg and HBeAg. Both of the two lineages had some pathological characteristics of mild chronic hepatitis B in the liver, such as swelling of hepatocytes and focal hepatocellular necrosis and parenchymal lymphomononuclear cell infiltrate.CONCLUSION: Transgenic mice harbouring HBV with mutant s gene can be generated. The HBV genes are integrated in the transgenic mice genome and can be expressed, replicated, packaged and excreted. HBV DNA can be stably transmitted in the transgenic mice. | Jun-HuiGe Le-ZhiZhang Jian-XiuLi HongLiu Hui-MinLiu JinHe Yu-ChengYao Yong-JiYang Hong-YuYu Yi-PingHu | 2004 | World Journal of Gastroenterology2004，10，21： | 2 |
| 6 | Melatonin combined with exercise cannot alleviate cerebral injury in a rat model of focal cerebral ischemia/reperfusion injury显示文摘Previous studies have demonstrated that melatonin combined with exercise can alleviate secondary damage after spinal cord injury in rats.Therefore,it is hypothesized that melatonin combined with exercise can also alleviate ischemic brain damage.In this study,adult rats were subjected to right middle cerebral artery occlusion after receiving 10 mg/kg melatonin or vehicle subcutaneously twice daily for 14 days.Forced exercise using an animal treadmill was performed at 20 m/min for 30 minutes per day for 6 days prior to middle cerebral artery occlusion.After middle cerebral artery occlusion,each rat received melatonin combined with exercise,melatonin or exercise alone equally for 7 days until sacrifice.Interestingly,rats receiving melatonin combined with exercise exhibited more severe neurological deficits than those receiving melatonin or exercise alone.Hypoxia-inducible factor 1α mRNA in the brain tissue was upregulated in rats receiving melatonin combined with exercise.Similarly,microtubule associated protein-2 mRNA expression was significantly upregulated in rats receiving melatonin alone.Chondroitin sulfate proteoglycan 4 (NG2) mRNA expression was significantly decreased in rats receiving melatonin combined with exercise as well as in rats receiving exercise alone.Furthermore,neural cell loss in the primary motor cortex was significantly reduced in rats receiving melatonin or exercise alone,but the change was not observed in rats receiving melatonin combined with exercise.These findings suggest that excessive intervention with melatonin,exercise or their combination may lead to negative effects on ischemia/reperfusion-induced brain damage. | Seunghoon Lee Jinhee Shin Minkyung Lee Yunkyung Hong Sang-Kil Lee Youngjeon Lee Tserentogtokh Lkhagvasuren Dong-Wook Kim Young-Ae Yang Kyu-Tae Chang Yonggeun Hong | 2012 | Neural Regeneration Research2012，7，13： | 2 |
| 7 | The effect of RF power on tribological properties of the diamond-like carbon films 显示文摘 | Won Seok Choi Jinhee Heo Ilsub Chung | 2005 | Thin Solid Films2005，475，： | 1 |
| 8 | The coupled thermal mechanical modeling of the inertia friction welding process for inconel718显示文摘 | Bu Wende Liu Jinhe | | 0，，704： | 1 |
| 9 | A dynamic protocol conformance test method显示文摘 | Myungehul Kim Sangjo Yoo Jinhee Park | 2003 | The Journal of Systems and Software2003，67，1： | 1 |
| 10 | A Comparison of Active Adverse Event Surveillance Systems Worldwide显示文摘 | Yu-Lin Huang Jinhee Moon Jodi B Segal | 2014 | Drug Saf2014，37，8： | 1 |
| 11 | Proposition of modeling for constrained mechanical systems based on the vector bond graph显示文摘 | Jinhee Jang Changdoon Han | 1998 | J Franklin Inst1998，335，3： | 1 |
| 12 | Effect of aeration rate on the emission of N 2 O in anoxic–aerobic sequencing batch reactors (A/O SBRs)显示文摘 | Zhen Hu Jian Zhang Shanping Li Huijun Xie Jinhe Wang Tingting Zhang Yiran Li Huayong Zhang | 2009 | Journal of Bioscience and Bioengineering2009，，5： | 1 |
| 13 | Keyword extraction for blogs based on content richness 显示文摘 | JINHEE P JAEKWANG K JEE-HYONG L | 2014 | Journal of information sci- ence2014，40，1： | 1 |
| 14 | The relationship of storage and viability of lily pollen显示文摘 | RheeHyeKyung Lim JinHee ChoHaeRyong etal | 2003 | Acta Horticulturae2003，620，： | 1 |
| 15 | In situ infrared charac- terization during atomic layer deposition of lanthanum oxide显示文摘 | Jinhee K Min D Mathew D H | 2009 | Journal of Physical Chemistry C2009，113，： | 1 |
| 16 | Identification, characterization and expression profiles of Chironomus riparius glutathione S-transferase genes in response to cadmium and silver nanoparticles exposure显示文摘 | Prakash MG Jinhee C | 2011 | Aquat Toxicol2011，101，34： | 1 |
| 17 | Proposition of Modeling for Constrained Mechanical Systems Based on the Vector Bond Graph显示文摘 | JINHEE JANG CHANGSOON HAN | 1998 | J Franklin Inst1998，335，3： | 1 |
| 18 | Compositional Characteristics and Nutritional Evaluation of Different Tissues of Banana Inflorescence (Musa AAA group cv ' baxijiao')显示文摘 | Sheng zhanwu Ma weihong Gao jinhe | | 0，，25： | 1 |
| 19 | Medical Utilization and Cost in Patients with Overlap Syndrome of Chronic Obstructive Pulmonary Disease and Asthma显示文摘 | Chin Kook Rhee Hyoung Kyu Yoon Kwang Ha Yoo Young Sam Kim Sei Won Lee Yong Bum Park Jin Hwa Lee Yuri Kim Kyungjoo Kim Jinhee Kim Yeon Mok Oh Sang Do Lee | 2014 | COPD: Journal of Chronic Obstructive Pulmonary Disease2014，，2： | 1 |
| 20 | Two new constituents of isodon excisus and their evaluation in an apoptosis inhibition assay显示文摘 | CHOONGHWAN L JINHEE K HOJAE L | 2001 | J Nat Prod2001，64，5： | 1 |
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