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| 1 | Whole-genome sequencing provides insights into the genetic diversity and domestication of bitter gourd(Momordica spp.)显示文摘Bitter gourd(Momordica charantia)is a popular cultivated vegetable in Asian and African countries.To reveal the characteristics of the genomic structure,evolutionary trajectory,and genetic basis underlying the domestication of bitter gourd,we performed whole-genome sequencing of the cultivar Dali-11 and the wild small-fruited line TR and resequencing of 187 bitter gourd germplasms from 16 countries.The major gene clusters(Bi clusters)for the biosynthesis of cucurbitane triterpenoids,which confer a bitter taste,are highly conserved in cucumber,melon,and watermelon.Comparative analysis among cucurbit genomes revealed that the Bi cluster involved in cucurbitane triterpenoid biosynthesis is absent in bitter gourd.Phylogenetic analysis revealed that the TR group,including 21 bitter gourd germplasms,may belong to a new species or subspecies independent from M.charantia.Furthermore,we found that the remaining 166 M.charantia germplasms are geographically differentiated,and we identified 710,412,and 290 candidate domestication genes in the South Asia,Southeast Asia,and China populations,respectively.This study provides new insights into bitter gourd genetic diversity and domestication and will facilitate the future genomics-enabled improvement of bitter gourd. | Junjie Cui Yan Yang Shaobo Luo Le Wang Rukui Huang Qingfang Wen Xiaoxia Han Nansheng Miao Jiaowen Cheng Ziji Liu Changyuan Zhang Chengcheng Feng Haisheng Zhu Jianwen Su Xinjian Wan Fang Hu Yu Niu Xiaoming Zheng Yulan Yang Dai Shan Zhensheng Dong Weiming He Narinder P.S.Dhillon Kailin Hu | 2020 | Horticulture Research2020，7，1： | 7 |
| 2 | Reduction of Patulin in Apple Cider by UV Radiation显示文摘 | Dong Qingfang Manns David C Feng Guoping Yue Tianli Churey John J Worobo Randy W | 2010 | Journal of Food Protection2010，，1： | 1 |
| 3 | Amultimedia fate model to evaluate the fate of PAHs inSonghua River,China 显示文摘 | WANG Ce FENG Yujie SUN Qingfang | 2012 | Environmental Pollution2012，164，： | 1 |
| 4 | Polycyclic aromatic hydrocarbons in surface water fromthe Songhua River of China 显示文摘 | FENG Yujie SUN Qingfang GAO Peng | 2009 | FreseniusEnvironmental Bulletin2009，18，12： | 1 |
| 5 | A multimedia fate model to evaluate the fate of PAHs in Songhua River,China显示文摘 | Wang Ce Feng Yujie Sun Qingfang | 2012 | Environ Pollut2012，164，： | 1 |
| 6 | Polycyclic aromatic hydrocarbons in surface water from the Songhua River of China 显示文摘 | FENG Yujie SUN Qingfang GAO Peng | 2009 | Fresenius Environmental Bulletin2009，18，12： | 1 |
| 7 | Reduction of Patulin in Apple Cider by UV Radiation显示文摘 | Dong Qingfang Manns David C Feng Guoping Yue Tianli Churey John J Worobo Randy W | 2010 | Journal of Food Protection2010，，1： | 1 |
| 8 | A multimedia fate model to evaluate the fate of PAHs in Songhua River, China显示文摘 | WANG Ce FENG Yujie SUN Qingfang | 2012 | Environment Pollution2012，164，： | 1 |
| 9 | Ammonia Volatilization from Soils Fertilized with Different Nitrogen Type and Application Method in Germination and Early Seedling Stages from the Radish Field显示文摘Ammonia volatilization(AV) from basal fertilizer with different nitrogen(N) types and application methods was investigated by the ventilation method in germination and early seedling stages during radish growth seasons in 2014. Four N fertilizer types, urea(U), ammonium bicarbonate(AB), ammonia sulfate(AS), and controlled urea formaldehyde(CUF) were applied through 5 cm depth placement(I) and 10 cm depth placement(II). The results showed that the N fertilizer type was the main factor that caused AV loss in germination and early seedling stages from the radish field. The highest and the lowest cumulative AV losses in germination and early seedling stages from the radish fields were 33.23 and 11.21 N kg/hm^2 for the treatments of AB+I and CUF+II, respectively, accounting for 60.40 and 26.40% of the N application for each treatment. The 10 cm deep placement of N reduced AV rates and lagged the AV process, and CUF significantly reduced ammonia volatilization. The data showed that the suitable N fertilizer type and application method for basal fertilizer were CUF and deep placement, respectively.Therefore, fertilizing with proper N fertilizer types and methods should be the efficient measures to mitigate AV losses from the radish field and will alleviate environment problems. | Weiling YUAN Shangyong YUAN Feng ZHANG Xiaohui DENG Caixia GAN Lei CUI Qingfang WANG | 2016 | Agricultural Science & Technology2016，17，4： | 1 |
| 10 | A multimedia fate model to evaluate the fate of PAHs in Songhua River, China显示文摘 | Ce Wang Yujie Feng Qingfang Sun Shanshan Zhao Peng Gao Bai-Lian Li | 2012 | Environmental Pollution2012，，： | 1 |
| 11 | Variation of Electromagnetic Tornogram Before and After Earthquakes显示文摘Cross-well electromagnetic tomography has been used to monitor the changes of the abso rption coefficient of electromagnetic waves before and after earthquakes for 5 years at Dahuichang fault since 1992.It has been found that 3 months before the occurrence of moderately strong earthquakes(ML≥4.0,△<210 km),several special changes in tomogram appeared as follows:The mean-value of the absorption coefficient dropped about 10% compared with the annually decreasing tendency; the mean difference of pixels suddenly decreased or increased in different directions; and the entropy of the paragenic matrix in a tomogram decreased and the number of low absorption pixels increased.It is inferred that such changes in electromagnetic tomograms are attributed to the variation of micro-cracks and porosity in rocks.Electromagnetic tomography can be studied as a new technique to detect earthquake precursors. | Sun Cichang,Wang Xusheng,Liu Qingfang,Tao Yulu,Yu Ping,and Feng RuiInstitute of Geophysics,SSB,Beijing 100081,China Department of Geophysics,Peking University,Beijing 100871,China Seismnlogical Bureau of Hunan Province,Changsha 410001,China Center for Seismological Data and Information,SSB,Beijing 100045,China | 1998 | Earthquake Research in China1998，12，2： | 0 |
| 12 | Identification and analysis of a minority fraction in a U87 cell line Do side-population cells represent bona fide stem cell-like cancer cells?显示文摘BACKGROUND:Overwhelming evidence suggests that tumor bulks are comprised of differentiated tumor cells and cancer stem cells(CSCs).The stem cell-like side-population(SP) cells account for a minor fraction of the total tumor cells,yet are apparently the cells capable of tumor initiation,growth, maintenance,and recurrence. OBJECTIVE:To identify potential stem cell-like cancer cells in a U87 human brain glioma cell line on the basis of dye efflux,clone formation,and multi-drug resistance capacity. DESIGN,TIME AND SETTING:The cellular and molecular biology experiment was performed at the Laboratory of Shanghai Institute of Hematology and Laboratory of Shanghai Institute of Endocrinology in Ruijin Hospital;in vivo contrast observational animal trial was performed at Experimental Animal Center,School of Medicine,Shanghai Jiao Tong University from June 2007 to May 2008. MATERIALS:The U87 cell line was provided by the Shanghai Institute of Cancer Research, Chinese Academy of Science;DMEM/F12(1:1) and fetal bovine serum were purchased from Gibco, Invitrogen,USA;human recombinant basic fibroblast growth factors were purchased from BD Bioscience,USA;Hoechst 33342,Verapamil,and methyl thiazolyl tetrazolium were purchased from Sigma,USA;phycoerythrin-labeled anti-human-CD133 was purchased from Milteny Biotec, Germany;SYBR(?) PrimeScript^(TM) RT-PCR kit was purchased from TaKaRa Biotechnology,Dalian, China. METHODS:Monolayer cultured cells were harvested by 0.25%Trypsin-EDTA and suspended at a 1×10~6/mL dilution in PBS containing 2%FBS,and were stained with Hoechst 33342 dye,either alone or in combination with Verapamil.Following fluorescence-activated cell sorting,SP and non-SP subsets were cultivated with serum-containing(DMEM plus 10%fetal bovine serum) or serum-free culture medium[DMEM/F12(1:1) + 1×B27 supplement + 10 ng/mL basic fibroblast growth factors + 1×L-glutamine]to determine growth characteristics in vitro.Finally,single free U87 cells and subsets(SP or non-SP cells) were subcutaneously injected into the backs of 5-week-old nude mice for in vivo tumorigenicity. MAIN OUTCOME MEASURES:Cell morphology and clonogenicity were observed under inverted microscope;SP phenotype and fluorescent antibody labeling were analyzed by MoFlo^(TM) flow cytometry;ABC transporter mRNA expression was evaluated by semi-quantitative real-time RT-PCR;efflux capacity for anti-neoplastic drugs from the U87 cell line and subsets was measured with the MTT assay,then detected by enzyme-linked immunosorbent assay at a wavelength of 490 nm;in vivo tumorigenicity in immunodeficient nude mice was evaluated by diameter size. RESULTS:During in vitro passages,human U87 cells maintained a stable SP fraction profile and exhibited the ability to form neurosphere-like clones.SP cell proliferation decreased compared with non-treated U87 cells.CD133 expression was reduced in the SP and non-SP cells.Freshly sorted SP fractions expressed higher levels of ABC drug transporter genes,and exhibited increased potential for cytotoxic drug resistance.The in vivo malignancy of U87 cells was largely dependent on non-SP cells in nude mice,and tumors that formed from the non-SP fraction developed faster and larger compared with tumors from the SP fraction. CONCLUSION:The SP cell component was a key factor that influenced mRNA expression and cytotoxic drug resistance.In particular,cancer stem cells or tumor-initiating cells were not exclusively enriched in the SP subset of the U87 cell line,and non-SP cells were even more tumorigenic. | Changwei Gu Qingfang Sun Jiang Zhu Liuguan Bian Weiguo Zhao Jiankang Shen HengGao Dongxia Feng | 2009 | Neural Regeneration Research2009，4，11： | 0 |
| 13 | Construction of Nanocarriers Based on Endogenous Cell Membrane and Their Application in Nanomedicine显示文摘Inspired by the adaptation of cells to the surrounding environment,the combination of cell membrane and nanomaterials has gradually become a new type of biomimetic nanocomposite.The construction of endogenous membrane biomimetic nanocarriers(EMBNs)not only retains the structure of membrane surface protein,but also has the properties of nanoparticles(NPs),also provides the ability of natural interaction between nanomaterials and organisms,thus overcoming the severe challenges faced by traditional nanodelivery systems in clinical application.In this paper,the construction methods of EMBNs are reviewed,focusing on a simple method to prepare membrane proteins.Secondly,EMBNs were classified according a variety of cell membranes.Finally,the unique advantages of EMBNs in nanomedicine are introduced,including improving biocompatibility,homologous targeting ability,prolonging blood circulation time and immune escape abilty.In nanomedicine,such as targeted delivery,phototherapy,immunotherapy and tumor imaging,multifaceted biological interfaces through membrane masking provide a new approach for the development of multifunctional NPs. | Yingshu Guo Wenxin Li Shiwei Liu Dan Jing Yifan Wang Qingfang Feng Kaixiang Zhang Jing-Juan Xu | 2022 | Chinese Journal of Chemistry2022，40，13： | 0 |
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