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| 1 | Qualitative and quantitative analyses of the bifidobacterial microbiota in the colonic mucosa of patients with colorectal cancer, diverticulitis and inflammatory bowel disease显示文摘AIM: To characterize the bifidobacterial microbiota of the colonic mucosa in patients with colon cancer, inflammatory bowel disease or diverticulitis. METHODS: A sample of the distal colonic mucosa was taken during surgery from a total of 34 patients, twenty-one with diagnosed colorectal cancer, nine with diverticulitis and four with inflammatory bowel disease, requiring surgery for their condition. Bacterial DNA was extracted from the resected mucosal samples and bifidobacterial mucosa-associated microbiota was qualitatively and quantitatively determined by means of qualitative and quantitative PCR. RESULTS: Bifidobacteria were found in 100% of the samples from patients with diverticulitis or IBD and a 76% of those suffering colon cancer. The species B. longum and B. bifidum were the most widely found, followed by B. animalis, B. catenulatum and B. adolescentis. B. breve, B. dentium and B. angulatum were not detected in any sample. A significantly higher occurrence of B. longum was observed in patients with diverticulitis than in those with colon cancer or IBD (100%, 62% and 75%, respectively, P < 0.05). Similar results were obtained for B. animalis (56%, 0% and 25%, P < 0.05), while B. adolescentis was only found in the mucosa from patients with colon cancer (5 out of 21, 24%). At the quantitative level, patients with colon cancer or IBD showed lower counts of total Bifidobacterium (4.94 and 5.91 vs 6.96 log Cells/sample, respectively, P < 0.05) and of the species B. longum (4.05 and 4.79 vs 6.76, P < 0.05) than those with diverticulitis. CONCLUSION: Aberrancies in mucosa associated microbiota are present in different intestinal diseases. This may indicate a role of the microbiota in the pathogenesis of these diseases. | Miguel Gueimonde Arthur Ouwehand Heikki Huhtinen Eeva Salminen Seppo Salminen | 2007 | World Journal of Gastroenterology2007，13，29： | 25 |
| 2 | Specific probiotics alleviate allergic rhinitis during the birch pollen season显示文摘AIM:To investigate whether birch pollen allergy symptoms are linked with gut microbiota changes and whether probiotics have an effect on these.METHODS:Forty seven children with confirmed birch pollen allergy were randomized to receive either a probiotic combination of Lactobacillus acidophilus (L.acidophilus) NCFMTM (ATCC 700396) and Bifidobacterium lactis (B.lactis) Bl-04 (ATCC SD5219) or placebo in a double-blind manner for 4 mo,starting prior to onset of the birch pollen season.Symptoms were recorded in a diary.Blood samples were taken for analysis of cytokines and eosinophils.Fecal samples were analysed for microbiota components,calprotectin and IgA.Nasal swabs were taken for analysis of eosinophils.RESULTS:The pollen season induced a reduction in Bifidobacterium,Clostridium and Bacteroides which could not be prevented by the probiotic intervention.During the intervention,significantly higher numbers of B.lactis 11.2×107 ± 4.2×107 vs 0.1×107 ± 0.1×107 bacteria/g feces (P<0.0001) and L.acidophilus NCFMTM 3.5×106 ± 1.3×106 vs 0.2×106 ± 0.1×106 bacteria/g feces (P<0.0001) were observed in the probiotic group compared to the placebo group.During May,there was a tendency for fewer subjects,(76.2% vs 95.2%,P=0.078) to report runny nose,while during June,fewer subjects,11.1% vs 33.3%,reported nasal blocking in the probiotics group (P =0.101).Concomitantly,fewer subjects in the probiotic group had infiltration of eosinophils in the nasal mucosa compared to the placebo group,57.1% vs 95% (P=0.013).Eye symptoms tended to be slightly more frequent in the probiotic group,12.5 d [interquartile range (IQR) 6-18] vs 7.5 d (IQR 0-11.5) (P=0.066) during May.Fecal IgA was increased in the placebo group during the pollen season;this increase was prevented by the probiotics (P=0.028).CONCLUSION:Birch pollen allergy was shown to be associated with changes in fecal microbiota composition.The specific combination of probiotics used was shown to prevent the pollen-induced infiltration of eosinophils into the nasal mucosa,and indicated a trend for reduced nasal symptoms. | Arthur C Ouwehand Merja Nermes Maria Carmen Collado Nina Rautonen Seppo Salminen Erika Isolauri | 2009 | World Journal of Gastroenterology2009，15，26： | 19 |
| 3 | Evolutionary History of the Non-Specific Lipid Transfer Proteins显示文摘非特定的类脂化合物转移蛋白质(nsLTPs ) 小、基本蛋白质由一个像隧道的恐水病的洞被描绘,能够转移在类脂化合物 bilayers 之间的各种各样的类脂化合物分子。大多数 nsLTPs 与本地化蛋白质到 apoplastic 空格的 N 终端信号肽被综合。nsLTPs 仅仅在种子植物被识别了,在他们被大基因家庭编码的地方。我们从非种子陆地植物和绿水藻用 genomic 和 EST 信息开始了 nsLTP 家庭的进化历史的分析决定:(1 ) 当 nsLTP 家庭产生了时,(2 ) 经常新的 nsLTP 亚科怎么被创造了,并且(3 ) 亚科怎么在不同植物系在他们扩大和损失的模式不同。在这研究,我们寻找了顺序数据库并且发现编码 nsLTPs 的基因和抄本充满地钱,苔藓,和另外的调查陆地种的所有,然而并非在任何水藻介绍。代表性的地钱和苔藓 nsLTPs 的第三级的结构进一步与相同建模被学习。在植物征服了土地以后,结果显示 nsLTP 家庭演变。仅仅, nsLTPs 的四个主要亚科中的二个在 flowering 植物发现了在苔藓和地钱是在场的。另外的亚科以后产生了,在陆地植物进化期间。在这份报告,我们也介绍一个修改 nsLTP 分类系统。 | Monika M. Edstam Lenita Viitanen Tiina A. Salminen Johan Edqvist | 2011 | Molecular Plant2011，4，6： | 18 |
| 4 | Increased proliferation activity measured by immunoreactive Ki67 is associated with survival improvement in rectal/recto sigmoid cancer显示文摘AIM: To assess the expression of Ki67 as prognosticator in rectal/recto sigmoid cancer.METHODS: Samples from 146 patients with rectal and recto sigmoid cancer were studied for expression of Ki67 and its prognostic significance in comparison with clinicopathological predictors of survival. Formalin-fixed, paraffin-embedded tissues from 6 (4.1%) patients with T1, 26 (17.8%) with T2, 94 (64.4%) with T3, and 20 (13.7%) with T4 tumors were studied. Ki67 expression was determined immunohistochemically. Samples were divided according to mean value into high (>40%) and low (≤40%) expression. Areas of extensive proliferation (>50%) were defined as 'hot spot' areas. RESULTS: Hot spot areas were present in samples regardless of histopathological grade. Lower TNM and Dukes stage and higher expression of Ki67 and presence of Ki67 hot spot areas in histopathological samples were associated with better survival, whereas no association was observed with histopathological grade (P = 0.78). In Cox multivariate regression analysis, significant prognostic factors were Dukes stage (P<0.001), presence of lymph node metastases (P = 0.015), age (P = 0.035) andpresence of Ki67 hot spot areas (P = 0.044). CONCLUSION: Proliferative activity as measured by Ki67 in rectal cancer is associated with survival improvement compared with patients with low Ki67. Areas of prognostically significant increased proliferation were found independently of histopathological tumor grade. | Eeva Salminen Salla Palmu Tero Vahlberg Peter J.Roberts Karl-Owe S(o|¨)derstr(o|¨)m | 2005 | World Journal of Gastroenterology2005，11，21： | 15 |
| 5 | Fecal Transplantation, Through Colonoscopy, Is Effective Therapy for Recurrent Clostridium difficile Infection显示文摘 | Eero Mattila Raija Uusitalo–Sepp?l? Maarit Wuorela Laura Lehtola Heimo Nurmi Matti Ristikankare Veikko Moilanen Kimmo Salminen Maaria Sepp?l? Petri S. Mattila Veli–Jukka Anttila Perttu Arkkila | 2012 | Gastroenterology2012，，3： | 3 |
| 6 | Relationship between COX-2 and cell cycle-regulatory proteins in patients with esophageal squamous cell carcinoma显示文摘AIM: To investigate the correlation between cyclooxygenase-2 (COX-2) and cell cycle-regulatory proteins in patients with esophageal squamous cell carcinoma (ESCC). METHODS: One hundred and two surgically obtained specimens of ESCC were randomly collected. All specimens were obtained from patients who had not received chemoor radiotherapy prior to surgical resection.Twenty-eight specimens of normal squamous epithelium served as controls. The expression of COX-2, Ki-67, cyclin A and p27 was examined by immunohistochemistry. The Pearson test was used to analyze the relationship between groups. RESULTS: The protein level of COX-2, Ki-67 and cyclin A was significantly higher in ESCC than in normal squamous epithelium (74.7±61.2 vs 30.2 ± 43.4, 64.0 ± 51.6 vs 11.6 ± 2.3, 44.2 ± 32.2 vs 11.7 ± 5.0, respectively, all P<0.01). In contrast, the protein level of p27 was signifi cantly lower in ESCC than in normal squamous epithelium (182.0 ±69.0 vs 266.4±28.0, P<0.01). In ESCC, COX-2 expression was correlated with T stage, the score of T1-T2 stage was lower than that of T3-T4 stage (55.0±42.3 vs 83.0 ± 66.5, P<0.05), and Ki-67, cyclin A and p27 expressions were correlated with the tumor differentiation (43.8±31.7 vs 98.4± 84.8, 32.0 ± 19.0 vs 54.1 ±53.7,206.2±61.5 vs 123.5±68.3, respectively, all P<0.01). COX-2 expression was positively correlated to Ki-67, cyclin A and negatively correlated to p27 expression in ESCC (r=0.270, 0.233 and-0.311, respectively, all P<0.05).CONCLUSION: The expression of COX-2 is correlated with tumor cell invasion and is closely related to the cell proliferation in patients with ESCC. | Jun-Xing Huang Mao-Song Lin Yun-Lei Zhang Feng-Yue Li Rong-Yu Qian Wei-Chang Chen Zheng-Xiang Song Ping Chen Eeva Salminen | 2010 | World Journal of Gastroenterology2010，16，47： | 3 |
| 7 | Expression of thymidylate synthase and glutathione-stransferase π in patients with esophageal squamous cell carcinoma显示文摘AIM:To investigate the expression of thymidylate synthase(TS)and glutathione-s-transferaseπ(GST-π) in esophageal squamous cell carcinoma and their association with the clinicopathologic characteristics. METHODS:Immunohistochemical methods were used to detect the expression of TS and GST-πin surgically resected formalin-fixed,paraffin-embedded esophageal squamous cell carcinoma(ESCC)tissue sections from 102 patients(median age,58 years)and in 28 normal esophageal mucosa(NEM)samples.The relationship between TS and GST-πexpression and clinicopathologic factors was examined. RESULTS:The expression of TS and GST-πwas not statistically significantly associated with age of the patients,tumor size,lymph node metastasis,depth of invasion or tumor stage.TS staining was positive in 17.86%of normal esophageal mucosa and in 42.16% of ESCC samples(P<0.05).The expression level of TSwas not only significantly lower in well-differentiated (21.88%)than in poorly-differentiated carcinomas (51.43%,P<0.05),but was also significantly higher in samples from male patients(46.51%)than from female patients(18.75%,P<0.05).GST-πwas positively stained in 78.57%of normal esophageal mucosa and in 53.92%of ESCC samples(P<0.05).The expression level of GST-πwas also significantly higher in welldifferentiated carcinomas(65.63%)than in poorlydifferentiated carcinomas(35.00%,P<0.05). CONCLUSION:The expression of TS and of GST-πmay be used as molecular markers for the characterization of ESCC.Poorly-differentiated cells showed increased expression of TS and reduced expression of GST-π. | Jun-Xing Huang Feng-Yue Li Wei Xiao Zheng-Xiang Song Rong-Yu Qian Ping Chen, Eeva Salminen | 2009 | World Journal of Gastroenterology2009，15，34： | 3 |
| 8 | Faecal microbiota transplantation in patients with Clostridium difficile and significant comorbidities as well as in patients with new indications:A case series显示文摘Fecal microbiota transplantation(FMT) is effective in recurrent Clostridium difficile infection(r CDI). Knowledge of the safety and efficacy of FMT treatment in immune deficient patients is scarce. FMT has been suggested as a potential method for an increasing number of new indications besides r CDI. Among our FMT-treated r CDI patients, we reviewed those with major comorbidities: two human immunodeficiency virus patients, six haemodialysis patients, two kidney transplant patients, two liver transplant patients and a patient with chronic lymphatic leukaemia. We also reviewed those treated with FMT for indications other than r CDI: Salmonella carriage(two patients), trimethylaminuria(two patients), small intestinal bacterial overgrowth(SIBO;one patient), and lymphocytic colitis(one patient), as well as a common variable immunodeficiency patient with chronic norovirus infection and ESBL-producing Escherichia coli(E. coli) carriage. Of the thirteen r CDI patients treated with FMT, eleven cleared the CDI. The observed adverse events were not directly attributable to FMT. Concerning the special indications, both Salmonellas and ESBL-producing E. coli were eradicated. One trimethylaminuria patient and one SIBO-patient reported a reduction of symptoms. Three patients did not experience a benefit from FMT: chronic norovirus, lymphocytic colitis and the other fish malodour syndrome. There were no reported side effects in this group. FMT appeared to be safe and effective for immunocompromised patients with r CDI. FMT showed promise for the eradication of antibiotic-resistant bacteria, but further research is warranted. | Perttu Lahtinen Eero Mattila Veli-Jukka Anttila Jyrki Tillonen Matti Teittinen Pasi Nevalainen Seppo Salminen Reetta Satokari Perttu Arkkila | 2017 | World Journal of Gastroenterology2017，23，39： | 2 |
| 9 | Severe and fatal complications after ERCP: Analysis of 2555 procedures in a single experienced center显示文摘 | P. Salminen S. Laine R. Gullichsen | 2008 | Surgical Endoscopy2008，，9： | 2 |
| 10 | Inflammation in Alzheimer’s disease: Amyloid-β oligomers trigger innate immunity defence via pattern recognition receptors显示文摘 | Antero Salminen Johanna Ojala Anu Kauppinen Kai Kaarniranta Tiina Suuronen | 2009 | Progress in Neurobiology2009，，3： | 2 |
| 11 | Bile duct injuries during laparoscopic cholecystectomy: primary and long-term results from a single institution显示文摘 | Jukka Karvonen Risto Gullichsen Simo Laine Paulina Salminen Juha M. Gr?nroos | 2007 | Surgical Endoscopy2007，，7： | 2 |
| 12 | Inflammation in Alzheimer’s disease: Amyloid-β oligomers trigger innate immunity defence via pattern recognition receptors显示文摘 | Antero Salminen Johanna Ojala Anu Kauppinen Kai Kaarniranta Tiina Suuronen | 2009 | Progress in Neurobiology2009，，3： | 2 |
| 13 | Host Remodeling of the Gut Microbiome and Metabolic Changes during Pregnancy显示文摘 | Omry Koren Julia K. Goodrich Tyler C. Cullender Aymé Spor Kirsi Laitinen Helene Kling B?ckhed Antonio Gonzalez Jeffrey J. Werner Largus T. Angenent Rob Knight Fredrik B?ckhed Erika Isolauri Seppo Salminen Ruth E. Ley | 2012 | Cell2012，，3： | 2 |
| 14 | Influence of mode of delivery on gut microbiota composition in seven years old children显示文摘 | Salminen S Gibson GR McCaetney AL | 2004 | Gut2004，53，9： | 1 |
| 15 | The role of the intestinal microflora for the development of the immune system in early childhood显示文摘 | Isolauri E Salminen S | 2002 | Eur J Nutr2002，411，： | 1 |
| 16 | Quaternary structure and metal ion requirement of family Ⅱ pyrophosphatases from Bacillus subtilis, Streptococcus gordonii, and Streptococcus rnutans 显示文摘 | Parfenyev A N Salminen A Halonen P | 2001 | J Biol Chem2001，276，24： | 1 |
| 17 | Tissue response to polyglycolide,polydioxanone,polylevolactide,and metallic pins in cancellous bone:An experimental study on rabbits显示文摘 | Pihlajamaki H Salminen S Laitinen O | 2006 | J Orthop Res2006，24，： | 1 |
| 18 | Specific probiotics in reducing the risk of acute infections in infancy - a randomized, double-blind, placebo-controlled study显示文摘 | SAMULI RAUTAVA SEPPO SALMINEN | 2009 | British Journal of Nutrition2009，，101： | 1 |
| 19 | Nurse educator competence : A study of Norwegian nurse educators' opinions of the importance and application of different nurse educator competence domains 显示文摘 | Johnson KO Aasgaard HS Wahl AK Salminen L | 2002 | Journal of Nursing Education2002，41，7： | 1 |
| 20 | Considerations regarding the definition of a geochemicjal baseline of elements in the surfical materials in areas differing in basic geology显示文摘 | Salminen R Gregorauskiene V | 2000 | Applied Geochemistry2000，15，： | 1 |
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