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| 1 | Development of cell-based pseudovirus entry assay to identify potential viral entry inhibitors and neutralizing antibodies against SARS-CoV-2显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the causative virus of the coronavirus disease 2019(COVID-19)pandemic.To establish a safe and convenient assay system for studying entry inhibitors and neutralizing antibodies against SARS-CoV-2,we constructed a codon-optimized,full-length C-terminal mutant spike(S)gene of SARS-CoV-2.We generated a luciferase(Luc)-expressing pseudovirus containing the wild-type or mutant S protein of SARS-CoV-2 in the envelope-defective HIV-1 backbone.The key parameters for this pseudovirus-based assay,including the S mutants and virus incubation time,were optimized.This pseudovirus contains a Luc reporter gene that enabled us to easily quantify virus entry into angiotensin-converting enzyme 2(ACE2)-expressing 293T cells.Cathepsin(Cat)B/L inhibitor E64d could significantly block SARS-CoV-2 pseudovirus infection in 293T-ACE2 cells.Furthermore,the SARS-CoV-2 spike pseudotyped virus could be neutralized by sera from convalescent COVID-19 patients or recombinant ACE2 with the fused Fc region of human IgG1.Thus,we developed a pseudovirus-based assay for SARS-CoV-2,which will be valuable for evaluating viral entry inhibitors and neutralizing antibodies against this highly pathogenic virus. | Jie Hu Qingzhu Gao Changlong He Ailong Huang Ni Tang Kai Wang | 2020 | Genes & Diseases2020，7，4： | 15 |
| 2 | Grassland degradation in Northern Tibet based on remote sensing data显示文摘This study selected vegetation cover as the main evaluation index, calculated the grassland degradation index (GDI) and established the remote sensing monitoring and evaluation system for grassland degradation in Northern Tibet, according to the National Standard (GB19377-2003), based on the remote sensing data such as NDVI data derived from NOAA/AVHRR with a spatial resolution of 8 km of 1981-2000, from SPOT/VGT with a spatial resolution of 1 km of 2001 and from MODIS with a spatial resolution of 0.25 km of 2002-2004 respectively in this area, in combination with the actual condition of grassland degradation. The grassland degradation processes and their responses to climate change during 1981-2004 were discussed and analyzed in this paper. The result indicated that grassland degradation in Northern Tibet is very serious, and the mean value of GDI in recent 20 years is 2.54 which belongs to the serious degradation grade. From 1981 to 2004, the GDI fluctuated distinctly with great interannual variations in the proportion of degradation degree and GDI but the general tendency turned to severe-grade during this period with the grassland degradation grade changed from light degraded to serious degraded in Northern Tibet. The extremely serious degraded and serious degraded grassland occupied 1.7% and 8.0% of the study area, the moderate and light degraded grassland accounted for 13.2% and 27.9% respectively, and un-degraded grassland occupied 49.2% of the total grassland area in 2004. The grassland degradation was serious, especially in the conjunctive area of Naqu, Biru and Jiali counties, the headstream of the Yangtze River lying in the Galadandong snow mountain and glaciers, the area along the Qinghai-Tibet highway and railway, and areas around the Tanggula and Nianqingtanggula snow mountains and glaciers. So the snow mountains and glaciers as well as their adjacent areas in Northern Tibet were sensitive to climate change and the areas along the vital communication line with frequent human activities experienced relatively serious grassland degradation. | GAO Qingzhu LI Yu'e WAN Yunfan LIN Erda XIONG Wei JIANGCUN Wangzha WANG Baoshan LI Wenfu | 2006 | Journal of Geographical Sciences2006，16，2： | 10 |
| 3 | Size Distributions of Aerosol Sulfates and Nitrates in Beijing during the 2008 Olympic Games: Impacts of Pollution Control Measures and Regional Transport显示文摘为 2008 个奥运会,激烈的控制措施在二氧化物(SO2 ) ,氮氧化物(没有 x ) 和处理穷人的问题的另外的污染物质通风的硫的工业、城市的排出物上被实现在北京的质量。在微粒硫酸盐和硝酸盐集中以及他们的尺寸分布上调查 SO2 和没有 x 减小的效果,分离尺寸的喷雾器样品在照原尺寸的控制前后在北京在城市、顺风的农村地点用微孔的一致存款 impactors (MOUDI ) 被收集。在采样时期期间,在城市、农村的地点的好粒子(PM1.8 ) 的集体集中分别地是 94.0 和 85.9 g m3。超过 90% 硫酸盐和 60% 硝酸盐形成了同样好的粒子。得益于有益的气象学的条件和来源控制,硫酸盐在奥林匹克期间在相当低的集中并且首先在冷凝作用模式被观察。控制措施的效果独立被分析让北风和南方的 air-mass-dominated 天说明任何偏爱。在控制措施被实现以后,当在北方的空气群众占优势时,下午,硫酸盐,和硝酸盐显著地被减少,与在更大的粒子的减小的一个更高的百分比。在控制措施被实现以后,微滴模式粒子,在控制被实现以前,统治了硫酸盐和硝酸盐,显著地在集体集中被减少。但当弄脏的南方的空气群众占优势时,本地来源控制在北京测量,有效地没从北方中国平原由于庞大的地区性的贡献减少周围的硫酸盐集中。 | WANG Xinfeng WANG Tao Ravi Kant PATHAK Mattias HALLQUIST GAO Xiaomeit NIE Wei XUE Likun GAO Jian GAO Rui ZHANG Qingzhu WANG Wenxing WANG Shulan CHAI Fahe CHEN Yizhen | 2013 | Advances in Atmospheric Sciences2013，30，2： | 8 |
| 4 | Transcriptome-wide identification and expression profiling of Pinus massoniana MYB transcription factors responding to phosphorus deficiency显示文摘Myeloblastosis(MYB) proteins constitute one of the largest transcription factor(TF) families in plants and play crucial roles in regulating plant physiological and biochemical processes, including adaptation to diverse abiotic stresses. These TF families contain highly conserved MYB repeats(1 R, R2 R3, 3 R and 4 R) at the N-terminus. Roles for MYB TFs have been reported in response to such stresses as dehydration, salt, cold, and drought. The characterization of Masson pine(Pinus massoniana) MYB TFs are reported, including the analysis of MYB TFs expression in seedlings under controlled conditions and two different phosphate(Pi) deficient treatments. By searching for conserved MYB motifs in full transcriptomic RNA sequencing data for P. massoniana, 59 sequences were identified as MYB TFs. Conserved domainstructures and comparative functional and phylogenetic relationships of these MYB TFs with those in Arabidopsis were assessed using various bioinformatics tools. Based on microarray analysis, P. massoniana MYB genes exhibited different expression patterns under the two Pi deficiency conditions. Genes encoding MYB TFs that showed increased expression under critical Pi deficiency were identified, and some MYBs were differentially expressed only under conditions of severe Pi starvation. These results are useful for the functional characterization of MYB TFs that may be involved in the response to Pi deficiency and play divergent roles in plants. | Fuhua Fan Qingzhu Wang Xiaopeng Wen Guijie Ding | 2020 | Journal of Forestry Research2020，31，3： | 4 |
| 5 | A theoretical study of hydrogen-bonded molecular clusters of sulfuric acid and organic acids with amides显示文摘Amides,a series of significant atmospheric nitrogen-containing volatile organic compounds(VOCs),can participate in new particle formation(NPF)throught interacting with sulfuric acid(SA)and organic acids.In this study,we investigated the molecular interactions of formamide(FA),acetamide(AA),N-methylformamide(MF),propanamide(PA),Nmethylacetamide(MA),and N,N-dimethylformamide(DMF)with SA,acetic acid(HAC),propanoic acid(PAC),oxalic acid(OA),and malonic acid(MOA).Global minimum of clusters were obtained through the association of the artificial bee colony(ABC)algorithm and density functional theory(DFT)calculations.The conformational analysis,thermochemical analysis,frequency analysis,and topological analysis were conducted to determine the interactions of hydrogen-bonded molecular clusters.The heterodimers formed a hepta or octa membered ring through four different types of hydrogen bonds,and the strength of the bonds are ranked in the following order:SO-H···O>CO-H···O>N-H···O>C-H···O.We also evaluated the stability of the clusters and found that the stabilization effect of amides with SA is weaker than that of amines with SA but stronger than that of ammonia(NH 3)with SA in the dimer formation of nucleation process.Additionally,the nucleation capacity of SA with amides is greater than that of organic acids with amides. | Chenpeng Zuo Xianwei Zhao Hetong Wang Xiaohui Ma Siyuan Zheng Fei Xu Qingzhu Zhang | 2021 | Journal of Environmental Sciences2021，33，2： | 2 |
| 6 | Proposal and evaluation of a new norm index-based QSAR model to predict pEC50 and pCC50 activities of HEPT derivatives显示文摘anti-HIV 药的搜索和发展当前是药理学研究的最迫切的任务之一。在这个工作,一个量的结构活动关系( QSAR )模型基于某新标准索引,被获得到一系列超过 150 HEPT 衍生物(1-[( 2-hydroxyethoxy )甲基]发现他们的 pEC 50(对病毒的 cytopathic 效果完成 MT-4 房间的50%保护的要求的有效集中)和 pCC 50的-6-(phenylthio)thymine)(减少可见性的要求的细胞毒素的集中50%嘲笑感染了房间)活动。模型效率然后用 leave-one-out 十字确认(LOO-CV ) 和 y 随机化测试被验证。结果显示这个新模型是有效的并且能为 pEC 50 和 pCC 50 的预言向令人满意的结果提供更高的 R 火车 2 和更高的 R 测试 2 。由使用力量途径,这个模型的适用性领域进一步被调查,没有反应孤立点为涉及这个工作的 HEPT 衍生物被检测。有参考方法的比较结果证明这个新方法能为预言 anti-HIV HEPT 衍生物的 pEC 50 和 pCC 50 导致重要改进。而且,在这现在的研究显示出的结果建议 anti-HIV HEPT 衍生物的这二绝对不同的活动 pEC 50 和 pCC 50 能与一个完全类似的 QSAR 模型一起被预言很好,它显示这个模型可能有潜力进一步为 HEPT 衍生物的另外的生物活动被利用。 | Kanwal Shahid Qiang Wang Qingzhu Jia Lei Li Xue Cui Shuqian Xia Peisheng Ma | 2016 | Chinese Journal of Chemical Engineering2016，24，10： | 2 |
| 7 | Preparation and properties of polysulfone/TiO2 composite ultrafiltration membranes显示文摘 | Yanan Yang Peng Wang Qingzhu Zheng | 2006 | Polymer Science Part B: Polymer Physics2006，44，5： | 1 |
| 8 | The relationship between porosity and kinetics parameter of membrane formation in PSF ultrafiltration membrane 显示文摘 | Qingzhu Zheng Peng Wang Yanan Yang | 2006 | Journal of Membrane Science2006，286，12： | 1 |
| 9 | Effect of a thin spreading solvent film on the efficiency of the hexadeeane-l-ol monolayer deposited for water evaporation retardation 显示文摘 | Wu Yan Jia Qingzhu Wang Bingwen Zheng Wei Yi Shouzhi | 2010 | Russian Chemical Bulletin2010，59，9： | 1 |
| 10 | Independentcomponent analysis and its applications in signal process-ing for analytical chemistry 显示文摘 | Wang Guoqing Ding Qingzhu Hou Zhenyu | 2008 | TrAC-Trends in Analyti-cal Chemistry2008，27，： | 1 |
| 11 | Preparation and Characterization of Uniform-sized Chitosan Micro- spheres Containing Insulin by Membrane Emulsification and a Two-step Solidification Process显示文摘 | Wang Lianyan Gu Yonghong Zhou Qingzhu | 2006 | Colloids and Surfaces B: Biointerfaces2006，50，2： | 1 |
| 12 | Integrated evaluation of aerosols from regional brown hazes over northern China in winter: Concentrations, sources, transformation, and mixing states显示文摘 | Li Weijun Zhou Shengzhen Wang Xinfeng Xu Zheng Yuan Chao Yu Yangchun Zhang Qingzhu Wang Wenxing | 2011 | Atmospheres2011，，9： | 1 |
| 13 | Mechanism and rate constants for complete series reactions of 19 fluorophenols with atomic H显示文摘Fluorine-containing halogenated fluorophenol may have efect as intermediate species involved in the formation of polyfluorinated dibenzo-p-dioxin/dibenzofurans(PFDDs/Fs). The mechanism for the atomic H initiated reactions with complete series of nineteen fluorophenol congeners was studies using the density functional theory. At the MPWB1K/6-31+G(d,p) level, the geometries and frequencies of reactants, transition states, and products were obtained, and the accurate energetic values were acquired at the MPWB1K/6-311+G(3df,2p) level. The rate constants were evaluated by the canonical variational transition-state theory with the small curvature tunneling contribution over a wide temperature range of 600–1000 K. The study shows that the intramolecular hydrogen-bond in the ortho-substituted FPs as well as the inductive efect of the electron-withdrawing fluorine and steric repulsion of multiple substitutions may ultimately be responsible for the relative strength of the O–H bonds in FPs. The results can be used for further studies on PFDD/Fs formation mechanism. | Rui Gao Xiaoyan Sun Wanni Yu Qingzhu Zhang Wenxing Wang | 2014 | Journal of Environmental Sciences2014，26，1： | 1 |
| 14 | Preparation and characterization of uniform-sized chitosan microspheres containing insulin by membrane emulsification and a two-step solidification process 显示文摘 | Wang Lianyan Gu Yonghong Zhou Qingzhu | 2006 | Colloids and Surfaces B : Biointerfaces2006，50，： | 1 |
| 15 | Independent component analysis and its applications in signal processing for analytical chemistry 显示文摘 | Wang Guoqing Ding Qingzhu Hou Zhenyu | 2008 | TrAC-Trends in Analytical Chemistry2008，27，36： | 1 |
| 16 | Estimation of source spectra profiles and simultaneous determination of polycomponent in mixtures from ultraviolet spectra data using kernel independent component analysis and support vector regression 显示文摘 | Wang Guoqing Sun Yu'an Ding Qingzhu | 2007 | Analytica Chimica Acta2007，594，1： | 1 |
| 17 | Estimation of source spectra profiles and simultaneous determination of polycomponent in mixtures from ultraviolet spectra data using kernel independent component analysis and support vector regression显示文摘 | Guoqing Wang Yu-an Sun Qingzhu Ding Chunhong Dong Dexue Fu Cunhong Li | 2007 | Analytica Chimica Acta2007，，1： | 1 |
| 18 | Rheological and thermodynamic variation in polysulfone solution by PEG introduction and its effect on kinetics of membrane formation via phaseinversion process显示文摘 | Zheng Qingzhu Wang Peng Yang Yanan | 2006 | J Membr Sci2006，，： | 1 |
| 19 | Size-resolved aerosol ionic composition and secondary formation at Mount Heng in South Central China显示文摘理解解决尺寸的喷雾器在南方华中在上面的边界层影响第二等的喷雾器形成的离子的作文和因素,分离尺寸的喷雾器样品在在山 Heng ( 1269 m asl )的顶点的春天 2009 用微孔的一致存款 impactor ( MOUDI )被收集,由 13 个无机、器官的水溶性的离子的随后的实验室分析列在后面。在 non-dust-storm 时期期间,普通下午 < 潜水艇 class= “ a-plus-plus ” > 1.8 集中是 41.8 g?? | Xinfeng WANG Wenxing WANG Likun XUE Xiaomei GAO Wei NIE Yangchun YU Yang ZHOU Lingxiao YANG Qingzhu ZHANG Tao WANG | 2013 | Frontiers of Environmental Science & Engineering2013，7，6： | 1 |
| 20 | Estimation of source spectra profiles and simultaneous determination of polycomponent in mixtures from ul- traviolet spectra data using kernel independent component analysis and support vector regression 显示文摘 | Guoqing Wang Yu'an Suna Qingzhu Ding Chunhong Dongb Dexue Fub Cunhong Li | 2007 | Analytica Chimica Acta2007，594，4： | 1 |
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