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| 1 | Mitochondrial cytochrome b sequence variations and phylogeny of the East Asian bagrid catfishes显示文摘The mitochondrial DNA cytochrome b gene was sequenced from 8 bagrid catfishes in China. Aligned with cytochrome b sequences from 9 bagrid catfishes in Japan, Korea and Russia retrieved from GenBank, and selected Silurus meridionalis, Liobagrus anguillicauda, Liobagrus reini and Phenacogrammus interruptus as outgroups, we constructed a matrix of 21 DNA sequences. The Kimura's two-parameter distances were calculated and molecular phylogenetic trees were constructed by using the maximum parsimony (MP) and neighbor-joining (NJ) methods. The results show that (i) there exist 3-bp deletions of mitochondrial cytochrome b gene compared with cypriniforms and characiforms; (ii) the molecular phylogenetic tree suggests that bagrid catfishes form a monophyletic group, and the genus Mystus is the earliest divergent in the East Asian bagrid catfishes, as well as the genus Pseudobagrus is a monophyletic group but the genus Pelteobagrus and Leiocassis are complicated; and (iii) the evolution rate of the East Asian bagrids mitochondrial cytochrome b gene is about 0.18%~0.30% sequence divergence per million years. | PENG Zuogang, HE Shunping and ZHANG Yaoguang(1. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 2. School of Life Science, Southwest China Normal University, Chongqing 400715, China) | 2002 | Progress in Natural Science:Materials International2002，12，6： | 5 |
| 2 | Origin of Gymnocypris przewalskii and phylogenetic history of Gymnocypris eckloni (Teleostei: Cyprinidae)显示文摘起源和种系发生的模式为 G 被估计。przexvalskii 和 G。由分析完全的 mtDNA 细胞色素 b 基因顺序(1140bp ) 的 eckloni。种系发生的分析进一步支持了那有三个 mtDNA 系(交流) ,在 G 识别。przewalskii 和 G。eckloni,证明第一鳃的外部 rakers 在 Gymnocypris 的发展史有很少意义钓鱼。建立的网络显示出那 G。黄河特定的 haplotype 艾尔的 eckloni 是一个创始人,它散发了 G 的所有 haplotypes。建议了 G 的 przexvalskii。przexvalskii 可能仅仅从 G 的二 maternals 之一发源。从黄河的 eckloni。F 测试和失配分析在 G 的人口显示出至少二个扩大事件。przexvalskii 大约 0.2734 妈并且 0。0658 妈,当时 G。ecklonifrom Qaidam 盆能经历了严重瓶颈效果大约 0.0693 妈。人口扩大与最近的普通祖先(TMRCA ) 在 subclades 艾尔和 A21 被检测大约 0.2308 ± 0 .01 妈和 0.1319 ± 0 .015 妈,分别地它在从上面的黄河引起了金海湖的分离的 Gonghe 运动事件的地质的年龄范围以内。这些结果建议了鱼多样化的效果由快速在迟了的更新世 Qing-hai-Tibetan 高原高举。 | Zhao Kai Duan Ziyuan Yang Gongshe Peng Zuogang He Shunping Chen Yiyu | 2007 | Progress in Natural Science:Materials International2007，17，5： | 3 |
| 3 | Methodology for fish biodiversity monitoring with environmental DNA metabarcoding:The primers,databases and bioinformatic pipelines显示文摘Environmental DNA(eDNA)originates from cellular material shed by organisms into aquatic or terrestrial environments and can be sampled and monitored using metabarcoding technology,which is revolutionizing fish biodiversity monitoring.Several reviews concerning fish eDNA have focused on standard sampling methods and its applications,though a systematic review focused on marker genes,databases,and bioinformatic pipelines has not yet been published.Here,we present a comprehensive literature review of studies applying metabarcoding technology to fish eDNA for the purpose of fish biodiversity monitoring.We systematically provide the available universal primers used to amplify barcoding sequences from fish eDNA,and then discuss reference barcoding databases,relevant bioinformatic analyses,as well as developed pipelines.The performances of universal primers and their relevant reference databases are summarized.Combined use of multiple primer pairs targeted for more than one gene marker(e.g.,12S,16S,Cytb,COI),and use of both local and public databases are recommended as approaches to improve the sensitivity and reliability of fish eDNA analyses.We also compare the effectiveness of eDNA metabarcoding to traditional approaches for monitoring fish biodiversity and highlight challenges and future perspectives associated with this new tool.Ultimately,we advocate for greater incorporation of eDNA analysis into fish biodiversity assessments to assist environmental managers. | Fan Xiong Lu Shu Honghui Zeng Xiaoni Gan Shunping He Zuogang Peng | 2022 | Water Biology and Security2022，1，1： | 3 |
| 4 | Phylogeogra- phy of the endemic Gymnocypris chilianensis ( Cyprinidae ) : se- quential westward colonization followed by allopatric evolution in re-sponse to cyclical Pleistocene glaciations on the Tibetan Plateau 显示文摘 | ZHAO Kai DUAN Ziyuan PENG Zuogang | 2011 | Mol Phylogenet Evol2011，59，2： | 1 |
| 5 | Sympatric or micro-allopatric speciation in a glacial lake?Genomic islands support neither显示文摘Apparent cases of sympatric speciation may actually be due to micro-allopatric or micro-parapatric speciation.One way to distinguish between these models is to examine the existence and nature of genomic islands of divergence,wherein divergent DNA segments are interspersed with low-divergence segments.Such islands should be rare or absent under micro-allopatric speciation but common in cases of speciation with gene flow.Sympatric divergence of endemic fishes is known from isolated saline,crater,postglacial,and ancient lakes.Two morphologically distinct cyprinid fishes,Gymnocypris eckloni scoliostomus(GS)and G.eckloni eckloni(GE),in a small glacial lake on the Qinghai-Tibet Plateau,Lake Sunmcuo,match the biogeographic criteria of sympatric speciation.In this study,we examined genome-wide variation in 46individuals from these two groups.The divergence time between the GS and GE lineages was estimated to be 20-60 Kya.We identified 54 large genomic islands(≥100 kb)of speciation,which accounted for 89.4%of the total length of all genomic islands.These islands harboured divergent genes related to olfactory receptors and olfaction signals that may play important roles in food selection and assortative mating in fishes.Although the genomic islands clearly indicated speciation with gene flow and rejected micro-allopatric speciation,they were too large to support the hypothesis of sympatric speciation.Theoretical and recent empirical studies suggested that continual gene flow in sympatry should give rise to many small genomic islands(as small as a few kilobases in size).Thus,the observed pattern is consistent with the extensive evidence on parapatric speciation,in which adjacent habitats facilitate divergent selection but also permit gene flow during speciation.We suggest that many,if not most,of the reported cases of sympatric speciation are likely to be micro-parapatric speciation. | Ning Sun Liandong Yang Fei Tian Honghui Zeng Ziwen He Kai Zhao Cheng Wang Minghui Meng Chenguang Feng Chengchi Fang Wenqi Lv Jing Bo Yongtao Tang Xiaoni Gan Zuogang Peng Yiyu Chen Shunping He | 2022 | National Science Review2022，9，12： | 0 |
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