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| 1 | Clinical application of specific antibody against glypican-3 for hepatocellular carcinoma diagnosis显示文摘Glypican-3(GPC3) is a promising tumor marker for hepatocellular carcinoma(HCC) diagnosis with high sensitivity and specificity.The aim of this study was to establish an immunohistochemical detection method for GPC3 using the 7D11 monoclonal antibody(7D11 mAb) and evaluate its application for HCC diagnosis.The feasibility of the 7D11 mAb was evaluated by immunohistochemistry performed on adjacent normal liver and intrahepatic cholangiocarcinoma(ICC) samples,Furthermore,the serum GPC3 levels were evaluated in 40 HCC patients,7 ICC patients and 50 healthy donors.The results showed that GPC3 was expressed in 85% of HCC tissues(34/40),but was undetectable in ICC tissues and adjacent normal tissues.GPC3 was significantly increased in the serum of HCC patients(17/40,42.5%) but was undetectable in the serum of ICC patients(0/7,0%) and healthy donors(0/50,0%).This prospective study evaluated the clinical usefulness of 7D11 mAb for GPC3 detection in HCC patients.In conclusion,the use of 7D11 mAb might be good for GPC3 large-scale applications for clinical diagnosis of HCC. | YU JuanPing MA Qiang ZHANG Bin MA RuiJuan XU XiGuang LI MingSong XU WeiWen LI Ming | 2013 | Science China(Life Sciences)2013，56，3： | 18 |
| 2 | SERK Receptor-like Kinases Control Division Patterns of Vascular Precursors and Ground Tissue Stem Cells during Embryo Development in Arabidopsis显示文摘During embryo development, the vascular precursors and ground tissue stem cells divide to renew them-selves and produce the vascular tissue, endodermal cells, and cortical cells. However, the molecular mech-anisms regulating division of these stem cells have remained largely elusive. In this study, we show that loss of function of SOMATIC EMBRYOGENESIS RECEPTOR-UKE KINASE (SERK) genes results in aberrant em-bryo development. Fewer cortical, endodermal, and vascular cells are generated in the embryos of serk1 serk2bak1 triple mutants. WUSCHEL-RELATED HOMBOBOX5 (WOXS) is ectopically expressed in vascular cells of serkl serk2 bak1 embryos. The first transverse division of vascular precursors in mid-globular em-bryos and second asymmetric division of ground tissue stem cells in early-heart embryos are abnormally altered to a longitudinal division. The embryo defects can be partially rescued by constitutively activated mitogen-activated protein kinase (MAPK) kinase kinase YODA (YDA) and MAPK kinase MKK5. Taken together, our results reveal that SERK-mediated signals regulate division patterns of vascular precursors and ground tissue stem cells, likely via the YDA-MKK4/5 cascade, during embryo development. | Huiqiang Li Zeping Cai Xiaojuan Wang Meizhen Li Yanwei Cui Nan Cui Fei Yang Mingsong Zhu Junxiang Zhao Wenbin Du Kai He Jing Yi Frans E.Tax Suiwen Hou Jia Li Xiaoping Gou | 2019 | Molecular Plant2019，12，7： | 7 |
| 3 | Astronomical time scale of the Turonian constrained by multiple paleoclimate proxies显示文摘One of the clocks that record the Earth history is(quasi-) periodic astronomical cycles.These cycles influence the climate that can be ultimately stored in sedimentary rocks.By cracking these(quasi-) periodic sedimentation signals,high resolution astronomical time scale(ATS) can be obtained.Paleoclimate proxies are widely used to extract astronomical cycles.However different proxies may respond differently to astronomical signals and nonastronomical noises including tectonics,diagenesis,and measurement error among others.Astronomical time scale constructed based on a single proxy where its signal-to-noise ratio is low may have uncertainty that is difficult to evaluate but can be revealed by utilizing other proxies.Here,we test eight astronomical age models using two astrochro no logical methods from four paleoclimate proxies(i.e.,color reflection L~* and b~*,natural gamma radiation,and bulk density) from the Turonian to the Coniacian of the Cretaceous Period at the Demerara Rise in the equatorial Atlantic.The two astrochronological methods are time calibration using long eccentricity bandpass filtering(E1 bandpass) and tracking the long eccentricity from evolutive harmonic analysis(tracking EHA).The statistical mean and standard deviation of four age models from the four proxies are calculated to construct one integrated age model with age uncertainty in each method.Results demonstrate that extracting astronomical signals from multiple paleoclimate proxies is a valid method to estimate age model uncertainties.Anchored at the Cenomanian/Turonian boundary with an age of 93.9 ± 0.15 Ma from biostratigraphy,the ages for CC11/CC12(calcareous nannofossil zones),Turonian/Coniacian(CC12/CC13),CC13/CC14,and Coniacian/Santonian boundaries are 91.25±0.20 Ma,89.87±0.20 Ma,86.36±0.33 Ma,and 86.03±0.32 Ma in E1 bandpass method,compared with 91.17±0.36 Ma,89.74±0.38 Ma,86.13±1.31 Ma,and 85.80±1.33 Ma respectively in tracking EHA method.These results are consistent with previous studies within error and provide a reliable estimation of uncertainties of the ages. | Chao Ma Mingsong Li | 2020 | Geoscience Frontiers2020，11，4： | 5 |
| 4 | A novel requirement analysis approach for periodic control systems显示文摘 | Zheng WANG Geguang PU Jiangwen LI Yuxiang CHEN Yongxin ZHAO Mingsong CHEN Bin GU Mengfei YANG Jifeng HE | 2013 | Frontiers of Computer Science2013，7，2： | 3 |
| 5 | Climate change response to astronomical forcing during the Oligocene-Miocene transition in the equatorial Atlantic(ODP Site 926)显示文摘The Oligocene-Miocene transition period was characterized by a decrease in global CO2 levels, expansion of polar ice sheet, fall in global sea-level, etc. However, the reasons for, and mechanisms of, this global, extreme-cold climate change event(Mi-1) still remain controversial. Our samples from the core of the Ocean Drilling Program(ODP) Leg 154, Site 926, located in the equatorial Atlantic, mainly consist of light-gray, nannofossil chalk with foraminifers interbedded with greenish-gray, clayey, nannofossil chalk sediments. Color variation from light-gray layers(up to 80% carbonate content) to dark layers(60% carbonate content) was observed to occur cyclically at the meter scale. Therefore, we chose color reflectance lightness(L*) data as the paleoclimate proxy on which to perform cyclostratigraphic analysis because it could reflect carbonate content changes. Based on the recognition of the 405 kyr long eccentricity and 40 kyr obliquity cycles of the L* series, we tuned the series to establish an absolute astronomical time scale using the published age of the Oligocene-Miocene boundary(OMB) as the anchor for an absolute age control point. The power spectra of the tuned L* series showed that the long eccentricity signals became significantly weak, while the obliquity signals became strong, from the Late Oligocene to the Early Miocene. The 405 kyr long eccentricity minimum coincided with the 1.2 Myr obliquity node at the OMB, and similar convergences might be closely related to other extreme-cold events in Earth’s history. In addition, the sedimentation accumulation rate, oxygen isotopes of benthonic foraminifers, and rodents’ per-taxon turnover rate from Central Spain showed the same 2 Myr cyclicity, which indicates the significant influence of Earth-orbital forcing on the Earth system and ecological evolution on the million-year time scale. | ZOU ZhuoYan HUANG ChunJu LI MingSong ZHANG Yang | 2016 | Science China Earth Sciences2016，59，8： | 3 |
| 6 | The CRL3^(BTBD9) E3 ubiquitin ligase complex targets TNFAIP1 for degradation to suppress cancer cell migration显示文摘Tumor necrosis factor alpha-induced protein 1(TNFAIP1)modulates a plethora of important biological processes,including tumorigenesis and cancer cell migration.However,the regulatory mechanism of TNFAIP1 degradation remains largely elusive.In the present study,with a label-free quantitative proteomic approach,TNFAIP1 was identified as a novel ubiquitin target of the Cullin-RING E3 ubiquitin ligase(CRL)complex.More importantly,Cul3-ROC1(CRL3),a subfamily of CRLs,was identified to specifically interact with TNFAIP1 and promote its polyubiquitination and degradation.Mechanistically,BTBD9,a specific adaptor component of CRL3 complex,was further defined to bind and promote the ubiquitination and degradation of TNFAIP1 in cells.As such,downregulation of BTBD9 promoted lung cancer cell migration by upregulating the expression of TNFAIP1,whereas TNFAIP1 deletion abrogated this effect.Finally,bioinformatics and clinical sample analyses revealed that BTBD9 was downregulated while TNFAIP1 was overexpressed in human lung cancer,which was associated with poor overall survival of patients.Taken together,these findings reveal a previously unrecognized mechanism by which the CRL3^(BTBD9) ubiquitin ligase controls TNFAIP1 degradation to regulate cancer cell migration. | Lihui Li Wenjuan Zhang Yue Liu Xiaojun Liu Lili Cai Jihui Kang Yunjing Zhang Wenlian Chen Changsheng Dong Yanmei Zhang Mingsong Wang Wenyi Wei Lijun Jia | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 2 |
| 7 | Conserved and differentiated functions of CIK receptor kinases in modulating stem cell signaling in Arabidopsis显示文摘The shoot apical meristem(SAM)and root apical meristem(RAM)act as pools of stem cells that give rise to aboveground and underground tissues and organs in higher plants,respectively.The CLAVATA3(CLV3)-WUSCHEL(WUS)negative-feedback loop acts as a core pathway controlling SAM homeostasis,while CLV3/EMBRYO SURROUNDING REGION(ESR)40(CLE40)and WUSCHEL-RELATED HOMEOBOX5(WOX5),homologs of CLV3 and WUS,direct columella stem cell fate.Moreover,CLV3 INSENSITIVE KINASES(CIKs)have been shown to be essential for maintaining SAM homeostasis,whereas whether they regulate the distal root meristem remains to be elucidated.Here,we report that CIKs are indispensable for transducing the CLE40 signal to maintain homeostasis of the distal root meristem.We found that the cik mutant roots displayed disrupted quiescent center and delayed columella stem cell(CSC)differentiation.Biochemical assays demonstrated that CIKs interact with ARABIDOPSIS CRINKLY4(ACR4)in a ligand-independent manner and can be phosphorylated by ACR4 in vitro.In addition,the phosphorylation of CIKs can be rapidly induced by CLE40,which partially depends on ACR4.Although CIKs act as conserved and redundant regulators in the SAM and RAM,our results demonstrated that they exhibit differentiated functions in these meristems. | Yafen Zhu Chong Hu Yanwei Cui Li Zeng Sunjingnan Li Mingsong Zhu Fanhui Meng Shuting Huang Li Long Jing Yi Jia Li Xiaoping Gou | 2021 | Molecular Plant2021，14，7： | 2 |
| 8 | Recrystallization nucleation under close-setδphase in a nickel-based superalloy during annealing显示文摘Annealing is one of the most efficient heat treatment to refine the grain microstructure in metal alloys.Prior to the annealing,an aging treatment can be implemented to precipitate the second phases for more nucleation sites in deformed mixed grains during subsequent recrystallization.However,the underlying mechanism behind the second phases and recrystallization has not yet been fully understood.Taking a typical deformed nickel(Ni)-based(GH4169)superalloy as an exapmle,this research aims to clairfy the connections between the recrystallization and delta(δ)phase(Ni_(3)Nb) during annealing.All deformed samples were also treated by aging and then annealing.The results show that the microstructure and component ofδphase dominated the recrystallization nucleation in the Ni-based alloy.Moreover,there was the presence of theδphase interval limiting nucleation(PILN)andδphase protecting nucleation(PPN),when a high content of close-set phase was precipitated from the matrix.The PILN determined the primary condition for the recrystallization nucleation that happended at the interval ofδphase larger than the critical nucleation size.The peak nucleation occurred in the PPN,where the recrystallization nucleus were free of the penetration by the adjacent grains,before the nucleation incubation time was reached.Thus,a new feasible JNtheory was proposed to determine the maximum domain of recrystallization nucleation under close-set precipitated phases.All the possible nucleation sites were assumed under PPN in the JNtheory.As such,the original microstructure can be expected to design for the annealing via the aging process.A quantitative evaluation of recrystallization nucleation can also achieved in the Ni-based alloys during aging. | Guanqiang Wang Mingsong Chen Yongcheng Lin Hongbin Li Yuqiang Jiang Yanyong Ma Chengxu Peng Jinliang Cai Quan Chen | 2022 | Journal of Materials Science & Technology2022，，20： | 1 |
| 9 | A Soy Protein-Based Composite Film with a Hierarchical Structure Inspired by Nacre显示文摘Soy protein-based composite film is a potential replacement for petroleum-based film with multipurpose applica-tions and cleaner production.It is difficult to improve both the tensile strength and toughness of a protein-based film without sacrificing its elongation.In this study,inspired by the hierarchical structure of nacre,a facile yet delicate strategy was developed to fabricate a novel bio-based film with excellent toughness and high strength.Triglycidylamine(TGA)crosslinked soy protein(SPI)as hard phase and thermoplastic polyurethane elastomer(TPU)as soft phase comprise an alternative lay-up hierarchical structure.The interface of these two phases is enhanced using triglycidylamine(TGA)surface-modified TPU(MTPU).The tensile strength of SPI/MTPU/TGA films increases by 392%to 7.78 MPa and their toughness increases by 391%to 8.50 MJ/m^(3) compared to soy protein/glycerol film.The proposed hierarchical structure can also be extended to other high-performance materials and polymers. | Yecheng Xu Chaojie Xu Mingsong Chen Jianzhang Li Ling Zeng Jing Luo Qiang Gao Sheldon Q.Shi | 2022 | Journal of Renewable Materials2022，10，3： | 1 |
| 10 | Convergence analysis of online gradient method for BP neural networks显示文摘 | Wei Wu Jian Wang Mingsong Cheng Zhengxue Li | 2010 | Neural Networks2010，，1： | 1 |
| 11 | Characterization of chlorine dioxide as disinfectant for the removal of low concentration microcystins显示文摘Microcystins,代表一个种导致癌的有机化合物,充满超营养作用水。为象反应机制一样的水里的低集中的 Microcystin-LR, Microcystin-RR,和 Microcystin 年的移动的氯二氧化物(ClO2 ) 上的反应因素的效果被使用连接酶的 immunosorbent 调查试金(ELISA ) 工具包和煤气的层析团 spectrometry (GCMS ) 。结果证明 MC-LR, MC-RR,和 MC 年能被 ClO2 高效地分解。降级效率断然被显示出相关到 ClO2 和反应时间的集中;当反应温度和 pH 的效果是细微的时。运动常数和有 ClO2 的 MC-LR , MC-RR ,和MC年的反应的激活精力作为 459.89 被决定, 583.15 , 488.43 Laphy团 spectrometry ( GCMS )被开发同时在表面水里分析五味道和气味混合物,即, 2-methylisoborneol ( 2-MIB ), 2,4,6-trichloroanisole ( TCA ), 2-isopropyl-3-methoxy pyrazine ( IPMP ), 2-isobutyl-3-methoxy pyrazine ( IBMP ),和 trans-1,10-dimethyl-trans-9-decalol ( geosmin , GSM )。集体 spectrometry 在选择离子监视被操作(SIM ) 模式。三种 SPE 列和三 eluting 解决吗? | Mingsong WU Junli HUANG Yuling ZHANG Shijie YOU Shaofeng LI Zhilin RAN Yu TIAN | 2012 | Frontiers of Environmental Science & Engineering2012，6，1： | 1 |
| 12 | A novel annealing method to uniformly refine deformed mixed grain microstructure of a solution-treated Ni-based superalloy显示文摘Increasing static recrystallization(SRX)nucleation rate and decreasing grain growth rate are the main ways to obtain uniform and fine grain microstructure by annealing treatment.However,it is contradictory to raise the SRX nucleation rate and reduce grain growth rate during isothermal annealing treatment.This is because the increase of the SRX nucleation rate needs to raise annealing temperature,while the decline of grain growth rate needs to reduce the annealing temperature.To solve the contradiction,a novel method named cooling recrystallization annealing treatment(CRT)is designed and verified.For the CRT,the relatively high annealing temperature in the primary stage can promote SRX nucleation,and the gradually decreased annealing temperature can reduce the growth rate of SRX grains.Besides,an aged treatment is first carried out to precipitate high content of theδphase before the CRT.Enoughδphases not only provide large numbers of sites for SRX nucleation but also limit the grain growth due to the pinning effect,especially in the primary high-temperature stage.The results show that the novel method can refine deformed mixed grain microstructure well when the suitable cooling rate,start and final recrystallization annealing temperatures are employed.The deformed mixed grain can be uniformly refined to an average size of 8.26μm with the route of 900℃×12 h+1020℃→970℃×20 min. | WANG GuanQiang CHEN MingSong LIN YongCheng LI HongBin ZENG WeiDong MA YanYong CAI JinLiang PENG ChengXu ZOU FenYang | 2021 | Science China(Technological Sciences)2021，64，8： | 1 |
| 13 | The Dof transcription factor COG1 acts as a key regulator of plant biomass by promoting photosynthesis and starch accumulation显示文摘Photosynthetic efficiency is the primary determinant of crop yield,including vegetative biomass and grain yield.Manipulation of key transcription factors known to directly control photosynthetic machinery can be an effective strategy to improve photosynthetic traits.In this study,we identified an Arabidopsis gain-of-function mutant,cogwheel1-3D,that shows a significantly enlarged rosette and increased biomass compared with wild-type plants.Overexpression of COG1,a Dof transcription factor,recapitulated the phenotype of cogwheel1-3D,whereas knocking out COG1 and its six paralogs resulted in a reduced rosette size and decreased biomass.Transcriptomic and quantitative reverse transcription polymerase chain reaction analyses demonstrated that COG1 and its paralogs were required for light-induced expression of genes involved in photosynthesis.Further chromatin immunoprecipitation and electrophoretic mobility shift assays indicated that COG1 can directly bind to the promoter regions of multiple genes encoding light-harvesting antenna proteins.Physiological,biochemical,and microscopy analyses revealed that COG1 enhances photosynthetic capacity and starch accumulation in Arabidopsis rosette leaves.Furthermore,combined results of bioinformatic,genetic,and molecular experiments suggested that the functions of COG1 in increasing biomass are conserved in different plant species.These results collectively demonstrated that COG1 acts as a key regulator of plant biomass by promoting photosynthesis and starch accumulation.Manipulating COG1 to optimize photosynthetic capacity would create new strategies for future crop yield improvement. | Zhuoyun Wei Haoyong Zhang Meng Fang Shuyuan Lin Mingsong Zhu Yuxiu Li Limin Jiang Tianliang Cui Yanwei Cui Hong Kui Liang Peng Xiaoping Gou Jia Li | 2023 | Molecular Plant2023，16，11： | 0 |
| 14 | Rethinking Data Center Networks:Machine Learning Enables Network Intelligence显示文摘To support the needs of ever-growing cloudbased services,the number of servers and network devices in data centers is increasing exponentially,which in turn results in high complexities and difficulties in network optimization.Machine learning(ML)provides an effective way to deal with these challenges by enabling network intelligence.To this end,numerous creative ML-based approaches have been put forward in recent years.Nevertheless,the intelligent optimization of data center networks(DCN)still faces enormous challenges.To the best of our knowledge,there is a lack of systematic and original investigations with in-depth analysis on intelligent DCN.To this end,in this paper,we investigate the application of ML to DCN optimization and provide a general overview and in-depth analysis of the recent works,covering flow prediction,flow classification,and resource management.Moreover,we also give unique insights into the technology evolution of the fusion of DCN and ML,together with some challenges and future research opportunities. | Bo Li Ting Wang Peng Yang Mingsong Chen Mounir Hamdi | 2022 | Journal of Communications and Information Networks2022，7，2： | 0 |
| 15 | Changes in intracellular and extracellular proteins after ERGIC3 knockdown in lung cancer:Proteins interacting with ERGIC3,HORN,and FLNA显示文摘Objective:Endoplasmic reticulum-Golgi intermediate compartment 3(ERGIC3)promotes cell proliferation and metastasis in lung cancer,but its molecular mechanism is unclear.Methods:The GLC-82 cells were randomly divided into the ERGIC3i group and the negative control group.The cells were transfected with ERGIC3 siRNA or control siRNA in the groups,respectively.The ERGIC3-interacting proteins expressed in cells or extracellularly were isolated by the immunoprecipitation method and detected by isobaric tags for relative and absolute quantitation and liquid chromatography-tandem mass spectrometry.The differentially expressed proteins were determined by bioinformatic methods.Results:After ERGIC3 knockdown,88 extracellular differentially expressed proteins,41 upregulated and 47 down-regulated,were detected in the supernatant of cultured cells.Among 52 intracellular differentially expressed proteins,33 were up-regulated and 19 down-regulated.Cluster analysis showed that the extracellular differential proteins are mainly involved in Ca2+binding and transport and I-kappa B kinase/NF-kappa B signal transduction.The upregulated proteins are mainly involved in the biological process of H3-K27 and H3-K4 methylation in cells.Co-immunoprecipitation assay showed that proteins interacting with ERGIC3 were rich in cytoskeleton construction and RHO GTPases activated p21-activated kinases.The intersection of these two research methods shows that ERGIC3 interacts with HORN and filamin A(FLNA).Conclusion:Proteomic analyses reveal that ERGIC3 acts as a vesicle transmembrane protein on the distribution of various extracellular and intracellular proteins and regulates the extracellular and intracellular biological processes by specifically binding hornin(HORN)and FLNA proteins.These findings maybe provide new methods and ideas for ERGIC3 as a therapeutic target for lung cancer. | WEI ZHAI XIANG ZHENG MENGYUAN LIU QIURONG ZHAO YUSHU ZHANG YUQING LUO SHANSHAN FENG JINJING WANG XUEYING LI MINGSONG WU | 2023 | BIOCELL2023，47，8： | 0 |
| 16 | Cobalt valence modulating in CoO_(x) incorporated carbon nanofiber for enhanced glucose electrooxidation显示文摘Glucose fuel cells(GFCs)driven by abiotic catalysts are promising green power sources for portable or wearable devices.In this work,a CoO_(x)incorporated carbon nanofiber(CoO_(x)@CNF)catalyst with mixed valences cobalt oxides have been developed through partial oxidation of pyrolyzed electrospun Co^(2+)/poly acrylonitrile fibers.The cobalt valence modulating could be achieved via regulating the incorporation ratio of cobalt acetate in precursors or the oxidation temperature of the pyrolyzed fibers.Electrocatalytic analyses show that the presence of CoO in CoO_(x)@CNF will provide more active sites for glucose electrooxidation,and thus enhance the electrocatalytic performance significantly.As a result,the glucose fuel cell built with the CoO.@CNF anode containing both CoO and Co_(3)O_(4)delivered a maximum power density of 270μW cm^(-2),which is higher than that of other reported Co_(3)O_(4)based GFCs.This work provides a simple strategy to develop excellent transition metal catalysts for GFCs to expand their applications in portable and wearable energy devices. | Jiadong Hu Hao Lu Mingsong Li Gang Xiao Min Li Xuemei Xiang Zhisong Lu Yan Qiao | 2022 | Materials Reports(Energy)2022，2，2： | 0 |
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