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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Mobilization of hematopoietic progenitor cells in patients with liver cirrhosis显示文摘AIM:To test the hypothesis that liver cirrhosis is associated with mobilization of hematopoietic progenitor cells. METHODS:Peripheral blood samples from 72 patients with liver cirrhosis of varying etiology were analyzed by flow cytometry.Identified progenitor cell subsets were immunoselected and used for functional assays in vitro. Plasma levels of stromal cell-derived factor-1(SDF-1) were measured using an enzyme linked immunosorbent assay.RESULTS:Progenitor cells with a CD133 + /CD45 + CD14 + phenotype were observed in 61%of th patients.Between 1%and 26%of the peripheral bloo mononuclear cells(MNCs)displayed this phenotype Furthermore,a distinct population of c-kit + progenito cells(between 1%and 38%of the MNCs)could b detected in 91%of the patients.Additionally,18% of the patients showed a population of progenito cells(between 1%and 68%of the MNCs)that wa characterized by expression of breast cancer resistanc protein-1.Further phenotypic analysis disclosed tha the circulating precursors expressed CXC chemokin receptor 4,the receptor for SDF-1.In line with thi finding,elevated plasma levels of SDF-1 were presen in all patients and were found to correlate with th number of mobilized CD133 + progenitor cells. | Ursula M Gehling Marc Willems Kathleen Schlagner Ralf A Benndorf Maura Dandri Jrg Petersen Martina Sterneck Joerg-Matthias Pollok Dieter K Hossfeld Xavier Rogiers | 2010 | World Journal of Gastroenterology2010，16，2： | 4 |
| 3 | Direct Employee Stock Ownership: An Empirical Investment 显示文摘 | James A Brickley Kathleen T Hevert | 1991 | Financial Management1991，，： | 3 |
| 4 | Impact of firm performance expectations on CEO turnover and replacement decisions显示文摘 | Kathleen A Farrell David A Whidbee | 2003 | Journal of Accounting and Economics2003，，1： | 2 |
| 5 | Gut microbiota disturbance during antibiotic therapy: a multi-omic approach显示文摘 | Ana Elena Pérez-Cobas María José Gosalbes Anette Friedrichs Henrik Knecht Alejandro Artacho Kathleen Eismann Wolfgang Otto David Rojo Rafael Bargiela Martin von Bergen Sven C Neulinger Carolin D?umer Femke-Anouska Heinsen Amparo Latorre Coral Barbas Jana | 2013 | Gut2013，，11： | 2 |
| 6 | Dynamic capabilities-What are they?显示文摘 | Kathleen M E Jeffrey A M | 2000 | Strategic Management Journal2000，21，： | 1 |
| 7 | Accounting for small business environme-ntal issues:Parts two:Environmental reporting and disclosure显示文摘 | Kathleen B.H Richard A Jan R.W | | 0，，08： | 1 |
| 8 | Synthetic organo- and polymer-clays: preparation, characterization, and materials applications显示文摘 | Kathleen A Carrado | 2000 | Applied Clay Science2000，，17： | 1 |
| 9 | α - synuclein : A therapeutic target for Parkinson's disease? 显示文摘 | Kathleen A Maguire Z | 2008 | Pharmacol Res2008，58，56： | 1 |
| 10 | Ipriflavone:an important bone-building isoflavone显示文摘 | Kathleen A | 1999 | Alternative Medicine Review1999，4，1： | 1 |
| 11 | Toughening polylactide 显示文摘 | Kelly S Anderson Kathleen MSchreck Marc A Hillmyer | 2008 | Polymer Reviews2008，48，： | 1 |
| 12 | Rapid and sensitive detection of noroviruses by using TaqMan-based one-step reverse transcription-PCR assays and application to naturally contaminated shellfish samples显示文摘 | Jothikumar Narayanan Lowther James A Henshilwood Kathleen | 2005 | Appl Environ Microbiol2005，71，： | 1 |
| 13 | The relationship between obesity and craniofacial structure in obstructive sleep apnea显示文摘 | Kathleen A Takashi Alan A | 1995 | Chest1995，108，： | 1 |
| 14 | New Frontiers for Student Affairs Professionals:Teaching and the First Year Experience显示文摘 | Mary Stuart Hunter Kathleen A Murray | 2007 | New Directions for Student Servises2007，，117： | 1 |
| 15 | Effects of retinoic acid on airspace development and lung collagen in hyperoxia-exposed newborn rats显示文摘 | Kathleen A Meehan V Frank G | 2000 | Pediatr Res2000，48，： | 1 |
| 16 | Nutrient additions generate prolific growth of Lyngbya majuscule (cyanobacteria) in field and bioassay experiments显示文摘 | KATHLEEN S A COLIN R A JAMES W U | 2007 | Harmful Algae2007，6，： | 1 |
| 17 | Regulation of vascular smooth muscle cell differentiation显示文摘 | Eva M Rzucidlo Kathleen A Martin Richard J | 2007 | Jour-nal of Vascular Surgery2007，45，： | 1 |
| 18 | The Effect of CEO Tenure on the Relation Between Firm Performance and Turnover显示文摘 | Sam Allgood Kathleen A Farrell | 2000 | The Journal of Financial Research2000，23，3： | 1 |
| 19 | Fetal state concordance predicts infant state regulation 显示文摘 | Janet A Dipietro Kathleen | 2002 | Early Hum Dev2002，68，1： | 1 |
| 20 | Tourist ' perceptions of environmentally re- sponsible innovations at tourism businesses显示文摘 | Kathleen L A | 2009 | Journal of Sustainable Tourism2009，17，48： | 1 |
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