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| 1 | Chimeric antigen receptor-modified T cells for the immunotherapy of patients with EGFR-expressing advanced relapsed/refractory non-small cell lung cancer显示文摘The successes achieved by chimeric antigen receptor-modified T(CAR-T) cells in hematological malignancies raised the possibility of their use in non-small lung cancer(NSCLC). In this phase I clinical study(NCT01869166), patients with epidermal growth factor receptor(EGFR)-positive(>50% expression), relapsed/refractory NSCLC received escalating doses of EGFR-targeted CAR-T cell infusions. The EGFR-targeted CAR-T cells were generated from peripheral blood after a 10 to 13-day in vitro expansion. Serum cytokines in peripheral blood and copy numbers of CAR-EGFR transgene in peripheral blood and in tissue biopsy were monitored periodically. Clinical responses were evaluated with RECIST1.1 and immune-related response criteria, and adverse events were graded with CTCAE 4.0. The EGFR-targeted CAR-T cell infusions were well-tolerated without severe toxicity. Of 11 evaluable patients, two patients obtained partial response and five had stable disease for two to eight months. The median dose of transfused CAR+ T cells was 0.97×10~7 cells kg^(-1)(interquartile range(IQR), 0.45 to 1.09×10~7 cells kg^(-1)). Pathological eradication of EGFR positive tumor cells after EGFR-targeted CAR-T cell treatment can be observed in tumor biopsies, along with the CAR-EGFR gene detected in tumor-infiltrating T cells in all four biopsied patients. The EGFR-targeted CAR-T cell therapy is safe and feasible for EGFR-positive advanced relapsed/refractory NSCLC. | Kaichao Feng Yelei Guo Hanren Dai Yao Wang Xiang Li Hejin Jia Weidong Han | 2016 | Science China(Life Sciences)2016，59，5： | 62 |
| 2 | Effectiveness of Inactivated COVID-19 Vaccines Against Symptomatic,Pneumonia,and Severe Disease Caused by the Delta Variant:Real World Study and Evidence—China,2021显示文摘Summary What is already known about this topic?Effectiveness of China’s 2 inactivated vaccines(BBIBPCorV and CoronaVac)against pre-Delta severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)variants ranged from 47%to over 90%,depending on the clinical endpoint,and with greater effectiveness against more severe coronavirus disease 2019(COVID-19).During an outbreak in Guangdong,inactivated vaccine effectiveness(VE)against the Delta variant was 70%for symptomatic infection and 100%for severe COVID-19.However,separate or combined VE estimates for the two inactivated vaccines against Delta are not available.What is added by this report?In an outbreak that started in a hospital,VEs of completed primary vaccination with inactivated COVID-19 vaccines against symptomatic COVID-19,COVID-19 pneumonia,and severe COVID-19 caused by the Delta variant were 51%,61%,and 82%.Completed primary vaccination reduced the risk of progressing from mild to moderate or severe COVID-19 by 74%.VE estimates for BBIBP-CorV and CoronaVac or combined vaccination were similar,and partial vaccination was ineffective.What are the implications for public health practice?Completed primary vaccination with either of the 2 inactivated COVID-19 vaccines reduces risk of symptomatic COVID-19,COVID-19 pneumonia,and severe COVID-19 caused by the Delta variant.Completion of the completed primary vaccination with two doses is necessary for protection from Delta. | Dan Wu Yanyang Zhang Lin Tang Fuzhen Wang Ying Ye Chao Ma Hui Zheng Wenzhou Yu Lei Cao Yifan Song Abuduwaili Reyimu Xiaoxiao Zhang Haifeng Wang Yifei Nie Mingxia Lu Muge Qi Jun Li Ruolin Wang Kaichao Yang Changshuang Wang Lawrence Everett Rodewald Geroge Fu Gao Zhijie An Zundong Yin | 2022 | China CDC weekly2022，4，4： | 7 |
| 3 | Phase I study of CAR-T cells with PD-1 and TCR disruption in mesothelin-positive solid tumors显示文摘Programmed cell death protein-1(PD-1)-mediated immunosuppression has been proposed to contribute to the limited clinical efficacy of chimeric antigen receptor T(CAR-T)cells in solid tumors.We generated PD-1 and T cell receptor(TCR)deficient mesothelin-specific CAR-T(MPTK-CAR-T)cells using CRISPR-Cas9 technology and evaluated them in a dose-escalation study.A total of 15 patients received one or more infusions of MPTK-CAR-T cells without prior lymphodepletion.No dose-limiting toxicity or unexpected adverse events were observed in any of the 15 patients.The best overall response was stable disease(2/15 patients).Circulating MPTK-CAR-T cells peaked at days 7–14 and became undetectable beyond 1 month.TCR-positive CAR-T cells rather than TCR-negative CAR-T cells were predominantly detected in effusion or peripheral blood from three patients after infusion.We further confirmed the reduced persistence of TCR-deficient CAR-T cells in animal models.Our results establish the preliminary feasibility and safety of CRISPR-engineered CAR-T cells with PD-1 disruption and suggest that the natural TCR plays an important role in the persistence of CAR-T cells when treating solid tumors. | Zhenguang Wang Na Li Kaichao Feng Meixia Chen Yan Zhang Yang Liu Qingming Yang Jing Nie Na Tang Xingying Zhang Chen Cheng Lianjun Shen Jiaping He Xun Ye Wei Cao Haoyi Wang Weidong Han | 2021 | Cellular & Molecular Immunology2021，18，9： | 7 |
| 4 | An analytical biomarker for treatment of patients with recurrent B-ALL after remission induced by infusion of anti-CD19 chimeric antigen receptor T(CAR-T) cells显示文摘Anti-CD19 chimeric antigen receptor-modified T(CAR-T-19) cells have emerged as a powerful targeted immunotherapy for B-cell lineage acute lymphoblastic leukemia with a remarkable clinical response in recent trials. Nonetheless, few data are available on the subsequent clinical monitoring and treatment of the patients, especially those with disease recurrence after CAR-T-19 cell infusion. Here, we analyzed three patients who survived after our phase I clinical trial and who were studied by means of biomarkers reflecting persistence of CAR-T-19 cells in vivo and predictive factors directing further treatment. One patient achieved 9-week sustained complete remission and subsequently received an allogeneic hematopoietic stem cell transplant. Another patient who showed relapse after 20 weeks without detectable leukemia in the cerebrospinal fluid after CAR-T-19 cell treatment was able to achieve a morphological remission under the influence of stand-alone low-dose chemotherapeutic agents. The third patient gradually developed extensive extramedullary involvement in tissues with scarce immune-cell infiltration during a long period of hematopoietic remission after CAR-T-19 cell therapy. Long-term and discontinuous increases in serum cytokines(mainly interleukin 6 and C-reactive protein) were identified in two patients(Nos. 1 and 6) even though only a low copy number of CAR molecules could be detected in their peripheral blood. This finding was suggestive of persistent functional activity of CAR-T-19 cells. Combined analyses of laboratory biomarkers with their clinical manifestations before and after salvage treatment showed that the persistent immunosurveillance mediated by CAR-T-19 cells would inevitably potentiate the leukemia-killing effectiveness of subsequent chemotherapy in patients who showed relapse after CAR-T-19-induced remission. | Yajing Zhang Wenying Zhang Hanren Dai Yao Wang Fengxia Shi Chunmeng Wang Yelei Guo Yang Liu Meixia Chen Kaichao Feng Yan Zhang Chuanjie Liu Qingming Yang Suxia Li Weidong Han | 2016 | Science China(Life Sciences)2016，59，4： | 6 |
| 5 | Effects of Seed Soaking with Paclobutrazol on Tillering and Physio-logical Characteristics of Sugarcane Seedlings显示文摘In order to study the effects of seed soaking with Paclobutrazol( PP333) on the tillering and physiological characteristics of sugarcane seedlings,sugar cane variety ROC22 was used as experimental material. The effects of seed soaking with different concentrations of PP333 were studied. The tiller number and growth of sugarcane were surveyed at the 6-leaf stage,the physiological indicators such as chlorophyll content,soluble protein content,proline content and peroxidase activity were measured at the 2-leaf,4-leaf,and 6-leaf stages. The results showed that the seed soaking with PP333 can effectively improve the tillering of sugarcane seedlings. The suitable concentration for the tillering of sugarcane and the growth and development of sugarcane was of 50 mg/L; the seed soaking with PP333 significantly increased the chlorophyll,soluble protein,proline,and peroxidase activity of leaves of sugarcane seedlings; the content of chlorophyll and soluble protein in leaves of sugarcane seedlings treated with 90 mg/L PP333 was the highest,and the proline content and peroxidase activity of sugarcane treated with 50 mg/L PP333 were the highest. | Junxian LIU Song LI Fang TAN Xin LIU Yibo HE Kaichao WU Faqian XIONG Limin LIU | 2017 | Asian Agricultural Research2017，9，1： | 1 |
| 6 | Systematical analysis of sludge treatment and disposal technologies for carbon footprint reduction显示文摘This study aims to comprehensively analyze the Greenhouse Gases(GHGs)emissions from current sewage sludge treatment and disposal technologies(buildingmaterial,landfill,land spreading,anaerobic digestion,and thermochemical processes)based on the database of Science Citation Index(SCI)and Social Science Citation Index(SSCI)from 1998 to 2020.The general patterns,spatial distribution,and hotspotswere provided by bibliometric analysis.A comparative quantitative analysis based on life cycle assessment(LCA)put forward the current emission situation and the key influencing factors of different technologies.The effective GHG emissions reduction methods were proposed to mitigate climate change.Results showed that incineration or building materials manufacturing of highly dewatered sludge,and land spreading after anaerobic digestion have the best GHG emissions reduction benefits.Biological treatment technologies and thermochemical processes have great potential for reducing GHGs.Enhancement of pretreatment effect,co-digestion,and newtechnologies(e.g.,injection of carbon dioxide,directional acidification)are major approaches to facilitate substitution emissions in sludge anaerobic digestion.The relationship between the quality and efficiency of secondary energy in thermochemical process and GHGs emission still needs further study.Solid sludge products generated by bio-stabilization or thermochemical processes are considered to have a certain carbon sequestration value and can improve the soil environment to control GHG emissions.The findings are useful for future development and processes selection of sludge treatment and disposal facing carbon footprint reduction. | Yingxin Zhao Zhifan Yang Jiaojiao Niu Zihan Du Conti Federica Zhe Zhu Kaichao Yang Yan Li Baofeng Zhao Thomas Helmer Pedersen Chunguang Liu Mutabazi Emmanuel | 2023 | Journal of Environmental Sciences2023，，6： | 1 |
| 7 | Research on the application of parametric design in gas insulated switchgear显示文摘 | LI Xiaolei YU Kaichao ZHAO Xin | 2010 | Manufacture Information Engineering of China:Academic Edition2010，39，14： | 1 |
| 8 | Dietary supplementation with 2-hydroxy-4-methyl(thio)butanoic acid and DL-methionine improves productive performance,egg quality and redox status of commercial laying ducks显示文摘This experiment aimed to study the effects of supplemental methionine sources,2-hydroxy-4 methyl(thio)butanoic acid(HMTBa)and DL-Methionine(DL-Met),on productive performance,egg quality,and redox status of laying ducks.A total of 792 healthy 25-wk-old Longyan laying ducks with similar body weights were randomly allotted to 11 treatment groups.Each treatment group had 6 replicates of 12 ducks.The trial lasted for 16 wk.Ducks were fed a basal deficient diet(Met:0.24%;Met+Cys:0.51%)or supplemented with DL-Met or HMTBa at 0.05%,0.12%,0.19%,0.26%,and 0.33%of diet,respectively.Compared with the basal diet,supplementation with either DL-Met or HMTBa increased the average egg weight,egg mass,and decreased feed to egg ratio during the whole trial period(P<0.05).Albumen weight and its ratio to total egg weight were increased,but yolk and shell ratio,albumen height,Haugh unit and shell breaking strength were decreased(P<0.05).Dietary DL-Met or HMTBa supplementation increased taurine,methionine,leucine,tryptophan and arginine content,and decreased serine and lysine content in plasma(P<0.05).The redox status of laying ducks was improved by enhancing the glutathione peroxidase and catalase activities,glutathione content and its ratio relative to glutathione(oxidized)content and decreasing malondialdehyde content and increasing mRNA expression of superoxide dismutase-1,glutathione peroxidase-1,hemeoxygenase-1 and nuclear factor-like 2 in liver and ileum with the supplementation of DL-Met or HMTBa(P<0.05).Liver health status measured by average area proportion lipid droplet was improved with supplementation of DL-Met or HMTBa(P<0.05).Villus height and villus height to crypt depth ratio in the ileum and the ileal gene expression of tight junction protein and occludin were increased with DL-Met or HMTBa supplementation(P<0.05).Taken together,these results suggested that the efficacy of dietary supplementation of HMTBa was similar to DL-Met,and it ranged from 98%to 100%for productive performance and egg albumen ratio in laying ducks(25 to 41 wk). | Yanan Zhang Zhiwei Zhuang Tahir Mahmood Yves Mercier Yongyan Jin Xuebing Huang Kaichao Li Shuang Wang Weiguang Xia Shenglin Wang Miao Yu Wei Chen Chuntian Zheng | 2023 | Animal Nutrition2023，，3： | 1 |
| 9 | Research e-infrastructures for open science: The national example of CSTCloud in China显示文摘This paper focuses on research e-infrastructures in the open science era.We analyze some of the challenges and opportunities of cloud-based science and introduce an example of a national solution in the China Science and Technology Cloud(CSTCloud).We selected three CSTCloud use cases in deploying open science modules,including scalable engineering in astronomical data management,integrated Earth-science resources for SDG-13 decision making,and the coupling of citizen science and artificial intelligence(AI)techniques in biodiversity.We conclude with a forecast on the future development of research e-infrastructures and introduce the idea of the Global Open Science Cloud(GOSC).We hope this analysis can provide some insights into the future development of research e-infrastructures in support of open science. | Lili Zhang Jianhui Li Paul F.Uhlir Liangming Wen Kaichao Wu Ze Luo Yude Liu | 2023 | Data Intelligence2023，5，2： | 1 |
| 10 | Towards the selectivity distinction of phenol hydrogenation on noble metal catalysts显示文摘Selective hydrogenation of phenol to cyclohexanone is intriguing in chemical industry.Though a few catalysts with promising performances have been developed in recent years,the basic principle for catalyst design is still missing owing to the unclear catalytic mechanism.This work tries to unravel the mechanism of phenol hydro-genation and the reasons causing the selectivity discrepancy on noble metal catalysts under mild conditions.Results show that different reaction pathways always firstly converge to the formation of cyclohexanone under mild conditions.The selectivity discrepancy mainly depends on the activity for cyclohexanone sequential hy-drogenation,in which two factors are found to be responsible,i.e.the hydrogenation energy barrier and the competitive chemisorption between phenol and cyclohexanone,if the specific co-catalyzing effect of H 2 O on Ru is not considered.Based on the above results,a quantitative descriptor,E b(one/pl)/E a,in which E a can be further correlated to the d band center of the noble metal catalyst,is proposed by the first time to roughly evaluate and predict the selectivity to cyclohexanone for catalyst screening. | Shanjun Mao Zhe Wang Zhirong Chen Kejun Wu Kaichao Zhang Qichuan Li Huihuan Yan Guofeng Lü Guodong Huang Yong Wang | 2023 | Nano Materials Science2023，5，1： | 0 |
| 11 | Design and Validation of a Hardware-In-The-Loop Based Automated Driving Simulation Test Platform显示文摘With the iterative development of autonomous driving technology,selfdriving cars will be one of the most competitive areas in the future.In order to provide students with a better understanding and more comprehensive grasp of autonomous driving technology,a hardware-in-the-loop based autonomous driving simulation test platform has been built.The hardware-in-the-loop system integrates MATLAB/Simulink to build the core control algorithm model,CarMaker simulation software to provide a virtual display interface and vehicle dynamics model,and NVIDIA Jetson to deploy the ECU(Electronic Control Unit)to improve the algorithm power and Logitech G29 series driving simulators providing signal input.It provides a simulation test platform for the development and testing of advanced driver assistance systems,the development and testing of upper layer control algorithms and underlying actuators for autonomous driving,and can provide a teaching and experimental platform for undergraduate students and a development foundation for postgraduate practice. | Kaichao Zheng Xianbin Xue He Li Guoiang Cheng | 2023 | 国际计算机前沿大会会议论文集2023，，1： | 0 |
| 12 | Effects of Seed Soaking with Paclobutrazol on Early Tillering and Endogenous Hormone Contents of Sugarcane Seedlings显示文摘[Objective] The paper was to explore the effect of seed soaking with paclobutrazol( PP333) on early tillering and endogenous hormone content of sugarcane seedlings.[Method] With the sugarcane variety ROC22 as the experimental material,seeds were soaked in different concentrations of PP333. The number and growth of tiller were investigated,and the contents of endogenous hormones IAA,CTK,ABA and GA3 at two-leaf,four-leaf and six-leaf stages of sugarcane seedlings were measured,to study the effects of seed soaking with PP333 on early tillering and endogenous hormone contents of sugarcane seedlings.[Result] Seed soaking with PP333 could effectively improve and advance tillering occurrence of sugarcane seedlings. The suitable PP333 concentration for sugarcane tillering occurrence,growth and development was 50 mg/L. Seed soaking with PP333 significantly increased the CTK and ABA contents in leaves of sugarcane seedlings,and decreased IAA and GA3 contents. [Conclusion]The paper will provide a theoretical basis for application of PP333 in sugarcane production. | Liu Junxian Li Song Tan Fang Xiong Faqian Wang Xiaoman Guo Wenchan Liu Xin He Yibo Wu Kaichao Liu Limin Liu Hongjian Yu Kunxing Dan Ming Lu Manman | 2017 | Plant Diseases and Pests2017，8，1： | 0 |
| 13 | Dual Effects of Interfacial Interaction and Geometric Constraints on Structural Formation of Poly(butylene terephthalate)Nanorods显示文摘When the size of the material is smaller than the size of the molecular chain,new nanostructures can be formed by crystallizing polymers in nanoporous alumina.However,the effect of pore wall and geometric constraints on polymer nanostructures remains unclear.In this study,we demonstrate three new restricted nanostructures{upright-,flat-and tilting-ring}in polybutylene terephthalate(PBT)nanorods prepared from nanoporous alumina.The dual effects of geometrical constraints and interfacial interactions on the formation of PBT nanostructures were investigated for the first time by using X-ray diffraction and Cerius^(2) modeling packages.Under weak constraints,the interaction between pore wall and the PBT rings is dominant and the ring plane tends to be parallel to the pore wall and radiate outward to grow the upright-ring crystals.Surprisingly,in strong 2D confinement,a structural formation reversal occurs and geometrical constraints overpower the effect of pore wall.Rings tend to pile up vertically or obliquely along the long axis of the rod,so the flat-and tilting-ring crystals are predominate in the constrained system.In principle,our study of the nanostructure formation based on the geometrical constraints and the pore wall interfacial effects could provide a new route to manipulate the chain assembly at the nanoscale,further improving the performance of polymer nanomaterial. | Kaichao Wang Ziwei Lai Jiaman Huang Haiying Li Ziying Liang Junhao Liang Xing Ouyang Xiaoguang Li Yan Cao | 2022 | Chinese Journal of Polymer Science2022，40，6： | 0 |
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