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| 1 | Biochemical properties of norepinephrine as a kind of neurotransmitter secreted by bone marrow-derived neural stem cells induced and differentiated in vitro显示文摘BACKGROUND: It has been proved by many experimental studies from the aspects of morphology and immunocytochemistry in recent years that bone marrow stromal cells (BMSCs) can in vitro induce and differentiate into the cells possessing the properties of nerve cells. But the functions of BMSCs-derived neural stem cells(NSCs) and the differentiated neuron-like cells are still unclear. OBJECTIVE: To observe whether bone marrow-derived NSCs can secrete norepinephrine (NE) under the condition of in vitro culture, induce and differentiation, and analyze the biochemical properties of BMSCs-derived NSCs. DESIGN: A non-randomized and controlled experimental observation. SETTING: Institute of Neuromedicine of Chinese PLA, Zhujiang Hospital, Southern Medical University. MATERIALS: This experiment was carried out in the Institute of Neuromedicine of Chinese PLA, Zhujiang Hospital, Southern Medical University. The bone marrow used in the experiment was collected from 1.5- month-old healthy New Zealand white rabbits. METHODS: This experiment was carried out in the Institute of Neuromedicine of Chinese PLA, Zhujiang Hospital, Southern Medical University. The bone marrow used in the experiment was collected from 1.5 month-old healthy New Zealand white rabbits. BMSCs of rabbits were isolated and performed in vitro culture, induce and differentiation with culture medium of NSCs and differentiation-inducing factor, then identified with immunocytochemical method. Experimental grouping: ①Negative control group: L-02 hepatic cell and RPMI1640 culture medium were used. ② Background culture group: Only culture medium of NSCs as culture solution was added into BMSCs to perform culture, and 0.1 volume fraction of imported fetal bovine serum was supplemented 72 hours later. ③Differentiation inducing factor group: After culture for 72 hours, retinoic acid and glial cell line-derived neurotrophic factors were added in the culture medium of BMSCs and NSCs as corresponding inducing factors. The level of NE in each group was detected on the day of culture and 5, 7, 14 and 20 days after culture with high performance liquid chromatography(HPLC). The procedure was conducted 3 times in each group.Standard working curve was made according to the corresponding relationship of NE concentration and peak area. The concentration of NE every 1×107 cells was calculated according to standard curve and cell counting. MAIN OUTCOME MEASURES: The level of NE of cultured cells was detected with HPLC; immunocytochemistrical identification of Nestin and neuron specific nuclear protein was performed. RESULTS: ① On the 14th day after cell culture, BMSCs turned into magnus and round cells which presented Nestin-positive antigen, then changed into neuron-like cells with long processus and presented neuron specific nuclear protein -positive antigen at the 20th day following culture. ② The ratio of NE concentration and peak area has good linear relationship, and regression equation was Y=1.168 36+0.000 272 8X,r=0.998 4.Coefficient variation (CV) was < 5% and the recovery rate was 92.39%(Y referred to concentration and X was peak area).③NE was well detached within 10 minutes under the condition of this experiment. ④ NE was detected in NSCs and their culture mediums, which were cultured for 7, 14 and 20 days respectively, but no NE in BMSCs, NSCs-free culture medium and L-02 hepatic cell which were as negative control under the HPLC examination. Analysis of variance showed that the level of NE gradually increased following the elongation of culture time (P < 0.01). No significant difference in the level of NE existed at the same time between differentiation inducing factor group and basic culture group(P > 0.05). CONCLUSION: BMSCs of rabbits can proliferate in vitro and express Nestin antigen; They can differentiate into neuron-like cells, express specific neucleoprotein of mature neurons, synthesize and secrete NE as a kind of neurotransmitter. | Jianrong Chen Xiaodan Jiang Ruxiang Xu Peng Jin Yuxi Zou Lianshu Ding | 2006 | Neural Regeneration Research2006，1，2： | 3 |
| 2 | High-Resolution Mesoscale Analysis Data from the South China Heavy Rainfall Experiment (SCHeREX): Data Generation and Quality Evaluation显示文摘In this study, the observational data acquired in the South China Heavy Rainfall Experiment (SCHeREX) from May to July 2008 and 2009 were integrated and assimilated with the US National Oceanic and Atmospheric Administration's (NOAA) Local Analysis and Prediction System (LAPS; information available online at http://gffzzc2aa3fc9434744efhkxvwxnwfpp5x6qqx.ffgz.tsg.suse.edu.cn). A high-resolution mesoscale analysis dataset was then generated at a spatial resolution of 5 km and a temporal resolution of 3 h in four observational areas: South China, Central China, Jianghuai area, and Yangtze River Delta area. The quality of this dataset was evaluated as follows. First, the dataset was qualitatively compared with radar reflectivity and TBB image for specific heavy rainfall events so as to examine its capability in reproduction of mesoscale systems. The results show that the SCHeREX analysis dataset has a strong capability in capturing severe mesoscale convective systems. Second, the mean deviation and root mean square error of the SCHeREX mesoscale analysis fields were analyzed and compared with radiosonde data. The results reveal that the errors of geopotential height, temperature, relative humidity, and wind of the SCHeREX analysis were within the acceptable range of observation errors. In particular, the average error was 45 m for geopotential height between 700 and 925 hPa, 1.0-1.1°C for temperature, less than 20% for relative humidity, 1.5-2.0 m s-1 for wind speed, and 20-25° for wind direction. The above results clearly indicate that the SCHeREX mesoscale analysis dataset is of high quality and sufficient reliability, and it is applicable to refined mesoscale weather studies. | NI Yunqi CUI Chunguang LI Hongli PENG Juxiang QIU Xuexing ZHANG Yanxia XU Xiaolin GAO Mei JIE Lianshu ZHANG Wenhua | 2011 | Acta meteorologica Sinica2011，25，4： | 2 |
| 3 | A study on the vegetation in the east side of Helan Mountain显示文摘 | Jiang Yuan Kang Muyi Liu Shuo Tian Lianshu Lei Mingde | 2000 | Plant Ecology2000，，2： | 1 |
| 4 | Conscientiously Sum Up Reform & Development Experience to Create More Refulgence of Accounting Theories显示文摘 | Jin Lianshu | | 0，，： | 1 |
| 5 | Jumping problem in a nonlinear beam equation 显示文摘 | Yin Lun Pei Lianshu and Jin Zhengguo | 2001 | Journal of Yanbian University2001，27，1： | 1 |
| 6 | Uniqueness theorems in the ambrosetti type semilinear wave equation 显示文摘 | Jin Zhengguo Pei Lianshu and Zhao Meihua | 2000 | Journal of Yanbian University2000，26，1： | 1 |
| 7 | Analysis of gene mutations in Chinese patients with maple syrup urine disease显示文摘 | Nan Yang Lianshu Han Xuefan Gu Jun Ye Wenjuan Qiu Huiwen Zhang Zhuwen Gong Yafen Zhang | 2012 | Molecular Genetics and Metabolism2012，，4： | 1 |
| 8 | The implementation of DC in a loosely-coupled distributed environment 显示文摘 | TIAN Laisheng HUANG Lianshu TAO Jie | 1995 | Chinese J Advanced Software Research1995，2，3： | 1 |
| 9 | Newborn screening for genetic disorders:Current status and prospects for the future显示文摘Newborn screening(NBS)is a public health service aimed at identifying infants with severe genetic disorders,thus providing effective treatment early enough to prevent or ameliorate the onset of symptoms.Current NBS uses biochemical analysis of dried blood spots,predominately with timeresolved fluorescence immunoassay and tandem mass spectrometry,which produces some false positives and false negatives.The application of enzymatic activity-based testing technology provides a reliable screening method for some disorders.Genetic testing is now commonly used for secondary or confirmatory testing after a positive result in some NBS programs.Recently,next-generation sequencing(NGS)has emerged as a robust tool that enables large panels of genes to be scanned together rapidly.Rapid advances in NGS emphasize the potential for genomic sequencing to improve NBS programs.However,some challenges still remain and require solution before this is applied for population screening. | Si Ding Lianshu Han | 2022 | Pediatric Investigation2022，6，4： | 0 |
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