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| 1 | Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma显示文摘AIM To establish the role of vascular endothelial growth factor (VEGF) in the oncogenesisof human gastric carcinoma more directly.METHODS The expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF levels were manipulated in human gastric cancer cell using eukaryotic expression constructs designed to express the complete VEGF165 complimentary DNA in either the sense or antisense orientation. The biological changes of the cells were observed in which VEGF was up-regulated or downregulated.RESULTS VEGF-positive rate was 50%, and VEGF was mainly localized in the cytoplasm and membrane of the tumor cells, while KDR was mainly located in the membrane of vascular endothelial cells in gastric cancer tissues and peri-cancerous tissue. In 2 cases of 50 specimens, the gastric cancer cells expressed KDR,localized in both the cytoplasm and membrane.Introduction of VEGF165 antisense into human gastric cancer cells ( SGC-7901, immunofluorescence intensity,31.6%)) resulted in a significant reduction in VEGFspecific messenger RNA and total and cell surface VEGF protein ( immunofluorescence intensity, 8.9%)(P<0.05). Conversely, stable integration of VEGF165 in the sense orientation resulted in an increase in cellular and cell surface VEGF (immunofluorescence intensity,75.4%) (P<0.05). Lowered VEGF levels were associated with a marked decrease in the growth of nude mouse xenografted tumor (at 33 days postimplantation, tomor volume: 345.40 ± 136.31 mm3) (P<0.05 vs control SGC7901 group: 1534.40 ± 362.88 mm3), whereas up-regulation of VEGF resulted in increased xenografted tumor size (at 33 days postimplantation, tomor volume: 2350.50 ± 637.70mm3) (P<0.05 vs control SGC-7901 group).CONCLUSION This study provides direct evidence that VEGF plays an important role in the oncogenesis of human gastric cancer. | Du-Hu Liu Xue-Yong Zhang Dai-Ming Fan Yu-Xin Huang Jin-Shan Zhang Wei-Quan Huang Yuan-Qiang Zhang Qing-Sheng Huang Wen-Yu Ma Yu-Bo Chai Ming Jin Institute of Digestive Disease,Xijing Hospital,~2 Department of Gastroenterology,Tangdu Hospital,~3Department of Histology and Embryology,~4 Department of Microbiology,~5 Department of Biochemistry,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 37 |
| 2 | Real world effectiveness of PCSK-9 inhibitors combined with statins versus statins-based therapy among patients with very high risk of atherosclerotic cardiovascular disease in China(RWE-PCSK study)显示文摘BACKGROUND The efficacy and safety of proprotein convertase subtilisin/kexin type 9(PCSK-9) inhibitors were confirmed by several clinical trials, but its effectiveness in routine clinical practice in China has not been evaluated. This study aims to describe the real world effectiveness of PCSK-9 inhibitors combined with statins compared with statins-based therapy among patients with very high risk of atherosclerotic cardiovascular disease(ASCVD).METHODS This is a multi-center observational study, enrolled patients from 32 hospitals who underwent percutaneous coronary intervention(PCI) from January to June in 2019. There are 453 patients treated with PCSK-9 inhibitors combined with statins in PCSK-9 inhibitor group and 2,610 patients treated with statins-based lipid lowering therapies in statins-based group. The lipid control rate and incidence of major adverse cardiovascular events(MACE) over six months were compared between two groups.A propensity score-matched(PSM) analysis was used to balance two groups on confounding factors. Survival analysis using Kaplan-Meier methods was applied for MACE.RESULTS In a total of 3,063 patients, 89.91% of patients had received moderate or high-intensity statins-based therapy before PCI, but only 9.47% of patients had low-density lipoprotein cholesterol(LDL-C) levels below 1.4 mmol/L at baseline. In the PSM selected patients, LDL-C level was reduced by 42.57% in PCSK-9 inhibitor group and 30.81%(P < 0.001) in statins-based group after six months. The proportion of LDL-C ≤ 1.0 mmol/L increased from 5.29% to 29.26% in PCSK-9 inhibitor group and 0.23% to 6.11% in statins-based group, and the proportion of LDL-C ≤ 1.4 mmol/L increased from 10.36% to 47.69% in PCSK-9 inhibitor group and 2.99% to 18.43% in statins-based group(P < 0.001 for both). There was no significant difference between PCSK-9 inhibitor and statins-based treatment in reducing the risk of MACE(hazard ratio = 2.52, 95% CI: 0.49-12.97, P = 0.250).CONCLUSIONS In the real world, PCSK-9 inhibitors combined with statins could significantly reduce LDL-C levels among patients with very high risk of ASCVD in China. The long-term clinical benefits for patients received PCSK-9 inhibitor to reduce the risk of MACE is still unclear and requires further study. | Yu-Qi LIU Dan-Dan LI Meng CHAI Hong-Liang CONG Xiao-Qiang CONG Jun DAI Rong-Pin DU Ming GAO Jin-Cheng GUO Yan-Qing GUO Xiao-Jian HONG Rong-Chong HUANG Feng-Shun JIA Jia-Yu LI Qing LI Jia-Mei LIU Xin-Ping LIU Yu-Guo LIU Hong-Gang NIE Bing SHAO Xiao-Yu SHEN Hai-Qing SONG Yi-Jun SONG Li-Jun WANG Shuo WANG Dong-Mei WU Jing XIA Zhi-Yong YANG Hong-Ying YU Hui ZHANG Tie-Mei ZHANG Ji-Yi ZHAO Liang-Chen ZHAO Ming-Qi ZHENG Yun-Dai CHEN | 2021 | Journal of Geriatric Cardiology2021，18，4： | 5 |
| 3 | Analysis of Gene Expression Pattern of Lumbar Intervertebral Disc Degeneration in Human显示文摘ObjectiveTo investigate the gene expression changes in normal and degeneration lumbar intervertebral disc in humans, providing information for clinical. MethodsThe PCR products of 4096 human genes were spotted onto a kind of chemical-material-coated-glass slides. The total RNAs were isolated from the tissues. Both the mRNAs from the degeneration and normal lumbar intervertebral disc in humans were reversely transcribed to the cDNAs, which used as the hybridization probes with the incorporations of fluorescent dUTP. The mixed probes were then hybridized to the cDNA microarray. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and analyzed with computer image analysis. ResultsAmong the 4096 targets, there were 706 genes whose expression levels differed between the degeneration and normal lumbar intervertebral disc in all cases, comprising 298 up-regulated and 358 down-regulated ones. ConclusionDNA microarray technology is an effective technique in screening for differently expressed genes between the degeneration and normal lumbar intervertebral disc. Cell apoptosis plays an important role in the process of lumbar intervertebral disc degeneration. | HU Ming MA Yuan-zheng FENG Hui-cheng CHEN Xing CHAI Xiao-jun PENG Wei LI Hong-wei | 2006 | 中国康复理论与实践2006，12，5： | 4 |
| 4 | Effect of growth and differentiation factor 6 on the tenogenic differentiation of bone marrow-derived mesenchymal stem cells显示文摘 | CHAI Wei NI Ming RUI Yun-feng ZHANG Kai-yi ZHANG Qiang XU Liang-liang CHAN Kai-ming LI Gang WANG Yan | 2013 | Chinese Medical Journal2013，，8： | 4 |
| 5 | Synthesis and in vitro antibacterial activities of 7-(4-alkoxyimino-3-hydroxypiperidin-1-y1)quinolone derivatives显示文摘A series of novel 7-(4-alkoxyimino-3-hydroxypiperidin-1-yl)quinolone derivatives were designed,synthesized and evaluated for in vitro antibacterial activities.Compounds 8f,8g,8i and 8j with the potencies similar to or better than those of levofloxacin and IMB against Staphylococcus aureus and Staphylococcus epidermidis,worth further investigation. | Wang, Ju Xian Guo, Qiang Chai, Yun Feng, Lian Shun Guo, Hui Yuan Liu, Ming Liang | 2010 | Chinese Chemical Letters2010，21，1： | 4 |
| 6 | Chinese clinical practice guidelines for ultrasound-guided irreversible electroporation of liver cancer(version 2022)显示文摘Introduction Liver cancer remains a global health challenge,and its incidence is increasing worldwide.It is estimated that by 2025,more than one million individuals will be affected by liver cancer annually[1,2].In recent years,ablation has become a widely accepted treatment option for patients with primary and secondary liver malignancies[3].The commonly used ablation method for liver cancer is thermal ablation,including radiofrequency ablation. | Min Xu Li-Ting Xie Yue-Yong Xiao Ping Liang Qi-Yu Zhao Zhong-Min Wang Wei-Lu Chai Ying-Tian Wei Lin-Feng Xu Xiao-Kun Hu Ming Kuang Li-Zhi Niu Chen-Guo Yao Hai-Ying Kong Guo Tian Xiao-Yan Xie Xin-Wu Cui Dong Xu Jun Zhao Tian-An Jiang The Interventional Diagnosis and Treatment Group of the Ultrasonic Medicine Branch of Chinese Medical Association,The Nanoknife Tumor Ablation Society of the Interventional Minimally Invasive Therapy Special Committee of China Medicine Education Association | 2022 | Hepatobiliary & Pancreatic Diseases International2022，21，5： | 2 |
| 7 | A survey of the development of quadruped robots: Joint configuration, dynamic locomotion control method and mobile manipulation approach显示文摘Some quadruped robots developed recently show better dynamic performance and environmental adaptability than ever, and have been preliminarily applied in the field of emergency disposal, military reconnaissance and infrastructure construction. The development route, mechanisms design, control methods and mobile manipulating approaches of the quadruped robots are surveyed in this article. Firstly, the development route of the quadruped robot is combed, as the references of the forecast of the future work on quadruped robots. Then the bionic structure and the motion control method of the quadruped robot is summarized, the advantages and disadvantages are analyzed in aspects of gait switching, terrain adaption and disturbance resistance. Subsequently, aiming at the mobile manipulation of the quadruped robot, the representative leg-arm collaborative robots and the multi-task-oriented Whole-body Control (WBC) methods are introduced. Finally, the summary and future work of the quadruped robots is given. | Hui Chai Yibin Li Rui Song Guoteng Zhang Qin Zhang Song Liu Jinmian Hou Yaxian Xin Ming Yuan Guoxuan Zhang Zhiyuan Yang | 2022 | Biomimetic Intelligence & Robotics2022，2，1： | 2 |
| 8 | Gaseous emissions from compressed natural gas buses in urban road and highway tests in China显示文摘The natural gas vehicle market is rapidly developing throughout the world, and the majority of such vehicles operate on compressed natural gas(CNG). However, most studies on the emission characteristics of CNG vehicles rely on laboratory chassis dynamometer measurements, which do not accurately represent actual road driving conditions. To further investigate the emission characteristics of CNG vehicles, two CNG city buses and two CNG coaches were tested on public urban roads and highway sections. Our results show that when speeds of 0–10 km/hr were increased to 10–20 km/hr, the CO_2, CO, nitrogen oxide(NO_x), and total hydrocarbon(THC) emission factors decreased by(71.6 ± 4.3)%,(65.6 ± 9.5)%,(64.9 ± 9.2)% and(67.8 ± 0.3)%, respectively. In this study, The Beijing city buses with stricter emission standards(Euro Ⅳ) did not have lower emission factors than the Chongqing coaches with Euro Ⅱ emission standards. Both the higher emission factors at 0–10 km/hr speeds and the higher percentage of driving in the low-speed regime during the entire road cycle may have contributed to the higher CO_2 and CO emission factors of these city buses. Additionally, compared with the emission factors produced in the urban road tests, the CO emission factors of the CNG buses in highway tests decreased the most(by 83.2%), followed by the THC emission factors, which decreased by 67.1%. | Tingting Yue Fahe Chai Jingnan Hu Ming Jia Xiaofeng Bao Zhenhua Li Liqang He Lei Zu | 2016 | Journal of Environmental Sciences2016，28，10： | 2 |
| 9 | Chitosan, a new and environmental benign electrode binder for use with graphite anode in lithium-ion batteries显示文摘 | Lili Chai Qunting Qu Longfei Zhang Ming Shen Li Zhang Honghe Zheng | 2013 | Electrochimica Acta2013，，： | 1 |
| 10 | Elevating kinetics of passivated Fe anodes with NH_(4)Cl regulator:Toward low-cost,long-cyclic and green cathode-free Fe-ion aqueous batteries显示文摘The environment benignity and battery cost are major concerns for grid-scale energy storage applications.The emerging dendrite-free Fe-ion aqueous batteries are promising due to the rich natural abundance,low cost and non-toxicity for Fe resources.However,serious passivation reactions on Fe anodes and poor long-term cyclability for matched cathodes still stand in the way for their practical usage.To settle above constraints,we herein use NH_(4)Cl as the electrolyte regulator to elevate the reaction kinetics of passivated Fe anodes,and also propose a special cathode-free design to prolong the cells lifetime over 1,000 cycles.The added NH_(4)Cl can erode/break inert passivation layers and strengthen the ion conductivity of electrolytes,facilitating the reversible Fe plating/stripping and Fe^(2+)shuttling.The highly puffed nano carbon foams function as current collectors and actives anchoring hosts,enabling expedite Fe^(2+)adsorption/desorption,FeII/FeIII redox conversions and FeIII deposition.The configured rocking-chair Fe-ion cells have good environmental benignity and decent energy-storage behaviors,including high reactivity/reversibility,outstanding cyclic stability and far enhanced operation longevity.Such economical,long-cyclic and green cathode-free Fe-ion batteries may hold great potential in near-future energy-storage power stations. | Shibo Chai Jianhui Zhu Jian Jiang Chang Ming Li | 2022 | Nano Research2022，15，4： | 1 |
| 11 | Multivariate Rational Response Surface Approximation of Nodal Displacements of Truss Structures显示文摘Polynomial-basis response surface method has some shortcomings for truss structures in structural optimization,concluding the low fitting accuracy and the great computational effort. Based on the theory of approximation, a response surface method based on Multivariate Rational Function basis(MRRSM) is proposed. In order to further reduce the computational workload of MRRSM, focusing on the law between the cross-sectional area and the nodal displacements of truss structure, a conjecture that the determinant of the stiffness matrix and the corresponding elements of adjoint matrix involved in displacement determination are polynomials with the same order as their respective matrices, each term of which is the product of cross-sectional areas, is proposed. The conjecture is proved theoretically for statically determinate truss structure, and is shown corrected by a large number of statically indeterminate truss structures. The theoretical analysis and a large number of numerical examples show that MRRSM has a high fitting accuracy and less computational effort. Efficiency of the structural optimization of truss structures would be enhanced. | Shan Chai Xiang-Fei Ji Li-Jun Li Ming Guo | 2018 | Chinese Journal of Mechanical Engineering2018，31，1： | 1 |
| 12 | Feasibility, cost-effectiveness and patients' acceptance of point-of-care INR testing in a hospital-based anticoagulation clicic显示文摘 | Ming Chai Kong Teong Guan Lim Heng Joo Ng Yiong Huak Chan Lai Heng Lee | 2008 | Ann Hematol2008，，87： | 1 |
| 13 | Neurotoxicity of low-dose repeatedly intranasal instillation of nano- and submicron-sized ferric oxide particles in mice显示文摘 | Bing Wang Weiyue Feng Motao Zhu Yun Wang Meng Wang Yiqun Gu Hong Ouyang Huajian Wang Ming Li Yuliang Zhao Zhifang Chai Haifang Wang | 2009 | Journal of Nanoparticle Research2009，，1： | 1 |
| 14 | Comparative study between core–shell and interpenetrating network structure polyurethane/polyacrylate composite emulsions显示文摘 | Shu Ling Chai Martin Ming Jin Hui Min Tan | 2008 | European Polymer Journal2008，，10： | 1 |
| 15 | Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans显示文摘 | Fang Li Jihua Chen Zhiguo Chai Ling Zhang Yuhong Xiao Ming Fang Sai Ma | 2008 | Journal of Dentistry2008，，4： | 1 |
| 16 | Structural Basis of Glycan Recognition in Globally Predominant Human P[8]Rotavirus显示文摘Rotavirus(RV)causes acute gastroenteritis in infants and children worldwide.Recent studies showed that glycans such as histo-blood group antigens(HBGAs)function as cell attachment factors affecting RV host susceptibility and prevalence.P[8]is the predominant RV genotype in humans,but the structural basis of how P[8]RVs interact with glycan ligands remains elusive.In this study,we characterized the interactions between P[8]VP8~*s and glycans which showed that VP8~*,the RV glycan binding domain,recognized both mucin core 2 and H type 1 antigens according to the ELISA-based oligosaccharide binding assays.Importantly,we determined the structural basis of P[8]RV-glycans interaction from the crystal structures of a Rotateq P[8]VP8~*in complex with core 2 and H type 1 glycans at 1.82.3?,respectively,revealing a common binding pocket and similar binding mode.Structural and sequence analysis demonstrated that the glycan binding site is conserved among RVs in the P[Ⅱ]genogroup,while genotype-specific amino acid variations determined different glycan binding preference.Our data elucidated the detailed structural basis of the interactions between human P[8]RVs and different host glycan factors,shedding light on RV infection,epidemiology,and development of anti-viral agents. | Xiaoman Sun Lei Dang Dandi Li Jianxun Qi Mengxuan Wang Wengang Chai Qing Zhang Hong Wang Ruixia Bai Ming Tan Zhaojun Duan | 2020 | Virologica Sinica2020，35，2： | 1 |
| 17 | The Effects of Gene Polymorphisms in Interleukin-4 and Interleukin-6 on the Susceptibility of Rheumatoid Arthritis in a Chinese Population显示文摘 | Xiang Li Wei Chai Ming Ni Meng Xu Zijian Lian Lewis Shi Yang Bai Yan Wang Wei Chiao Chang | 2014 | BioMed Research International2014，，： | 1 |
| 18 | Cadmium-induced oxidative stress and response of the ascorbate–glutathione cycle in Bechmeria nivea (L.) Gaud显示文摘 | Yunguo Liu Xin Wang Guangming Zeng Dan Qu Jiajia Gu Ming Zhou Liyuan Chai | 2007 | Chemosphere2007，，1： | 1 |
| 19 | Antibacterial functionalization of an experimental self-etching primer by inorganic agents: Microbiological and biocompatibility evaluations显示文摘 | Ming Fang Feng Chai Ji-Hua Chen Christel Neut Min Jia Yi Liu San-Jun Zhao Hartmut F. Hildebrand | 2007 | Biomolecular Engineering2007，，5： | 1 |
| 20 | No Relationship between Maternal Iron Status and Postpartum Depression in Two Samples in China显示文摘 | Rinat Armony-Sivan Jie Shao Ming Li Gengli Zhao Zhengyan Zhao Guobing Xu Min Zhou Jianying Zhan Yang Bian Chai Ji Xing Li Yaping Jiang Zhixiang Zhang Blair J. Richards Twila Tardif Betsy Lozoff Laura Murray-Kolb | 2012 | Journal of Pregnancy2012，，： | 1 |
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