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| 1 | Soil Bacterial Communities Under Different Long-Term Fertilization Regimes in Three Locations Across the Black Soil Region of Northeast China显示文摘Although soil bacteria play critical roles in agro-ecosystems, the knowledge of their response to long-term fertilization across the black soil region of Northeast China is limited. In this study, we sequenced 16 S rRNA genes to assess the effects of four long-term fertilization regimes—non-fertilization(NoF), chemical fertilizer(CF), manure(M), and chemical fertilizer plus manure(CFM)—on soil properties and bacterial communities in three locations, the northern, middle, and southern parts, across the black soil region. Results showed that the influence of fertilization regimes on soil properties varied significantly among the three locations. Manure fertilization significantly increased microbial biomass carbon and relative abundance of copiotrophic bacteria. Principal component analysis(PCA)showed that the total bacterial communities were separated into three groups according to the sampling location despite long-term fertilization, and that soil pH was the most important factor in shifting bacterial communities. In addition, similar fertilization regimes resulted in different influences on bacterial community composition, and the most influential soil properties varied among the three locations. Our results highlighted that geographical separation was a more dominant factor affecting bacterial communities than fertilization, and that long-term similar fertilization regimes did not induce consistent changes in bacterial community composition in the black soil region. | HU Xiaojing LIU Junjie WEI Dan ZHU Ping CUI Xi'an ZHOU Baoku CHEN Xueli JIN Jian LIU Xiaobing WANG Guanghua | 2018 | Pedosphere2018，28，5： | 17 |
| 2 | Directly patterned substrate-free plasmonic “nanograter” structures with unusual Fano resonances显示文摘The application of three-dimensional(3D)plasmonic nanostructures as metamaterials(MMs),nano-antennas,and other devices faces challenges in producing metallic nanostructures with easily definable orientations,sophisticated shapes,and smooth surfaces that are operational in the optical regime and beyond.Here,we demonstrate that complex 3D nanostructures can be readily achieved with focused-ion-beam irradiation-induced folding and examine the optical characteristics of plasmonic“nanograter”structures that are composed of free-standing Au films.These 3D nanostructures exhibit interesting 3D hybridization in current flows and exhibit unusual and well-scalable Fano resonances at wavelengths ranging from 1.6 to 6.4 mm.Upon the introduction of liquids of various refractive indices to the structures,a strong dependence of the Fano resonance is observed,with spectral sensitivities of 1400 nm and 2040 nm per refractive index unit under figures of merit of 35.0 and 12.5,respectively,for low-order and high-order resonance in the near-infrared region.This work indicates the exciting,increasing relevance of similarly constructed 3D free-standing nanostructures in the research and development of photonics and MMs. | Ajuan Cui Zhe Liu Jiafang Li Tiehan H Shen Xiaoxiang Xia Zhiyuan Li Zhijie Gong Hongqiang Li Benli Wang Junjie Li Haifang Yang Wuxia Li Changzhi Gu | 2015 | Light(Science & Applications)2015，4，1： | 8 |
| 3 | Whole-genome sequencing provides insights into the genetic diversity and domestication of bitter gourd(Momordica spp.)显示文摘Bitter gourd(Momordica charantia)is a popular cultivated vegetable in Asian and African countries.To reveal the characteristics of the genomic structure,evolutionary trajectory,and genetic basis underlying the domestication of bitter gourd,we performed whole-genome sequencing of the cultivar Dali-11 and the wild small-fruited line TR and resequencing of 187 bitter gourd germplasms from 16 countries.The major gene clusters(Bi clusters)for the biosynthesis of cucurbitane triterpenoids,which confer a bitter taste,are highly conserved in cucumber,melon,and watermelon.Comparative analysis among cucurbit genomes revealed that the Bi cluster involved in cucurbitane triterpenoid biosynthesis is absent in bitter gourd.Phylogenetic analysis revealed that the TR group,including 21 bitter gourd germplasms,may belong to a new species or subspecies independent from M.charantia.Furthermore,we found that the remaining 166 M.charantia germplasms are geographically differentiated,and we identified 710,412,and 290 candidate domestication genes in the South Asia,Southeast Asia,and China populations,respectively.This study provides new insights into bitter gourd genetic diversity and domestication and will facilitate the future genomics-enabled improvement of bitter gourd. | Junjie Cui Yan Yang Shaobo Luo Le Wang Rukui Huang Qingfang Wen Xiaoxia Han Nansheng Miao Jiaowen Cheng Ziji Liu Changyuan Zhang Chengcheng Feng Haisheng Zhu Jianwen Su Xinjian Wan Fang Hu Yu Niu Xiaoming Zheng Yulan Yang Dai Shan Zhensheng Dong Weiming He Narinder P.S.Dhillon Kailin Hu | 2020 | Horticulture Research2020，7，1： | 7 |
| 4 | Endothelial PDGF-BB/PDGFR-βsignaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation显示文摘The mechanisms that coordinate the shift from joint homeostasis to osteoarthritis(OA)remain unknown.No pharmacological intervention can currently prevent the progression of osteoarthritis.Accumulating evidence has shown that subchondral bone deterioration is a primary trigger for overlying cartilage degeneration.We previously found that H-type vessels modulate aberrant subchondral bone formation during the pathogenesis of OA.However,the mechanism responsible for the elevation of H-type vessels in OA is still unclear.Here,we found that PDGFR-βexpression,predominantly in the CD31^(hi)Emcn^(hi) endothelium,was substantially elevated in subchondral bones from OA patients and rodent OA models.A mouse model of OA with deletion of PDGFR-βin endothelial cells(ECs)exhibited fewer H-type vessels,ameliorated subchondral bone deterioration and alleviated overlying cartilage degeneration.Endothelial PDGFR-βpromotes angiogenesis through the formation of the PDGFR-β/talin1/FAK complex.Notably,endothelium-specific inhibition of PDGFR-βby local injection of AAV9 in subchondral bone effectively attenuated the pathogenesis of OA compared with that of the vehicle-treated controls.Based on the results from this study,targeting PDGFR-βis a novel and promising approach for the prevention or early treatment of OA. | Zhuang Cui Hangtian Wu Ye Xiao Ting Xu Junjie Jia Hancheng Lin Rongmin Lin Kun Chen Yihuang Lin Kaiqun Li Xiaohu Wu Changjun Li Bin Yu | 2022 | Bone Research2022，10，4： | 4 |
| 5 | Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: a comparison study显示文摘Seabuckthorn seed meal(SSM) is a waste of oil extraction industry that rich in protein. In order to seek suitable protein extraction method, three different deep eutectic solvents(DESs)(including choline chlorideglycerol, choline chloride-oxalic acid and choline chloride-urea) were developed for extracting protein from SSM and compared with alkaline. Result indicated that alkaline could effectively extract 56.9% protein from SSM and its protein content was 73.1%, higher than DES at 31.0%-41.4% and 64.3%-67.5%, respectively. However, compared to alkali, DES led to a product with less β-sheet, more β-turn, more essential amino acids, higher total amino acid content, especially choline chloride-urea which extracted protein showing an integrated and similar protein weight distribution compared to SSM. Also, this protein extracted chloride-urea showed a highest digestibility in vitro(by pepsin)(54.2%). These results indicated that choline chloride-urea extraction is better than alkaline extraction for SSM. | Junjie Lin Huan Xiang Dongxiao Sun-Waterhouse Chun Cui Wei Wang | 2022 | Food Science and Human Wellness2022，11，4： | 4 |
| 6 | First stirling-type cryocooler reaching lambda point of ~4He(2.17K) and its prospect in Chinese HUBS satellite project显示文摘The issue of 'missing baryons” is always an unsolved mystery in galaxy cosmology, and an important reason for the great uncertainty of the galaxies' formation and evolution. To find the 'missing' baryons, extra high-resolution spectral observation and imaging of the soft X-ray band (<1 kev) is required. However, the existing observation methods cannot meet this requirement. Therefore, Tsinghua University leads and proposes the Chinese 'Hot Universe Baryon Surveyor (HUBS)' satellite program. | Jue Wang Changzhao Pan Tong Zhang Kaiqi Luo Xiaotong Xi Xianlin Wu Jianpeng Zheng Liubiao Chen Junjie Wang Yuan Zhou Hai Jin Wei Cui | 2019 | Science Bulletin2019，64，4： | 3 |
| 7 | Double lock of a potent human therapeutic monoclonal antibody against SARS-CoV-2显示文摘Receptor recognition and subsequent membrane fusion are essential for the establishment of successful infection by SARS-CoV-2.Halting these steps can cure COVID-19.Here we have identified and characterized a potent human monoclonal antibody,HB27,that blocks SARS-CoV-2 attachment to its cellular receptor at sub-nM concentrations.Remarkably,HB27 can also prevent SARS-CoV-2 membrane fusion.Consequently,a single dose of HB27 conferred effective protection against SARS-CoV-2 in two established mouse models.Rhesus macaques showed no obvious adverse events when administrated with10 times the effective dose of HB27.Cryo-EM studies on complex of SARS-CoV-2 trimeric S with HB27 Fab reveal that three Fab fragments work synergistically to occlude SARS-CoV-2 from binding to the ACE2 receptor.Binding of the antibody also restrains any further conformational changes of the receptor binding domain,possibly interfering with progression from the prefusion to the postfusion stage.These results suggest that HB27 is a promising candidate for immuno-therapies against COVID-19. | Ling Zhu Yong-Qiang Deng Rong-Rong Zhang Zhen Cui Chun-Yun Sun Chang-Fa Fan Xiaorui Xing Weijin Huang Qi Chen Na-Na Zhang Qing Ye Tian-Shu Cao Nan Wang Lei Wang Lei Cao Huiyu Wang Desheng Kong Juan Ma Chunxia Luo Yanjing Zhang Jianhui Nie Yao Sun Zhe Lv Neil Shaw Qianqian Li Xiao-Feng Li Junjie Hu Liangzhi Xie Zihe Rao Youchun Wang Xiangxi Wang Cheng-Feng Qin | 2021 | National Science Review2021，8，3： | 3 |
| 8 | Chronic effects of different fertilization regimes on nirS-type denitrifier communities across the black soil region of Northeast China显示文摘Denitrification is one of the major processes causing nitrogen loss from arable soils.This study aimed to investigate the responses of nir S-type denitrifier communities to different chronic fertilization regimes across the black soil region of Northeast China.Soil samples were collected from sites located in the north(NB),middle(MB),and south(SB)of the black soil region of Northeast China,each with four chronic fertilization regimes:no fertilizer(No F),chemical fertilizer(CF),manure(M),and chemical fertilizer plus manure(CFM).Methods of quantitative polymerase chain reaction(q PCR)and Illumina Mi Seq sequencing were applied to assess the abundance and composition of denitrifier communities by targeting the nir S gene.The results showed that the M and CFM regimes significantly increased the abundances of nir S-type denitrifiers compared with No F at the three locations.The majority of nir S sequences were grouped as unclassified denitrifiers,and the different fertilizers induced little variation in the relative abundance of known nir S-type denitrifier taxa.Over 90%of the sequences were shared among the four fertilization regimes at each location,but none of the abundant operational taxonomic units(OTUs)were shared among the three locations.Principal coordinate analysis(PCo A)revealed that the communities of nir S-type denitrifier were separated into three groups that corresponded with their locations.Although similar fertilization regimes did not induce consistent changes in the nir S-type denitrifier communities,soil p H and NO-3-N content simultaneously and significantly influenced the structure of nir S-type denitrifier communities at the three locations.Our results highlight that geographical separation rather than chronic fertilization was the dominant factor determining the nir S-type denitrifier community structures,and similar chronic fertilization regimes did not induce consistent shifts of nir S-type denitrifier communities in the black soils. | Xiaojing HU Junjie LIU Dan WEI Ping ZHU Xi'an CUI Baoku ZHOU Xueli CHEN Jian JIN Xiaobing LIU Guanghua WANG | 2020 | Pedosphere2020，30，1： | 3 |
| 9 | Profiles and Source Apportionment of Nonmethane Volatile Organic Compounds in Winter and Summer in Xi’an, China, based on the Hybrid Environmental Receptor Model显示文摘Summer and winter campaigns for the chemical compositions and sources of nonmethane hydrocarbons(NMHCs)and oxygenated volatile organic compounds(OVOCs)were conducted in Xi’an.Data from 57 photochemical assessment monitoring stations for NMHCs and 20 OVOC species were analyzed.Significant seasonal differences were noted for total VOC(TVOC,NMHCs and OVOCs)concentrations and compositions.The campaign-average TVOC concentrations in winter(85.3±60.6 ppbv)were almost twice those in summer(47.2±31.6 ppbv).Alkanes and OVOCs were the most abundant category in winter and summer,respectively.NMHCs,but not OVOCs,had significantly higher levels on weekends than on weekdays.Total ozone formation potential was higher in summer than in winter(by 50%)because of the high concentrations of alkenes(particularly isoprene),high temperature,and high solar radiation levels in summer.The Hybrid Environmental Receptor Model(HERM)was used to conduct source apportionment for atmospheric TVOCs in winter and summer,with excellent accuracy.HERM demonstrated its suitability in a situation where only partial source profile data were available.The HERM results indicated significantly different seasonal source contributions to TVOCs in Xi’an.In particular,coal and biomass burning had contributions greater than half in winter(53.4%),whereas traffic sources were prevalent in summer(53.1%).This study’s results highlight the need for targeted and adjustable VOC control measures that account for seasonal differences in Xi’an;such measures should target not only the severe problem with VOC pollution but also the problem of consequent secondary pollution(e.g.,from ozone and secondary organic aerosols). | Jian SUN Zhenxing SHEN Yue ZHANG Wenting DAI Kun HE Hongmei XU Zhou ZHANG Long CUI Xuxiang LI Yu HUANG Junji CAO | 2021 | Advances in Atmospheric Sciences2021，38，1： | 2 |
| 10 | Homeobox B8 Targets Sterile Alpha Motif Domain-Containing Protein 9 and Drives Glioma Progression显示文摘Gliomas are the most commonly occurring tumors of the central nervous system. Glioblastoma multiforme (GBM) is the most malignant and aggressive brain cancer in adults. Further understanding of the mechanisms underlying the aggressive nature of GBM is urgently needed. Here we identified homeobox B8(HOXB8), a member of the homeobox family, as a crucial contributor to the aggressiveness of GBM. Data mining of publicly accessible RNA sequence datasets and our patient cohorts confirmed a higher expression of HOXB8 in the tumor tissue of GBM patients, and a strong positive correlation between the expression level and pathological grading of tumors and a negative correlation between the expression level and the overall survival rate. We next showed that HOXB8 promotes the proliferation and migration of glioblastoma cells and is crucial for the activation of the PI3K/AKT pathway and expression of epithelial–mesenchymal transition-related genes, possibly through direct binding to the promoter of SAMD9 (Sterile Alpha Motif Domain-Containing Protein 9) and activating its transcription. Collectively, we identified HOXB8 as a critical contributor to the aggressiveness of GBM, which provides insights into a potential therapeutic target for GBM and opens new avenues for improving its treatment outcome. | Wenping Ma Hongze Jin Wenjie Liu Xiaojuan Li Xingang Zhou Xinwu Guo Runfa Tian Qi Cui Junjie Luo Yueying Jiao Youtao Yu Haifeng Yang Hongshan Zhao | 2020 | Neuroscience Bulletin2020，36，4： | 2 |
| 11 | The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication显示文摘Rice stripe virus(RSV)transmitted by the small brown planthopper causes severe rice yield losses in Asian countries.Although viral nuclear entry promotes viral replication in host cells,whether this phenomenon occurs in vector cells remains unknown.Therefore,in this study,we systematically evaluated the presence and roles of RSV in the nuclei of vector insect cells.We observed that the nucleocapsid protein(NP)and viral genomic RNAs were partially transported into vector cell nuclei by utilizing the importin a nuclear transport system.When blocking NP nuclear localization,cytoplasmic RSV accumulation significantly increased.In the vector cell nuclei,NP bound the transcription factor YY1 and affected its positive regulation to FAIM.Subsequently,decreased FAIM expression triggered an antiviral caspase-dependent apoptotic reaction.Our results reveal that viral nuclear entry induces completely different immune effects in vector and host cells,providing new insights into the balance between viral load and the immunity pressure in vector insects. | Wan Zhao Junjie Zhu Hong Lu Jiaming Zhu Fei Jiang Wei Wang Lan Luo Le Kang Feng Cui | 2022 | Protein & Cell2022，13，5： | 2 |
| 12 | Bursty event detection from collaborative tags显示文摘 | Junjie Yao Bin Cui Yuxin Huang | 2012 | World Wide Web2012，15，： | 1 |
| 13 | The enhanced upconversion fluorescence and almost unchanged particle size of β-NaYF 4 :Yb 3+ , Er 3+ nanoparticles by codoping with K + ions显示文摘 | Zhiqin Liang Yue Cui Suling Zhao Lijiao Tian Junjie Zhang Zheng Xu | 2014 | Journal of Alloys and Compounds2014，，： | 1 |
| 14 | Helical carbon nanotubes: Intrinsic peroxidase catalytic activity and its application for biocatalysis and biosensing显示文摘 | Cui Rongjing Han Zhida Zhu junjie | 2011 | Chemistry2011，17，34： | 1 |
| 15 | Real-time measurements of PM_(2.5),PM_(10-2.5),and BC in an urban street canyon显示文摘A continuous dichotomous beta gauge monitor was used to characterize the hourly content of PM_(2.5).PM_(10-2.5).and Black Carbon(BC)over a 12-month period in an urban street canyon of Hong Kong.Hourly vehicle counts for nine vehicle classes and meteorological data were also recorded.The average weekly cycles of PM_(2.5),PM_(10-2.5).and BC suggested that all species are related to traffic,with high concentrations on workdays and low concentrations over the weekends.PM_(2.5) exhibited two comparable concentrations at 10:00-11:00(63.4 μg/m^3) and 17:00-18:00(65.0 μg/m^3) local time(LT) during workdays,corresponding to the hours when the numbers of diesel-fueled and gasoline-fueled vehicles were at their maximum levels:3179 and 2907 h^(-1),respectively.BC is emitted mainly by diesel-fueled vehicles and this showed the highest concentration(31.2 μg/m^3) during the midday period(10:00-11:00 LT) on workdays.A poor correlation was found between PM_(2.5) concentration and wind speed(R = 0.51,P-value> 0.001).In contrast,the concentration of PM_(10-2.5) was found to depend upon wind speed and it increased with obvious statistical significance as wind speed increased(R = 0.98,P-value< 0.0001). | Yan Cheng Shun Cheng Lee Yuan Gao Long Cui Wenjing Deng Junji Cao Zhenxing Shen Jian Sun | 2015 | Particuology2015，13，3： | 1 |
| 16 | An InDel-based linkage map of hot pepper ( Capsicum annuum )显示文摘 | Weipeng Li Jiaowen Cheng Zhiming Wu Cheng Qin Shu Tan Xin Tang Junjie Cui Li Zhang Kailin Hu | 2015 | Molecular Breeding2015，，1： | 1 |
| 17 | Proteomic analysis of the mitochondria from Caenorhabditis Elegans显示文摘Mitochondria are one of the most important organelles in a cell, playing essential roles in cell physiological processes including energy production, metabolism, ion homeostasis, aging | Jing Li, Tanxi Cai, Peng Wu, Ziyou Cui, Xiulan Chen, Junjie Hou, Zhensheng Xie, Peng Xue, Pingsheng Liu, Fuquan Yang Proteomic Platform & National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, 100101 | 2009 | 生物物理学报2009，25，S1： | 1 |
| 18 | Bursty event detection from collaborative tags显示文摘 | Yao Junjie Cui Bin Huang Yuxin | 2012 | WorldWide Web2012，15，2： | 1 |
| 19 | One-pot synthesis of hyperbranched poly(amido amine) clicked with a sugar shell via Michael addition polymerization and thiol click reaction显示文摘This paper reports the production of glycopolymers via a simple and flexible method.A novel glycopolymer with a hyperbranched poly(amido amine) core and a sugar shell (HPAA-GLc) was synthesized by using thiol-ene click reaction via facile one-pot method.Hyperbranched poly(amido amine) with vinyl terminals was first synthesized by Michael addition polymerization of N,N'-methylene bisacrylamide (MBA) with 1-(2-aminoethyl) piperazine (AEPZ).Subsequently,thiol-ene click reaction between vinyl units of hyperbranched poly(amido amine) and thio-glucose was performed in situ.Based on the NMR result,all the vinyl groups reacted with thiol-glucose in 120 min.Strong photoluminescence emission was observed from the aqueous solution of HPAA-GLc. | YU ZhiQiang,CUI MengMeng,YAN JunJie & YOU YeZi CAS Key Lab of Soft Matter Chemistry Department of Polymer Science and Engineering,University of Science and Technology of China,Hefei 230026,China | 2010 | Science China Chemistry2010，53，8： | 1 |
| 20 | High impact of vehicle and solvent emission on the ambient volatile organic compounds in a major city of northwest China显示文摘Monitoring of ambient volatile organic compounds(VOCs)was conducted within typical residentialcommercial area in the city of Xi’an in northwest China during typical ozone(O_(3))episodes,to investigate the major contributors to the characteristic of ambient VOCs and their impact on O_(3) production.In the residential-commercial area,diurnal variation of VOCs was highly impacted by vehicle exhaust,fuel evaporation,and local solvent use.Relative higher contributions(up to 60%)of VOCs from solvent use to the ozone formation potential were found.The present findings highlight the urgent need for restrictions on the emission of VOCs from solvent use and non-vehicle-traffic-related sources,such as oil storage. | Yonggang Xue Liqin Wang Suixin Liu Yu Huang Long Chen Long Cui Yan Cheng Junji Cao | 2022 | Chinese Chemical Letters2022，33，5： | 1 |
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