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| 1 | Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle显示文摘Background:Systemic lupus erythematosus(SLE)is a complex autoimmune disease,and the mechanism of SLE is yet to be fully elucidated.The aim of this study was to explore the role of two-pore segment channel 2(TPCN2)in SLE pathogenesis.Methods:Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the expression ofTPCN2 in SLE.We performed a loss-of-function assay by lentiviral construct in Jurkat and THP-1 cell.Knockdown ofTPCN2 were confirmed at the RNA level by qRT-PCR and protein level by Western blotting.Cell Count Kit-8 and flow cytometry were used to analyze the cell proliferation,apoptosis,and cell cycle ofTPCN2-deficient cells.In addition,gene expression profile ofTPCN2-deficient cells was analyzed by RNA sequencing(RNA-seq).Results:TPCN2 knockdown with short hairpin RNA(shRNA)-mediated lentiviruses inhibited cell proliferation,and induced apoptosis and cell-cycle arrest of G2/M phase in both Jurkat and THP-1 cells.We analyzed the transcriptome of knockdown-TPCN2-Jurkat cells,and screened the differential genes,which were enriched for the G2/M checkpoint,complement,and interleukin-6-Janus kinase-signal transducer and activator of transcription pathways,as well as changes in levels of forkhead box O,phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin,and T cell receptor pathways;moreover,TPCN2 significantly influenced cellular processes and biological regulation.Conclusion:TPCN2 might be a potential protective factor against SLE. | Keke Li Jingkai Xu Ke Xue Ruixing Yu Chengxu Li Wenmin Fei Xiaoli Ning Yang Han Ziyi Wang Jun Shu Yong Cui | 2022 | Chinese Medical Journal2022，，4： | 2 |
| 2 | An image matchingrecognition approach based on ICM icon显示文摘 | Ruixing YU Bing ZHU Ke ZHANG | 2008 | Fifth InternationalConference on Fuzzy Systems and Knowledge Discovery2008，4，： | 1 |
| 3 | Evaluation of yield,stability and adaptability of national winter rapeseed regional trials in the upper Yangtze River region in 2017-2018显示文摘Rapeseed variety needs to be tested by regional trial in multiple sites for many years before being applied in market in China.Performants of rapeseed were affected by the interaction of sites and varieties.Evaluation of regional trials is very important for guiding rapeseed breeding.GGE(genotype main effects and genotypeenvironment interaction)biplot was used to evaluate yield,stability,adaptability,representativeness and discrimination of national winter rapeseed trials in the upper reaches of Yangtze River in 2017-2018.Results showed that the main effects of genotype(G),environment(E)and genotypeenvironment interaction(GE)were significant(P<0.01)for yield.Among them,E accounted for 46.95%total variation on average,G and GE accounted for 19.34%and 33.71%respectively.Eight varieties were found with high-yield,excellent stability and adaptability,including‘Yiyou 29’,‘Xiwang 920’,‘Liyouza 108’,‘Nanyou 546’,‘Dadi 195’,‘Jiayou 1’,‘Huayouza 28’and‘Yuhua 2’.Test sites included Nanchong,Mianyang,Wanzhou,Shuangliu and Chengdu in Sichuan Province and Zunyi together with Guiyang in Guizhou Province were selected for their excellent representativeness and discrimination.These results would provide theoretical basis for rapeseed breeding. | Ze’en Yu Lixia Luo Fang Zhang Meiyan Hong Xiangxiang Zhang Ruixing Guo | 2020 | Oil Crop Science2020，5，3： | 1 |
| 4 | Construction of an artificial intelligence system in dermatology: effectiveness and consideration of Chinese Skin Image Database (CSID)显示文摘After more than 60 years of development,artificial intelligence(AI)has been widely used in various fields.Especially in recent years,with the development of deep learning,AI has made many remarkable achievements in the medical field.Dermatology,as a clinical discipline with morphology as its main feature,is particularly suitable for the development of AI.The rapid development of skin imaging technology has helped dermatologists to assist in the diagnosis of diseases and has greatly improved the accuracy of diagnosis.Skin imaging data have natural big data attributes,which is important for AI research.The establishment of the Chinese Skin Image Database(CSID)has solved many problems such as isolated data islands and inconsistent data quality.Based on the CSID,many pioneering achievements have been made in the research and development of AI-assisted decision-making software,the establishment of expert organizations,personnel training,scientific research,and so on.At present,there are still many problems with AI in the field of dermatology,such as clinical validation,medical device licensing,interdisciplinary,and standard formulation,which urgently need to be solved by joint efforts of all parties. | Chengxu Li Wenmin Fei Yang Han Xiaoli Ning Ziyi Wang Keke Li Ke Xue Jingkai Xu Ruixing Yu Rusong Meng Feng Xu Weimin Ma Yong Cui | 2021 | Intelligent Medicine2021，1，2： | 1 |
| 5 | Angiopoietin-like 4 promotes epidermal stem cell proliferation and migration and contributes to cutaneous wound re-epithelialization显示文摘Proliferation and migration of epidermal stem cells(EpSCs)are essential for epithelialization during skin wound healing.Angiopoietin-like 4(ANGPTL4)has been reported to play an important role in wound healing,but the mechanisms involved are not fully understood.Here,we investigate the contribution of ANGPTL4 to full-thickness wound re-epithelialization and the underlying mechanisms using Angptl4-knockout mice.Immunohistochemical staining reveals that ANGPTL4 is significantly upregulated in the basal layer cells of the epidermis around the wound during cutaneous wound healing.ANGPTL4 deficiency impairs wound healing.H&E staining shows that ANGPTL4 deficiency significantly reduces the thickness,length and area of the regenerated epidermis postwounding.Immunohistochemical staining for markers of EpSCs(α6 integrin andβ1 integrin)and cell proliferation(PCNA)shows that the number and proliferation of EpSCs in the basal layer of the epidermis are reduced in ANGPTL4-deficient mice.In vitro studies show that ANGPTL4 deficiency impedes EpSC proliferation,causes cell cycle arrest at the G1 phase and reduces the expressions of cyclins D1 and A2,which can be reversed by ANGPTL4 overexpression.ANGPTL4 deletion suppresses EpSC migration,which is also rescued by ANGPTL4 overexpression.Overexpression of ANGPTL4 in EpSCs accelerates cell proliferation and migration.Collectively,our results indicate that ANGPTL4 promotes EpSC proliferation by upregulating cyclins D1 and A2 expressions and accelerating the cell cycle transition from G1 to S phase and that ANGPTL4 promotes skin wound re-epithelialization by stimulating EpSC proliferation and migration.Our study reveals a novel mechanism underlying EpSC activation and re-epithelialization during cutaneous wound healing. | Yuan Yang Chenghao Yu Yingying Le Weijuan Gong Jihui Ju Guangliang Zhang Pengxiang Ji Rui Zuo Zhe Liu Ping Zhang Ruixing Hou Yi Fu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，8： | 0 |
| 6 | Inhibition of Cancer Cell Migration and Glycolysis by Terahertz Wave Modulation via Altered Chromatin Accessibility显示文摘Metastasis and metabolic disorders contribute to most cancer deaths and are potential drug targets in cancer treatment. However, corresponding drugs inevitably induce myeloid suppression and gastrointestinal toxicity. Here, we report a nonpharmaceutical and noninvasive electromagnetic intervention technique that exhibited long-term inhibition of cancer cells. Firstly, we revealed that optical radiation at the specific wavelength of 3.6 μm (i.e., 83 THz) significantly increased binding affinity between DNA and histone via molecular dynamics simulations, providing a theoretical possibility for THz modulation- (THM-) based cancer cell intervention. Subsequent cell functional assays demonstrated that low-power 3.6 μm THz wave could successfully inhibit cancer cell migration by 50% and reduce glycolysis by 60%. Then, mRNA sequencing and assays for transposase-accessible chromatin using sequencing (ATAC-seq) indicated that low-power THM at 3.6 μm suppressed the genes associated with glycolysis and migration by reducing the chromatin accessibility of certain gene loci. Furthermore, THM at 3.6 μm on HCT-116 cancer cells reduced the liver metastasis by 60% in a metastatic xenograft mouse model by splenic injection, successfully validated the inhibition of cancer cell migration by THM in vivo. Together, this work provides a new paradigm for electromagnetic irradiation-induced epigenetic changes and represents a theoretical basis for possible innovative therapeutic applications of THM as the future of cancer treatments. | Lan Suno Yangmei Li Yun Yu Peiliang Wang Shengquan Zhu KaijieWu Yan Liu Ruixing Wang Li Min Chao Chan | 2022 | Research2022，，4： | 0 |
| 7 | Manipulating the ion-transference and deposition kinetics by regulating the surface chemistry of zinc metal anodes for rechargeable zinc-air batteries显示文摘Aqueous zinc-air battery(ZAB)has attractive features as the potential energy storage system such as high safety,low cost and good environmental compatibility.However,the issue of dendrite growth on zinc metal anodes has seriously hindered the development of ZAB.Herein,the N-doped carbon cloth(NC)prepared via magnetron sputtering is explored as the substrate to induce the uniform nucleation of zinc metal and suppress dendrite growth.Results show that the introduction of heteroatoms accelerates the migration and deposition kinetics of Zn^(2+)by boosting the desolvation process of Zn^(2+),eventually reducing the nucleation overpotential.Besides,theoretical calculation results confirm the zincophilicity of N-containing functional group(such as pyridine N and pyrrole N),which can guide the nucleation and growth of zinc uniformly on the electrode surface by both promoting the redistribution of Zn^(2+) in the vicinity of the surface and enhancing its interaction with zinc atoms.As a result,the half-cell assembled with magnetron sputtered carbon cloth achieves a high zinc stripping/plating coulombic efficiency of 98.8%and long-term stability of over 500 cycles at 0.2 mA cm^(-2).And the Coulombic efficiency reached about 99.5%at the 10th cycle and maintained for more than 210 cycles at a high current density of 5.0 mA cm^(-2).The assembled symmetrical battery can deliver 220 plating/stripping cycles with ultra-low voltage hysteresis of only 11 mV.In addition,the assembled zinc-air full battery with NC-Zn anode delivers a high special capacity of about 429 mAh g_(Zn)^(-1) and a long life of over 430 cycles.The effectiveness of surface functionalization in promoting the transfer and deposition kinetics of Zn^(2+) presented in this work shows enlightening significance in the development of metal anodes in aqueous electrolytes. | Miao He Chaozhu Shu Ruixing Zheng Wei Xiang Anjun Hu Yu Yan Zhiqun Ran Minglu Li Xiaojuan Wen Ting Zeng Jianping Long | 2023 | Green Energy & Environment2023，8，1： | 0 |
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