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| 1 | Antioxidant effect of apolipoprotein A-I on high-fat diet-induced non-alcoholic fatty liver disease in rabbits显示文摘非酒精的脂肪肝疾病(NAFLD ) 是包含肝畸形的一个系列的一个逐渐地公认的条件。氧化压力和类脂化合物 peroxidation 是在 NAFLD 的关键 pathophysiological 机制,这被建议了。尽管 apolipoprotein A-I 的抗氧化剂效果(apoA -- 我) 被报导了,它对 NAFLD 的影响没被报导。这研究的目的是决定是否 apoA -- 我能改进在兔子通过它的抗氧化剂行动与高脂肪的导致食谱的 NAFLD 联系的生物化学、组织学的畸形。肝损坏被肝的系数评估,肝的类脂化合物试金,肝象肝节染色的 hematoxylineosin 一样的明显的畸形。类脂化合物 peroxidation 被测量 malondialdehyde (MDA ) 估计在肝的水平。氧化应力被测量 superoxide 估计 dismutase (草皮) ,谷胱甘肽 peroxidase (GPx ) ,和可诱导的氮的氧化物 synthase (iNOS ) 在浆液和肝的活动。另外,草皮, GPx,和过氧化氢酶(猫) 的 mRNA 表情层次被即时量的聚合酶链反应方法决定。结果显示出那 apoA --(20 或 40 mg/kg/w ) 我在减少全部的胆固醇, triglyceride,低密度的脂蛋白胆固醇,和 MDA 在高脂肪的食谱兔子铺平的肝的脂肪变性,发炎,肝的系数,和肝是有效的。另外, apoA -- 当在浆液和肝减少 iNOS 活动时,我增加了草皮和 GPx 活动。而且, apoA -- 我显著地在肝增加了草皮, GPx,和猫的 mRNA 表示层次。这研究显示出那 apoA -- 我在一本高脂肪的食谱导致的兔子对脂肪肝疾病施加了保护的效果,可能通过它的抗氧化剂行动。 | Weina Wang Wei Zhou Baolong Wang Haiyan Zhu Li Ye Meiqing Feng | 2013 | Acta Biochimica et Biophysica Sinica2013，45，2： | 5 |
| 2 | Human apolipoprotein A-I exerts a prophylactic effect on high-fat diet-induced atherosclerosis via inflammation inhibition in a rabbit model显示文摘Apolipoprotein A-I (apoA -- 我) 是高密度的脂蛋白的主要功能的蛋白质部分。人的 apoA 的预防效果和机制 -- 我在动脉粥样硬化上(作为) 被调查在一高脂肪象兔子模型导致食谱。兔子与 apoA 被注射 -- 我每周一次当喂时为 20 个星期的高脂肪的饮食。我们的结果显示出那 apoA -- 我能提起高密度的脂蛋白胆固醇的浆液水平并且类脂化合物总数胆固醇, triglyceride,和低密度的脂蛋白胆固醇减少那些在作为兔子。减少的大动脉的匾区域和大动脉的损害度被染色的油红 O 也观察并且他在 apoA-I-treated 染色高脂肪象兔子导致食谱。进一步的学习阐明了那 apoA -- 我能包括细胞间的粘附分子的一些煽动性的调停人的表示打的下面调整 1,脉管的粘附 molecule-1 (VCAM-1 ) ,单核白血球 chemoattractant protein-1,肿瘤坏死因素 -- , interleukin-6 (IL-6 ) ,和在浆液和主动脉的 C 反应的蛋白质作为兔子。另外,即时量的 RT-PCR 分析证明 apoA --我注入减少了二个 支持inflammatory 分子的 mRNA 层次,即原子因素 kappa B ( NF-B )和 cyclooxygenase-2 ( COX-2 ),在主动脉作为兔子,它在 endothelial VCAM-1 和 IL-6 mRNA 抄写与伴随物减小被联系。一起,我们的结果支持 atheroprotective 和 apoA 的预防角色 -- 我可以与它的反煽动性的效果在 vivo,和这项活动被相关。 | Jiyang Li Weina Wang Lei Han Meiqing Feng Hui Lu Li Yang Xiangxiang Hu Si Shi Shanshan Jiang Qian Wang Li Ye | 2017 | Acta Biochimica et Biophysica Sinica2017，49，2： | 3 |
| 3 | Inhibitory effect of apolipoprotein A-I on matrix metalloproteinase-2 expression in vivo and in vitro显示文摘在现在的学习,我们调查了 apolipoprotein A-I 的效果(apoA -- 我) 在矩阵 metalloproteinase-2 (MMP-2 ) 上在 vivo 并且在 vitro 的表示。首先,我们检测了 apoA 的效果 -- 我在主动脉 MMP-2 上,激活 proliferator 的受体 /(PPAR /) , cyclooxygenase-2 (COX-2 ) ,和在动脉粥样硬化患者兔子的原子因素 kappa B (NF-B ) 表情用 immunohistochemical 方法建模的 peroxisome。结果证明 MMP-2, COX-2,和 NF-B 的表情在与 apoA 对待的动脉粥样硬化患者兔子的主动脉被减少 -- 我,当 PPAR / 表示被增加时。然后,我们选择了重要发炎房间,在 vitro 证明那些效果的巨噬细胞。巨噬细胞被划分成六个组并且与 apoA 的不同集中对待 -- 我, MMP-2 的 mRNA 表情, PPAR / ,和 COX-2 然后用 PPAR 的反向抄写的聚合酶链反应,和蛋白质表示被决定, NF-B 被西方的污点分析检测。在有教养的上层清液的 MMP-2 和 PPAR 的层次用连接酶的 immunosorbent 试金被决定。有趣地,在里面 vitro 结果类似于结果在里面 vivo 学习。在有 apoA 的孵化以后 -- 我为 24 h, MMP-2 的表情, COX-2,和 NF-B 被减少,当 PPAR / 表示被增加时。在他们在在 vivo 并且在 vitro 做匾马厩的进程的特别角色的考虑,我们推测 apoA 的禁止的效果 -- 我可以与 apoA 的效果在 MMP-2 表示上有一种靠近的关系 -- 我在 PPAR / , COX-2,和 NF-B 表达式上。尽管进一步的研究被需要澄清这些效果的内在的机制,我们的调查结果提供新奇卓见进 apoA 的反动脉粥样硬化患者匾破裂效果 -- 我。 | Weina Wang Xunlong Shi Yuan Yuan Haiyan Zhu Wei Zhou Hai Huang Meiqing Feng | 2013 | Acta Biochimica et Biophysica Sinica2013，45，3： | 2 |
| 4 | Biotransformation pathways of ginsenoside Rb1 to compound K by β-glucosidases in fungus Paecilomyces Bainier sp. 229显示文摘 | Qin Yan Wei Zhou XunLong Shi Pei Zhou DianWen Ju MeiQing Feng | 2010 | Process Biochemistry2010，，9： | 1 |
| 5 | In vitro and in vivo anti hepatitis B virus activitiies of a plant extract from Geranium carolinia- hum L显示文摘 | Jiyang Li Hai Huang Meiqing Feng et a| | 2009 | Antiviral Research2009，79，： | 1 |
| 6 | The experimental study on the differentiation of neural stem cells into retinal ganglion cells 显示文摘 | FENG Dong-fu LU Yi-cheng LOU Meiqing | 2006 | J Neurotrauma2006，23，6： | 1 |
| 7 | A novel class of potent influenza virus inhibitors:Polysubstituted acylthiourea and its fused heterocycle derivatives显示文摘 | Sun Chuanwen Huang Hai Feng Meiqing | 2006 | Bioorg Med Chem Lett2006，16，1： | 1 |
| 8 | In vitro and in vivo anti-hepatitis B virus activities of a plant extract from Geranium carolinianum L显示文摘 | Jiyang Li Hai Huang Meiqing Feng | 2008 | Antiviral Research2008，79，： | 1 |
| 9 | Studies on the preparation, crystal structure and bioactivity of ginsenoside compound K显示文摘 | ZHOU Wei FENG Meiqing LI Jiyang | 2006 | J Asian Nat Prod Res2006，8，6： | 1 |
| 10 | A reporter gene assay for determining the biological activity of therapeutic antibodies targeting TIGIT显示文摘T cell immunoglobulin and ITIM domain(TIGIT)is a novel immune checkpoint that has been considered as a target in cancer immunotherapy.Current available bioassays for measuring the biological activity of therapeutic antibodies targeting TIGIT are restricted to mechanistic investigations because donor primary T cells are highly variable.Here,we designed a reporter gene assay comprising two cell lines,namely,CHO-CD112-CD3 scFv,which stably expresses CD 112(PVRL2,nectin-2)and a membranebound anti-CD3 single-chain fragment variable(scFv)as the target cell,and Jurkat-NFAT-TIGIT,which stably expresses TIGIT as well as the nuclear factor of activated T-cells(NFAT)response element-controlled luciferase gene,as the effector cell.The anti-CD3 scFv situated on the target cells activates Jurkat-NFATTIGIT cells through binding and crosslinking CD3 molecules of the effector cell,whereas interactions between CD 112 and TIGIT prevent activation.The presence of anti-TIGIT mAbs disrupts their interaction,which in turn reverses the inactivation and luciferase expression.Optimization and validation studies have demonstrated that this assay is superior in terms of specificity,accuracy,linearity,and precision.In summary,this reliable and effective reporter gene assay may potentially be utilized in lot release control,stability assays,screening,and development of novel TIGIT-targeted therapeutic antibodies. | Zhihao Fu Hongchuan Liu Lan Wang Chuanfei Yu Yalan Yang Meiqing Feng Junzhi Wang | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 1 |
| 11 | Chemically triggered life control of“smart”hydrogels through click and declick reactions显示文摘The degradation of polymeric materials is recognized as one of the goals to be fulfilled for the sustainable economy.In this study,a novel methodology was presented to synthesize multiple highly cross-linked polymers(i.e.,hydrogels)through amine–thiol scrambling under mild conditions.Amine-terminated poly(ethylene glycol)(PEGNH_(2))was reacted with the representative conjugate acceptors to synthesize hydrogels in organic and aqueous solutions,respectively.The materials above exhibited high water-swelling properties,distributed porous structures,as well as prominent mechanical strengths.It is noteworthy that the mentioned hydrogels could be degraded efficiently in hours to release the original coupling partner,which were induced by ethylene diamine at ambient temperature through amine-amine metathesis.The recovered PEG-NH2 reagent could be employed again to regenerate hydrogels.Due to the multiple architectures and functions in polymeric synthesis,degradation and regeneration,a new generation of“smart”materials is revealed. | Xing Feng Meiqing Du Hongbei Wei Xiaoxiao Ruan Tao Fu Jie Zhang Xiaolong Sun | 2022 | Frontiers of Chemical Science and Engineering2022，16，9： | 0 |
| 12 | Establishing a cat model of chronic optic nerve compression injury显示文摘BACKGROUND:An animal model of chronic optic nerve injury is necessary to further understand the pathological mechanisms involved. OBJECTIVE:To establish a stabilized,chronic,optic nerve crush model,which is similar to the clinical situation to explore histopathological and optic electrophysiological changes involved in this injury. DESIGN,TIME AND SETTING:A randomized and controlled animal trial was performed at Shanghai Institute of Neurosurgery from May to October 2004. MATERIALS:A BAL3XRAY undetachable balloon and Magic-BD catheter were provided by BLAT, France;JX-2000 biological signal processing system by Second Military Medical University of Chinese PLA,China;inverted phase contrast microscopy by Olympus,Japan. METHODS:A total of twenty normal adult cats were randomly assigned to control(n=5) and model (n=15) groups,according to different doses of contrast agent injected through balloons as follows: 0.2 mL injection,0.25 mL injection,and 0.35 mL injection,with each group containing 5 animals. Imitating the clinical pterion approach,the optic nerves were exposed using micro-surgical methods. An engorged undetachable balloon was implanted beneath the nerve and connected to a catheter. Balloon size was controlled with a contrast agent injection(0.1 mL/10 min) to form an occupying lesion model similar to sellar tumors. MAIN OUTCOME MEASURES:The visually evoked potential examination was used to study optical electrophysiology changes in pre-post chronic optical nerve injury.Ultrastructural pathological changes to the optic nerve were analyzed by electron microscopy. RESULTS:During the early period(day 11 after modeling),visually evoked potential demonstrated no significant changes.In the late period(day 51 after modeling),recorded VEP demonstrated that P1 wave latency was prolonged and P1 wave amplitude was obviously reduced.Following injury, the endoneurium,myelin sheath,lamella,axolemma,and axon appeared disordered. CONCLUSION:Results demonstrated that the chronic,intracranial,optical nerve crush model was stable and could simulate optic nerve lesions induced by sellar tumors.Under the condition of chronic optical nerve crush,visually evoked potentials were aggravated. | Feng Yu Shaoji Yuan Rongwei Zhang Yicheng Lu Meiqing Lou | 2009 | Neural Regeneration Research2009，4，6： | 0 |
| 13 | Identification of a hotspot on PD-L1 for pH-dependent binding by monoclonal antibodies for tumor therapy显示文摘Dear Editor,Monoclonal antibody(mAb)-based tumor immune checkpoint therapy(ICT)has gained particular interest in recent years.1 The molecular basis of binding between mAbs and PD-1 or PD-L1 has been reported,providing clear information of the binding“hotspots”for mAbs.2,3 Tumor suppression efficacy of PD-L1 specific mAbs relies on not only the blocking of PD-1/PD-L1 interaction to restore T cell reactivity,but also Fc-mediated tumor cell cytotoxity.PD-L1 antibody drug conjugate(ADC)for selective chemo-guided immune modulation of tumor has also been developed which has shown promising tumor suppression potency.4 MAbs that could bind to antigen in a pH-dependent manner would improve recycling of the antibodies and engineered IL-6R mAbs with pH-dependent binding properties have displayed increased lysosomal delivery and therapeutic potency.5 However,no PD-L1 specific mAb with pH-dependent binding property has been reported,and whether the binding to a specific region on PD-L1 would induce pH-dependent interaction remains unknown. | Hongchuan Liu Xiaoshan Bi Yuehua Zhou Rui Shi Sheng Yao Jianxun Qi Hui Feng Meiqing Feng Jinghua Yan Shuguang Tan | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 0 |
| 14 | Gelatin filter capture-based high-throughput sequencing analysis of microbial diversity in haze particulate matter显示文摘Airborne particulate matter(PM),especially PM2.5,can be easily adsorbed by human respiratory system.Their roles in carrying pathogens for spreading epidemic diseases has attracted great concern.Herein,we developed a novel gelatin filter-based and culture-independent method for investigation of the microbial diversity in PM samples during a haze episode in Tianjin,China.This method involves particle capture by gelatin filters,filter dissolution for DNA extraction,and high-throughput sequencing for analysis of the microbial diversity.A total of 584 operational taxonomic units(OTUs)of bacteria and 370 OTUs of fungi at the genus level were identified during hazy days.The results showed that both bacterial and fungal diversities could be evaluated by this method.This study provides a convenient strategy for investigation of microbial biodiversity in haze,facilitating accurate evaluation of airborne epidemic diseases. | Meiqing Sun Zhanlin Ding Hong Wang Guangping Yu Zhe Feng Bingzhi Li Penghui Li | 2019 | The Journal of Biomedical Research2019，33，6： | 0 |
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