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| 1 | Endothelium-specific SIRT1 overexpression inhibits hyperglycemia-induced upregulation of vascular cell senescence显示文摘The rapidly increasing prevalence of diabetes mellitus worldwide is one of the most serious and challenging health problems in the 21st century.Mammalian sirtuin 1(SIRT1) has been shown to decrease high-glucose-induced endothelial cell senescence in vitro and prevent hyperglycemia-induced vascular dysfunction.However,a role for SIRT1 in prevention of hyperglycemia-induced vascular cell senescence in vivo remains unclear.We used endothelium-specific SIRT1 transgenic(SIRT1-Tg) mice and wild-type(WT) mice to construct a 40-week streptozotocin(STZ)-induced diabetic mouse model.In this mode,42.9% of wild-type(WT) mice and 38.5% of SIRT1-Tg mice were successfully established as diabetic.Forty weeks of hyperglycemia induced significant vascular cell senescence in aortas of mice,as indicated by upregulation of expression of senescence-associated markers including p53,p21 and plasminogen activator inhibitor-1(PAI-1).However,SIRT1-Tg diabetic mice displayed dramatically decreased expression of p53,p21 and PAI-1 compared with diabetic WT mice.Moreover,manganese superoxide dismutase expression(MnSOD) was significantly downregulated in the aortas of diabetic WT mice,but was preserved in diabetic SIRT1-Tg mice.Furthermore,expression of the oxidative stress adaptor p66Shc was significantly decreased in aortas of SIRT1-Tg diabetic mice compared with WT diabetic mice.Overall,these findings suggest that SIRT1-mediated inhibition of hyperglycemia-induced vascular cell senescence is mediated at least partly through the reduction of oxidative stress. | CHEN HouZao WAN YanZhen ZHOU Shuang LU YunBiao ZHANG ZhuQin ZHANG Ran CHEN Feng HAO DeLong ZHAO Xiang GUO ZhiChen LIU DePei LIANG ChihChuan | 2012 | Science China(Life Sciences)2012，55，6： | 14 |
| 2 | Overexpression of a dominant-negative mutant of SIRT1 in mouse heart causes cardiomyocyte apoptosis and early-onset heart failure显示文摘SIRT1,a mammalian ortholog of yeast silent information regulator 2(Sir2),is an NAD+-dependent protein deacetylase that plays a critical role in the regulation of vascular function.The current study aims to investigate the functional significance of deacetylase activity of SIRT1 in heart.Here we show that the early postnatal hearts expressed the highest level of SIRT1deacetylase activity compared to adult and aged hearts.We generated transgenic mice with cardiac-specific expression of a dominant-negative form of the human SIRT1(SIRT1H363Y),which represses endogenous SIRT1 activity.The transgenic mice displayed dilated atrial and ventricular chambers,and died early in the postnatal period.Pathological,echocardiographic and molecular phenotype confirmed the presence of dilated cardiomyopathy.Terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling analysis revealed a greater abundance of apoptotic nuclei in the hearts of transgenic mice.Furthermore,we show that cardiomyocyte apoptosis caused by suppression of SIRT1 activity is,at least in part,due to increased p53acetylation and upregulated Bax expression.These results indicate that dominant negative form of SIRT1(SIRT1H363Y)overexpression in mouse hearts causes cardiomyocyte apoptosis and early-onset heart failure,suggesting a critical role of SIRT1 in preserving normal cardiac development during the early postnatal period. | MU WenLi ZHANG QingJun TANG XiaoQiang FU WenYan ZHENG Wei LU YunBiao LI HongLiang WEI YuSheng LI Li SHE ZhiGang CHEN HouZao LIU DePei | 2014 | Science China(Life Sciences)2014，57，9： | 12 |
| 3 | Isolation,purification and characterization of secondary structure of antifreeze protein from Ammopiptanthus mongo-licus显示文摘Antifreeze protein (AFP) from Ammopiptanthus mongolicus leaves was isolated and purified with DEAE cellulose 52, Sephacryl 300, heat treatment and preparative isotachophoresis. The molecular weight of AFP was 50 ku by SDS-PAGE measurement. The average thermal hysteresis activity (THA) of AFP was 0.35℃ at 5 mg/mL using a differential scanning calorimeter (DSC). This AFP showed heated stability. From circular dichroism (CD) of AFP spectral data from 195-240 nm, a well-defined secondary structure of 11% a-helix, 34% antiparallel β-sheet and 55% random coil for the plant AFP was deduced. | FEI Yunbiao WEI Lingbo GAO Suqin LU Manchun WANG Baohuai LI Zhifen ZHANG Youming SHU Nianhong JIANG Yong WANG Weixiang | 2001 | Chinese Science Bulletin2001，46，6： | 8 |
| 4 | Production of matrix metalloproteinase- 9 by activated human monocytes involves a phosphatidylinositol - 3 kinase/Akt/iKKa/NF -kB pathway 显示文摘 | YunBiao Lu LarryM Wahl | 2005 | Journal of Leukocyte Biology2005，78，： | 1 |
| 5 | p21-activated protein kinase 4(PAK4) interacts with the keratinocyte growth factor receptor and participates in oxidant-induced cell death显示文摘 | Yunbiao Lu Zhong-Zong Pan Yvan Devaux | 2003 | J Biol Chem2003，278，10： | 1 |
| 6 | Morphometrics of Heliolites(Tabulata) from the Late Ordovician, Yushan, Jiangxi, South China显示文摘The Upper Ordovician Xiazhen Formation in the Jiangshan-Changshan-Yushan(JCY) triangle area on the Zhejiang-Jiangxi provincial border, South China, is composed of a mixed carbonate–siliciclastic sequence representing various shallow-water platform environments. Highly diversified and abundant heliolitid coral faunas are recognized in the formation and occur over time and space in certain stratigraphic horizons and various lithofacies. Heliolites has been studied using multivariate morphometric analysis applied to differentiate species and evaluate intraspecific and interspecific variations. Ten morphological characters quantified by examination and measurement from transverse and longitudinal thin sections and statistically tested allowed selection of effective characters to discriminate species. Results of frequency histograms, correlation analysis and principle component analysis indicated that certain characters would be suitable for cluster analysis, which were performed on principle component score matrices obtained from the raw data set coordinated with coralla by all those characters. Five major clusters at a relative distance of five on the resulting dendrogram of Heliolites are regarded as morphospecies thus making valid taxa: Heliolites tashanensis Lin and Chow, 1977; Heliolites caracolica(Lin and Chow, 1977); Heliolites columella(Lin and Chow, 1977); Heliolites waicunensis Lin and Chow, 1977; and H. sinensis irregularis Lin and Chow, 1977. The discrimination of these morphospecies by the cluster analysis is verified by discriminant analyses and descriptive statistics. | DAI Mingjian LIU Lu LEE Dong-Jin PENG Yunbiao MIAO Aisheng | 2015 | Acta Geologica Sinica(English Edition)2015，89，1： | 0 |
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