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| 1 | QTG-Seq Accelerates QTL Fine Mapping through QTL Partitioning and Whole-Genome Sequencing of Bulked Segregant Samples显示文摘Deciphering the genetic mechanisms underlying agronomic traits is of great importance for crop improvement. Most of these traits are controlled by multiple quantitative trait loci (QTLs), and identifying the underlying genes by conventional QTL fine-mapping is time-consuming and labor-intensive. Here, we devised a new method, named quantitative trait gene sequencing (QTG-seq), to accelerate QTL fine-mapping. QTGseq combines QTL partitioning to convert a quantitative trait into a near-qualitative trait, sequencing of bulked segregant pools from a large segregating population, and the use of a robust new algorithm for identifying candidate genes. Using QTG-seq, we fine-mapped a plant-height QTL in maize (Zea mays L.), qPH7, to a 300-kb genomic interval and verified that a gene encoding an NF-YC transcription factor was the functional gene. Functional analysis suggested that qPH7-encoding protein might influence plant height by interacting with a CO-like protein and an AP2 domain-containing protein. Selection footprint ana卜 ysis indicated that qPH7 was subject to strong selection during maize improvement. In summary, QTG-seq provides an efficient method for QTL fine-mapping in the era of “big data'. | Hongwei Zhang Xi Wang Qingchun Pan Pei Li Yunjun Liu Xiaoduo Lu Wanshun Zhong Minqi Li Linqian Han Juan Li Pingxi Wang Dongdong Li Yan Liu Qing Li Fang Yang Yuan-Ming Zhang Guoying Wang Lin Li | 2019 | Molecular Plant2019，12，3： | 13 |
| 2 | A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection显示文摘Large bone defects face a high risk of pathogen exposure due to open wounds,which leads to high infection rates and delayed bone union.To promote successful repair of infectious bone defects,fabrication of a scaffold with dual functions of osteo-induction and bacterial inhibition is required.This study describes creation of an engineered progenitor cell line(C3H10T1/2)capable of doxycycline(DOX)-mediated release of bone morphogenetic protein-2(BMP2).Three-dimensional bioprinting technology enabled creation of scaffolds,comprising polycaprolactone/mesoporous bioactive glass/DOX and bioink,containing these engineered cells.In vivo and in vitro experiments confirmed that the scaffold could actively secrete BMP2 to significantly promote osteoblast differentiation and induce ectopic bone formation.Additionally,the scaffold exhibited broad-spectrum antibacterial capacity,thereby ensuring the survival of embedded engineered cells when facing high risk of infection.These findings demonstrated the efficacy of this bioprinted scaffold to release BMP2 in a controlled manner and prevent the occurrence of infection;thus,showing its potential for repairing infectious bone defects. | Minqi Wang Hanjun Li Yiqi Yang Kai Yuan Feng Zhou Haibei Liu Qinghui Zhou Shengbing Yang Tingting Tang | 2021 | Bioactive Materials2021，6，5： | 3 |
| 3 | Polyurethane grouting materials with different compositions for the treatment of mud pumping in ballastless track subgrade beds:properties and application effect显示文摘Mud pumping in subgrade beds under ballastless tracks will deteriorate the dynamic performance of infrastructure under railway lines,reduce the smoothness of the railway lines,and seriously affect the comfort and safety of the trains.Due to their good mechanical properties,twocomponent polyurethane materials can be used for grouting to treat the fouling problems caused by ballastless track mud pumping.To develop a polyurethane formula suitable for the treatment of ballastless track mud pumping,we first performed indoor experiments to investigate the mechanical properties and gelation time of polyurethane elastomers synthesized with different raw material composition ratios,to determine an optimal composition ratio of the raw materials.Then,we conducted a dynamic field test to verify the remediation effect of the polyurethane material fabricated according to the design ratio.The results showed that polyurethane grouting material with the selected design ratios improved the contact characteristics between the surface layer of the subgrade bed and the base plate in the area,coordinating the dynamic response between the track structure and the subgrade bed.Thus,the obtained polyurethane grouting material could be used to renovate mud pumping areas of ballastless tracks with a good treatment effect. | Zhichao Huang Qian Su Junjie Huang Minqi Dong Dong Li Ting Liu | 2022 | Railway Engineering Science2022，30，2： | 1 |
| 4 | Carbon nanofibers membrane bridged with graphene nanosheet and hyperbranched polymer for high-performance osmotic energy harvesting显示文摘Reverse electrodialysis(RED),based on ion-selective membranes,is one of the most promising technologies for capturing osmotic energy.As key elements of the RED system,ion-selective membranes must meet the crucial demands of mechanical stability,anti-fouling characteristics,easy fabrication,and high power density;however,this still remains a challenge.In this study,we demonstrated a large-scale,mechanically stable,and high-porosity membrane obtained by combining carbon nanomaterials and hyperbranched polyethyleneimine(h-PEI),thereby achieving a high power density of 5.0 W·m−2 with seawater and river water.Carbon nanofibers(CNFs)were subsequently bridged with graphene and h-PEI to strengthen the interaction between the CNFs,reduce the channel size and increase the space charge density,mechanical strength,and toughness.The large-scale and mechanically stable membrane fabricated using the modified CNFs exhibited anion selectivity and high ionic conductivity,thereby achieving a high-performance osmotic energy conversion.Furthermore,the anti-fouling property of the membrane was confirmed by the stability of the osmotic energy conversion in a solution with algae,which can be attributed to the high porosity of carbon nanomaterials.This economic and convenient method for the ion-selective membrane preparation is believed to be promising for large-scale osmotic energy harvesting. | Weijie Wang Jinlin Hao Qian Sun Minqi Zhao Huiyi Liu Chao Li Xin Sui | 2023 | Nano Research2023，16，1： | 1 |
| 5 | A new benzaldehyde from the coral-derived fungus Aspergillus terreus C23-3 and its anti-inflammatory effects via suppression of MAPK signaling pathway in RAW264.7 cells显示文摘Marine fungi are important members of the marine microbiome,which have been paid growing attention by scientists in recent years.The secondary metabolites of marine fungi have been reported to contain rich and diverse compounds with novel structures(Chen et al.,2019).Aspergillus terreus,the higher level marine fungus of the Aspergillus genus(family of Trichocomaceae,order of Eurotiales,class of Euro・tiomycetes,phylum of Ascomycota). | Minqi CHEN Jinyue LIANG Yuan WANG Yayue LIU Chunxia ZHOU Pengzhi HONG Yi ZHANG Zhong-Ji QIAN | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，3： | 1 |
| 6 | Mitochondrial DNA genetic polymorphism in thirteen rice cytoplasmic male sterile lines显示文摘 | Ji Luan Tianran Liu Weiqi Luo Wen Liu Minqi Peng Wenjia Li Xiaojun Dai Manzhong Liang Liangbi Chen | 2013 | Plant Cell Reports2013，，4： | 1 |
| 7 | The relationships between step count and all-cause mortality and cardiovascular events:A doseresponse meta-analysis显示文摘Background:A goal of 10,000 steps per day is widely advocated,but there is little evidence to support that goal.Our purpose was to examine the doseresponse relationships between step count and all-cause mortality and cardiovascular disease risk.Methods:Cochrane Central Register of Controlled Trials,EMBASE,OVID,PubMed,Scopus,and Web of Science databases were systematically searched for studies published before July 9,2021,that evaluated the association between daily steps and at least 1 outcome.Results:Sixteen publications(12 related to all-cause mortality,5 related to cardiovascular disease;and 1 article contained 2 outcomes:both allcause death and cardiovascular events)were eligible for inclusion in the meta-analysis.There was evidence of a nonlinear doseresponse relationship between step count and risk of all-cause mortality or cardiovascular disease(p=0.002 and p=0.014 for nonlinearity,respectively).When we restricted the analyses to accelerometer-based studies,the third quartile had a 40.36%lower risk of all-cause mortality and a 35.05%lower risk of cardiovascular event than the first quartile(all-cause mortality:Q1=4183 steps/day,Q3=8959 steps/day;cardiovascular event:Q1=3500 steps/day,Q3=9500 steps/day;respectively).Conclusion:Our meta-analysis suggests inverse associations between higher step count and risk of premature death and cardiovascular events in middle-aged and older adults,with nonlinear doseresponse patterns. | Mingxin Sheng Junyue Yang Min Bao Tianzhi Chen Ruixue Cai Na Zhang Hongling Chen Minqi Liu Xueyu Wu Bowen Zhang Yiting Liu Jianqian Chao | 2021 | Journal of Sport and Health Science2021，10，6： | 0 |
| 8 | Host-Defense-Peptide-Mimickingβ-Peptide Polymer Acting as a Dual-Modal Antibacterial Agent by Interfering Quorum Sensing and Killing Individual Bacteria Simultaneously显示文摘Host defense peptides(HDPs)are one of the potentially promising agents for infection diseases due to their broad spectrum and low resistance rate,but their clinical applications are limited by proteolytic instability,high-cost,and complicated synthesis process.Here,we report a host-defense-peptidemimickingβ-peptide polymer that resists proteolysis to have enhanced the activity under physiological conditions,excellent antimicrobial efficiency even at high density of bacteria,and low cost for preparation.Theβ-peptide polymer demonstrated quorum sensing(QS)interference and bactericidal effect against both bacterial communities and individual bacterium to simultaneously block bacterial communication and disrupt bacterial membranes.The hierarchical QS network was suppressed,and main QS signaling systems showed considerably down-regulated gene expression,resulting in excellent biofilm eradication and virulence reduction effects.The dual-modal antibacterial ability possessed excellent therapeutic effects in Pseudomonas aeruginosa pneumonia,which could inhibit biofilm formation and exhibit better antibacterial and anti-inflammatory efficiency than clinically used antibiotics,levofloxacin.Furthermore,theβ-peptide polymer also showed excellent therapeutic effect Escherichia coli pyogenic liver abscess.Together,we believed that theβ-peptide polymer had a feasible clinical potential to treat bacterial infection diseases. | Wanlin Li Ximian Xiao Yuchen Qi Xiuhui Lin Huiqun Hu Minqi Shi Min Zhou Weinan Jiang Longqiang Liu Kang Chen Kai Wang Runhui Liu Min Zhou | 2023 | Research2023，，4： | 0 |
| 9 | Striatum shape development of the human fetal brain in early second trimester显示文摘The striatum is the largest structure of the basal ganglia,receiving synaptic input from multiple regions including the neocortex,thalamus,external globus pallidus,and midbrain.One of its major roles is to integrate sensorimotor,associative and limbic information.The development and evolution of basal ganglia is one of the most fundamental and important questions in neuroscience.Here,we segmented the fetal striatum using 7.0T MR images from 46 specimens from 14 to 22 gestational weeks and revealed the developmental course of the fetal striatum using volume and shape analyses.The absolute volume of caudate nucleus(CN)was linearly expanded and moderate hemisphere effect was observed in the absolute volume of CN.While the relative volume had a nonlinear relationship with GW,and there was no obvious difference between hemispheres.Both absolute and relative volumes of the putamen showed linear expansion,and there was no obvious difference between hemispheres. | Tian Mimi Xu Feifei Zhang Zhonghe Hu Minqi Liu Shuwei | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 10 | MRI-based morphological analysis of the adult brainstem during aging显示文摘The brainstem is a critical structure that possesses numerous neural nuclei,regulates vital autonomic functions,and closely related to many neurological disorders.The morphological changes of brainstem can reflect the condition of nuclei and might be early biomarkers of neurological disorders.We used strict brainstem segmentation criteria to manually segment 1.5T MRI of 187 normal adults aged from 19 to 70.There are obvious atrophy of volume of midbrain and medulla oblongata during aging process,and the pons was hardly changed. | Hu Minqi Liu Shuwei Tang Yuchun | 2021 | 解剖学杂志2021，44，S01： | 0 |
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