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| 1 | Dysregulation of the miR-146a-Smad4 axis impairs osteogenesis of bone mesenchymal stem cells under inflammation显示文摘Osteoporosis is a common disease that affects patient quality of life, especially among the elderly population.Although inflammation contributes significantly to osteoporosis, the underlying mechanism is unclear. In this study, we found that tumor necrosis factor(TNF)-α, an inflammatory environment mimic, inhibits osteogenesis of bone mesenchymal stem cells(BMSCs), induces mi R-146 a and decreases Smad4. Moreover,overexpression of mi R-146 a inhibited the osteogenic ability of BMSCs, whereas blocking mi R-146 a partially rescued the osteogenesis deficiency under TNF-α treatment. Molecularly, mi R-146 a decreased Smad4 expression at the protein level by binding to an element located in the Smad4 30-untranslated region, and restoration of Smad4 reversed the inhibitory effects of mi R-146 a on osteogenesis. Together, our results showed that the inflammatory environment mimic TNF-α inhibits osteogenesis via upregulation of mi R-146 a and subsequent downregulation of Smad4, thus suggesting that therapeutic manipulation of mi R-146 a maybe a potential strategy to improve osteogenesis in the context of osteoporosis. | Wei Kuang Liwei Zheng Xin Xu Yao Lin Jiong Lin Jiahua Wu Jiali Tan | 2017 | Bone Research2017，5，4： | 13 |
| 2 | Gold nanorods together with HSP inhibitor-VER-155008 micelles for colon cancer mild-temperature photothermal therapy显示文摘Enhancing the heat-sensitivity of tumor cells provides an alternative solution to maintaining the therapeutic outcome of photothermal therapy(PTT). In this study, we constructed a therapeutic system, which was composed of methoxy-polyethylene-glycol-coated-gold-nanorods(MPEG-AuN R) and VER-155008-micelles, to evaluate the effect of VER-155008 on the sensitivity of tumor cells to heat, and further investigate the therapeutic outcome of MPEG-AuN R mediated PTT combined with VER-155008-micelles. VER-155008-micelles downregulate the expression of heat shock proteins and attenuate the heat-resistance of tumor cell. The survival of HCT116 cells treated with VER-155008-micelles under 45 ℃ is equal to that treated with high temperature hyperthermia(55 ℃) in vitro. Furthermore, we proved either the MPEG-AuN R or VER-155008-micelles can be accumulate in the tumor site by photoacoustic imaging and fluorescent imaging. In vivo anti-cancer evaluation showed that tumor size remarkably decreased(smaller than 100 mm^3 or vanished) when treated with combing 45℃ mild PTT system, which contrasted to the tumor size when treated with individual 45℃ mild PTT(around 500nm^3) or normal saline as control(larger than 2000 nm^3). These results proved that the VER-155008-micelles can attenuate the heat-resistance of tumor cells and enhance the therapeutic outcome of mild-temperature photothermal therapy. | Xichuan Tang Liwei Tan Kun Shi Jinrong Peng Yao Xiao Wenting Li Lijuan Chen Qian Yang Zhiyong Qian | 2018 | Acta Pharmaceutica Sinica B2018，8，4： | 12 |
| 3 | Metal-Organic Framework Nanocarriers for Drug Delivery in Biomedical Applications显示文摘Investigation of metal–organic frameworks(MOFs)for biomedical applications has attracted much attention in recent years.MOFs are regarded as a promising class of nanocarriers for drug delivery owing to well-defined structure,ultrahigh surface area and porosity,tunable pore size,and easy chemical functionalization.In this review,the unique properties of MOFs and their advantages as nanocarriers for drug delivery in biomedical applications were discussed in the first section.Then,state-ofthe-art strategies to functionalize MOFs with therapeutic agents were summarized,including surface adsorption,pore encapsulation,covalent binding,and functional molecules as building blocks.In the third section,the most recent biological applications of MOFs for intracellular delivery of drugs,proteins,and nucleic acids,especially aptamers,were presented.Finally,challenges and prospects were comprehensively discussed to provide context for future development of MOFs as efficient drug delivery systems. | Yujia Sun Liwei Zheng Yu Yang Xu Qian Ting Fu Xiaowei Li Zunyi Yang He Yan Cheng Cui Weihong Tan | 2020 | Nano-Micro Letters2020，12，8： | 10 |
| 4 | High-Performance Low-Temperature Transparent Conducting Aluminum- Doped ZnO 1lain Films and Apphcations 显示文摘 | Hao Xiaotao Tan Liwei Ong Kiansoo | 2006 | Journal of Crystal Growth2006，287，： | 1 |
| 5 | Synthesis, characterization, and flocculation performance of anionic polyacrylamide P (AM‐AA‐AMPS)显示文摘 | Jiangya Ma Huaili Zheng Mingzhuo Tan Liwei Liu Wei Chen Qingqing Guan Xiaokai Zheng | 2013 | J. Appl. Polym. Sci2013，，4： | 1 |
| 6 | Effect of annealing on microstructure evolution and mechanical property of cold forged magnesium pipes显示文摘 | Liwei Lu Tianmo Liu Ming-Jen Tan Jian Chen Zhongchang Wang | 2012 | Materials and Design2012，，： | 1 |
| 7 | A two-stage scheduling optimization model and solution algorithm for wind power and energy storage system considering uncertainty and demand response显示文摘 | Tan Zhongfu Ju Liwei Li Huanhuan | 2014 | International Journal of Electrical Power and Energy Systems2014，，63： | 1 |
| 8 | Conjugated copolymers based on fluorene-thieno thiophene for light-emitting diodes and photovoltaic cells显示文摘 | Tang Weihua Ke Lin Tan Liwei | 2007 | Macromolecules2007，40，17： | 1 |
| 9 | Optimization of fermentation process of papaya sauerkraut using response surface method显示文摘Papaya,a tropical fruit was used as the raw material to produce sauerkraut in the study.Three lactic acid bacteria strains isolated from papaya were added to the sauerkraut to facilitate the fermentation of papaya sauerkraut.In the fermentation process,the dynamic changes of total acid in sauerkrauts at different levels of sugar concentration,salt concentration,inoculation and temperature were studied.The response surface method was used to study the effects of changes in multiple factors at the same time.On the basis of“one-variable-at-a-time”approach,the response surface method optimized papaya sauerkraut fermentation process.According to the change of total acid in single factor,29 experiments were designed by 4×3 factorial central composite design.The optimum fermentation conditions were obtained as follows:sugar at 3.8%,salt at 2.8%,inoculation at 5%,and temperature at 31℃. | Zhang Lijuan Yang Jinsong Ma Liwei Tan Haisheng | 2014 | International Journal of Agricultural and Biological Engineering2014，7，3： | 1 |
| 10 | 槲皮素对人肺癌细胞株A549化疗药物增敏作用的实验研究(英文)显示文摘Objective: The present study was designed to investigate whether quercetin exerts increasing chemosensitivity on human lung adenocarcinoma cells when quercetin combined with cisplatin (DDP) and vincristine (VCR) in vitro respectively and its possible antitumor mechanism. To provide experimental proof for clinical combination application. Methods: Using intermittent administration of high dose VCR, human lung adenocarcinoma sensitive cell line (A549/S) was induced to VCR-resistant human lung adenocarcinoma cell line (A549/VCR). MTT assay was adapted for examing the 50% inhibition (IC50) value of DDP and VCR on A549/S and A549/VCR when quercetin combined with DDP and VCR respectively. Results: IC50 of DDP on A549/S and A549/VCR was 10.18 and 12.35 mg/L, and the IC50 of VCR on the two cell lines was 1.21 and 12.77 mg/L, respectively. The resistance fold of A549/VCR on VCR and DDP was 10.55 and 1.21, respectively. When quercetin at concentration of 50, 100 and 200 μmol/L in combination with DDP and VCR respectively, the IC50 of DDP and VCR on A549/S and A549/VCR were obvious decreased (P < 0.05 - P < 0.01). Conclusion: The experiment results suggested that quercetin could increase the chemosensitivity and partly revise the resistance of A549/VCR. | Xuejun Zhan Runxiang Zhang Yanping Xu Shuhua Yang Daze Xie Liwei Tan | 2012 | The Chinese-German Journal of Clinical Oncology2012，11，7： | 1 |
| 11 | Ceiling-based vis-ual positioning for an indoor mobile robot with monocularvision 显示文摘 | XU De HAN Liwei TAN Min | 2009 | IEEE Transactions on Industrial Electronics2009，56，5： | 1 |
| 12 | Suppression of LjBAK1-mediated immunity by SymRK promotes rhizobial infection in Lotus japonicus.显示文摘An important question in biology is how organisms can associate with different microbes that pose no threat (commensals), pose a severe threat (pathogens) and those that are beneficial (symbionts). The root nodule symbiosis serves as important model system to address such questions in the context of plant-microbe interactions. It is now generally accepted that rhizobia have the abilities to actively suppress host immune responses during the infection process, analogous to the way in which plant pathogens can evade immune recognition. However, much remains to be elucidated with regard to the mechanisms by which the host recognizes the rhizobia as pathogens and how, subsequently, these pathways are suppressed to allow establishment of the nitrogen fixing symbiosis. In this study, we found that SymRK (Symbiosis Receptor-like Kinase) is required for rhizobial suppression of plant innate immunity in Lotus japonicus. SymRK associates with LjBAK1 (BRASSINOSTEROID INSENSITIVE 1-Associated receptor Kinase 1), a well characterized, positive regulator of plant innate immunity, and directly inhibits LjBAK1 kinase activity. Rhizobial inoculation enhances the association between SymRK and LjBAK1 in planta. LjBAK1 is required to regulate plant innate immunity and plays a negative role in mediating rhizobial infection in L. japonicus. The data indicate that the protein complex of SymRK-LjBAK1 serves as an intersection point between rhizobial symbiotic signaling pathways and innate immunity pathways, which provides an evidence that rhizobia might actively suppress the host's ability to mount a defense response in the legume-rhizobium symbiosis. | Yong Feng Ping Wu Chao Liu Liwei Peng Tao Wang Chao Wang Qian Tan Bixuan Li Yajuan Ou Hui Zhu Songli Yuan Renliang Huang Gary Stacey Zhongming Zhang Yangrong Cao | 2021 | Molecular Plant2021，14，11： | 0 |
| 13 | Discovery of 4-cyclopropyl-3-(2-((1-cyclopropyl-1H-pyrazol-4-yl) amino) quinazolin-6-yl)-N-(3-(trifluoromethyl) phenyl) benzamides as potent discoidin domain receptor inhibitors for the treatment of idiopathic pulmonary fibrosis显示文摘Idiopathic pulmonary fibrosis(IPF)is a chronic fatal lung disease with a median survival time of 3–5 years.Inaccurate diagnosis,limited clinical therapy and high mortality together indicate that the development of effective therapeutics for IPF is an urgent need.In recent years,it was reported that DDRs are potential targets in anti-fibrosis treatment.Based on previous work we carried out further structure modifications and led to a more selective inhibitor 47 by averting some fibrosis-unrelated kinases,such as RET,AXL and ALK.Extensive profiling of compound 47 has demonstrated that it has potent DDR1/2 inhibitory activities,low toxicity,good pharmacokinetic properties and reliable in vivo anti-fibrosis efficacy.Therefore,we confirmed that discoidin domain receptors are promising drug targets for IPF,and compound 47 would be a promising candidate for further drug development. | Qi Wang Bixi Tang Dandan Sun Ying Dong Yinchun Ji Huanyu Shi Liwei Zhou Yueyue Yang Menglan Luo Qian Tan Lin Chen Yue Dong Cong Li Rongrong Xie Yi Zang Jingkang Shen Bing Xiong Jia Li Danqi Chen | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 0 |
| 14 | Design and implementation of international agricultural and biological engineering expert management system based on WEB mode显示文摘Agriculture and biological engineering are the foundation of agricultural modernization,and related countries have also issued relevant policies to guide the development of agriculture and biotechnology.Agricultural and biological engineering experts are the intellectual resources in the field.At present,there is less research on the management and maintenance of agricultural and biological engineering experts,and there is a lack of software systems in this area.In order to realize the management and maintenance of agricultural and biological engineering expert information,a service platform for the international agricultural and biological engineering expert system based on the B/S framework has been developed.The background of the system used C#as the development language,and the foreground used JavaScript technology and Bootstrap.The software adopted ASP.NET MVC as the web development framework and used the Entity Framework to operate the SQL Server background database.The system has the function of searching and querying agricultural and biological engineering expert information according to keywords,and implements the functions of adding,deleting,and modifying data records,and the function of generating spreadsheets and importing spreadsheet data.The development of this system provides effective management tools for the maintenance and construction of agricultural and biological engineering expert databases and lays a good foundation for the construction of agricultural and biological engineering think tanks. | Jianbo Shen Liwei Tan Yingkuan Wang | 2020 | International Journal of Agricultural and Biological Engineering2020，13，6： | 0 |
| 15 | Effects of increased human tumor necrosis factor-like molecule 1A expression in peripheral blood of children with acute Guillain-Barre syndrome on interferon-gamma secretion显示文摘BACKGROUND:Human tumor necrosis factor-like molecule 1A(hTL1A) is a strong T helper cell type 1(Th1) co-stimulator.Guillain-Barre syndrome(GBS) is an autoimmune disorder of the nervous system,which is mediated by Th1 cells. OBJECTIVE:To determine hTL1A expression in peripheral blood T lymphocytes of acute GBS children and the effects of hTL1 A on secretion of interferon-γ. DESIGN,TIME AND SETTING:A randomized,controlled,neuroimmunological in vitro study was performed at the Central Laboratory of First Hospital of Jilin University,China from November 2005 to November 2007. MATERIALS:Venous blood samples were obtained from 6 healthy donors,aged 6-12 years(all routine blood examination items were normal),and 6 additional children with acute GBS,aged 6-12 years.The GBS children fell ill within 1 week and were not treated with hormones or immunoglobulin. Purified recombinant human soluble tumor necrosis factor-like molecule 1A(rhsTL1A,1 mg/mL, relative molecular mass 22 000,6×His tag,soluble form) was supplied by the Central Laboratory of First Hospital of Jilin University,China. METHODS:Peripheral blood mononuclear cells were isolated from healthy donors using the standard Ficoll gradient centrifugation and were incubated in 96-well culture plates.The cells were assigned to the following groups:control(2μg/mL phytohemagglutinin),2μg/mL phytohemagglutinin + 25,100 and 400 ng/mL rhsTL1A.T cell proliferation was quantified using the tritiated thymidine(~3H-TdR) method.Serum interferon-γlevels in acute GBS children were detected by enzyme-linked immunosorbent assay(ELISA).The ratio of hTL1 A-positive T cells to CD3-positive T cells in peripheral blood of acute GBS children was determined using flow cytometry. Following in vitro pre-activation of peripheral blood mononuclear cells by 2μg/mL phytohemagglutinin,the peripheral blood mononuclear cells were treated with 400 ng/mL exogenous rhsTL1A.Finally,peripheral blood mononuclear cell-secreted interferon-γlevels were measured by ELISA. MAIN OUTCOME MEASURES:The following parameters were measured:rhsTL1A stimulation index to stimulate proliferation of T cells;the serum interferon-γlevels in acute GBS children;the ratio of hTL1A-positive cells to CD3-positive cells;the levels of interferon-γsecreted by peripheral blood mononuclear cells in acute GBS children,as well as rhsTL1A-stimulated interferon-γlevels. RESULTS:T cell proliferation assay revealed that the stimulation index in each rhsTL1A group was greater than the control group.The stimulation index of the 400 ng/mL rhsTL1A group was the greatest.Serum interferon-γlevels in acute GBS children were significantly greater than the control group(P<0.05).The ratio of hTL1 A^+ CD3^+ T cells to CD3^+ T cells in acute GBS children was significantly greater than the control group(P<0.01).Phytohemagglutinin stimulated peripheral blood mononuclear cells to a greater extent than 400 ng/mL rhsTL1A in the acute GBS group,and the secreted interferon-γlevels were significantly increased(P<0.05). CONCLUSION:In T cells pre-activated with 2μg/mL phytohemagglutinin,proliferation was effectively increased with 400 ng/mL rhsTL1A treatment.Expression of hTL1 A was increased in activated T cells from peripheral blood of acute GBS children,followed by increased interferon-γsecretion.These mechanisms are considered to be part of the pathological process that induces the secretion of inflammatory cytokines in GBS syndrome. | Libin Yang Shulei Li Yan Tan Shufen Xu Xiumei Duan Yanqiu Fang Lihua Liu Yuanyuan Che Lei Liu Liwei Zhou | 2009 | Neural Regeneration Research2009，4，10： | 0 |
| 16 | Generation mechanism of NO_(x)and N_(2)O precursors(NH_(3)and HCN)from aspartic acid pyrolysis:A DFT study显示文摘In order to better understand the mechanism of NO_(x)and N_(2)Oprecursors(NH_(3)and HCN)from aspartic acid(Asp)pyrolysis,decomposition reaction networks resulting in the generation of NH_(3)and HCN were investigated by employing density function theory methods.After several pathways were analyzed in detail,two series of pyrolytic reactions containing three possible pathways were proposed.All the reactants,transition states,intermediates and products were optimized,also the electronic properties on these crucial points were discussed,which shows that Cαacts as the most active site to initiate the pyrolysis reaction,where the direct Cα-Cβbond breakage,due to the atomic charge population of repulsion,led to one key route for the generation of HCN,and the transfer of Hαfrom Cαto Cβresulting in another key route for the generation of HCN,while the transfer of Hαfrom Cαto N atom of Asp resulting in the key route for the generation of HN3.Further,the kinetic analysis based on speed control method in each key reaction pathway was conducted to further compare the generation of HCN and NH_(3)under various temperatures.The above results are in accordance with the related experimental results. | Kang Peng Qin Wu Fu Zongqiang Wang Tipeng Ju Liwei Tan Zhongfu | 2016 | International Journal of Agricultural and Biological Engineering2016，9，5： | 0 |
| 17 | Analyzing the formation cause of Xidatan drinking mineral springs in island permafrost area on north slope of the Kunlun Mountains显示文摘The replenishment source of Xidatan drinking mineral springs in island permafrost area on north slope of the Kunlun Mountains are mainly the melting water from the modern glaciers bottom, snow and ice melting water, atmospheric precipitation, and surface water in Yuzhu Peak area on the Kunlun Mountains. This scenario is based on the survey of hydrogeology, water-conducting and water-controlling faults, and water chemistry, and on the EH-4 high-frequency electronic deep exploration. The original water recharges the deep groundwater at fracture zone of active normal faults F3 and F4 , then groundwater enriches at normal faults F2 and F2-1,2 , and then run northward. A water-rich triangle area is formed when groundwater reach the active reverse fault F1 . Groundwater then discharges through fracture zone of F1 , which is the major cause of the Xidatan mineral springs formation. | SenQi Zhang JiJiao Fan HongTao Wu Fan Jiang LiWei Tan | 2010 | Research in Cold and Arid Regions2010，2，3： | 0 |
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