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| 1 | In vitro derivation of functional insulin-producing cells from human embryonic stem cells显示文摘为人的胚胎的茎(ES ) 的自强和区别的能力细胞为对待类型 Idiabetes mellitus 为胰腺的贝它细胞的产生使他们成为潜在的来源。这里,我们报导一最新发展了并且有效方法,在aserum免费的系统执行了,区分进生产胰岛素的 cells.Activin A 的导致的人的 ES 房间它在起始的阶段被使用从人的 EScells 导致权威的内胚叶区别,是由权威的内胚叶标记 Sox17 和 Brachyury.Further 的表示检测了, all-trans retinoic 酸( RA )被用来支持胰腺的区别,由早胰腺的抄写因素 pdx1 和 hlxb9 的表示显示了。在成熟 inDMEM/F12 以后有 bFGF 和菸碱的没有浆液的媒介,区分的房间表示了小岛特定的标记象 C 肽,胰岛素,胰高血糖素和 glut2 那样。百分比 ofC-peptide-positive 房间超过了 15% 。由这些房间的胰岛素和 C 肽的分泌物在葡萄糖层次对应于变化。当移植了进肾的囊时, ofStreptozotocin (STZ ) 对待裸体老鼠,这些区分的人的 ES 房间熬过并且维持贝它房间标记基因的表示包括 C 肽, pdx1, glucokinase, nkx6.1, IAPP, pax6and Tcf1。百分之三十只移植裸体老鼠展出了 stableeuglycemia 的明显的恢复;并且改正的显型被支撑超过六个星期。我们的新方法为学习人的胰开发的机制提供一个有希望的试管内区别模特儿并且说明为类型 Idiabetes mellitus 的处理使用人的 ES 房间的潜力。 | Wei Jiang Yan Shi Dongxin Zhao Song Chen Jun Yong Jing Zhang Tingting Qing Xiaoning Sun Peng Zhang Mingxiao Ding Dongsheng Li Hongkui Deng | 2007 | Cell Research2007，17，4： | 38 |
| 2 | Relationship between the -455G/A and -148C/T polymorphisms in the beta-fibrinogen gene and cerebral infarction in the Xinjiang Uygur and Han Chinese populations显示文摘We sought to investigate the correlation between the -455G/A and -148C/T polymorphisms of the β-fibrinogen gene and plasma fibrinogen levels in patients with cerebral infarction and in healthy subjects among the Xinjiang Uygur and Han Chinese populations, by using polymerase chain reaction-restriction enzyme digestion analysis. Results showed that there were no statistically significant differences in the distributions of the -455G/A genotype and allele frequency between the Uygurs and the Han. Plasma fibrinogen levels in cerebral infarction patients among the Uygurs and the Han were higher than those among healthy subjects. In particular, the frequencies of the -455G/A AA and -148C/T TT genotypes were significantly higher than in healthy subjects. Individuals carrying the A or T allele had a higher incidence of cerebral infarction compared with those carrying the G or C allele. Our experimental findings indicate that the -148C/T and -455G/A polymorphisms are associated with cerebral infarction in Xinjiang Uygur and Han Chinese subjects. The susceptibility- conferring alleles are -148T and -455A, and the susceptibility-conferring genotype is -455G/A + AA. | Xiaoning Zhang Yanyun Li Xuebing Guo Lei Du Jianhua Ma | 2012 | Neural Regeneration Research2012，7，7： | 13 |
| 3 | Rock fragmentation mechanisms and an experimental study of drilling tools during high-frequency harmonic vibration显示文摘Resonance drilling is a new technology,still at the laboratory stage.It has great potential to improve rock fragmentation efficiency.We analyzed the amplitude-frequency characteristics of steady-state mechanical vibration excited by harmonic vibration in rocks and an apparatus was built to achieve high frequency vibration of rock.The influence of rock drillability,rotary speed,excitation frequency,and other parameters on the rate of penetration(ROP) in resonance drilling was analyzed.The results show that the rock drillability decreased with an increase in excitation frequency.When drilling with a large size drill bit,the ROP increased with excitation frequency.The ROP reached a maximum value at the resonant frequency of the rock.The ROP of the bit increased linearly with rotary speed when no vibration was applied on the rock and increased approximately exponentially when harmonic vibration was applied.In addition,the resonant frequency of the rock was changing during the process of rock fragmentation,so in order to achieve the desired resonance of the rock,it is necessary to determine an appropriate harmonic vibration excitation frequency. | Li Wei Yan Tie Li Siqi Zhang Xiaoning | 2013 | Petroleum Science2013，10，2： | 9 |
| 4 | A Novel Pneumatic Soft Gripper with a Jointed Endoskeleton Structure显示文摘In current research on soft grippers,pneumatically actuated soft grippers are generally fabricated using fully soft materials,which have the advantage of flexibility as well as the disadvantages of a small gripping force and slow response speed.To improve these characteristics,a novel pneumatic soft gripper with a jointed endoskeleton structure(E-Gripper)is developed,in which the muscle actuating function has been separated from the force bearing function.The soft action of an E-Gripper finger is performed by some air chambers surrounded by multilayer rubber embedded in the restraining fiber.The gripping force is borne and transferred by the rigid endoskeleton within the E-Gripper finger Thus,the gripping force and action response speed can be increased while the flexibility is maintained.Through experiments,the bending angle of each finger segment,response time,and gripping force of the E-Gripper have been measured,which provides a basis for designing and controlling the soft gripper The test results have shown that the maximum gripping force of the E-Gripper can be 35 N,which is three times greater than that of a fully soft gripper(FS-Gripper)of the same size.At the maximum charging pressure of 150 kPa,the response time is1.123 s faster than that of the FS-Gripper.The research results indicate that the flexibility of a pneumatic soft gripper is not only maintained in the case of the E-Gripper,but its gripping force is also obviously increased,and the response time is reduced.The E-Gripper thus shows great potential for future development and applications. | Zhaoping Wu Xiaoning Li Zhonghua Guo | 2019 | Chinese Journal of Mechanical Engineering2019，32，5： | 9 |
| 5 | Aurora A orchestrates entosis by regulating a dynamic MCAK-TIP150 interaction显示文摘 | PengXia JinhuaZhou Xiaoyu Song BingWu XingLiu Di Li Shuyuan Zhang Zhikai Wang H uijuan Yu Tarsha Ward Jiancun Zhang Yinmei Li Xiaoning Wang YongChen Zhen Guo Xuebiao Yao | 2014 | Journal of Molecular Cell Biology2014，8，3： | 9 |
| 6 | The synergistic effect of resveratrol in combination with cisplatin on apoptosis via modulating autophagy in A549 cells显示文摘几研究证明了有自然产品和化疗代理人的联合治疗能改进化疗代理人的 sensitivityand cytotoxicity。Resveratrol,一个自然产品,包括 antitumor andantiviral 活动,以及脉管的保护的效果有许多生物效果。这研究的目的是与 cisplatin 和涉及 A549 房间的潜在的 anticancer 机制在联合调查 resveratrol 的 synergistic anticancereffect。从数 Kit-8 和 isobolographic 分析的房间的 resultsobtained 表明了 resveratrol 和 cisplatin resultedin synergistic 的那联合在 A549 房间的细胞毒素的效果。从染色的 Hoechst 的结果,流动 cytometry 和西方的污点分析 suggestedthat resveratrol 提高了调停 cisplatin 的 apoptosis。同时, LC3-II 和 P62 层次和在有 cisplatin 的联合的 resveratrol 触发了的 autophagosomesuggested 的形成的变化 autophagy。更重要地,由 3-methyladeninemarkedly 禁止 autophagy 稀释了 resveratrol 和 cisplatin 的联合在 A549 房间引起的 apoptosis。一起拿,我们的 studyprovides resveratrol synergistically 与 cisplatin 相结合的第一条证据在 A549 房间经由 modulating autophagiccell 死亡导致 apoptosis。这些调查结果也帮助我们在肺癌症与 chemotherapyagents 在联合理解自然产品的角色。 | Song Hu Xiaolin Li Rongrong Xu Lingyun Ye Hui Kong Xiaoning Zeng Hong Wang Weiping Xie | 2016 | Acta Biochimica et Biophysica Sinica2016，48，6： | 9 |
| 7 | Refreezing of cast-in-place piles under various engineering conditions显示文摘In the construction of the Qinghai-Tibet Power Transmission Line(QTPTL), cast-in-place piles(CIPPs) are widely applied in areas with unfavorable geological conditions. The thermal regime around piles in permafrost regions greatly affects the stability of the towers as well as the operation of the QTPTL. The casting of piles will markedly affect the thermal regime of the surrounding permafrost because of the casting temperature and the hydration heat of cement. Based on the typical geological and engineering conditions along the QTPTL, thermal disturbance of a CIPP to surrounding permafrost under different casting seasons, pile depths, and casting temperatures were simulated. The results show that the casting season(summer versus winter) can influence the refreezing process of CIPPs, within the first 6 m of pile depth. Sixty days after being cast, CIPPs greater than 6 m in depth can be frozen regardless of which season they were cast, and the foundation could be refrozen after a cold season. Comparing the refreezing characteristics of CIPPs cast in different seasons also showed that, without considering the ground surface conditions, warm seasons are more suitable for casting piles. With the increase of pile depth, the thermal effect of a CIPP on the surrounding soil mainly expands vertically, while the lateral heat disturbance changes little. Deeper, longer CIPPs have better stability. The casting temperature clearly affects the thermal disturbance, and the radius of the melting circle increases with rising casting temperature. The optimal casting temperature is between 2 °C and 9 °C. | Lei Guo QiHao Yu XiaoNing Li XinBin Wang YongYu Yue | 2015 | Research in Cold and Arid Regions2015，7，4： | 8 |
| 8 | IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling显示文摘Interleukin-17 (IL-17 或 IL-17A ) 生产是 TH17 房间的一个特点,贡献多重自体免疫、煽动性的疾病的致病的 CD4+ T 淋巴细胞的一个新唯一的系。IL-17 受体(IL-17R 或 IL-17RA ) 为 IL-17 生物活动是必要的。新兴的数据建议 heteromeric 或 homomeric 受体建筑群的形成为 IL-17 发信号被要求。这里,我们证明孤儿受体 IL-17RD (Sef,类似的表示到 FGF 基因或 IL-17RLM ) 被联系并且有 IL-17R 的 colocalized。重要地, IL-17RD 调停 IL-17 发信号,用一个酶记者评估了由 24p3 的本国的倡导者开车, IL-17 目标基因。另外,主导否定地缺乏细胞内部的领域的 IL-17RD 异种压制 IL-17R-mediated IL-17 发信号。而且,象 IL-17R 一样的 IL-17RD 与 TRAF6 被联系,一个 IL-17R 下游的分子。这些结果显示 IL-17RD 是表明建筑群的 IL-17 受体的部分,因此为通过 heteromeric 或 homomeric 受体建筑群发信号的 IL-17 提供新奇证据。 | Zhili Rong Anan Wang Zhiyong Li Yongming Ren Long Cheng Yinghua Li Yinyin Wang Fangli Ren Xiaoning Zhang Jim Hu Zhijie Chang | 2009 | Cell Research2009，19，2： | 6 |
| 9 | EGb761抑制β-淀粉样蛋白_(25-35)片段诱导的神经元凋亡显示文摘以 β-淀粉样蛋白2 5 35片段 (β-AmyloidPeptide 2 5 35,β AP2 5 35)诱发原代培养小鼠皮层神经元出现凋亡作为阿尔采末症 (Alzheimer′sDisease ,AD)的离体模型 ,在此基础上观察了银杏叶提取物 (ExtractofleavesofGinkgobilobaL .,EGb76 1)及其主要活性成分内酯B(GinkgolideB ,GinB)对上述神经元损伤的对抗作用 .结果表明 ,EGb76 1能抑制 β AP2 5 35引起的神经元活性的下降和超微结构的改变 ,即抑制 β AP2 5 35诱导的神经元凋亡 ,从而为EGb76 1临床用于防治AD提供了实验依据 . | Gao Jing Zhu Li Zhao Xiaoning Zhang Zuxuan | 2001 | 南京大学学报(自然科学版)2001，37，2： | 6 |
| 10 | Internalization of NK cells into tumor cells requires ezrin and leads to programmed cell-in-cell death显示文摘细胞毒素的淋巴细胞是在有缺点的房间的有免疫力的反应和消除的组织的关键播放器。我们以前报导了生来的杀手(NK ) 房间进入目标肿瘤房间,导致在肿瘤房间以内的任何一个目标房间死亡或自我毁灭。然而,它留下了至于在成为主观以后的 NK 房间的命运逃犯并且 heterotypic cell-in-cell 过程最近是否与同型的 cell-in-cell 事件的不同,说出 entosis。这里,我们证明 NK 房间在肿瘤房间以内与 apoptosis 的最终的命运经历一个 cell-in-cell 过程并且表明成为主观过程要求肌动朊细胞骨架管理者, ezrin。设想 NK 房间怎么进入肿瘤房间,我们执行了 NK 房间成为主观的即时双颜色成像分析进肿瘤房间。令人惊讶地,大多数 NK 房间在他们的入口以后承诺规划房间死亡进肿瘤房间,它与在同型的 cell-in-cell 过程观察的 entosis 区别地不同。使内在化的 NK 房间的 apoptotic 房间死亡由 caspase 的激活是明显的 3 并且 DNA 破碎。而且,在成为主观以后的 NK 房间死亡被 caspase 禁止者稀释, Z-VAD-FMK,在肿瘤房间以内作为 NK 房间死亡的模式证实 apoptosis。决定为 NK 房间的入口必要的蛋白质因素进肿瘤房间,我们执行了基于 siRNA 的击倒的分析并且在 NK 房间成为主观发现了 ezrin 的一个关键角色。重要地, ezrin 的调停 PKA 的 phosphorylation 支持 NK 房间成为主观过程。我们的调查结果建议 ezrin 由管理 NK 房间成为主观进肿瘤房间的新奇规章的机制。 | Shan Wang Zhen Guo Peng Xia Tingting Liu Jufang Wang Shan Li Lihua Sun Jianxin Lu Qian Wen Mingqian Zhou Li Ma Xia Ding Xiaoning Wang Xuebiao Yao | 2009 | Cell Research2009，19，12： | 6 |
| 11 | Development of a Novel Parallel-spool Pilot Operated High-pressure Solenoid Valve with High Flow Rate and High Speed显示文摘High-pressure solenoid valve with high flow rate and high speed is a key component in an underwater driving system.However,traditional single spool pilot operated valve cannot meet the demands of both high flow rate and high speed simultaneously.A new structure for a high pressure solenoid valve is needed to meet the demand of the underwater driving system.A novel parallel-spool pilot operated high-pressure solenoid valve is proposed to overcome the drawback of the current single spool design.Mathematical models of the opening process and flow rate of the valve are established.Opening response time of the valve is subdivided into 4 parts to analyze the properties of the opening response.Corresponding formulas to solve 4 parts of the response time are derived.Key factors that influence the opening response time are analyzed.According to the mathematical model of the valve,a simulation of the opening process is carried out by MATLAB.Parameters are chosen based on theoretical analysis to design the test prototype of the new type of valve.Opening response time of the designed valve is tested by verifying response of the current in the coil and displacement of the main valve spool.The experimental results are in agreement with the simulated results,therefore the validity of the theoretical analysis is verified.Experimental opening response time of the valve is 48.3 ms at working pressure of 10 MPa.The flow capacity test shows that the largest effective area is 126 mm2 and the largest air flow rate is 2320 L/s.According to the result of the load driving test,the valve can meet the demands of the driving system.The proposed valve with parallel spools provides a new method for the design of a high-pressure valve with fast response and large flow rate. | DONG Dai LI Xiaoning | 2015 | Chinese Journal of Mechanical Engineering2015，28，2： | 6 |
| 12 | Theoretical and experimental study of the pulling force of jet bits in radial drilling technology显示文摘Radial drilling technology,of which the jet bit is the key device,is a research focus in the field of oil drilling and production.This paper establishes mechanical equations for jet bits and analyzes the hydroseal of backward jets in bottom holes.Meanwhile this paper establishes a mechanical equation for a high pressure hose and analyzes the axial force distribution.Laboratory experiments indicate that the flow rate,the angle between the backward nozzle axis and the jet bit axis,and the hole diameter are the major influencing factors;the generation of the pulling force is mainly due to the inlet pressure of the jet bit;the backward jets can significantly increase not only the pulling force but also the stability of jet bits.The pulling force would reach 8,376 N under experimental conditions,which can steadily pull the high-pressure hose forward. | Ruichang Guo Gensheng Li Zhongwei Huang Shouceng Tian Xiaoning Zhang Wei Wu | 2009 | Petroleum Science2009，6，4： | 5 |
| 13 | Solvent-free nanocasting toward universal synthesis of ordered mesoporous transition metal sulfide@N-doped carbon composites for electrochemical applications显示文摘Transition metal sulfides (TMSs) have a wide range of applications owing to their intriguing properties.Significant efforts have been devoted to nanostructuring TMSs to enhance their properties and performance,still there is a high need in general synthesis of TMS nanostructures.Herein,for the first time,a simple solvent free reactive nanocasting approach that integrates solid precursor loading,in-situ sulfuration and carbonization into a single heating step is developed for the universal synthesis of ordered mesoporous TMS@N-doped carbon composites (denoted as OM-TMS@NCs) with methionine (Met) and metal chlorides as the precursors and the mesoporous silica (SBA-15) as the hard template.A series of OM-TMS@NCs with a hexagonal mesostructure,ultra-high surface areas (430-754 m2·g-1),large pore volumes (0.85-1.32 cm3·g-1),and unique TMS stoichiometries,including MoS2,Fe7S8,Co9S8,NiS,Cu7S4 and ZnS,are obtained.Two distinct structure configurations,namely,highly dispersed ultrathin TMS nanosheets within NCs and TMS@NC co-nanowire arrays,can be obtained depending on different metals.The structure evolution of the OM-TMS@NCs over the solvent-free nanocasting process is studied in detail for a deep understanding of the synthesis.As demonstrations,these materials are promising for electrocatalytic hydrogen evolution reaction and lithium ion storage with high performances. | Jiahui Zhu Zhi Chen Lin Jia Yuqi Lu Xiangru Wei Xiaoning Wang Winston Duo Wu Na Han Yanguang Li Zhangxiong Wu | 2019 | Nano Research2019，12，9： | 5 |
| 14 | CMTM4 inhibits cell proliferation and migration via AKT, ERK1/2,and STAT3 pathway in colorectal cancer显示文摘CMTM4(CKLF-like MARVEL transmembrane domain containing 4),a potential tumor suppressor gene,is involved in several types of malignancies.It has been reported to be downregulated and exhibit anti-tumorigenic activities by regulating cell growth and cell cycle in clear cell renal cell carcinoma.It has also been identified as a tumor suppressor in hepatocellular carcinoma(HCC),and its negative expression is a risk factor for poor prognosis of HCC patients.In the present study,an integrated bioinformatics analysis based on The Cancer Genome Atlas(TCGA)database showed that CMTM4 was frequently reduced in colorectal cancer(CRC)and high expression of CMTM4 was associated with increased overall survival rates.Based on these findings,we adopted gain-of-function and lost-of-function strategies using SW480 and HT29 CRC cell lines which have relatively low and high endogenous CMTM4 levels,respectively.We observed impeded cell proliferation and migration upon overexpression of CMTM4 in SW480 cells,and the opposite effects were observed upon knockdown of CMTM4 in HT-29 cells.Cell signaling pathways essential for CRC progression were then examined,and the phosphorylation levels of AKT,ERK1/2,and STAT3 were found to be decreased by CMTM4 overexpression in SW480 cells and elevated by CMTM4 silencing in HT29 cells.Their inhibitors were used to validate that the three signaling pathways contributed to the inhibitory effects of CMTM4 on CRC cells.Taken together,our results suggest that CMTM4 plays a tumor suppressive role in CRC. | Hui Xue Ting Li Pingzhang Wang Xiaoning Mo Hejun Zhang Shigang Ding Dalong Ma Wenping Lv Jing Zhang Wenling Han | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 5 |
| 15 | Anisotropic corrosion behavior of sintered(Ce_(0.15)Nd_(0.85))_(30)Fe_(bal)B permanent magnets显示文摘In this work, the corrosion behavior of the surface parallel to the oriented c-axis(c‖) and perpendicular to c-axis(c⊥)in the(Ce_(0.15)Nd_(0.85))_(30)Fe_(bal)B dual main phase magnet was studied. With the addition of Ce,the volume fraction of RE-rich phase shown in the backscattered electron(BSE) images is basically approximate on the two surfaces. The free corrosion potential(E_(corr)) of the c‖ surface is more negative,which shows the worse corrosion resistance from the perspective of thermodynamics. While the reaction kinetics parameters with the smaller free corrosion current(i_(corr)) and the larger transfer resistance(R_(ct)) react the opposite conclusion in 3.5 wt% NaCl solution. Moreover, the c‖ surface performs smaller ions concentration of corrosion product and less damaged corrosion morphology compared to c⊥ surface after the free corrosion. The inconformity is not affected by the RE-rich phase, but by the anisotropy of the grains that the c‖ surface has larger density of the atoms and the lower ratio of(Nd, Ce)/Fe. | Xiaoning Shi Minggang Zhu Dong Zhou Zheng Jing Meiling Zhang Wei Li | 2019 | Journal of Rare Earths2019，37，3： | 5 |
| 16 | Global crustal movement and tectonic plate boundary deformation constrained by the ITRF2008显示文摘On the basis of the newly released International Terrestrial Reference Frame(ITRF2008) by the International Earth Rotation Service(IERS),a new global plate model ITRF2008 plate for the major plates is established.This ITRF2008-derived model is analyzed in comparison with NNR-NUVEL1A model,which is mainly based on geological and geophysical data.The Eurasia and Pacific plates display obvious differences in terms of the velocity fields derived from the two plate motion models.Plate acceleration is also introduced to characterize the differences of the two velocity fields which obtained from ITRF2008-plate and NNR-NUVEL1A models for major individual plates.The results show that the Africa,South America and Eurasia plates are undergoing acceleration,while the North America and Australia plates are in the state of deceleration motion. | Zhu Ze Meng Guojie Su Xiaoning Wu Jicang Li Jean Xiaojing | 2012 | Geodesy and Geodynamics2012，3，3： | 4 |
| 17 | Impact of National Centralized Drug Procurement Policy on Antiviral Utilization and Expenditure for Hepatitis B in China显示文摘Background and Aims:The National Centralized Drug Procurement(NCDP)policy was launched in China's Mainland in April 2019,with entecavir(ETV)and tenofovir disoproxil fumarate(TDF)being included in the procurement list.We conducted the current study to investigate the impact of the NCDP policy on the utilization and expenditures of antiviral therapy for chronic hepatitis B(CHB)in China.Methods:Procurement records,including monthly purchase volume,expenditure,and price of nucleos(t)ide analogs(NAs),were derived from the National Healthcare Security Administration from April 2018 to March 2021.The changes in volumes and expenditures of the first-line NAs and bid-winning products were calculated.The effects of price,volume,and structure related to drug expenditure were calculated by the Addis and Magrini(AM)Index System Analysis.Results:The purchase volume of NAs significantly increased from 134.3 to 318.3 million DDDs,whereas the expenditure sharply decreased from 1,623.41 to 490.43 million renminbi(RMB)or 241.94 to 73.09 million US dollars(USD).The proportions of firstline NAs rose from 72.51%(ETV:69.00%,TDF:3.51%)to 94.97%(ETV:77.42%,TDF:17.55%).AM analysis showed that the NCDP policy decreased the expenditure of all NAs(S=0.91)but increased that of the first-line NAs in the bidwinning list(S=1.13).Assuming the population size of CHB patients remains stable and a compliance rate of≥75%,the proportion of CHB patients receiving first-line antiviral therapy would increase from 6.36–8.48%to 11.56–15.41%.Conclusions:The implementation of the NCDP policy significantly increased the utilization of first-line NAs for CHB patients at a lower expenditure.The findings provided evidence for optimizing antiviral therapy strategy and allocating medical resources in China. | Xinyu Zhao Min Li Hao Wang Xiaoqian Xu Xiaoning Wu Yameng Sun Canjian Ning Bingqiong Wang Shuyan Chen Hong You Jidong Jia Yuanyuan Kong | 2022 | Journal of Clinical and Translational Hepatology2022，10，3： | 4 |
| 18 | Ionomycin ameliorates hypophosphatasia via rescuing alkaline phosphatase deficiency-mediated L-type Ca^(2+) channel internalization in mesenchymal stem cells显示文摘The loss-of-function mutations in the ALPL result in hypophosphatasia(HPP), an inborn metabolic disorder that causes skeletal mineralization defects. In adults, the main clinical features are early loss of primary or secondary teeth, osteoporosis, bone pain,chondrocalcinosis, and fractures. However, guidelines for the treatment of adults with HPP are not available. Here, we show that ALPL deficiency caused a reduction in intracellular Ca2+ influx, resulting in an osteoporotic phenotype due to downregulated osteogenic differentiation and upregulated adipogenic differentiation in both human and mouse bone marrow mesenchymal stem cells(BMSCs). Increasing the intracellular level of calcium in BMSCs by ionomycin treatment rescued the osteoporotic phenotype in alpl+/- mice and BMSC-specific(Prrx1-alpl-/-) conditional alpl knockout mice. Mechanistically, ALPL was found to be required for the maintenance of intracellular Ca2+ influx, which it achieves by regulating L-type Ca2+ channel trafficking via binding to the α2δsubunits to regulate the internalization of the L-type Ca2+ channel. Decreased Ca2+ flux inactivates the Akt/GSK3β/β-catenin signaling pathway, which regulates lineage differentiation of BMSCs. This study identifies a previously unknown role of the ectoenzyme ALPL in the maintenance of calcium channel trafficking to regulate stem cell lineage differentiation and bone homeostasis. Accelerating Ca2+ flux through L-type Ca2+ channels by ionomycin treatment may be a promising therapeutic approach for adult patients with HPP. | Bei Li Xiaoning He Zhiwei Dong Kun Xuan Wei Sun Li Gao Shiyu Liu Wenjia Liu Chenghu Hu Yimin Zhao Songtao Shi Yan Jin | 2020 | Bone Research2020，8，2： | 4 |
| 19 | Experimental Research of Force Feedback Dataglove Based on Pneumatic Artificial Muscle显示文摘Force feedback dataglove is an important interface of human-machine interaction between manipulator and virtual assembly system,which is in charge of the bidirectional transmission of movement and force information between computer and operator. The exoskeleton force feedback dataglove is designed taking the pneumatic artificial muscle as actuator,meanwhile,its structure and work principle are introduced,and the force control problem is analyzed and researched by experiment. The mathematic model of grasping rigid object for finger is established. Considering the friction of tendon-sheath system and finger deformation,the closed-loop force control for a single joint,a single finger and multi-fingers are studied respectively by the feedforward proportional-integral(PI) control method with variable arguments. On the premise of the force smoothness,the control error of the force exerted on the finger joint is in the range of ±0.25 N,which meets the requirement of force feedback. By experimental analysis,the reason of force fluctuation is that the finger joint has a small amplitude quiver,and the consistent change tendency of the force between proximal interphalangeal(PIP) joint and distal interphalangeal(DIP) joint results from their angle coupling relationship. | SUN Zhongsheng BAO Gang LI Xiaoning | 2009 | Chinese Journal of Mechanical Engineering2009，22，4： | 4 |
| 20 | Carbon isotope composition and comparison of Lower Triassic marine carbonate rocks from Southern Longmenxia section in Guang'an, Sichuan Basin显示文摘The Early Triassic was a period of ecological restoration for the earth's system after the end-Permian mass extinction.Geochemical records linked to the variations in marine ecosystems during this period have attracted the interest of geologists for many years.Based on petrographic analysis of samples and evaluations of their reliability as proxies for original seawater,this study investigated the carbon and oxygen isotopic compositions of 350 carbonate rock samples from the Lower Triassic(and adjacent strata)in the southern Longmenxia section of Guang'an,eastern Sichuan Basin.The results indicate that the Triassic carbonate rocks from the southern Longmenxia section favorably preserved the original seawater'sδ^(13)C signal.Furthermore,carbon and oxygen isotopic compositions are found to be poorly correlated,with a determination coefficient as low as 0.0205 and only 44 rock samples show results of Mn/Sr>2 and/orδ^(18)0<-6.5‰.The complete carbon isotopic curve for the Lower Triassic is established using the data from the remaining 306 samples with Mn/Sr<2 and/orδ^(18)O>-6.5‰.This curve presents favorable comparability on a global scale,specifically in theδ^(13)C minima near the Permian-Triassic boundary,at the top of the Jia1 and within the the Jia3,as well as in theδ^(13)C maxima at the tops of the Ye1 and Ye4,at the base of the Jia2 and at the top of the Ye3.The peaks and troughs corresponding to these maxima and minima all reflect global signals.By comparing these results to previous research on coeval carbon isotopic curves established within the chronostratigraphic framework,the ages of these critical evolution points are determined.The results show that the Ye1 roughly corresponds to the Griesbachian substage;the Ye2 through Ye4 correspond to the Dienerian substage;Jial corresponds to the Smithian substage;from the Jia2 to the lower part of the Jia4 correspond to the Spathian substage;and the middle and upper parts of the Jia4 belongs to the Aegean Substage of the Middle Triassic.Around the boundary between the Jial and Jia2(which represents the Smithian-Spathian boundary(SSB)),the value ofδ^(13)C increases rapidly from-0.911‰to 3.679‰.The span during which the seawater's carbon isotope experiences this drastic change may be less than36 kyr.The oxygen isotope,which is more sensitive to sedimentary environmental changes,exhibits changes prior to the carbon isotope near the SSB,indicating a significant increase in the salinity of the seawater before a sharp rise in the carbon isotope;this event leads to the formation of evaporites and dolomites. | HUANG SiJing HUANG KeKe ZHONG YiJiang LI XiaoNing MAO XiaoDong HU ZuoWei LIU SiBing ZHANG Meng WU WenHui | 2017 | Science China Earth Sciences2017，60，1： | 4 |
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