|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Toxicity of graphene oxide and multi-walled carbon nanotubes against human cells and zebrafish显示文摘Graphene possesses unique physical and chemical properties, which have inspired a wide range of potential biomedical applications. However, little is known about the adverse effects of graphene on the human body and ecological environment. The purpose of our work is to make assessment on the toxicity of graphene oxide (GO) against human cell line (human bone marrow neuroblastoma cell line and human epithelial carcinoma cell line) and zebrafish (Danio rerio) by comparing the toxic effects of GO with its sister, multi-walled carbon nanotubes (MWNTs). The results show that GO has a moderate toxicity to organisms since it can induce minor (about 20%) cell growth inhibition and slight hatching delay of zebrafish embryos at a dosage of 50 mg/L, but did not result in significant increase of apoptosis in embryo, while MWNTs exhibit acute toxicity leading to a strong inhibition of cell proliferation and serious morphological defects in developing embryos even at relatively low concentration of 25 mg/L. The distinctive toxicity of GO and MWNTs should be ascribed to the different models of interaction between nanomaterials and organisms, which arises from the different geometric structures of nanomaterials. Collectively, our work suggests that GO does actual toxicity to organisms posing potential environmental risks and the result is also shedding light on the geometrical structure-dependent toxicity of graphitic nanomaterials. | CHEN LiQiang HU PingPing ZHANG Li HUANG SiZhou LUO LingFei HUANG ChengZhi | 2012 | Science China Chemistry2012，55，10： | 12 |
| 2 | Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction显示文摘 | Xiao-E Jia Ke Ma Tao Xu Lei Gao Shuang Wu Cong Fu Wenjuan Zhang Zhizhang Wang Kaiyu Liu Mei Dong Changbin Jing Chunguang Ren Zhiwei Dong Yi Chen Yi Jin Qiuhua Huang Xing Chang Min Deng Li Li Lingfei Luo Jun Zhu Yongjun Dang Hung-Chun Chang Leonard I Zon Yi Zhou Saijuan Chen Weijun Pan | 2015 | Cell Research2015，25，8： | 8 |
| 3 | Using zebrafish as the model organism to understand organ regeneration显示文摘The limited regenerative capacity of several organs, such as central nervous system(CNS), heart and limb in mammals makes related major diseases quite difficult to recover. Therefore, dissection of the cellular and molecular mechanisms underlying organ regeneration is of great scientific and clinical interests. Tremendous progression has already been made after extensive investigations using several model organisms for decades. Unfortunately, distance to the final achievement of the goal still remains. Recently, zebrafish became a popular model organism for the deep understanding of regeneration based on its powerful regenerative capacity, in particular the organs that are limitedly regenerated in mammals. Additionally, zebrafish are endowed with other advantages good for the study of organ regeneration. This review summarizes the recent progress in the study of zebrafish organ regeneration, in particular regeneration of fin, heart, CNS, and liver as the representatives. We also discuss reasons of the reduced regenerative capacity in higher vertebrate, the roles of inflammation during regeneration, and the difference between organogenesis and regeneration. | SHI WenChao FANG ZhiBing LI Li LUO LingFei | 2015 | Science China(Life Sciences)2015，58，4： | 5 |
| 4 | Synthesis of novel β-amino ketones containing a p-aminobenzoic acid moiety and evaluation of their antidiabetic activities显示文摘The synthesis of two series of β-amino ketones containing a p-aminobenzoic acid moiety (TM-1 and TM-2) using a modified protocol of the Mannich reaction is reported. The molecular structures of a total of tweenty three new target compounds were characterized by 1H NMR, 13C NMR, ESI-MS and HR-MS. Subsequently, their antidiabetic activities were screened in vitro. The α-glucodase inhibition (α-GI) activity of compound 1e reached a remarkable level of 66.50%. The peroxisome proliferatoractivated receptor (PPAR) relative activation activities of six compounds are above 80%, and in particular 2i displays an unprecedentedly high PPAR of 130.91%. The structure-activity relationships of the compounds were established. 2i is also subject to further in-depth investigation. | TANG GuangXia YAN JuFang FAN Li XU Jin SONG XiaoLi JIANG Li LUO LingFei YANG DaCheng | 2013 | Science China Chemistry2013，56，4： | 5 |
| 5 | Hepatocytes in a normal adult liver are derived solely from the embryonic hepatocytes显示文摘In vertebrates,body weight increases many folds as a consequence of body growth from the childhood to adulthood(e.g.,~20 folds for a mouse).Considering the fact that the liver-to-body weight ratio(LBR)stays relatively constant within species(Weglarz and Sandgren,2000;Kan et al.,2009),the cell number in a liver must therefore keep increasing along with the | Ce Gao Zhihui Zhu Yuqi Gao Li Jan Lo Jun Chen Lingfei Luo Jinrong Peng | 2018 | Journal of Genetics and Genomics2018，45，3： | 2 |
| 6 | Zebrafish hhex-null mutant develops an intrahepatic intestinal tube due to de-repression of cdx1b and pdx1显示文摘The hepatopancreatic duct (HPD) system links the liver and pancreas to the intestinal tube and is composed of the extrahepatic biliary duct, gallbladder, and pancreatic duct. Haematopoietically expressed-homeobox (Hhex) protein plays an essential role in the establishment of HPD;however, the molecular mechanism remains elusive. Here, we show that zebrafish hhex-null mutants fail to develop the HPD system characterized by lacking the biliary marker Annexin A4 and the HPD marker sox9b. The hepatobiliary duct part of the mutant HPD system is replaced by an intrahepatic intestinal tube characterized by expressing the intestinal marker fatty acid-binding protein 2a (fabp2a). Cell lineage analysis showed that this intrahepatic intestinal tube is not originated from hepatocytes or cholangiocytes. Further analysis revealed that cdx1b and pdx1 are expressed ectopically in the intrahepatic intestinal tube and knockdown of cdx1b and pdx1 could restore the expression of sox9b in the mutant. Chromatin-immunoprecipitation analysis showed that Hhex binds to the promoters of pdx1 and cdx1b genes to repress their expression. We therefore propose that Hhex, Cdx1b, Pdx1, and Sox9b form a genetic network governing the patterning and morphogenesis of the HPD and digestive tract systems in zebrafish. | Ce Gao Weidong Huang Yuqi Gao Li Jan Lo Lingfei Luo Honghui Huang Jun Chen Jinrong Peng | 2019 | Journal of Molecular Cell Biology2019，11，6： | 2 |
| 7 | The CXCR4-CXCL12 axis promotes T cell reconstitution via efficient hematopoietic immigration显示文摘T cells play a critical role in immunity to protect against pathogens and malignant cells.T cell immunodeficiency is detrimental,especially when T cell perturbation occurs during severe infection,irradiation,chemotherapy,and age-related thymic atrophy.Therefore,strategies that enhance T cell reconstitution provide considerable benefit and warrant intensive investigation.Here,we report the construction of a T cell ablation model in Tg(coro1a:Den NTR)zebrafish via metronidazole administration.The nascent T cells are mainly derived from the hematopoietic cells migrated from the kidney,the functional homolog of bone marrow and the complete recovery time is 6.5 days post-treatment.The cxcr4b gene is upregulated in the responsive hematopoietic cells.Functional interference of CXCR4 via both genetic and chemical manipulations does not greatly affect T lymphopoiesis,but delays T cell regeneration by disrupting hematopoietic migration.In contrast,cxcr4b accelerates the replenishment of hematopoietic cells in the thymus.Consistently,Cxcl12b,a ligand of Cxcr4,is increased in the thymic epithelial cells of the injured animals.Decreased or increased expression of Cxcl12b results in compromised or accelerated T cell recovery,respectively,similar to those observed with Cxcr4b.Taken together,our study reveals a role of CXCR4-CXCL12 signaling in promoting T cell recovery and provides a promising target for the treatment of immunodeficiency due to T cell injury. | Fangying Zhao Yafang Lu Zhifan Li Jiangyong He Nianfei Cui Lingfei Luo Li Li | 2022 | Journal of Genetics and Genomics2022，49，12： | 1 |
| 8 | Ribosome biogenesis protein Urb1 acts downstream of mTOR complex 1 to modulate digestive organ development in zebrafish显示文摘Ribosome biogenesis is essential for the cell growth and division. Disruptions in ribosome biogenesis result in developmental defects and a group of diseases, known as ribosomopathies. Here, we report a mutation in zebrafish urb1, which encodes an essential ribosome biogenesis protein. The urb1 cq31 mutant exhibits hypoplastic digestive organs, which is caused by impaired cell proliferation with the differentiation of digestive organ progenitors unaffected. Knockdown of mtor or raptor leads to similar hypoplastic phenotypes and reduced expression of urb1 in the digestive organs. Overexpression of Urb1 results in overgrowth of digestive organs, and can efficiently rescue the hypoplastic liver and pancreas in the mtor and raptor morphants. Reduced syntheses of free ribosomal subunits and impaired assembly of polysomes are observed in the urb1 mutant as well as in the mtor and raptor morphants, which can be rescued by the Urb1 overexpression. These data demonstrate that Urb1 plays an important role in governing ribosome biogenesis and protein synthesis downstream of mammalian/mechanistic target of rapamycin complex 1(mTORC1), thus regulating the development of digestive organs. Our study indicates the requirement of hyperactive protein synthesis for the digestive organ development. | Jia He Yun Yang Junren Zhang Jinzi Chen Xiangyong Wei Jianbo He Lingfei Luo | 2017 | Journal of Genetics and Genomics2017，44，12： | 1 |
| 9 | Generation of Hematopoietic Stem Cells from Purified Embryonic Endothelial Cells by a Simple and Efficient Strategy显示文摘Recent progress by versatile approaches supports the new hypothesis that multi-potent hematopoietic stem cells(HSCs) are directly formed from a rare population of endothelial cells in mid-gestation mouse embryos.This process is therefore known as the endothelial-tohematopoietic transition(EHT).Nevertheless,there is no functional evidence that documents the HSC transition from purified endothelial cells.In this study,we developed an OP9-DL1-based co-culture system that was able to facilitate the HSC specification and/or expansion in vitro of mouse embryonic day 10.5(E10.5) Tie2^+ cells remarkably.Then,the immunophenotypically defined endothelial cells were harvested by a combination of surface markers(Flk1^+CD31^+CD41^-CD45^-Ter119^-) from the caudal half of E10.0-E11.0 mouse cmbryos.The transplantation of the endothelia/OP9-DL1 co-cultures led to long-term,high-level,multi-lineage,and multi-organ hematopoietic reconstitution in the irradiated adult recipients.The induced HSC activity was initially observed at E10.5,and a significant increase was detected at E11.0,which suggests a temporally specific regulation.Taken together,for the first time,we provide functional evidence showing the HSC potential of purified embryonic endothelial cells,which is indispensable for the emerging EHT concept. Moreover,the newly defined co-culture system will aid the exploration of the key molecules governing the HSC transition from embryonic and even postnatal endothelial cells,which has enormous significance in basic and translational research. | Zhuan Li Fan Zhou Dongbo Chen Wenyan He Yanli Ni Lingfei Luo Bing Liu | 2013 | Journal of Genetics and Genomics2013，40，11： | 1 |
| 10 | EpCAM Is an Endoderm-Specific Wnt Derepressor that Licenses Hepatic Development显示文摘 | Huiqiang Lu Jun Ma Yun Yang Wenchao Shi Lingfei Luo | 2013 | Developmental Cell2013，，5： | 1 |
| 11 | Caudal dorsal artery generates hematopoietic stem and progenitor cells via the endothelial-to-hematopoietic transition in zebrafish显示文摘Zebrafish hematopoietic stem and progenitor cells(HSPCs) originate from the hemogenic endothelium of the ventral wall of the dorsal aorta(DA) through the endothelial-to-hematopoietic transition(EHT) from approximately 30 to 60 hours post fertilization(hpf). However, whether other artery sites can generate HSPCs de novo remains unclear. In this study, using live imaging and lineage tracing, we found that the caudal dorsal artery(CDA) in the caudal hematopoietic tissue directly gave rise to HSPCs through EHT.This process initiated from approximately 60 hpf and terminated at approximately 156 hpf. Compared with that in the DA, fewer EHT events were observed in the CDA. The EHT events in the DA and CDA were similarly regulated by Runx1 but differentially influenced by blood flow(i.e., the EHT frequency in CDA was affected to a lesser extent when circulation was compromised in the tnnt2a^(-/-)mutant). Therefore,the whole artery, including both DA and CDA, was endowed with the ability to produce HSPCs during a much longer time period. Coincidently, the lineage tracing results indicated that adult hematopoietic cells originated from the embryonic endothelium, and those produced later preferentially colonized the adult thymus. Collectively, our study revealed that the CDA serves as an additional source of hematopoiesis, and it shows similar but not identical properties with the DA. | YANDong Zhan Youkui Huang Jingying Chen Zigang Cao Jianbo He Jingjing Zhang Honghui Huang Hua Ruan Lingfei Luo Li Li | 2018 | Journal of Genetics and Genomics2018，45，6： | 0 |
| 12 | mTORC1 signaling pathway regulates tooth repair显示文摘Tooth germ injury can lead to abnormal tooth development and even tooth loss,affecting various aspects of the stomatognathic system including form,function,and appearance.However,the research about tooth germ injury model on cellular and molecule mechanism of tooth germ repair is still very limited.Therefore,it is of great importance for the prevention and treatment of tooth germ injury to study the important mechanism of tooth germ repair by a tooth germ injury model.Here,we constructed a Tg(dlx2b:Dendra2-NTR)transgenic line that labeled tooth germ specifically.Taking advantage of the NTR/Mtz system,the dlx2b+tooth germ cells were depleted by Mtz effectively.The process of tooth germ repair was evaluated by antibody staining,in situ hybridization,Ed U staining and alizarin red staining.The severely injured tooth germ was repaired in several days after Mtz treatment was stopped.In the early stage of tooth germ repair,the expression of phosphorylated 4E-BP1 was increased,indicating that mTORC1 is activated.Inhibition of mTORC1 signaling in vitro or knockdown of mTORC1 signaling in vivo could inhibit the repair of injured tooth germ.Normally,mouse incisors were repaired after damage,but inhibition/promotion of mTORC1 signaling inhibited/promoted this repair progress.Overall,we are the first to construct a stable and repeatable repair model of severe tooth germ injury,and our results reveal that mTORC1 signaling plays a crucial role during tooth germ repair,providing a potential target for clinical treatment of tooth germ injury. | Honghong Liu Yu Yue Zhiyun Xu Li Guo Chuan Wu Da Zhang Lingfei Luo Wenming Huang Hong Chen Deqin Yang | 2023 | International Journal of Oral Science2023，15，1： | 0 |
| 13 | Alterations in immune cell heterogeneities in the brain of aged zebrafish using single-cell resolution显示文摘Immunocytes,including the microglia,are crucial in the neurodegenerative process in old people.However,the understanding regarding microglia heterogeneity and other involved immunocytes remains elusive.We analyzed 26,456 immunocytes from 12-and 26-month-old zebrafish brains at single-cell resolution.Microglia and T lymphocytes were detected in the brain at both time points.Two types of microglia were annotated,namely,ac+microglia and xr+microglia,which were clustered into subsets 1,2,3,4,5,and subsets 6,7,8,9,respectively.Diversified microglia predominated the adult brains and cooperated with T cells to perform the functions of immune response and neuronal nutrition.We validated the specific microglia markers.The novel transgenic lines,Tg(lgals3bpb:e GFP)and Tg(apoc1:e GFP),were created,which faithfully labeled ac+microglia and served as valuable labeling tools.However,the microglia population reduced while T cells of six subtypes intriguingly increased to serve as the primary immune cells in aged brains.Unlike in 12-month-old brains,T cells,together with microglia,exhibited a coordinated signature of inflammation in the 26-month-old brains.Our findings revealed the immunocytes atlas in aged zebrafish brains.It implied the involvement of microglia and T cells in the progression of neurodegeneration in aging. | Jiangyong He Fangying Zhao Bingyue Chen Nianfei Cui Zhifan Li Jie Qin Lingfei Luo Congjian Zhao Li Li | 2023 | Science China(Life Sciences)2023，66，6： | 0 |
| 14 | GoldenFish:a rapid and efficient system to customize constructs for zebrafish transgenesis显示文摘Transgenesis, which inserts exogenous DNA into animal genomes, is a widely used technique. Traditionally, the constructs for transgenesis are generated by step-by-step subcloning of DNA fragments, which requires multiple steps depending on the construct complexity. To overcome the limitation, advanced tools such as Gateway cloning (Hartley et al., 2000;Kwan et al., 2007), In-Fusion cloning (Sleight et al., 2010), and Gibson assembly (Gibson et al., 2009) have been developed. However, due to their ligation characteristics, no systematic method for transgenesis has been developed. ‘Golden Gate’ cloning first appeared in 2008, which is a widely used DNA assembly method (Engler et al., 2008, 2009). Here, we take zebrafish transgenesis as an example and develop a standardized system called GoldenFish, which is based on Golden Gate cloning. It can customize transgenic constructs in one step and can be applied to multiple types of transgenesis such as one gene driven by one promoter, multiple genes driven by one promoter, and multiple genes respectively driven by multiple promoters, significantly reducing working time. | Zhanmei Jiang Lirong Huang Jieqiong Zhao Yanfeng Li Jianlong Ma Rui Ni Qifen Yang Lingfei Luo Yun Yang Jingying Chen | 2022 | Journal of Molecular Cell Biology2022，14，12： | 0 |
| 15 | Zebrafish ppp1r21 mutant as a model for the study of primary biliary cholangitis显示文摘Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease that progresses to fibrosis and cirrhosis, resulting from the gradual destruction of intrahepatic bile ducts. Exploring genetic variants associated with PBC is essential to understand the pathogenesis of PBC. Here we identify a zebrafish balloon dog (blg) mutant with intrahepatic bile duct branching defects, exhibiting several key pathological PBC-like features, including immunodominant autoantigen PDC-E2 production, cholangiocyte apoptosis, immune cell infiltration, inflammatory activation, and liver fibrosis. blg encodes the protein phosphatase 1 regulatory subunit 21 (Ppp1r21), which is enriched in the liver and its peripheral tissues and plays a vital role in the early intrahepatic bile duct formation stage. Further studies show an excessive activation of the PI3K/AKT/mTOR pathway in the hepatic tissues in the mutant, while treatment with the pathway inhibitor LY294002 and rapamycin partially rescues intrahepatic bile duct branching defects and alleviates the PBC-like symptoms. These findings implicate the potential role of the Ppp1r21-mediated PI3K/AKT/mTOR pathway in the pathophysiology of PBC. | Chaoying Wu Wenfeng Zhang Yiyu Luo Chaoqing Cheng Xinjuan Wang Yan Jiang Shuang Li Lingfei Luo Yun Yang | 2023 | Journal of Genetics and Genomics2023，50，12： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员