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| 1 | Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants显示文摘 | Yubing He Min Zhu Lihao Wang Junhua Wu Qiaoyan Wang Rongchen Wang Yunde Zhao | 2018 | Molecular Plant2018，11，9： | 18 |
| 2 | Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing显示文摘Development of tools for targeted modifications of specific DNA sequences in plants is of great importance to basic plant biology research as well as crop improvement.The ability to cut DNA at specific locations in the genome to generate doublestrand breaks(DSBs)in vivo is a prerequisite for any genome editing efforts.Several engineered nucleases including zinc finger nucleases(ZFNs)and transcriptional activator-like nucleases(TALENs)have been successfully used to edit genomes.However, | Yubing He Tao Zhang Ning Yang Meilian Xu Lang Yan Lihao Wang Rongchen Wang Yunde Zhao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 3 | An Organic Solvent-Assisted Intercalation and Collection (OAIC) for Ti_(3)C_(2)T_(x) MXene with Controllable Sizes and Improved Yield显示文摘A good method of synthesizing Ti_(3)C_(2)T_(x)(MXene)is critical for ensuring its success in practical applications,e.g.,electromagnetic interference shielding,electrochemical energy storage,catalysis,sensors,and biomedicine.The main concerns focus on the moderation of the approach,yield,and product quality.Herein,a modified approach,organic solvent-assisted intercalation and collection,was developed to prepare Ti_(3)C_(2)T_(x) flakes.The new approach simultaneously solves all the concerns,featuring a low requirement for facility(centrifugation speed<4000 rpm in whole process),gram-level preparation with remarkable yield(46.3%),a good electrical conductivity(8672 S cm^(−1)),an outstanding capacitive performance(352 F g^(−1)),and easy control over the dimension of Ti_(3)C_(2)T_(x) flakes(0.47–4.60μm^(2)).This approach not only gives a superb example for the synthesis of other MXene materials in laboratory,but sheds new light for the future mass production of Ti_(3)C_(2)T_(x) MXene. | Danyao Qu Yingying Jian Lihao Guo Chen Su Ning Tang Xingmao Zhang Wenwen Hu Zheng Wang Zhenhuan Zhao Peng Zhong Peipei Li Tao Du Hossam Haick Weiwei Wu | 2021 | Nano-Micro Letters2021，13，11： | 2 |
| 4 | Identification of two novel mutations in the GALNT3 gene in a Chinese family with hyperphosphatemic familial tumoral calcinosis显示文摘Hyperphosphatemic familial tumoral calcinosis(HFTC) is a rare, autosomal recessive genetic disease.This disease is characterized by the progressive calcification of soft tissues leading to symptoms of pressure and hyperphosphatemia but normal concentrations of serum calcium with or without an elevation of1,25-dihydroxyvitamin D3 levels.HFTC is caused by loss-of-function mutations in the GALNT3, FGF23 or KL genes. Here, we identified two novel mutations in the GALNT3 gene in a Chinese family with HFTC.Identification of a novel genotype in HFTC provides clues for understanding the phenotype–genotype relationships in HFTC and may assist not only in the clinical diagnosis of HFTC but also in the interpretation of the genetic information used for prenatal diagnosis and genetic counseling. | Lihao Sun Lin Zhao Lianiun Du Peipei Zhang Minjia Zhang Min Li Tingting Liu Lei Ye Bei Tao Hongyan Zhao Jianmin Liu Xiaoyi Ding | 2016 | Bone Research2016，4，4： | 2 |
| 5 | Preferential orientation of tracer spheroids in turbulent channel flow显示文摘Axis-symmetric spheroids, such as rod-like and disk-like particles, have been found to orient preferentially in near-wall turbulence by both experiment and numerical simulation. In current work we examined the orientation of inertialess spheroids in a turbulent channel flow at medium friction Reynolds number Reτ=100 given based on the half of channel height. Both elongated prolate spheroid and flat oblate spheroid are considered and further compared with the reference case of spherical particle. The statistical results show that in near wall region the prolate spheroids tend to align in the streamwise direction while the oblate spheroids prefer to orient in the wallnormal direction, which are consistent with earlier observation in low Reynolds number (Reτ=180)wall turbulence. Around the channel center we found that the orientation of spheroids is not fully isotropic, even though the fluid vorticity are almost isotropic. The mechanism that gives rise to such particle orientations in wall-turbulence has been found to be related to fluid Lagrangian stretching and compression (Zhao and Andersson 2016). Therefore, we computed the left Cauchy-Green strain tensor along Lagrangian trajectories of tracer spheroids in current flow field and analyzed the fluid Lagrangian stretching and compression. The results indicated that, similar to the earlier observations, the directions of the Lagrangian stretching and compression in near-wall region are in the streamwise and wall-normal directions, respectively. Furthermore, cross over the channel the prolate spheroids aligned with the direction of Lagrangian stretching but oblate spheroids oriented with the direction of Lagrangian compression. The weak anisotropy of orientations of fluid Lagrangian stretching and compression observed at the channel center could be the reason for the aforementioned modest anisotropic orientation of spheroids in channel central region. | Yucheng Jie Lihao Zhao Chunxiao Xu Helge I. Andersson | 2019 | Theoretical & Applied Mechanics Letters2019，9，3： | 1 |
| 6 | Fabrication of a bio-instructive scaffold conferred with a favorable microenvironment allowing for superior implant osseointegration and accelerated in situ vascularized bone regeneration via type H vessel formation显示文摘The potential translation of bio-inert polymer scaffolds as bone substitutes is limited by the lack of neovascularization upon implantation and subsequently diminished ingrowth of host bone,most likely resulted from the inability to replicate appropriate endogenous crosstalk between cells.Human umbilical vein endothelial cell-derived decellularized extracellular matrix(HdECM),which contains a collection of angiocrine biomolecules,has recently been demonstrated to mediate endothelial cells(ECs)-osteoprogenitors(OPs)crosstalk.We employed the HdECM to create a PCL(polycaprolactone)/fibrin/HdECM(PFE)hybrid scaffold.We hypothesized PFE scaffold could reconstitute a bio-instructive microenvironment that reintroduces the crosstalk,resulting in vascularized bone regeneration.Following implantation in a rat femoral bone defect,the PFE scaffold demonstrated early vascular infiltration and enhanced bone regeneration by microangiography(μ-AG)and micro-computational tomography(μ-CT).Based on the immunofluorescence studies,PFE mediated the endogenous angiogenesis and osteogenesis with a substantial number of type H vessels and osteoprogenitors.In addition,superior osseointegration was observed by a direct host bone-PCL interface,which was likely attributed to the formation of type H vessels.The bio-instructive microenvironment created by our innovative PFE scaffold made possible superior osseointegration and type H vessel-related bone regeneration.It could become an alternative solution of improving the osseointegration of bone substitutes with the help of induced type H vessels,which could compensate for the inherent biological inertness of synthetic polymers. | Yijun He Wenhao Wang Shaozhang Lin Yixi Yang Lizhi Song Yihan Jing Lihao Chen Zaopeng He Wei Li Ao Xiong Kelvin W.K.Yeung Qi Zhao Yuan Jiang Zijie Li Guoxian Pei Zhi-Yong Zhang | 2022 | Bioactive Materials2022，7，3： | 1 |
| 7 | Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants显示文摘Elimination of the CRISPR/Cas9 constructs in edited plants is a prerequisite for assessing genetic stability, conducting phenotypic characterization, and applying for commercialization of the plants. However, removal of the CRISPR/Cas9 transgenes by genetic segregation and by backcross is laborious and time consuming. We previously reported the development of the transgene killer CRISPR(TKC) technology that uses a pair of suicide genes to trigger self-elimination of the transgenes without compromising gene editing efficiency. The TKC technology enables isolation of transgene-free CRISPR-edited plants within a single generation, greatly accelerating crop improvements. Here, we presented two new TKC vectors that show great efficiency in both editing the target gene and in undergoing self-elimination of the transgenes. The new vectors replaced the CaMV35 S promoter used in our previous TKC vector with two rice promoters to drive one of the suicide genes, providing advantages over our previous TKC vector under certain conditions. The vectors reported here offered more options and flexibility to conduct gene editing experiments in rice. | HE Yubing ZHU Min WANG Lihao WU Junhua WANG Qiaoyan WANG Rongchen ZHAO Yunde | 2019 | Rice science2019，26，2： | 1 |
| 8 | Fertilization and clear-cutting effects on greenhouse gas emissions of pinewood nematode damaged Masson pine plantation显示文摘Introduction:Management practices are essential for maintaining forest ecological functions under increasing diseases and pest disasters.The effects of nitrogen fertilization(NF)and clear-cutting(CC)on the soil microbial community structure and greenhouse gases emission were investigated of pinewood nematodes(Bursaphelenchus xylophilus)-infected Masson pine(Pinus massoniana)plantations.Outcomes:CC increased the soil microbial biomass carbon(SMBC)and soil microbial biomass nitrogen(SMBN)contents relative to the control(CK).NF increased the SMBN but had no significant effect on the SMBC content.The total fungal and bacterial abundances increased in the CC treatment compared with the CK,but there was no significant difference between the NF and CK.The cumulative soil CO_(2)emission(-2.35 t C·hm^(-2))was higher than that of CK(1.65 t C·hm^(-2))in summer,and the cumulative annual N_(2)O emission(16.90 kg N·hm^(-2)yr^(-1))of NF was approximately 47 times of CK(0.36 t N·hm^(-2)·yr^(-1)).CC increased the CO_(2)flux(-2.21 t C·hm^(-2))in summer but have no significantly effects on N_(2)O emission.Conclusion:These results indicated that NF and CC practices changed forest soil microbial community structure and affected soil greenhouse gas emissions in pinewood nematodes-infected Masson pine plantations.The CO_(2)emission rates increased in the NF and CC treatments,which reduced the carbon sequestration function of forests and had a negative impact on climate change. | Xue Zhang Zeyao Zhao Tong Chen Tianyu Zhao Lihao Song Li Mei | 2021 | Ecosystem Health and Sustainability2021，7，1： | 1 |
| 9 | Lighted up by hydrogen-bonding: luminescence behavior and applications of AIEgen-doped interpenetrating network hydrogel显示文摘The interaction between the aggregation-induced emissive(AIE) luminogens(AIEgen) and the polymer is the key scientific question in the design of functional AIE hydrogels. In this study, we report the AIE behavior of a poly(acrylic acid)(PAAc) and poly(acrylamide)(PAAm) interpenetrating network(IPN) hydrogel doped with tetra-cationic tetraphenylethene(TCTPE). The cationic AIEgen can bind with PAAc through ionic interaction, while PAAc and PAAm chains can associate with each other through hydrogen-bonds(H-bonds). These two interactions can restrict the intramolecular rotation and thus activate the luminescence of the AIEgen. The PAAc-PAAm H-bonds can be broken by increasing temperature or p H, restoring the intramolecular rotation of the AIEgen and quenching the fluorescence of the hydrogel. Therefore, the TCTPE-doped IPN hydrogel is designed as temperature-and p H-sensitive displayers which can record information imprinted by photo-printing or iono-printing with good switchability and reversibility. Another application of this TCTPE-doped hydrogel is demonstrated as a luminescent soft actuator, which has fast shape deformation and editable fluorescence pattern. The above results reveal a pathway to tune the emission behavior through tuning polymer-polymer and polymer-AIEgen interactions, which may inspire new design strategies of aggregation-induced emissive polymers and broaden their applications. | Mengdi Xu Luqin Hua Lihao Gong Jianlei Lu Jinhui Wang Chuanzhuang Zhao | 2021 | Science China Chemistry2021，64，10： | 1 |
| 10 | Antioxidant-enriched autologous biogel promoted diabetic wound healing by remodeling inherent posttraumatic inflammatory patterning and restoring compromised microenvironment homeostasis显示文摘Successful wound healing depends on the reconstruction of proper tissue homeostasis,particularly in the posttraumatic inflammatory tissue microenvironment.Diabetes jeopardizes tissues’immune homeostasis in cutaneous wounds,causing persistent chronic inflammation and cytokine dysfunction.Previously,we developed an autologous regeneration factor(ARF)technology to extract the cytokine composite from autologous tissue to restore immune homeostasis and promote wound healing.However,treatment efficacy was significantly compromised in diabetic conditions.Therefore,we proposed that a combination of melatonin and ARF,which is beneficial for proper immune homeostasis reconstruction,could be an effective treatment for diabetic wounds.Our research showed that the utilization of melatonin-mediated ARF biogel(AM gel)promoted diabetic wound regeneration at a more rapid healing rate.RNA-Seq analysis showed that AM gel treatment could restore more favorable immune tissue homeostasis with unique inflammatory patterning as a result of the diminished intensity of acute and chronic inflammation.Currently,AM gel could be a novel and promising therapeutic strategy for diabetic wounds in clinical practice through favorable immune homeostatic reconstructions in the tissue microenvironment and proper posttraumatic inflammation patterning. | Yixi Yang Le Wang Yonglin Zhou Yijun He Shaozhang Lin Yuwei Zeng Yunhe Zhou Wei Li Zaopeng He Qi Zhao Lihao Chen Zijie Li Wenhao Wang Zhi-Yong Zhang | 2022 | Regenerative Biomaterials2022，9，1： | 0 |
| 11 | Cadmium removal mechanistic comparison of three Fe-based nanomaterials: Water-chemistry and roles of Fe dissolution显示文摘Cadmium(Cd)is a common toxic heavy metal in the environment.Taking Cd(II)as a target contaminant,we systematically compared the performances of three Fe-based nanomaterials(nano zero valent iron,nZVI;sulfidated nZVI,S-nZVI;and nano FeS,nFeS)for Cd immobilization under anaerobic conditions.Effects of nanomaterials doses,initial pH,co-existing ions,and humic acid(HA)were examined.Under identical conditions,at varied doses or initial pH,Cd(II)removal by three materials followed the order of S-nZVI>nFeS>nZVI.At pH 6,the Cd(II)removal within 24 hours for S-nZVI,nFeS,and nZVI(dose of 20 mg/L)were 93.50%,89.12%and 4.10%,respectively.The fast initial reaction rate of nZVI did not lead to a high removal capacity.The Cd removal was slightly impacted or even improved with co-existing ions(at 50 mg/L or 200 mg/L)or HA(at 2 mg/L or 20 mg/L).Characterization results revealed that nZVI immobilized Cd through coprecipitation,surface complexation,and reduction,whereas the mechanisms for sulfidated materials involved replacement,coprecipitation,and surface complexation,with replacement as the predominant reaction.A strong linear correlation between Cd(II)removal and Fe(II)dissolution was observed,and we proposed a novel pseudo-second-order kinetic model to simulate Fe(II)dissolution. | Xiaoge Huang Lihao Chen Ziqi Ma Kenneth C.Carroll Xiao Zhao Zailin Huo | 2022 | Frontiers of Environmental Science & Engineering2022，16，12： | 0 |
| 12 | Pepper variome reveals the history and key loci associated with fruit domestication and diversification显示文摘Pepper(Capsicum spp.)is an important vegetable crop that provides a unique pungent sensation when eaten.Through construction of a pepper variome map,we examined the main groups that emerged during domestication and breeding of C.annuum,their relationships and temporal succession,and the molecular events underlying the main transitions.The results showed that the initial differentiation in fruit shape and pungency,increase in fruit weight,and transition from erect to pendent fruits,as well as the recent appearance of large,blocky,sweet fruits(bell peppers),were accompanied by strong selection/fixation of key alleles and introgressions in two large genomic regions.Furthermore,we identified Up,which encodes a BIG GRAIN protein involved in auxin transport,as a key domestication gene that controls erect vs pendent fruit orientation.The up mutation gained increased expression especially in the fruit pedicel through a 579-bp sequence deletion in its 5′upstream region,resulting in the phenotype of pendent fruit.The function of Up was confirmed by virus-induced gene silencing.Taken together,these findings constitute a cornerstone for understanding the domestication and differentiation of a key horticultural crop. | Yacong Cao Kang Zhang Hailong Yu Shumin Chen Donghui Xu Hong Zhao Zhenghai Zhang Yinqing Yang Xiaozhen Gu Xinyan Liu Haiping Wang Yaxin Jing Yajie Mei Xiang Wang Véronique Lefebvre Weili Zhang Yuan Jin Dongliang An Risheng Wang Paul Bosland Xixiang Li IIan Paran Baoxi Zhang Giovanni Giuliano Lihao Wang Feng Cheng | 2022 | Molecular Plant2022，15，11： | 0 |
| 13 | Investigation on step overcharge to self-heating behavior and mechanism analysis of lithium ion batteries显示文摘To obtain intrinsic overcharge boundary and investigate overcharge mechanism,here we propose an innovative method,the step overcharge test,to reduce the thermal crossover and distinguish the overcharge thermal behavior,including 5%state of charge(SOC)with small current overcharge and resting until the temperature equilibrium under adiabatic conditions.The intrinsic thermal response and the self-excitation behaviour are analysed through temperature and voltage changes during the step overcharge period.Experimental results show that the deintercalated state of the cathode is highly correlated to self-heating parasitic reactions.Before reaching the upper limit of Negative/Positive(N/P)ratio,the temperature changes little,the heat generation is significantly induced by the reversible heat(endothermic)and ohmic heat,which could balance each other.Following that the lithium metal is gradually deposited on the surface of the anode and reacts with electrolyte upon overcharge,inducing selfheating side reaction.However,this spontaneous thermal reaction could be“self-extinguished”.When the lithium in cathode is completely deintercalated,the boundary point of overcharge is about 4.7 V(~148%SOC,>40℃),and from this point,the self-heating behaviour could be continuously triggered until thermal runaway(TR)without additional overcharge.The whole static and spontaneous process lasts for 115 h and the side reaction heat is beyond 320,000 J.The continuous self-excitation behavior inside the battery is attributed to the interaction between the highly oxidized cathode and the solvent,which leads to the dissolution of metal ions.The dissolved metal ions destroy the SEI(solid electrolyte interphase)film on the surface of the deposited Li of anode,which induces the thermal reaction between lithium metal and the solvent.The interaction between cathode,the deposited Li of anode,and solvent promotes the temperature of the battery to rise slowly.When the temperature of the battery reaches more than 60℃,the reaction between lithium metal and solvent is accelerated.After the temperature rises rapidly to the melting point of the separator,it triggers the thermal runaway of the battery due to the short circuit of the battery. | Fengling Yun Shiyang Liu Min Gao Xuanxuan Bi Weijia Zhao Zenghua Chang Minjuan Yuan Jingjing Li Xueling Shen Xiaopeng Qi Ling Tang Yi Cui Yanyan Fang Lihao Guo Shangqian Zhao Xiangjun Zhang Shigang Lu | 2023 | Journal of Energy Chemistry2023，，4： | 0 |
| 14 | Dispersed multiphase flows:advances in measuring,simulation and modeling显示文摘Dispersed multiphase flows are commonly encountered in a wide variety of natural and industrial applications,from sedimentation in rivers and plankton surfacing in oceanic flows to fluidized bed in chemical reactors and pneumatic conveying systems.In these flows,a dispersed phase(con-sisting of particles,droplets and/or bubbles)is transported by a carrier phase.The interaction between the phases re-sults in complex dynamics(e.g.,transport,spatial clustering,and deformation or break-up)that is rich in physics and poses a wide spectrum of theoretical,computational,and experimental challenges.These challenges are at the very core of the research efforts made by the multiphase flow community over the past decades. | Cristian Marchioli Lihao Zhao | 2022 | Acta Mechanica Sinica2022，38，8： | 0 |
| 15 | Swimming strategy of settling elongated micro-swimmers by reinforcement learning显示文摘Particular types of plankton in aquatic ecosystems can coordinate their motion depending on the local flow environment to reach regions conducive to their growth or reproduction.Investigating their swimming strategies with regard to the local environment is important to obtain in-depth understanding of their behavior in the aquatic environment.In the present research,to examine an impact of the shape and gravity on a swimming strategy,plankton is considered as settling swimming particles of ellipsoidal shape.The Q-learning approach is adopted to obtain swimming strategies for smart particles with a goal of efficiently moving upwards in a two-dimensional steady flow.Strategies obtained from reinforcement learning are compared to those of naive gyrotactic particles that are modeled considering the behavior of realistic plankton.It is found that the elongation of particles improves the performance of upward swimming by facilitating particles’resistance to the perturbation of vortex.In the case when the settling velocity is included,the strategy obtained by reinforcement learning has similar performance to that of the naive gyrotactic one,and they both align swimmers in upward direction.The similarity between the strategy obtained from machine learning and the biological gyrotactic strategy indicates the relationship between the aspherical shape and settling effect of realistic plankton and their gyrotactic feature. | JingRan Qiu WeiXi Huang ChunXiao Xu LiHao Zhao | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，8： | 0 |
| 16 | Statistics of Particle Suspensions in Turbulent Channel Flow显示文摘Particle dynamics in a turbulent channel flow is considered.The effects of particle concentration and Reynolds number on the particle velocity statistics are investigated.Four different particle response times,τ^(+)=1,5,30 and 100,are examined for three different Reynolds numbers,Re_(∗)=200,360 and 790(based on channel height and friction velocity).The particle concentration evolves with time and statistics obtained during three different sampling periods might be distinctly different.Themean and fluctuating particle velocities are substantially affected both by the particle response time and by the Reynolds number of the flow. | Lihao Zhao Helge I.Andersson | 2012 | Communications in Computational Physics2012，11，4： | 0 |
| 17 | Corrigendum to‘Fabrication of a bio-instructive scaffold conferred with a favorable microenvironment allowing for superior implant osseointegration and accelerated in situ vascularized bone regeneration via type H vessel formation’[Bioactive Materials,Volume 9(March 2022)Page 491-507]显示文摘The authors regret a mistake of funding numbers in the Acknowledgment Section failed to be corrected during proofreading.Below is the corrected funding statement in ACKNOWLEDGMENT SECTION:This work was supported by the National Natural Science Foundation of China(NSFC)(Nos.82072415,81772354,81902189),Clinical Innovation Research Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory(2018GZR0201002),Science Technology Project of Guangzhou City(2019ZD15). | Yijun He Wenhao Wang Shaozhang Lin Yixi Yang Lizhi Song Yihan Jing Lihao Chen Zaopeng He Wei Li Ao Xiong Kelvin W.K.Yeung Qi Zhao Yuan Jiang Zijie Li Guoxian Pei Zhi-Yong Zhang | 2023 | Bioactive Materials2023，，2： | 0 |
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