3篇
您的检索式：作者名="LIU ZengLei"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Fabrication and characteristic detection of graphene nanoelectrodes显示文摘Graphene has the advantages of high electrical conductivity,high heat conductivity,and low noise,which makes it a potential option for integrated circuits interconnection and nanoelectrodes.In this paper,we present a novel fabrication method for graphene nanoeletrodes with nanogap.First,graphene grown by chemical vapor deposition(CVD)is assembled to a chip with microelectrodes.Second,an atomic force microscopy(AFM)based mechanical cutting method is developed to cut the graphene into nanoribbons and nanoeletrodes with nanogap.Then the electronic property of a single nanodot is characterized using the garphene nanoelectrodes,demonstrating the effectiveness of the graphene nanoelectrodes.The fabricated graphene nanoeletrode pairs can be used as probes to detect single molecule in micro-environment,and show an attractive prospect for future molecular electronics applications. | XIE ShuangXi LIU ZengLei JIAO NianDong TUNG Steve LIU LianQing | 2014 | Science China(Technological Sciences)2014，57，10： | 4 |
| 2 | Antifungal nortriterpene and triterpene glycosides from the sea cucumber Apostichopus japonicus Selenka显示文摘 | Zenglei Wang Hongwei Zhang Weihua Yuan Wei Gong Hua Tang Baoshu Liu Karsten Krohn Ling Li Yanghua Yi Wen Zhang | 2011 | Food Chemistry2011，，1： | 2 |
| 3 | Phosphorylation of PB2 at serine 181 restricts viral replication and virulence of the highly pathogenic H5N1 avian influenza virus in mice显示文摘Influenza A virus(IAV)continues to pose a pandemic threat to public health,resulting a high mortality rate annually and during pandemic years.Posttranslational modification of viral protein plays a substantial role in regulating IAV infection.Here,based on immunoprecipitation(IP)-based mass spectrometry(MS)and purified virus-coupled MS,a total of 89 phosphorylation sites distributed among 10 encoded viral proteins of IAV were identified,including 60 novel phosphorylation sites.Additionally,for the first time,we provide evidence that PB2 can also be acetylated at site K187.Notably,the PB2 S181 phosphorylation site was consistently identified in both IP-based MS and purified virus-based MS.Both S181 and K187 are exposed on the surface of the PB2 protein and are highly conserved in various IAV strains,suggesting their fundamental importance in the IAV life cycle.Bioinformatic analysis results demonstrated that S181E/A and K187Q/R mimic mutations do not significantly alter the PB2 protein structure.While continuous phosphorylation mimicked by the PB2 S181E mutation substantially decreases viral fitness in mice,PB2 K187Q mimetic acetylation slightly enhances viral virulence in mice.Mechanistically,PB2 S181E substantially impairs viral polymerase activity and viral replication,remarkably dampens protein stability and nuclear accumulation of PB2,and significantly weakens IAV-induced inflammatory responses.Therefore,our study further enriches the database of phosphorylation and acetylation sites of influenza viral proteins,laying a foundation for subsequent mechanistic studies.Meanwhile,the unraveled antiviral effect of PB2 S181E mimetic phosphorylation may provide a new target for the subsequent study of antiviral drugs. | Jiao Hu Zixiong Zeng Xia Chen Manyu Zhang Zenglei Hu Min Gu Xiaoquan Wang Ruyi Gao Shunlin Hu Yu Chen Xiaowen Liu Daxin Peng Xiufan Liu | 2024 | Virologica Sinica2024，39，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员