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| 1 | Distribution of HBV genotypes among HBV carriers in Benin:phylogenetic analysis and virological characteristics of HBV genotype E显示文摘AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant genotype.METHODS: Among 500 blood donors in Benin, 21 HBsAg-positive donors were enrolled in the study. HBV genotypes were determined by enzyme immunoassay and restriction fragment length polymorphism. Complete genome sequences were determined by PCR and direct sequencing.RESULTS: HBV genotype E (HBV/E) was detected in 20/21 (95.2%), and HBV/A in 1/21 (4.8%). From the age-specific prevalence of HBeAg to anti-HBe seroconversion (SC) in 19 HBV/E subjects, SC was estimated to occur frequently in late teens in HBV/E.The comparison of four complete HBV/E genomes from HBeAg-positive subjects in this study and five HBV/E sequences recruited from the database revealed that HBV/E was distributed throughout West Africa with very low genetic divers ity (nucleotide homology 96.7-99.2%).Based on the sequences in the basic core promoter (BCP)to precore region of the nine HBV/E isolates compared to those of the other genotypes, a nucleotide substitution in the BCP, G1757A, was observed in HBV/E.CONCLUSION: HBV/E is predominant in the Republic of Benin, and SC is estimated to occur in late teens in HBV/E. The specific nucleotide substitution G1757A in BCP, which might influence the virological characteristics,is observed in HBV/E. | Kei Fujiwara Yasuhito Tanaka Etsuro Orito Tomoyoshi Ohno Takanobu Kato Kanji Sugihara Izumi Hasegawa Mayumi Sakurai Kiyoaki Ito Atsushi Ozasa Yuko Sakamoto Isao Arita Ahmed El-Gohary Agossou Benoit Sophie I Ogoundele-Akplogan Namiko Yoshihara Ryuzo Ueda Masashi Mizokami | 2005 | World Journal of Gastroenterology2005，11，41： | 3 |
| 2 | Characterization of Trichoderma polysporum from Spitsbergen, Svalbard archipelago, Norway, with species identity, pathogenicity to moss, and polygalacturonase activity显示文摘 | Yusuke Yamazaki Motoaki Tojo Tamotsu Hoshino Kenichi Kida Tatsuji Sakamoto Hideshi Ihara Isao Yumoto Anne Marte Tronsmo Hiroshi Kanda | 2010 | Fungal Ecology2010，，1： | 1 |
| 3 | Optical Trellis-coded modulation with multi- parallel MZM显示文摘 | Takahide Sakamoto Akito Chiba Isao Morohashi Tetsuya Kawanishi | 2009 | ECOC2009，20，2： | 1 |
| 4 | Peritoneal computed tomography attenuation values reflect the severity of peritonitis caused by gastrointestinal perforations显示文摘 | Hironori Tsujimoto Yoshihisa Yaguchi Shuichi Hiraki Naoko Sakamoto Isao Kumano Yusuke Matsumoto Kazumichi Yoshida Hidekazu Sugasawa Satoshi Ono Junji Yamamoto Kazuo Hase | 2011 | The American Journal of Surgery2011，，4： | 1 |
| 5 | Induction of a Pathological Complete Response by Four Courses of Neoadjuvant Chemotherapy for Gastric Cancer: Early Results of the Randomized Phase II COMPASS Trial显示文摘 | Takaki Yoshikawa Kazuaki Tanabe Kazuhiro Nishikawa Yuichi Ito Takanori Matsui Yutaka Kimura Naoki Hirabayashi Shoki Mikata Makoto Iwahashi Ryoji Fukushima Nobuhiro Takiguchi Isao Miyashiro Satoshi Morita Yumi Miyashita Aakira Tsuburaya Junichi Sakamoto | 2014 | Annals of Surgical Oncology2014，，1： | 1 |
| 6 | Induction of a Pathological Complete Response by Four Courses of Neoadjuvant Chemotherapy for Gastric Cancer: Early Results of the Randomized Phase II COMPASS Trial显示文摘 | Takaki Yoshikawa Kazuaki Tanabe Kazuhiro Nishikawa Yuichi Ito Takanori Matsui Yutaka Kimura Naoki Hirabayashi Shoki Mikata Makoto Iwahashi Ryoji Fukushima Nobuhiro Takiguchi Isao Miyashiro Satoshi Morita Yumi Miyashita Aakira Tsuburaya Junichi Sakamoto | 2014 | Annals of Surgical Oncology2014，，1： | 1 |
| 7 | Fabrication of iron disilicide from multilayer by helicon plasma sputtering显示文摘Helicon plasma sputtering of Fe/Si multilayer followed by thermal annealing in Ar atmosphere was used to fabricate β-FeSi2 films. [Fe 0.5nm/Si 1.6nm] 120 multilayer was deposited on Si(100) substrate and then the samples were annealed at 900℃ for 2h or at 9001000℃ for 10s. Annealed samples were characterized by X-ray diffractometry(XRD), Rutherford backscattering spectroscopy, scanning electron microscopy and optical absorption measurement. (202)/(220) oriented β-FeSi2 films are fabricated from the multilayer according to XRD patterns. It is found that there is a very small redistribution of Fe and Si components in the films during the thermal annealing, so that a good stoichiometry of FeSi2 can be obtained. It is also revealed by scanning electron microscopy that multilayer technology can result in a smooth surface for β-FeSi2 films. Optical absorption spectra demonstrate that the films have a direct band gap of 0.9eV at room temperature. | 沈鸿烈 杨颖 SAKAMOTO Isao KOIKE Masaki MAKITA Yunosuke | 2005 | 中国有色金属学会会刊:英文版2005，15，S3： | 0 |
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