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379篇 您的检索式:作者名="Lei YUN"
    题名 作者 年代 出处 被引量
1Production of bioactive ginsenoside compound K in metabolically engineered yeast显示文摘Xing Yan Yun Fan Wei Wei Pingping Wang Qunfang Liu Yongjun Wei Lei Zhang Guoping Zhao Jianmin Yue Zhihua Zhou 2014Cell Research2014,24,6:81
2Persistence and clearance of viral RNA in 2019 novel coronavirus disease rehabilitation patients显示文摘Background:A patient’s infectivity is determined by the presence of the virus in different body fluids,secretions,and excreta.The persistence and clearance of viral RNA from different specimens of patients with 2019 novel coronavirus disease(COVID-19)remain unclear.This study analyzed the clearance time and factors influencing 2019 novel coronavirus(2019-nCoV)RNA in different samples from patients with COVID-19,providing further evidence to improve the management of patients during convalescence.Methods:The clinical data and laboratory test results of convalescent patients with COVID-19 who were admitted to from January 20,2020 to February 10,2020 were collected retrospectively.The reverse transcription polymerase chain reaction(RT-PCR)results for patients’oropharyngeal swab,stool,urine,and serum samples were collected and analyzed.Convalescent patients refer to recovered non-febrile patients without respiratory symptoms who had two successive(minimum 24 h sampling interval)negative RT-PCR results for viral RNA from oropharyngeal swabs.The effects of cluster of differentiation 4(CD4)+T lymphocytes,inflammatory indicators,and glucocorticoid treatment on viral nucleic acid clearance were analyzed.Results:In the 292 confirmed cases,66 patients recovered after treatment and were included in our study.In total,28(42.4%)women and 38 men(57.6%)with a median age of 44.0(34.0-62.0)years were analyzed.After in-hospital treatment,patients’inflammatory indicators decreased with improved clinical condition.The median time from the onset of symptoms to first negative RT-PCR results for oropharyngeal swabs in convalescent patients was 9.5(6.0-11.0)days.By February 10,2020,11 convalescent patients(16.7%)still tested positive for viral RNA from stool specimens and the other 55 patients’stool specimens were negative for 2019-nCoV following a median duration of 11.0(9.0-16.0)days after symptom onset.Among these 55 patients,43 had a longer duration until stool specimens were negative for viral RNA than for throat swabs,with a median delay of 2.0(1.0-4.0)days.Results for only four(6.9%)urine samples were positive for viral nucleic acid out of 58 cases;viral RNA was still present in three patients’urine specimens after throat swabs were negative.Using a multiple linear regression model(F=2.669,P=0.044,and adjusted R2=0.122),the analysis showed that the CD4+T lymphocyte count may help predict the duration of viral RNA detection in patients’stools(t=-2.699,P=0.010).The duration of viral RNA detection from oropharyngeal swabs and fecal samples in the glucocorticoid treatment group was longer than that in the non-glucocorticoid treatment group(15 days vs.8.0 days,respectively;t=2.550,P=0.013)and the duration of viral RNA detection in fecal samples in the glucocorticoid treatment group was longer than that in the non-glucocorticoid treatment group(20 days vs.11 days,respectively;t=4.631,P<0.001).There was no statistically significant difference in inflammatory indicators between patients with positive fecal viral RNA test results and those with negative results(P>0.05).Conclusions:In brief,as the clearance of viral RNA in patients’stools was delayed compared to that in oropharyngeal swabs,it is important to identify viral RNA in feces during convalescence.Because of the delayed clearance of viral RNA in the glucocorticoid treatment group,glucocorticoids are not recommended in the treatment of COVID-19,especially for mild disease.The duration of RNA detection may relate to host cell immunity.Yun Ling Shui-Bao Xu Yi-Xiao Lin Di Tian Zhao-Qin Zhu Fa-Hui Dai Fan Wu Zhi-Gang Song Wei Huang Jun Chen Bi-Jie Hu Sheng Wang En-Qiang Mao Lei Zhu Wen-Hong Zhang Hong-Zhou Lu 2020Chinese Medical Journal2020,,9:58
3Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS.Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu 2017Science China(Life Sciences)2017,60,4:39
42000–2020年中国氮氧化物排放清单及排放趋势(英文)显示文摘研究目的:建立2000–2020年中国氮氧化物排放清单,了解中国主要行业和省份氮氧化物的排放情况,为评估氮氧化物的环境影响和制定相关减排政策提供依据。创新要点:分析了中国主要省份产业结构对氮氧化物排放量的影响;根据不同情景分析,预测2020年中国氮氧化物的排放量。研究方法:1.基于自底向上法,根据不同类型化石燃料的氮氧化物排放因子,结合化石燃料消耗量,建立中国2000–2010年氮氧化物排放清单;2.基于IPAT方程,并以中国2000–2010年的国内生产总值增长数据和氮氧化物排放量为依据,分三种情景,分析2011–2020年中国能源消耗和氮氧化物排放趋势。重要结论:2010年中国氮氧化物的排放量约是2000年的两倍;自2009年起,中国氮氧化物总排放量超过了二氧化硫总排放量;主要由于产业结构和地区生产总值的不同,中国东部和西部氮氧化物排放量有明显差异;制造业、电力行业和交通运输业是中国氮氧化物的主要排放源,其中交通运输业氮氧化物排放量呈现逐年增长趋势;预计2020年中国氮氧化物排放量为19.7 Mt。Yun SHI Yin-feng XIA Bi-hong LU Nan LIU Lei ZHANG Su-jing LI Wei LI 2014Journal of Zhejiang University-Science A(Applied Physics & Engineering)2014,15,6:34
5Chemical composition and source apportionment of PM10 and PM2.5 in different functional areas of Lanzhou,China显示文摘To elucidate the air pollution characteristics of northern China, airborne PM_10(atmospheric dynamic equivalent diameter ≤ 10 μm) and PM_(2.5)(atmospheric dynamic equivalent diameter ≤ 2.5 μm) were sampled in three different functional areas(Yuzhong County,Xigu District and Chengguan District) of Lanzhou, and their chemical composition(elements, ions, carbonaceous species) was analyzed. The results demonstrated that the highest seasonal mean concentrations of PM_10(369.48 μg/m^3) and PM_(2.5)(295.42 μg/m^3) were detected in Xigu District in the winter, the lowest concentration of PM_(2.5)(53.15 μg/m^3) was observed in Yuzhong District in the fall and PM_10(89.60 μg/m^3) in Xigu District in the fall.The overall average OC/EC(organic carbon/elemental carbon) value was close to the representative OC/EC ratio for coal consumption, implying that the pollution of Lanzhou could be attributed to the burning of coal. The content of SNA(the sum of sulfate, nitrate,ammonium, SNA) in PM_(2.5)in Yuzhong County was generally lower than that at other sites in all seasons. The content of SNA in PM_(2.5)and PM_10 in Yuzhong County was generally lower than that at other sites in all seasons(0.24–0.38), indicating that the conversion ratios from precursors to secondary aerosols in the low concentration area was slower than in the area with high and intense pollutants. Six primary particulate matter sources were chosen based on positive matrix factorization(PMF) analysis, and emissions from dust, secondary aerosols, and coal burning were identified to be the primary sources responsible for the particle pollution in Lanzhou.Xionghui Qiu Lei Duan Jian Gao Shulan Wang Fahe Chai Jun Hu Jingqiao Zhang Yaru Yun 2016Journal of Environmental Sciences2016,28,2:33
6Trends in Drug Resistance of Acinetobacter baumannii over a 10-year Period: Nationwide Data from the China Surveillance of Antimicrobial Resistance Program显示文摘Lei Gao Yuan Lyu Yun Li 2017Chinese Medical Journal2017,,6:22
7Effects of precipitation and landuse on runoff during the past 50 years in a typical watershed in the Loess Plateau, China显示文摘In the past century, great progress has been made worldwide in our understanding of forest-water relationship. The successful forestation programs implemented in China-which have improved the ecological environmental conditions-have gained the attention of many researchers and highlighted the relationship between forestation and water yields. The arid and semi-arid Loess Plateau has received attention from water engineers and eco-hydrological researchers in China because of a shortage in water resources. We selected one of the oldest stations conducting soil and water conservation experiments, the Xifeng soil and water conservation station, and chose the Nanxiaohe catchment and its paired catchments (Yangjiagou catchment and Dongzhuanggou catchment) as our research areas. Trends in precipitation, air temperature, streamflow over the past 50 years, and the effect of changing land use on streamflow were analyzed. The Mann-Kendall test showed that precipitation had a negative trend (downward trend), whereas air temperature showed a positive trend (upward trend) from the past to present in the Nanxiaohe catchment. However, the trends seen in precipitation, air temperature did not contain any 'jumping points.' The paired catchment approach is used to detect the effects of land cover change on hydrology in the Yangjiagou and the contrast catchment, i.e., Dongzhuanggou catchment in our study. The results showed a large change in land use in the Yangjiagou catchment from 1954 to 2008. An increase in forested land (from 0% to 40.08% from 1954 to 2008) and a reduction of bare land (from 51.26% to 5.50% from 1954 to 2008) accounted for a large part of the change in land use. However, the land use changed little in the contrast catchment. The comparison of streamflow in the paired catchments showed that forestation reduced streamflow by 49.63% (or 6.5 mm) each year.Huaxing BI Bin LIU Jie WU Lei YUN Zhihan CHEN Zhewei CUI 2009International Journal of Sediment Research2009,24,3:21
8Management of cytokine release syndrome related to CAR-T cell therapy显示文摘Chimeric antigen receptor T (CAR-T) cell therapy is a novel cellular immunotherapy that is widely used to treat hematological malignancies, including acute leukemia, lymphoma, and multiple myeloma. Despite its remarkable clinical effects, this therapy has side effects that cannot be underestimated. Cytokine release syndrome (CRS) is one of the most clinically important and potentially life-threatening toxicities. This syndrome is a systemic immune storm that involves the mass cytokines releasing by activated immune cells. This phenomenon causes multisystem damages and sometimes even death. In this study, we reported the management of a patient with recurrent and refractory multiple myeloma and three patients with acute lymphocytic leukemia who suffered CRS during CAR-T treatment. The early application of tocilizumab, an anti-IL-6 receptor antibody, according to toxicity grading and clinical manifestation is recommended especially for patients who suffer continuous hyperpyrexia, hypotensive shock, acute respiratory failure, and whose CRS toxicities deteriorated rapidly. Moreover, low doses of dexamethasone (5-10 mg/day) were used for refractory CRS not responding to tocilizumab. The effective management of the toxicities associated with CRS will bring additional survival opportunities and improve the quality of life for patients with cancer.Hongli Chen Fangxia Wang Pengyu Zhang Yilin Zhang Yinxia Chen Xiaohu Fan Xingmei Cao Jie Liu Yun Yang Baiyan Wang Bo Lei Liufang Gu Ju Bai Lili Wei Ruili Zhang Qiuchuan Zhuang Wanggang Zhang Wanhong Zhao Aili He 2019Frontiers of Medicine2019,13,5:21
9Comparison of glycemic variability and glycated hemoglobin as risk factors of coronary artery disease in patients with undiagnosed diabetes显示文摘背景一个冠的动脉疾病( CAD )风险因素是著名的长期的多糖症的角色,并且 glycemic 可变性仍然是这研究的 debate.The 目的一件事是调查承认 glycemic 的协会有在有 undiagnosed 糖尿病 mellitus ( DM )的病人的 CAD 的存在和严厉的旅行和血红素 A1c ( HbA1c )我们学习了的 .Methods 286 最新诊断了 DM 没有优先的 revascularization 的病人为怀疑的 ischaemic ch 经历冠的MI Shu-hua SU Gong LI Zhao YANG Hong-xia ZHENG Hong TAO Hong ZHOU Yun TIAN Lei 2012Chinese Medical Journal2012,,1:18
10Berbamine, a novel nuclear factor κB inhibitor, inhibits growth and induces apoptosis in human myeloma cells显示文摘Yun LIANG Rong-zhen XU Lei ZHANG Xiao-ying ZHAO 2009Acta Pharmacologica Sinica2009,30,12:17
11The Prevalence of Nonalcoholic Fatty Liver Disease and its Association with Lifestyle/dietary Habits among University Faculty and Staff in Chengdu显示文摘Objective To investigate the prevalence of nonalcoholic fatty liver disease(NAFLD) in different university categories and its association with lifestyle/dietary habits.Methods A cross-sectional study was carried out on 9 378 faculty members and staff who participated in an annual health checkup at three universities selected by random cluster sampling.Demographic,anthropometric,biochemical indices and abdominal ultrasound measurements were collected.A nested case-control study was conducted with 200 NAFLD cases and 200 controls matched by gender,age(±3 years),and university.Results The overall prevalence of NAFLD was 10.3%(13.7% in males and 6.8% in females).The prevalence was significantly higher in the science and engineering university(22.1%) than in the comprehensive universities with(6.4%) and without(10.9%) medical colleges.Obesity/overweight,hyperlipidemia,diabetes mellitus,and family history of NAFLD were independently associated with higher risk of NAFLD,as were frequent consumption of desserts and salty/spicy foods.Using nutritional supplements was a protective factor against NAFLD.Intake of coarse cereals,potatoes,vegetables,fruits,and milk was significantly lower,and intake of red meat,viscera,candies and pastries,cooking oil,and total energy was significantly higher in participants with NAFLD than in controls.Conclusion Science and engineering university faculty and staff are key targets for NAFLD prevention.NAFLD is closely associated with age,gender,university type,metabolic diseases,and lifestyle/dietary habits.SHI Lei LIU Zhong Wu LI Yun GONG Cai ZHANG Huan SONG Li Juan HUANG Cheng Yu LI Ming 2012Biomedical and Environmental Sciences2012,25,4:16
12NUMERICAL PREDICTION OF FATIGUE DAMAGE IN STEEL CATENARY RISER DUE TO VORTEX-INDUCED VIBRATION显示文摘For studying the characteristics of Steel Catenary Riser (SCR), a simplified pinned-pinned cable model of vibration is established. The natural frequencies, the normalized mode shapes and mode curvatures of the SCR are calculated. The fatigue damage of the SCR can be obtained by applying the modal superposition method combined with the parameters of S - N curve. For analyzing the relation between the current velocity and the SCR's fatigue damage induced by the vortex-induced vibration, ten different current states are evaluated. Then, some useful conclusions are drawn, especially an important phenomenon is revealed that the maximum fatigue damage in the riser usually occurs near the area of the boundary ends.GAO Yun ZONG Zhi SUN Lei 2011Journal of Hydrodynamics2011,23,2:15
13Self-microemulsifying drug delivery system for improving the bioavailability of huperzine A by lymphatic uptake显示文摘Huperzine A(Hup-A) is a poorly water-soluble drug with low oral bioavailability. A selfmicroemulsifying drug delivery system(SMEDDS) was used to enhance the oral bioavailability and lymphatic uptake and transport of Hup-A. A single-pass intestinal perfusion(SPIP) technique and a chylomicron flow-blocking approach were used to study its intestinal absorption, mesenteric lymph node distribution and intestinal lymphatic uptake. The value of the area under the plasma concentration–time curve(AUC) of Hup-A SMEDDS was significantly higher than that of a Hup-A suspension(P <0.01).The absorption rate constant(K_a) and the apparent permeability coefficient(P_(app)) for Hup-A in different parts of the intestine suggested a passive transport mechanism, and the values of K_a and P_(app) of Hup-A SMEDDS in the ileum were much higher than those in other intestinal segments. The determination of Hup-A concentration in mesenteric lymph nodes can be used to explain the intestinal lymphatic absorption of Hup-A SMEDDS. For Hup-A SMEDDS, the values of AUC and maximum plasma concentration(C_(max)) of the blocking model were significantly lower than those of the control model(P<0.05). The proportion of lymphatic transport of Hup-A SMEDDS and Hup-A suspension were about 40% and 5%,respectively, suggesting that SMEDDS can significantly improve the intestinal lymphatic uptake and transport of Hup-A.Fang Li Rongfeng Hu Bin Wang Yun Gui Gang Cheng Song Gao Lei Ye Jihui Tang 2017Acta Pharmaceutica Sinica B2017,7,3:15
14Performance analysis and comparison of PoW,PoS and DAG based blockchains显示文摘In the blockchain,the consensus mechanism plays a key role in maintaining the security and legitimation of contents recorded in the blocks.Various blockchain consensus mechanisms have been proposed.However,there is no technical analysis and comparison as a guideline to determine which type of consensus mechanism should be adopted in a specific scenario/application.To this end,this work investigates three mainstream consensus mechanisms in the blockchain,namely,Proof of Work(PoW),Proof of Stake(PoS),and Direct Acyclic Graph(DAG),and identifies their performances in terms of the average time to generate a new block,the confirmation delay,the Transaction Per Second(TPS)and the confirmation failure probability.The results show that the consensus process is affected by both network resource(computation power/coin age,buffer size)and network load conditions.In addition,it shows that PoW and PoS are more sensitive to the change of network resource while DAG is more sensitive to network load conditions.Bin Cao Zhenghui Zhang Daquan Feng Shengli Zhang Lei Zhang Mugen Peng Yun Li 2020Digital Communications and Networks2020,6,4:15
15A novel partner of Scalloped regulates Hippo signaling via antagonizing Scalloped-Yorkie activity显示文摘由调整 transcriptional 激活剂 Yorkie (Yki ) 的活动的河马(Hpo ) 小径控制织物生长和机关尺寸,它在原子核与抄写因素 Scalloped (Sd ) 联系下游地支持指向基因表示。这里,我们识别新奇蛋白质 Sd-Binding-Protein (SdBP )/Tgi,它直接通过它的 TDU 域为绑定与 Yki 竞争到 Sd 并且禁止 Sd-Yki transcriptional 活动。我们也发现 SdBP 经由它的 PPXY 主题与 Yki WW 领域通过协会在原子核保留 Yki。一起,我们作为 Hpo 小径的一个新奇部件识别 SdBP,否定地调整 Sd-Yki 的 transcriptional 活动限制织物生长。Tong Guo Yi Lu Peixue Li Meng-Xin Yin Dekang Lv Wenjing Zhang Huizhen Wang Zhaocai Zhou Hongbin Ji Yun Zhao Lei Zhang 2013Cell Research2013,23,10:13
16A hybrid invisibility cloak based on integration of transparent metasurfaces and zero-index materials显示文摘The invisibility cloak,a long-standing fantastic dream for humans,has become more tangible with the development of metamaterials.Recently,metasurface-based invisibility cloaks have been proposed and realized with significantly reduced thickness and complexity of the cloaking shell.However,the previous scheme is based on reflection-type metasurfaces and is thus limited to reflection geometry.In this work,by integrating the wavefront tailoring functionality of transparent metasurfaces and the wave tunneling functionality of zero-index materials,we have realized a unique type of hybrid invisibility cloak that functions in transmission geometry.The principle is general and applicable to arbitrary shapes.For experimental demonstration,we constructed a rhombic double-layer cloaking shell composed of a highly transparent metasurface and a double-zero medium consisting of dielectric photonic crystals with Dirac cone dispersions.The cloaking effect is verified by both full-wave simulations and microwave experimental results.The principle also reveals exciting possibilities for realizing skin-thick ultrathin cloaking shells in transmission geometry,which can eliminate the need for spatially varying extreme parameters.Our work paves a path for novel optical and electromagnetic devices based on the integration of metasurfaces and metamaterials.Hongchen Chu Qi Li Bingbing Liu Jie Luo Shulin Sun Zhi Hong Hang Lei Zhou Yun Lai 2018Light(Science & Applications)2018,7,1:13
17Berbamine exhibits potent antitumor effects on imatinib-resistant CML cells in vitro and in vivo显示文摘Yan-lin WEI Lei XU Yun LIANG Xiao-hua XU, Xiao-ying ZHA 2009Acta Pharmacologica Sinica2009,30,4:13
18Phosphorus oxoanion-intercalated layered double hydroxides for high-performance oxygen evolution显示文摘合理设计和为氧进化反应(OER ) 的有效、划算的电极的控制制造为探讨崭新的精力危机是批评的。有特定的夹层阴离子的镍铁分层的双氢氧化物(NiFe-LDHs )( 即磷酸盐,亚磷酸盐,和次磷酸盐) 被一个一起沉淀方法制作并且作为氧进化 electrocatalysts 调查了。磷 oxoanion 的置闰在一个碱的答案提高了 OER 活动;最佳的表演(即, 215 mV, 37.7 mV/dec 的一个小 Tafel 斜坡,和稳定的电气化学的行为的一个低发作潜力) 与设置次磷酸盐的 NiFe-LDH 催化剂被完成,在传统的设置碳酸盐的 NiFe-LDH 上的示威戏剧的改进以活动和耐久性。这提高的表演被归因于在设置的磷的 oxoanions 和分享边的瞬间 6 之间的相互作用(M = Ni, Fe ) 层,它修改表面 Ni 地点的电子结构。这个概念应该为更有效的基于 LDH 的氧进化 electrocatalysts 的设计是鼓舞人心的。Ma Luo Zhao Cai Cheng Wang Yongmin Bi Li Qian Yongchao Hao Li Li Yun Kuang Yaping Li Xiaodong Lei Ziyang HUO Wen Liu Hailiang Wang Xiaoming Sun Xue Duan 2017Nano Research2017,10,5:12
19Transduction of the Hedgehog signal through the dimerization of Fused and the nuclear translocation of Cubitus interruptus显示文摘分泌蛋白质的刺猬(Hh ) 家庭为在脊椎动物和无脊椎动物的开发是必要的。作为主要 morphogens 之一在后生动物的开发期间,分级的 Hh 信号通过它的细胞质的尾巴的微分 phosphorylation 是越过由 Smoothened (Smo ) 的血浆膜的 transduced,导致小径激活和目标基因的微分表达式。然而,怎么 Smo transduces 经由建筑群糟糕仍然是的 Costal2 (Cos2 )/Fused (Fu ) 的分级的 Hh 信号理解。这里我们在场对由翻译 Smo phosphorylation 信息到 Fu dimerization 和肘脉 interruptus (Ci ) 的一个 Hh 坡度的房间反应的一个模型原子本地化信息。我们的调查结果建议 Smo 的 phosphorylated C 终点招募 Cos2/Fu 建筑群到通过在 Smo 和 Cos2 之间的相互作用的膜,它进一步导致 Fu dimerization。使聚合成二聚物的 Fu 是 phosphorylated 和 transduces 由 phosphorylating Cos2 和 Fu (苏(fu )) 的 Suppressor 的 Hh 信号。我们进一步证明这个过程支持全身的 Ci (Ci155 ) 和 Cos2 或苏(fu ) 的分离,并且导致 Ci155 的 translocation 进原子核,激活目标基因的表示。Yanyan Zhang Feifei Mao Yi Lu Wenqing Wu Lei Zhang Yun Zhao 2011Cell Research2011,21,10:12
20Combined treatment with erythropoietin and granulocyte colony-stimulating factor enhances neovascularization and improves cardiac function after myocardial infarction显示文摘Xue Jingyi Du Guoqing Shi Jing Li Yue Masahiro Yasutake Liu Lei Li Jianqiang Kong Yihui Wang Shuxian Yun Fengxiang Li Weimin 2014Chinese Medical Journal2014,,9:12
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