|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A DELLA protein complex controls the arbuscular mycorrhizal symbiosis in plants显示文摘 | Nan Yu Dexian Luo Xiaowei Zhang Junzhong Liu Wanxiao Wang Yue Jin Wentao Dong Jiyun Liu Huan Liu Weibing Yang Longjun Zeng Qun Li Zuhua He Giles E D Oldroyd Ertao Wang | 2014 | Cell Research2014，24，1： | 9 |
| 2 | Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast显示文摘Tumor necrosis factor-related apop-tosis-inducing ligand (TRAIL) induces selectively apoptosis in various tumor cells and virus-infected cells, but rarely in normal cells. A chloroplast expres-sion vector, p64TRAIL, containing the cDNA coding for the soluble TRAIL (sTRAIL), was constructed with clpP-trnL-petB-chlL-rpl23-rpl2 as Chlamydomonas reinhardtii plastid homologous recombinant frag-ments and spectinomycin-resistant aadA gene as a select marker. The plasmid p64TRAIL was trans-ferred into the chloroplast genome of C. reinhardtii by the biolistic method. Three independently trans-formed lines were obtained by 100 mg/L spectino-mycin selection. PCR amplification, Southern blot analysis of the sTRAIL coding region DNA and culti-vation cells in the dark all showed that the exogenous DNA had been integrated into chloroplast genome of C. reinhardtii. Western blot analysis showed that human soluble TRAIL was expressed in C. reinhardtii chloroplast. The densitometric analysis of Western blot indicated that the expressed human sTRAIL protein in the chloroplasts of C. reinhardtii accounted for about 0.43%―0.67% of the total soluble proteins. These experimental results demonstrated the possi-bility of using transgenic chloroplasts of green alga as bioreactors for production of biopharmaceuticals. | YANG Zongqi LI yinü CHEN Feng LI Dong ZHANG Zhifang LIU Yanxin ZHENG Dexian WANG Yong SHEN Guifang | 2006 | Chinese Science Bulletin2006，51，14： | 4 |
| 3 | Atacamite and Nantokite in Kaerqueka Copper Deposit of Qimantag Area:Evidence for Cenozoic Climate Evolution of the Qaidam Basin显示文摘Using a mineralogy method to reflect climate evolution is a new approach to research Cenozoic environmental progression of the Qaidam Basin. In this paper, we present the chemical composition and crystallographic parameter of atacamite, nantokite, and tenorite in cryptocrystalline aggregates from the Kaerqueka copper deposit of Qimantag metallogenic belt by means of electron microprobe and in-situ X-ray diffraction analyses. Atacamite and nantokite occur in an intimate intergrowth as the secondary precipitation of chalcopyrite and bornite filling in the interstitial space of andradite in the Kaerqueka copper deposit, with an average composition of Cl: 12.38 wt.%, Cu: 63.76 wt.%, O: 21.46 wt.%. X-ray microdiffraction shows that the intergrowth contains nantokite with a cubic unit cell a=5.403(2) ? and atacamite with an orthorhombic unit cell a=6.030(3), b=6.883(2), c=9.114(1) ?. XRD quantitative calculation shows that the nanometric aggregate contains 36.07 wt.% tenorite, 18.41 wt.% atacamite, and 45.52 wt.% nantokite. The presence of nantokite and atacamite requires saline solutions for their formation and hyper-arid climate conditions for their preservation. Combined with the data of salt lakes and the pollen sequence of western China, we suggest that during the uplift of the Tibetan Plateau and the retreat of the Paratethys, saline water was forced to the surface through a basal fracture zone. In the hyper-arid climate of the Qaidam Basin, the recharge of groundwater by direct precipitation is negligible, and groundwater is derived from inflow from the salt lakes. Thus, atacamite is preserved. In addition, spertiniite in the edge and fractures of atacamite and nantokite may represent wetter climate after the formation of atacamite and nantokite. | Liwen Yi Xiangping Gu Anhuai Lu Xiangyu Li Dexian Zhang Zhiling Wang Jianping Liu Shuai Li Zhengxiang Shu Cui Yu Hongyan Zuo Can Shen | 2017 | Journal of Earth Science2017，28，3： | 3 |
| 4 | Influences of the Three Gorges Dam in China on Precipitation over Surrounding Regions显示文摘Impacts of the Three Gorges Dam(TGD) in China on the regional pattern and annual amount of precipitation around the Three Gorges Reservoir(TGR) are examined by comparing observations before and after the operation of TGD(1984–2003 and 2004–13). Empirical orthogonal function(EOF) analysis of the annual precipitation anomalies clearly indicates that the land-use change associated with the construction of TGD has not significantly changed the precipitation pattern. To investigate the impacts of TGD on the rainfall amount, we compare the relative variations of atmospheric variables related to precipitation formation in three spatial bands: over TGR, near TGR, and far from TGR. It is found that the differences in annual rainfall over TGD between the two periods before and after the operation of TGD are small, suggesting a weak impact of TGD on the rainfall amount. The TGD water level increased from 66 m before June 2003 to 175 m after 2010, and this may have slightly reduced precipitation on the local scale. | Yan LI Weican ZHOU Xianyan CHEN Dexian FANG Qianqian ZHANG | 2017 | Journal of Meteorological Research2017，31，4： | 1 |
| 5 | CXCL12/CXCR4 axis plays pivotal roles in the organ-specific metastasisof pancreatic adenocarcinoma: A clinical study显示文摘 | Weixia Zhong Weiwei Chen Dexian Zhang Jujie Sun Yuhui Li Jianbo Zhang Yongsheng Gao Wuyuan Zhou Sheng Li | 2012 | Experimental and Therapeutic Medicine2012，，3： | 1 |
| 6 | Removal of humic substances from soil DNA using aluminum sulfate 显示文摘 | DONG Dexian YANG An LI Haiming | 2006 | J Microb Meth2006，66，： | 1 |
| 7 | A kinetic study of the oxidation of arsenopyrite in acidic solutions: implications for the environment显示文摘 | Yu Yunmei Zhu Yongxuan A.E. Williams-Jones Gao Zhenmin Li Dexian | 2003 | Applied Geochemistry2003，，3： | 1 |
| 8 | The use of folate-PEG-grafted-hybranched-PEI nonviral vector for the inhibition of glioma growth in the rat显示文摘 | Bing Liang Ming-Liang He Chu-yan Chan Yang-chao Chen Xiang-Ping Li Yi Li Dexian Zheng Marie C. Lin Hsiang-Fu Kung Xin-Tao Shuai Ying Peng | 2009 | Biomaterials2009，，23： | 1 |
| 9 | Treatment of acid rose dye containing wastewater by ozonizing-biological aerated filter显示文摘 | Wang Xiaojun Gu Xiaoyang Li Dexian | 2007 | Dyes and Pigments2007，74，3： | 1 |
| 10 | Electrodeposition of platinum on poly(glutamic acid) modified glassy carbon electrode for non-enzymatic amperometric glucose detection显示文摘 | Xuechou Zhou Xinyu Zheng Rixin Lv Dexian Kong QingLu Li | 2013 | Electrochimica Acta2013，，： | 1 |
| 11 | Removal of humic substances from soil DNA using aluminum sulfate显示文摘 | DONG Dexian YANG An LI Haiming | 2006 | J Microb Meth2006，66，： | 1 |
| 12 | Effects of the polysaccharide from Pholiota nameko on human cytokine network in serum显示文摘 | Haiping Li Xiaojuan Liu Yang Li Yanjun Hua Dexian Zhi Guangchang Pang | 2011 | International Journal of Biological Macromolecules2011，，1： | 1 |
| 13 | Anticancer Activities of Citrus Peel Polymethoxyflavones Related to Angiogenesis and Others显示文摘 | Liwen Wang Jinhan Wang Lianying Fang Zuliang Zheng Dexian Zhi Suying Wang Shiming Li Chi-Tang Ho Hui Zhao Zongjin Li | 2014 | BioMed Research International2014，，： | 1 |
| 14 | The development of mine digitization information system and its application to the Lanping Pb-Zn mine显示文摘To compile the software package of the mine digitization information system in terms of the principle and methods of mathematics geology and geographic information system using computer languages such as VB and Matlab,this paper introduces the function composition and operating environment of this information system,and illustrates the function and effectiveness of the software package as exemplified by the Lanping Pb-Zn mine,Yunnan Province. | YAN Yongfeng QIN Dexian YU Yangxian LI Shilei JIA Fengqin WANG Xiaoli LIU Fangcheng | 2008 | Chinese Journal Of Geochemistry2008，27，3： | 1 |
| 15 | Cloning and identification of a novel binding protein of CD2 cytoplasmic domain显示文摘In order to understand why CD2 has a dual action of transduction of activation or apoptosis signals in T cells under different experimental conditions, we employed a yeast two-hybrid system to look for a binding protein of the cytoplasmic domain of CD2 which may be involved in this issue. A human T cell cDNA library was screened by a cDNA encoding the cytoplasmic domain of CD2 (Thr211-Gln336). The specificity of protein-protein interaction was verified by co-immunoprecipitation. The binding protein obtained, designated CD2cBP, was found to be homologous to v-fos transformation effector protein (Fte-1). As Fte-1 plays a role in cell transformation, growth, protein synthesis and protein-import into mitochondria, this result suggests that CD2cBP may be putatively involved in CD2-mediated signaling. | SHAO Yuan XIAO Sheng ZHAI Wenjing LI Dong LIU Yanxin LIU Shilian ZHENG Dexian | 2000 | Chinese Science Bulletin2000，45，24： | 1 |
| 16 | Treatment of acid rose dye containing wastewater by ozonizing-biological aerated filter显示文摘 | Wang Xiaojun Gu Xiaoyang Li Dexian | 2007 | Dyes and Pigments2007，74，： | 1 |
| 17 | Frequency of HLA-A*03 associates with HIV-1 infection in a Chinese cohort显示文摘During the early mid-1990s,a number of rural farmers across central China were employed to the unregulated plasmaselling-activity and many of them were infected by HIV-1.However,AIDS progression in the former blood donors(FBDs)is various.The aim of this study is to assess human leukocyte antigen(HLA)class I allele distribution in FBDs and evaluate its association with HIV-1 infection and disease progression.A total of 353 FBDs were enrolled in the cohort including 294 ART na ve HIV-1 seropositive and 59 HIV-1 seronegative age-matched subjects.The viral load and CD4/CD8 T cell counts were assessed in all subjects.Compared with HIV-seropositive group,the frequency of HLA-A*03 in control was significantly higher.After classifying the HLA-B alleles of the subjects according to the presence of Bw4/Bw6 serological epitopes,detrimental effect of HLA Bw6/Bw6 homozygosity was also confirmed in the HIV-seropositive subjects.This study provides novel evidence on HLA class I allele distribution and association of HLA-A*03 frequency with HIV-1 infection and viremia in the HIV-1 infected FBDs,which may throw light on intervention strategy for the HIV-1 infection and our understanding how host immunity and genetic background affect HIV infection and AIDS progression. | ZHANG WeiLun WANG Li HONG KunXue LIU YanXin SU Bing XU Cheng XU JianQin RUAN YuHua SHAO YiMin ZHENG DeXian | 2013 | Science China(Life Sciences)2013，56，11： | 0 |
| 18 | 5-Acetyl-6,7,8,4′-tetramethylnortangeretin induces apoptosis in multiple myeloma U266 cells显示文摘Multiple myeloma(MM)is one of the most common hematological malignancies and to date,it remains an incurable disease.In this study,we evaluated the inhibitory effect of 5-acetyloxy-6,7,8,4′-tetramethoxyflavone(5-ATMF),a compound from aged citrus peel extracts,on the MM U266 cell line.We found that the compound inhibited cell growth and induced cell apoptosis in multiple apoptotic assays.The apoptotic proteins caspase-3,caspase-9,and PARP(poly ADP-ribose polymerase)were cleaved when cells were treated with 5-ATMF.Along with the apoptosis process,the anti-apoptotic protein,Bcl-2(B-cell lymphoma-2),was significantly downregulated and the pro-apoptotic protein,Bax(Bcl-2 associated X protein),upregulated along with the release of cytochrome C(Cyt c)and the reduction of mitochondrial membrane potential(MMP).Notably,we found that the phosphorylation of Bad(Bcl-2/Bcl-XL-associated death promoter)was decreased but Bad remained unchanged in this process.On adding 5-ATMF to U266 cells,we observed that 5-ATMF repressed the phosphorylation of Akt(PKB Protein Kinase B PKB),thereby increasing the release of Cyt c and inhibiting the phosphorylation of Bad.These effects were enhanced by Ly294002,an inhibitor of Akt.These results suggested that 5-ATMF exerts a pro-apoptotic effect on U266 cells,possibly associated with the mitochondrial apoptotic pathway induced by the PI3K/Akt/Bad pathway. | Dexian Zhi Su Liu Li Lin Liwen Wang Jinhan Wang Jing Ma Suying Wang Hui Zhao Chi-Tang Ho Yafei Wang Qiang Liu | 2014 | Food Science and Human Wellness2014，3，3： | 0 |
| 19 | Evolution of the Mid-tropospheric Vortex during the Formation of Super Typhoon Megi(2010)显示文摘As a follow-up of a previously published article on the contribution of tropical waves,this study explores the evolution of the mid-tropospheric mesoscale cyclonic vortex(MV)during the formation of Typhoon Megi(2010)with a successful cloud-resolving simulation.It is found that the formation and intensification of the MV were related to the deep convection and subsequent stratiform precipitation,while the weakening of the MV was related to the shallow convection.Both the upward transport of vorticity related to the deep convection and the horizontal convergence associated with the stratiform precipitation contributed to the formation and intensification of the MV.Even though the latter was dominant,the former could not be ignored,especially in the early stage of the MV.The MV played dual roles in the formation of Megi.On the one hand,the formation and intensification of MV were primarily associated with the stratiform precipitation,which induced the low-level divergence inhibiting the spin-up of the near-surface cyclonic circulation.On the other hand,the coupled low-level cold core under the MV benefited the accumulation of the convective available potential energy(CAPE),which was favorable for the convective activity.A sensitivity experiment with the evaporative cooling turned off indicated that the development of the MV retarded the genesis process of Megi. | Li ZHUO Dexian FANG Zheng WU Juan FANG | 2020 | Advances in Atmospheric Sciences2020，37，7： | 0 |
| 20 | Effects of Solanum lycopersicum Rootstock Grafting on Tomato Resistance and Quality显示文摘[Objectives] This experiment was carried out to explore the effects of Solanum lycopersicum rootstock grafting on tomato resistance and quality. [Methods]6 different S. lycopersicum rootstocks were used to graft ‘Guihong 2' tomato. The grafting affinity was better and the resistance to bacterial wilt was higher.[Results]S. lycopersicum rootstock grafting may reduce the tomato,but it can improve the taste and endogenous quality of tomato. Besides,soluble solids,soluble sugars,Vc content,and sugar-acid ratio were all significantly higher than that of the control group. [Conclusions] S. lycopersicum rootstock y11,53,and 177 grafting can increase Vc,soluble solids,and sugar-acid ratio of tomato fruit,so they are optimal rootstocks for tomato grafting. | Guiyun GAN Kouhua YU Yaqin JIANG Yongcheng MO Wenjia LI Yongguan WU Yan LI Dexian KANG Yikui WANG | 2018 | Agricultural Biotechnology2018，7，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员