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| 1 | Regulation of FN1 degradation by the p62/SQSTM1-dependent autophagy-lysosome pathway in HNSCC显示文摘Epithelial–mesenchymal transition(EMT)is involved in both physiological and pathological processes.EMT plays an essential role in the invasion,migration and metastasis of tumours.Autophagy has been shown to regulate EMT in a variety of cancers but not in head and neck squamous cell carcinoma(HNSCC).Herein,we investigated whether autophagy also regulates EMT in HNSCC.Analyses of clinical data from three public databases revealed that higher expression of fibronectin-1(FN1)correlated with poorer prognosis and higher tumour pathological grade in HNSCC.Data from SCC-25 cells demonstrated that rapamycin and Earle’s balanced salt solution(EBSS)promoted autophagy,leading to increased FN1 degradation,while 3-methyladenine(3-MA),bafilomycin A1(Baf A1)and chloroquine(CQ)inhibited autophagy,leading to decreased FN1 degradation.On the other hand,autophagic flux was blocked in BECN1 mutant HNSCC Cal-27 cells,and rapamycin did not promote autophagy in Cal-27 cells;also in addition,FN1 degradation was inhibited.Further,we identified FN1 degradation through the lysosome-dependent degradation pathway using the proteasome inhibitor MG132.Data from immunoprecipitation assays also showed that p62/SQSTM1 participated as an autophagy adapter in the autophagy–lysosome pathway of FN1 degradation.Finally,data from immunoprecipitation assays demonstrated that the interaction between p62 and FN1 was abolished in p62 mutant MCF-7 and A2780 cell lines.These results indicate that autophagy significantly promotes the degradation of FN1.Collectively,our findings clearly suggest that FN1,as a marker of EMT,has adverse effects on HNSCC and elucidate the autophagy–lysosome degradation mechanism of FN1. | Xinchen Liu Lin Meng Xing Li Daowei Li Qilin Liu Yumeng Chen Xiangwei Li Wenhuan Bu Hongchen Sun | 2020 | International Journal of Oral Science2020，12，4： | 7 |
| 2 | Novel neutrophil extracellular trap-related mechanisms in diabetic wounds inspire a promising treatment strategy with hypoxia-challenged small extracellular vesicles显示文摘Neutrophil extracellular traps(NETs)have been considered a significant unfavorable factor for wound healing in diabetes,but the mechanisms remain unclear.The therapeutic application of small extracellular vesicles(sEVs)derived from mesenchymal stem cells(MSCs)has received considerable attention for their properties.Hypoxic preconditioning is reported to enhance the therapeutic potential of MSC-derived sEVs in regenerative medicine.Therefore,the aim of this study is to illustrate the detailed mechanism of NETs in impairment of diabetic wound healing and develop a promising NET-targeting treatment based on hypoxic pretreated MSC-derived sEVs(Hypo-sEVs).Excessive NETs were found in diabetic wounds and in high glucose(HG)-induced neutrophils.Further research showed that high concentration of NETs impaired the function of fibroblasts through activating endoplasmic reticulum(ER)stress.Hypo-sEVs efficiently promoted diabetic wound healing and reduced the excessive NET formation by transferring miR-17-5p.Bioinformatic analysis and RNA interference experiment revealed that miR-17-5p in Hypo-sEVs obstructed the NET formation by targeting TLR4/ROS/MAPK pathway.Additionally,miR-17-5p overexpression decreased NET formation and overcame NET-induced impairment in fibroblasts,similar to the effects of Hypo-sEVs.Overall,we identify a previously unrecognized NET-related mechanism in diabetic wounds and provide a promising NET-targeting strategy for wound treatment. | Ziqiang Chu Qilin Huang Kui Ma Xi Liu Wenhua Zhang Shengnan Cui Qian Wei Huanhuan Gao Wenzhi Hu Zihao Wang Sheng Meng Lige Tian Haihong Li Xiaobing Fu Cuiping Zhang | 2023 | Bioactive Materials2023，，9： | 1 |
| 3 | Applications of 2D-Layered Palladium Diselenide and Its van der Waals Heterostructures in Electronics and Optoelectronics显示文摘The rapid development of two-dimensional(2D)transition-metal dichalcogenides has been possible owing to their special structures and remarkable properties.In particular,palladium diselenide(PdSe_(2))with a novel pentagonal structure and unique physical characteristics have recently attracted extensive research inter-est.Consequently,tremendous research progress has been achieved regarding the physics,chemistry,and electronics of PdSe_(2).Accordingly,in this review,we recapitulate and summarize the most recent research on PdSe_(2),including its structure,properties,synthesis,and appli-cations.First,a mechanical exfoliation method to obtain PdSe_(2) nanosheets is introduced,and large-area synthesis strate-gies are explained with respect to chemical vapor deposition and metal selenization.Next,the electronic and optoelectronic properties of PdSe_(2) and related hetero-structures,such as field-effect transistors,photodetectors,sensors,and thermoelec-tric devices,are discussed.Subsequently,the integration of systems into infrared image sensors on the basis of PdSe_(2) van der Waals heterostructures is explored.Finally,future opportunities are highlighted to serve as a general guide for physicists,chemists,materials scientists,and engineers.Therefore,this com-prehensive review may shed light on the research conducted by the 2D material community. | Yanhao Wang Jinbo Pang Qilin Cheng Lin Han Yufen Li Xue Meng Bergoi Ibarlucea Hongbin Zhao Feng Yang Haiyun Liu Hong Liu Weijia Zhou Xiao Wang Mark HRummeli Yu Zhang Gianaurelio Cuniberti | 2021 | Nano-Micro Letters2021，13，9： | 1 |
| 4 | Turning gray selenium into a nanoaccelerator of tissue regeneration by PEG modification显示文摘Selenium(Se)is an essential trace element involved in nearly all human physiological processes but suffers from a narrow margin between benefit and toxicity.The nanoform of selenium has been proven shown to be more bioavailable and less toxic,yet significant challenges remain regarding the efficient and feasible synthesis of biologically active nanoselenium.In addition,although nanoselenium has shown a variety of biological activities,more interesting nanoselenium features are expected.In this work,hydrosoluble nanoselenium termed Nano-Se in the zero oxidation state was synthesized between gray Se and PEG.A zebrafish screen was carried out in zebrafish larvae cocultured with Nano-Se.Excitingly,Nano-Se promoted the action of the FGFR,Wnt,and VEGF signaling pathways,which play crucial roles in tissue regeneration.As expected,Nano-Se not only achieved the regeneration of zebrafish tail fins and mouse skin but also promoted the repair of skin in diabetic mice while maintaining a profitable safe profile.In brief,the Nano-Se reported here provided an efficient and feasible method for bioactive nanoselenium synthesis and not only expanded the application of nanoselenium to regenerative medicine but also likely reinvigorated efforts for discovering more peculiarunique biofunctions of nanoselenium in a great variety of human diseases. | Jieqiong Cao Yibo Zhang Peiguang Zhang Zilei Zhang Bihui Zhang Yanxian Feng Zhixin Li Yiqi Yang Qilin Meng Liu He Yulin Cai Zhenyu Wang Jie Li Xue Chen Hongwei Liu An Hong Wenjie Zheng Xiaojia Chen | 2022 | Bioactive Materials2022，7，9： | 0 |
| 5 | Enhanced bioavailability and biosafety of cannabidiol nanomicelles for effective anti-inflammatory therapy显示文摘Cannabidiol(CBD)shows great anti-inflammatory potential;however,the hydrophobicity and strong first-pass effect of CBD leads to its extremely low oral bioavailability.Poloxamer 407(P407)is a tri-block copolymer composed of(poly)ethylene oxide(PEO)and(poly)propylene oxide(PPO)sections.It has a PEO-PPO-PEO structure,which is widely used in the preparation of drug delivery systems that are highly biocompatible.When it reaches a certain concentration in water,P407 can self-assemble into a micelle structure containing a hydrophobic core and a hydrophilic shell.A potential approach to enhanc-ing the oral bioavailability of hydrophobic drugs incorporating them into the hydrophilic carrier.We prepared CBD nanomicelles with a drug loading of 14.29%by a cosolvent evaporation method using P407 with appropriate antioxidants.Cell experiments indicated that anti-inflammatory markers(IL-4 and IL-10)increased,while inflammatory markers(TNF-αand IL-6)decreased.Moreover,animal experiments showed that inflammatory cells were inhibited by CBD nanomicelles,and the anti-inflammatory effect of micelles was better than that of CBD,while no obvious evidence indicated toxicity to the liver and kidney. | Yuan Rao Runwei Li Saixing Liu Linchao Meng Qilin Wu Qipeng Yuan Hao Liang Meng Qin | 2022 | Particuology2022，20，10： | 0 |
| 6 | 毛黏液星形细胞瘤的临床病理观察(英文)显示文摘Objective:The aim of this study was to study the clinicopathological and immunohistochemical features of pilomyxoid astrocytoma(PMA).Methods:The clinical and pathologic features in six cases of PMA were analyzed.Immunohistochemical staining for glial fibrillary acidic protein(GFAP),synaptophysin(Syn),Chromogranin A(CgA),cytokeratin(AE1/AE3),epithelial membrane antigen(EMA)and Ki67 was performed on paraffin-embedded sections.Results:Among the six cases,five occurred in female patients,one was male,the age at diagnosis ranged from 2 to 15 years.Four cases were located in the hypothalamic area and optic pathway,one case in the third ventricle,and one case in left parietal lobe.On imaging,PMAs often appears as well-circumscribed mass.Microscopically,the tumor was composed of monomorphous bipolar(piloid)cells setting in a prominent myxoid background with an angiocentric radiating growth pattern in some areas.PMA lacked biphasic pattern,Rosenthal fibers and eosinophilic granular bodies which were usually typical in a classic pilocytic astrocytoma(PA).Immunohistochemcal study showed that the tumor cells were diffusely positive for GFAP.Syn positive staining was observed in one case.The Ki67 labeling index measured less than 5%.Conclusion:PMA is a distinct aggressive variant of pilocytic astrocytoma with special histological and immunohistochemical features.It is typically a rare tumor of early childhood.Immunohistochemical staining for GFAP and Syn is helpful in differential diagnosis. | Zixuan Yang Fei Yan Li Meng Qilin Ao Pengcheng Zhu | 2013 | The Chinese-German Journal of Clinical Oncology2013，12，9： | 0 |
| 7 | Monolithically integrated low-voltage soft e-skins designed to emulate biological sensorimotor loop显示文摘Skin plays a pivotal role in the human body’s interaction with the external environment,enabling detection and response to sensory stimuli^([1,2]).However,skin damage can impede the sensorimotor system,with severe cases often requiring amputation and resulting in the inability to perform basic movements^([3]).While prosthetic limbs can aid in certain movement processes to a limited extent,they also possess constraints in terms of perception and flexibility. | Qilin Hua Haixing Meng Guozhen Shen | 2023 | Science China Materials2023，66，11： | 0 |
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