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| 1 | 2019新型冠状病毒基因组特征和流行病学:病毒起源和受体结合的意义显示文摘研究者对来自9例新型冠状病毒肺炎住院患者的支气管肺泡灌洗液样本和培养的分离株进行了下一代测序。从这些个体中获得了严重急性呼吸综合征-冠状病毒2(severe acute respiratory syndrome-coronavirus 2,SARS-CoV-2)的完整和部分基因组序列。利用Sanger测序连接病毒重叠群以获得全长基因组,cDNA末端快速扩增确定终端区。对这些SARSCoV-2基因组和其他冠状病毒基因组进行了系统进化分析,以确定该病毒的进化史并有助于推断其可能的起源。 | 刘青(译) 刘莉(审校) Lu R Zhao X Li J Niu P Yang B Wu H Wang W Song H Huang B Zhu N Bi Y Ma X Zhan F Wang L Hu T Zhou H Hu Z Zhou W Zhao L Chen J Meng Y Wang J Lin Y Yuan J Xie Z Ma J Liu WJ Wang D Xu W Holmes EC Gao GF Wu G Chen W Shi W Tan W | 2020 | 中华高血压杂志2020，28，3： | 516 |
| 2 | A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein显示文摘最新新兴的中东呼吸症候群 coronavirus (MERS-CoV ) 能在人引起严重、致命的急性呼吸疾病。尽管有全球努力,潜力为一联系在未来流行不能被排除。有效相对的措施的发展是迫切的。MERS-CoV-specific 抗病毒的药或疫苗是还没可得到的。使用 MERS-CoV (MERS-RBD ) 的尖铁受体绑定领域使老鼠免疫,我们识别了二抵销 monoclonal 抗体(mAbs ) 4C2 和 2E6。两 mAbs potently 与高功效在 vitro 绑在 MERS-RBD 和块病毒入口。我们进一步由使在 Fab 碎片和 RBD 之间的建筑群结晶调查了他们中立化的机制,并且解决了 4C2 Fab/MERS-RBD 建筑群的结构。结构证明 4C2 认出部分重叠的 epitope 在 MERS-RBD 的受体绑定脚印,从而由位的阻碍者和接口残余竞争防碍病毒 / 受体相互作用。2E6 也堵住受体绑定,并且为绑定与 4C2 竞争到 MERS-RBD。基于结构,我们进一步由保存仅仅 paratope 残余并且从人的免疫球蛋白与对应物代替留下的氨基酸使 4C2 人性化。人性化的 4C2 (4C2h ) 抗体支撑了类似的抵销的活动和生物化学的特征到父母老鼠抗体。最后,我们证明 4C2h 能显著地在感染 MERS-CoV 的 Ad5-hCD26-transduced 鼠标的肺消除病毒 titers,因此在临床的设置为预防和治疗代表一个有希望的代理人。 | Yan Li YuhuaWan Peipei Liu Jincun Zhao Guangwen Lu Jianxun Qi Qihui Wang Xuancheng LU Ying Wu Weniun Liu Buchang Zhang Kwok-Yung Yuen Stanley Perlman George F Gao Jinghua Yan | 2015 | Cell Research2015，25，11： | 14 |
| 3 | Concurrent alterations of O-GlcNAcylation and phosphorylation of tau in mouse brains during fasting.显示文摘 | Li X Lu F Wang JZ Gong CX | 2006 | 中国生物学文摘2006，20，9： | 12 |
| 4 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 5 | Activation of glycogen synthase kinase-3 induces Alzheimer-like tau hyperphosphorylation in rat hippocampus slices in culture.显示文摘 | Li X Lu F Tian Q Yang Y Wang Q Wang JZ | 2006 | 中国生物学文摘2006，20，9： | 9 |
| 6 | phase Ι clinical trail of Cefditoren pivoxil (M1207):pharmacokinetics in healthy volunteers显示文摘 | Li JT Hou F Lu H | 1997 | Drugs Exp Clin Res1997，23，56： | 4 |
| 7 | On-line measurement of the straightness of seamless steel pipe using machine vision technique显示文摘 | lU R S LI Y F YU Q | 2001 | Sensor and Actutors A-Physical2001，94，1： | 2 |
| 8 | Gene expression of manganese - contai- ning superoxidedismutase as a biomarker of manganese bioavailabihty for manganesesources in broilers显示文摘 | Luo X G Li S F Lu L | 2007 | Poult Sci2007，86，5： | 1 |
| 9 | Improvement of the survival of human autologous fat transplantation by using VEGF-transfected adipose- derived stem cells 显示文摘 | Lu F Li J Gao J | 2009 | Plast Reconstr Surg2009，124，5： | 1 |
| 10 | A Global Calibration Technique for High-accuracy 3D Measurement Systems显示文摘 | LU R S LI Y F | 2004 | Sensors and Actuators A: Physical2004，116，3： | 1 |
| 11 | Talanta显示文摘 | Li Y H Niu W F Lu J R | 2007 | 71:11242007，71，： | 1 |
| 12 | Can the soil seed bank contribute to revegetation of the drawdown zone in the Three Gorges Reservoir Region?显示文摘 | Lu Z J Li L F Jiang M X | | Plant Ecology0，209，1： | 1 |
| 13 | Morphology of polyvinylidene fluoride and its blend in thermally induced phase separation process 显示文摘 | LI X F LU X L | 2006 | J Appl Polym Sci2006，101，： | 1 |
| 14 | Synthesis and dye separation performance of ferromagnetic hierarchical porous carbon显示文摘 | Wang D W Li F Lu G Q | 2008 | Carbon2008，46，12： | 1 |
| 15 | Numerical study on the thermal performance of building wall and roof incorporating phase change material panel for passive cooling application显示文摘 | Kong X F Lu S L Li Y R | 2014 | Energy & Buildings2014，81，： | 1 |
| 16 | Corrosion behavior and porous structure formation of sputtered Cu-Zr nanostructured films 显示文摘 | Lu H B Li Y Wang F H | 2008 | Journal of Alloys and Compounds2008，465，12： | 1 |
| 17 | Electron transfer on an Electrode of Glucose Oxidase Immobilized in Polyaniline显示文摘 | Li C F Zheng D D | 1994 | J Electroanal Chem1994，364，12： | 1 |
| 18 | A study on the behavior of eccentrically compressed square concrete-filled steel tube columns 显示文摘 | Lu F W Li S P Sun Guojun | 2007 | Journal of Constructional Steel Research2007，63，7： | 1 |
| 19 | Functional properties of the Mail- lard reaction products of rice protein with sugar显示文摘 | Li Y Lu F Luo C | 2009 | Food Chemistry2009，117，1： | 1 |
| 20 | Bone morphogenetic protein induces efficienthematopoietic differentiation of rhesus monkey embryonic stem cells in vitro显示文摘 | Li F Lu S J Vida L | | 0，，02： | 1 |
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