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| 1 | An improved binarization algorithm of wood image defect segmentation based on non-uniform background显示文摘In this study,an image binarization optimization algorithm,based on local threshold algorithms,is proposed because global and traditional local threshold segmentation algorithms cannot effectively address the problems of nonuniform backgrounds of wood defect images.The proposed algorithm calculates the threshold by the mean,standard deviation and the extreme value of the window.The results indicate that this modified algorithm enhances the image segmentation for wood defect images on a complex background,which is much superior to the global threshold algorithm and the Bernsen algorithm,and slightly better than the Niblack algorithm and Sauvola algorithm.Compared with similar models,the algorithm proposed in this paper has higher segmentation accuracy,as high as 92.6%for wood defect images with a complex background. | Wei Luo Liping Sun | 2019 | Journal of Forestry Research2019，30，4： | 13 |
| 2 | (E)-Nerolidol is a volatile signal that induces defenses against insects and pathogens in tea plants显示文摘Plants release large amounts of volatile organic compounds(VOCs)in response to attackers.Several VOCs can serve as volatile signals to elicit defense responses in undamaged tissues and neighboring plants,but many questions about the ecological functions of VOCs remain unanswered.Tea plants are impacted by two harmful invaders,the piercing herbivore Empoasca(Matsumurasca)onukii Matsuda and the pathogen Colletotrichum fructicola.To determine the VOC signals in tea,we confirmed CsOPR3 as a marker gene and set up a rapid screening method based on a 1.51 kb CsOPR3 promoter fused with aβ-glucuronidase(GUS)reporter construct(OPR3p::GUS)in Arabidopsis.Using this screening system,a terpenoid volatile(E)-nerolidol was identified as a potent signal that elicits plant defenses.The early responses triggered by(E)-nerolidol included the activation of a mitogen-activated protein kinase and WRKY,an H2O2 burst,and the induction of jasmonic acid and abscisic acid signaling.The induced plants accumulated high levels of defenserelated chemicals,which possessed broad-spectrum anti-herbivore or anti-pathogen properties,and ultimately triggered resistance against Empoasca onukii and Colletotrichum fructicola in tea.We propose that these findings can supply an environmentally friendly management strategy for controlling an insect pest and a disease of tea plants. | Shenglong Chen Liping Zhang Xiaoming Cai Xin Li Lei Bian Zongxiu Luo Zhaoqun Li Zongmao Chen Zhaojun Xin | 2020 | Horticulture Research2020，7，1： | 12 |
| 3 | GIS-based detection of land use transformation in the Loess Plateau: A case study in Baota District, Shaanxi Province, China显示文摘在过去的十年期间,大努力被作了在黄土高原增加陆地使用转变,特别为减少由植被恢复措施的土壤侵蚀。Grain-for-Green 工程(GFG ) 是在中国的最大的生态的康复程序,它为西方中国的生态地易碎的区域在植被恢复和持续开发上有积极影响。为三个次时期(2000, 2005 和 2010 ) 基于 Landsat TM/ETM 图象,这研究使用了 GIS 技术和一个山斜坡揭示在 Baota 区域把斜坡农田还给草地或树林的时间空间的进化模式的分析模型, Shaanxi 的 Yanan 城市省。结果显示出那:(1 ) 从 2000 ~ 2010,农田的区域在约 35,030 减少了哈,而草地,树林和构造土地增加了,它是在所有陆地使用类型之中的最大的减少,哪个 26,380 很快扩展的草地哈。(2 ) 陆地使用动力学的年度变化率在时期期间是 1.98% 20002010,哪个率为 20002005 时期是 1.05% 并且 2.92% 为 20052010 时期分别地。在过去的十年,把农田还给树林或牧场是增加的草地和树林的主要来源,并且农田的减小分别地在 97.39% 和 85.28% 在草地和树林贡献了增加。(3 ) 当地面斜坡增加,农田减少了,树林和草地显著地增加了。有从 15 潰獳獥楳湯的一个斜坡的区域? | GUO Liying DI Liping LI Gang LUO Qiyou GAO Mingjie | 2015 | Journal of Geographical Sciences2015，25，12： | 11 |
| 4 | A critical review on the application of biochar in environmental pollution remediation:Role of persistent free radicals(PFRs)显示文摘Biochar as an emerging carbonaceous material has exhibited a great potential in environmental application for its perfect adsorption ability.However,there are abundant persistent free radicals(PFRs)in biochar,so the direct and indirect PFRs-mediated removal of organic and inorganic contaminants by biochar was widely reported.In order to comprehend deeply the formation of PFRs in biochar and their interactions with contaminants,this paper reviews the formation mechanisms of PFRs in biochar and the PFRs-mediated environmental applications of biochar in recent years.Finally,future challenges in this field are also proposed.This review provides a more comprehensive understanding on the emerging applications of biochar from the viewpoint of the catalytic role of PFRs. | Kun Luo Ya Pang Dongbo Wang Xue Li Liping Wang Min Lei Qi Huang Qi Yang | 2021 | Journal of Environmental Sciences2021，33，10： | 8 |
| 5 | Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference显示文摘Mesenchymal stem cells(MSCs)are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic,chondrogenic and adipogenic lineages.We previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molecular mechanism through which BMP9 regulates osteogenic differentiation remains to be fully understood.Here,we seek to develop a recombinant adenovirus system to optimally silence mouse BMP9 and then characterize the important role of BMP9 in osteogenic differentiation of MSCs.Using two different siRNA bioinformatic prediction programs,we design five siRNAs targeting mouse BMP9(or simB9),which are expressed under the control of the converging H1 and U6 promoters in recombinant adenovirus vectors.We demonstrate that two of the five siRNAs,simB9-4 and simB9-7,exhibit the highest efficiency on silencing exogenous mouse BMP9 in MSCs.Furthermore,simB9-4 and simB9-7 act synergistically in inhibiting BMP9-induced expression of osteogenic markers,matrix mineralization and ectopic bone formation from MSCs.Thus,our findings demonstrate the important role of BMP9 in osteogenic differentiation of MSCs.The characterized simB9 siRNAs may be used as an important tool to investigate the molecular mechanism behind BMP9 osteogenic signaling.Our results also indicate that recombinant adenovirus-mediated expression of siRNAs is efficient and sustained,and thus may be used as an effective delivery vehicle of siRNA therapeutics. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C.Haydon Michael J.Lee Russell R.Reid Jennifer Moriatis Wolf Qiong Shi Hue H.Luu Tong-Chuan He Yaguang Weng | 2018 | Genes & Diseases2018，5，2： | 8 |
| 6 | Ablation of C/SiC-HfC composite prepared by precursor infiltration and pyrolysis in plasma wind tunnel显示文摘Carbon fiber reinforced silicon carbide-hafnium carbide(C/SiC-HfC)composite was prepared by precursor infiltration and pyrolysis process.Then,ablation behavior of C/SiC-HfC was evaluated in plasma wind tunnel.It was found that oxide layer formed during ablation significantly influenced the surface temperature.Formation of dense HfO2 SiO2 layer under low heat flux led to stable surface temperature.Silica(SiO2)on the surface was gradually consumed when heat flux increased,resulting in conversion of HfO2-SiO2 on the surface to HfO2.Converted HfO2 with high catalytic cofficient absorbed more energy,causing gradual increase in the surface temperature.Formed oxide layer was destroyed at high heat flux and high stagnation point pressure.After carbon fiber lost the protection of HfO2-SiO2 layer,it burned immediately,leading to surface temperature jump. | Liuyang DUAN Lei LUO Liping LIU Yiguang WANG | 2020 | Journal of Advanced Ceramics2020，9，3： | 7 |
| 7 | CONTINUUM TOPOLOGY OPTIMIZATION FOR MONOLITHIC COMPLIANT MECHANISMS OF MICRO-ACTUATORS显示文摘为分布式的顺从的机制的结构的拓扑学优化的一个多客观的计划处于 MEMS 状况微致动器在这个工作,机械灵活性和结构的僵硬在是看作客观功能的两个被介绍。这样开发的 Thecompliant 微观机构不能仅仅提供足够的产量工作而且有足够的刚硬抵抗反应力量并且当保持 thework 片时,维持它的形状。过滤途径的密度也被建议绞链在产生 mechanisms.SIMP 发生消除象棋盘,连接的网孔相关性和一个节点那样的数字不稳定性被用作插值计划显示材料模量的依赖调整 onelement 的密度。顺序的凸的编程方法例如动人的渐近线(MMA ) 的方法,被用来解决优化问题。介绍方法论的确认被一个典型数字例子表明。 | Luo Zhen Du Yixian Chen Liping Yang Jingzhou Karim Abdel-Malek | 2006 | Acta Mechanica Solida Sinica2006，19，1： | 6 |
| 8 | NECK LEAF 1, a GATA type transcription factor, modulates organogenesis by regulating the expression of multiple regulatory genes during reproductive development in rice显示文摘在 monocot 米饭种类 Oryza sativa L. ,最惹人注目的词法过程之一在繁殖开发期间是有上面的 internodes (合众国际社) 的顺序的延伸的圆锥花序开发的同时发生。阐明内在的分子的机制,我们克隆米饭基因颈叶 1 (NL1 ) ,什么时候变异,它在 flowering 时间,有簇叶丛生的苞的更小的圆锥花序和反常合众国际社延伸模式导致延期。NL1 基因与一个单个锌手指领域,和它的抄本编码一个 GATA 类型抄写因素在苞 primordia 主要被检测,它通常在野类型的植物堕落。在转基因的植物的 NL1 的 Overexpression 经常产生严重生长延迟,不太植物的 phytomers 和更小的叶子,建议 NL1 起在器官区别的一个重要作用。PLASTOCHRON1 (PLA1 ) 的新奇变异的等位基因,知道在调整叶开始起一个关键作用的基因,在这研究被识别。基因分析表明了在 nl1 和 pla1 之间的一个相互作用,与 PLA1 在上游的代理的 NL1。表示水平和 PLA1 的空间模式被发现在 nl1 被改变变异。而且, flowering, Hd3a 和 OsMADS1 的二个管理者的表示,也在 nl1 异种被影响。根据这些调查结果,我们建议 NL1 是通过在米饭在繁殖开发期间调整 PLA1 和另外的规章的基因的表示调制并且协调 organogenesis 的一个内在的因素。 | Liping Wang Hengfu Yin Qian Qian Jun Yang Chaofeng Huang Xiaohe Hu Da Luo | 2009 | Cell Research2009，19，5： | 6 |
| 9 | An Efficient Real-Time Fault-Tolerant Scheduling Algorithm Based on Multiprocessor Systems显示文摘In the context of real-time fault-tolerant scheduling in multi- processor systems, Primary-backup scheme plays an important role. A backup copy is always preferred to be executed as passive backup copy whenever possible because it can take the advantages of backup copy de-allocation technique and overloading technique to improve schedula- bility. In this paper, we propose a novel efficient fault-tolerant rate- monotonic best-fit algorithm efficient fault-tolerant rate-monotonic best-fit (ERMBF) based on multiprocessors systems to enhance the schedulability. Unlike existing scheduling algorithms that start schedul- ing tasks with only one processor. ERMBF pre-allocates a certain amount of processors before starting scheduling tasks, which enlarge the searching spaces for tasks. Besides, when a new processor is allocated, we reassign the task copies that have already been assigned to the exist- ing processors in order to find a superior tasks assignment configuration. These two strategies are all aiming at making as many backup copies as possible to be executed as passive status. As a result, ERMBF can use fewer processors to schedule a set of tasks without losing real-time and fault-tolerant capabilities of the system. Simulation results reveal that ERMBF significantly improves the schedulability over existing, compa- rable algorithms in literature. | YANG Fumin LUO Wei PANG Liping | 2007 | Wuhan University Journal of Natural Sciences2007，12，1： | 6 |
| 10 | A nonsynonymous SNP in human cytosolic sialidase in a small Asian population results in reduced enzyme activity: potential link with severe adverse reactions to oseltamivir显示文摘oseltamivir 的使用,广泛地是的 stockpiled 为在可能的鸟的流行性感冒的使用的药之一流行,被报导了与 neuropsychiatricdisorders 和严重皮肤反应被联系,首先在日本。这里,我们在 dbSNP 数据库识别了非同义的 SNP (单个核苷酸多型性) , R41Q 在 humancytosolic sialidase 的酶的活跃地点附近,是 oseltamivir 的目标的病毒 neuraminidase 的一个相当或相同事物。在欧洲、非洲的美国人口的 9.29% 亚洲人口和没有的这 SNPoccurred。Ourstructural 分析和 Ki 大小使用试管内 sialidase 试金显示这 SNPcould 增加人的 sialidase 的非计划中的有约束力的亲密关系到 oseltamivir 羧化物, oseltamivir 的活跃形式,因此减少 sialidase 活动。另外,这 SNP 自己与一项内在地更低的 sialidase 活动导致酶,由它的增加的 Km 证明 Vmax 珍视并且减少。理论上,到有这 SNP 的人的 oseltamivir 的管理可能进一步减少他们的 sialidase 活动。我们注意 oseltamivir 的 reportedneuropsychiatric 副酌和人的 sialidase-relateddisorders 的已知的症状的类似。我们建议这个充实亚洲人的 sialidase 变化由 SNP 引起了,在同型结合的形式可能,可以与对 oseltamivir 的某些严重不利反应被联系。 | Chuan-Yun Li Quan Yu Zhi-Qiang Ye Ying Sun Quanyuan He Xiao-Mo Li Wuxue Zhang Jingchu Luo Xiaocheng Gu Xiaofeng Zheng Liping Wei | 2007 | Cell Research2007，17，4： | 5 |
| 11 | OSCILLATION THEOREM TO SYSTEMS OF IMPULSIVE NEUTRAL DELAY PARABOLIC PARTIAL DIFFERENTIAL EQUATIONS显示文摘In this paper,we study the oscillation of solutions to the systems of impul- sive neutral delay parabolic partial differential equations.Under two different boundary conditions,we obtain some sufficient conditions for oscillation of all solutions to the systems. | Luo Liping Ouyang Zigen | 2007 | Annals of Differential Equations2007，23，3： | 5 |
| 12 | The efficacy and safety of nimodipine in acute ischemic stroke patients with mild cognitive impairment: a double-blind, randomized,placebo-controlled trial显示文摘Nimodipine might be effective in subcortical vascular dementia(VaD). Its benefit in preventing further cognitive decline in patients with acute ischemic stroke(AIS) and vascular mild cognitive impairment(VaMCI) remains to be established. In this multicenter, double-blind trial, we randomly assigned 654 eligible patients to nimodipine 30 mg three times a day or placebo. The primary outcome was any cognitive decline defined by the changes on the Mini-Mental State Examination(DMMSE à3) or vascular AD assessment scale cognitive subscale(DADAS-cog ! 4) at 6 months. Secondary outcomes included any distribution shift of DADAS-cog, DMMSE or cognitive improvement defined by DADAS-cog à2, or DMMSE ! 0. The primary outcome in the nimodipine group and placebo group were similar for DMMSE à3(4.18% and 7.22%, respectively, P = 0.15) and DADAS-cog ! 4(8.36% and 8.93% respectively,P = 0.88). The distribution shift of DADAS-cog and DMMSE differed significantly between the two groups(P = 0.03 and P = 0.05 respectively). Cognitive improvement occurred in 55.4% in the nimodipine group and 43.6% in the placebo group measured by DADAS-cog à2(Odds Ratio, 1.54; 95% confidence interval[CI] 1.10–2.14, P < 0.01) or 84.0% and 74.6% respectively by DMMSE ! 0(Odds Ratio, 1.79; 95% CI 1.18–2.70, P < 0.01). Nimodipine was associated with better cognitive function in the memory domain. The adverse events rate was similar in two groups. This study is registered with ClinicalTrials.gov,NCT01220622. Nimodipine did not show benefit to prevent cognitive decline in AIS patients with VaMCI, but improved cognition moderately, especially measured in the memory domain. | Huaguang Zheng Yilong Wang Anxin Wang Hao Li David Wang Xingquan Zhao Penglian Wang Haipeng Shen Lijun Zuo Yuesong Pan Zixiao Li Xia Meng Xianwei Wang Weixiong Shi Yi Ju Liping Liu Kehui Dong Chunxue Wang Rubo Sui Rong Xue Xiaoping Pan Xiaoyua Niu Benyan Luo Yi Sui Huali Wang Tao Feng Yongjun Wang | 2019 | Science Bulletin2019，64，2： | 5 |
| 13 | A New Recommender System Using Context Clustering Based on Matrix Factorization Techniques显示文摘Recommender system can efficiently alleviate the information overload problem, but it has been trapped in the recommendation accuracy. We proposed a new recommender system which based on matrix factorization techniques. More factors including contextual information, user ratings and item feature are all taken into consideration. Meanwhile the k-modes algorithm is used to reduce the complexity of matrix operations and increase the relevance of the user-item ratings sub-matrix.Compared with several ma jor existing recommendation approaches, extensive experimental evaluation on publicly available dataset demonstrates that our method enjoys improved recommendation accuracy. | ZHENG Xiaoyao LUO Yonglong SUN Liping CHEN Fulong | 2016 | Chinese Journal of Electronics2016，25，2： | 4 |
| 14 | Dynamic temperature prediction of electronic equipment under high altitude long endurance conditions显示文摘Unmanned Aerial Vehicle(UAV) is developing towards the direction of High Altitude Long Endurance(HALE). This will have an important influence on the stability of its airborne electronic equipment using passive thermal management. In this paper, a multi-node transient thermal model for airborne electronic equipment is set up based on the thermal network method to predict their dynamic temperature responses under high altitude and long flight time conditions. Some relevant factors are considered into this temperature prediction model including flight environment,radiation, convection, heat conduction, etc. An experimental chamber simulating a high altitude flight environment was set up to survey the dynamic thermal responses of airborne electronic equipment in a UAV. According to the experimental measurement results, the multi-node transient thermal model is verified without consideration of the effects of flight speed. Then, a modified way about outside flight speed is added into the model to improve the temperature prediction performance. Finally, the corresponding simulation code is developed based on the proposed model. It can realize the dynamic temperature prediction of airborne electronic equipment under HALE conditions. | Liping PANG Miao ZHAO Kun LUO Yongli YIN Zhou YUE | 2018 | Chinese Journal of Aeronautics2018，31，6： | 4 |
| 15 | Application research of a novel designed peptide as a potential carrier显示文摘While a great deal of research has focused on the application of full-sequence ionic complementary peptide, detection of the capability of half-sequence ionic complementary peptide such as drug carriers, is rarely reported. This paper presents that the half-sequence ionic complementary peptide P9 (AC-Pro- Ser-Phe-Asn-Phe-Lys-Phe-Glu-Pro-NH2) can successfully stabilize a model hydrophobic drug pyrene in the aqueous solution. Soybean lecithin vesicles were used to mimic plasma membranes. Fluorescence data show that the pyrene is presented in the crystalline form when stabilized by P9 solution, and molecularly migrated from its peptide encapsulations into the membrane bilayers when the suspension is mixed with lipidosome vesicles. Slower release was observed when thicker coating was applied onto pyrene, which could be to control the wall thickness coating the cargo, and consequently the release rate. The result indicated that P9, with half-sequence ionic complement, may serve as a hydrophobic compounds carrier. | RUAN LiPing ZHANG HangYu LUO HanLin ZHAO XiaoJun | 2009 | Science China Chemistry2009，52，5： | 4 |
| 16 | The combination of chidamide with the CHOEP regimen in previously untreated patients with peripheral T-cell lymphoma: a prospective, multicenter, single arm, phase 1b/2 study显示文摘Objective:To assess the efficacy and safety of the novel histone deacetylase inhibitor,chidamide,in combination with cyclophosphamide,doxorubicin,vincristine,etoposide,and prednisone(Chi-CHOEP)for untreated peripheral T-cell lymphoma(PTCL).Methods:A prospective,multicenter,single arm,phase 1 b/2 study was conducted.A total of 128 patients with untreated PTCL(18–70 years of age)were enrolled between March 2016 and November 2019,and treated with up to 6 cycles with the Chi-CHOEP regimen.In the phase 1 b study,3 dose levels of chidamide were evaluated and the primary endpoint was determination of the maximumtolerated dose and recommended phase 2 dose(RP2 D).The primary endpoint of the phase 2 study was 2-year progression-free survival(PFS).Results:Fifteen patients were enrolled in the phase 1 b study and the RP2 D for chidamide was determined to be 20 mg,twice a week.A total of 113 patients were treated at the RP2 D in the phase 2 study,and the overall response rate was 60.2%,with a complete response rate of 40.7%.At a median follow-up of 36 months,the median PFS was 10.7 months,with 1-,2-,and 3-year PFS rates of 49.9%,38.0%,and 32.8%,respectively.The Chi-CHOEP regimen was well-tolerated,with grade 3/4 neutropenia occurring in approximately two-thirds of the patients.No unexpected adverse events(AEs)were reported and the observed AEs were manageable.Conclusions:This large cohort phase 1 b/2 study showed that Chi-CHOEP was well-tolerated with modest efficacy in previously untreated PTCL patients. | Wei Zhang Liping Su Lihong Liu Yuhuan Gao Quanshun Wang Hang Su Yuhuan Song Huilai Zhang Jing Shen Hongmei Jing Shuye Wang Xinan Cen Hui Liu Aichun Liu Zengjun Li Jianmin Luo Jianxia He Jingwen Wang O.A.O’Connor Daobin Zhou | 2021 | Cancer Biology & Medicine2021，18，3： | 4 |
| 17 | Loss of Wip1 aggravates brain injury after ischaemia/reperfusion by overactivating microglia显示文摘Background and purpose The inflammatory response mediated by microglia/macrophages is closely related to cerebral ischaemia/reperfusion injury.Wild-type p53-induced protein phosphatase 1(Wip1),a serine/threonine phosphatase,is expressed in various tissues.A growing number of reports have suggested that Wip1 is a negative regulator of inflammation in peripheral tissue;however,its role in the central nervous system(CNS)remains unclear.This study aimed to clarify whether Wip1 can inhibit CNS inflammation by regulating microglia/macrophage functions after ischaemic injury.Methods A model of middle cerebral artery occlusion and reperfusion was established in mice.CNS inflammation was simulated by lipopolysaccharide treatment of primary microglia.Laser speckle imaging was used to monitor regional cerebral blood flow.Behavioural outcomes were assessed with a TreadScan gait analysis system.TTC staining was used to evaluate the infarct volume,and western blotting and immunofluorescence staining were applied to detect the phenotypical transformation of microglia.ELISA was performed to detect the levels of inflammatory factors.Results Wip1 expression was increased after ischaemia/reperfusion.Wip1-knockout(KO)mice displayed more severe brain injury than wild-type mice,as indicated by aggravated motor dysfunction,greater brain infarct volumes and higher expression of inflammatory cytokines(interleukin-6 and tumour necrosis factor alpha)in the brain.We also found that Wip1 depletion increased microglial/macrophage activation in both in vitro and in vivo models,which all showed activation of microglia/macrophages.Lentivirus-Ppm1d reversed the injury induced by Wip1-KO.Conclusions Our results suggest that Wip1 may inhibit neuroinflammation by inhibiting microglial/macrophage activation after brain ischaemia/reperfusion injury. | Feng Yan Xiang Cheng Ming Zhao Shenghui Gong Ying Han Liping Ding Di Wu Yumin Luo Wei Zuo Lingling Zhu Ming Fan Xunming Ji | 2021 | Stroke & Vascular Neurology2021，6，3： | 4 |
| 18 | δ^(13)C and water-use efficiency indicated by δ^(13)C of different plant functional groups on Changbai Mountains, Northeast China显示文摘Leaf δ13C of different plant functional groups (trees, shrubs and forbs; evergreen and deciduous; annual, biennial and perennial) were examined on the Changbai Mountains, China. Life form has a significant influence on plant δ13C, suggesting that leaf δ13C is also ideal for distinguishing functional groups species in temperate and frigid zones with high humidity. Additionally, the difference of water-use efficiency (WUE) is significant among different plant functional groups. δ13C and WUE are in the following order of forbs < shrubs < trees. Within shrubs, δ13C and WUE are higher for evergreen shrubs than deciduous shrubs. Differences in δ13C and WUE are significant among different lifespan herbs, and δ13C and WUE follow the order of annual herbs > biennial herbs > perennial herbs, not in accordance with the pattern obtained by previous studies in deserts, suggesting that the ranking of δ13C and WUE among annual, biennial and perennial herbs may be dependent on local water availability. | TAN WenBing WANG GuoAn HAN JiaMao LIU Min ZHOU LiPing LUO Ting CAO ZiYu CHENG ShuZhi | 2009 | Chinese Science Bulletin2009，54，10： | 4 |
| 19 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 20 | An in situ ATR-FTIR study of mixed collectors BHA/DDA adsorption in ilmenite-titanaugite flotation system显示文摘This paper researched the enhanced flotation separation performance of ilmenite and titanaugite using the mixed collector benzhydroxamic acid/dodecylamine(BHA/DDA).The interface assembly mechanism was mainly investigated through in situ attenuated total reflectance Fourier transform infrared(ATRFTIR)spectroscopy combined with the two-dimensional correlation spectroscopy(2D-COS)and X-ray photoelectron spectroscopy(XPS).It has been found that BHA/DDA mixed collectors successfully separate ilmenite from titanaugite at a molar ratio of 8:1.Zeta potential experiments suggested that,in the presence of mixed collector system,the BHA-DDA complex adsorbed on the ilmenite surface via the chemically adsorbed BHA and the electrostatically adsorbed DDA,however,the complex adsorbed on the surface of titanaugite unstably.According to in situ ATR-FTIR combined with 2D-COS and XPS results,the interface assembly mechanism of BHA/DDA is summarized as:the function group of BHA molecules first binds to the metal sites on minerals to form bidentate ligand,then DDA co-adsorbed with BHA on the surface of minerals through hydrogen bonding.DDA may change the adsorption modes of some BHA on the ilmenite surface from four-membered ring to five-membered ring,while the modes on the titanaugite surface is true opposite.Finally,recommended adsorption configurations of the BHA/DDA complex on the two mineral surfaces are proposed. | Liping Luo Houqin Wu Longhua Xu Jinping Meng Jiahui Lu Huan Zhou Xiaomei Huo Lingyun Huang | 2021 | International Journal of Mining Science and Technology2021，31，4： | 4 |
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