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| 1 | Incidence and mortality of laryngeal cancer in China, 2011显示文摘Objective: Laryngeal cancer is the common cancer of the upper aerodigestive tract. We aimed to use the national cancer registration data in 2011 to estimate the incidence and mortality of laryngeal cancer within China.Methods: Comparable, high-quality data from 177 population-based cancer registries were qualified for analysis. The pooled data were stratified by area, sex and age group. National new cases and deaths of laryngeal cancer were estimated using age-specific rates and national population in 2010. All incidence and death rates were age-standardized to the 2000 Chinese standard population and Segi's population, which were expressed per 100,000 populations.Results: All 177 cancer registries covered a total of 175,310,169 population(98,341,507 in urban and 76,968,662 in rural areas), accounting for 13.01% of the national population. The data quality indicators of proportion of morphological verification(MV%), percentage of cancer cases identified with death certification only(DCO%) and mortality to incidence ratio(M/I) were 77.98%, 2.62% and 0.55, respectively. Estimated 20,875 new cases of laryngeal cancer were diagnosed and 11,488 deaths from laryngeal cancer occurred in China in 2011. The crude incidence rate of laryngeal cancer was 1.55/100,000(2.69/100,000 in males and 0.35/100,000 in females). Age-standardized incidence rates by Chinese standard population(ASIRC) and by world standard population(ASIRW) were 1.13/100,000 and 1.14/100,000, respectively. Laryngeal cancer is much rarer in females than in males. The incidence rate was higher in urban areas than that in rural areas. The crude mortality rate of laryngeal cancer was 0.85/100,000(1.42/100,000 in males and 0.25/100,000 in females). Age-standardized mortality rates by Chinese standard population(ASMRC) and by world standard population(ASMRW) were both 0.61/100,000. The mortality rate in males was much higher than that in females. There was no definite difference in mortality rates of laryngeal cancer between urban and rural areas.Conclusions: Larynx is a specialized area and cancer of larynx significantly affects the quality of life for the patients. Comprehensive measures should be carried out to prevent the ascent of laryngeal cancer. | Lingbin Du Huizhang Li Chen Zhu Rongshou Zheng Siwei Zhang Wanqing Chen | 2015 | Chinese Journal of Cancer Research2015，27，1： | 29 |
| 2 | Annual cost of illness of stomach and esophageal cancer patientsin urban and rural areas in China: A multi-center study显示文摘Objective: Stomach and esophageal cancer are imposing huge threats to the health of Chinese people whereas there were few studies on the financial burden of the two cancers.Methods: Costs per hospitalization of all patients with stomach or esophageal cancer discharged between September 2015 and August 2016 in seven cities/counties in China were collected, together with their demographic information and clinical details. Former patients in the same hospitals were sampled to collect information on annual direct non-medical cost, indirect costs and annual number of hospitalization. Annual direct medical cost was obtained by multiplying cost per hospitalization by annual number of hospitalization. Annual cost of illness(ACI)was obtained by adding the average value of annual direct medical cost, direct non-medical cost and indirect cost,stratified by sex, age, clinical stage, therapy and pathologic type in urban and rural areas. Costs per hospitalization were itemized into eight parts to calculate the proportion of each part. All costs were converted to 2016 US dollars(1 USD=6.6423 RMB).Results: Totally 19,986 cases were included, predominately male. Mean ages of stomach cancer and urban patients were lower than that of esophageal cancer and rural patients. ACI of stomach and esophageal cancer patients were $10,449 and $13,029 in urban areas, and $2,927 and $3,504 in rural areas, respectively. Greater ACI was associated with male, non-elderly patients as well as those who were in stage I and underwent surgeries.Western medicine fee took the largest proportion of cost per hospitalization.Conclusions: The ACI of stomach and esophageal cancer was tremendous and varied substantially among the population in China. Preferential policies of medical insurance should be designed to tackle with this burden and further reduce the health care inequalities. | Zhixun Yang Hongmei Zeng Ruyi Xia Qian Liu Kexin Sun Rongshou Zheng Siwei Zhang Changfa Xia He Li Shuzheng Liu Zhiyi Zhang Yuqin Liu Guizhou Guo Guohui Song Yigong Zhu Xianghong Wu Bingbing Song Xianzhen Liao Yanfang Chen Wenqiang Wei Guihua Zhuang Wanqing Chen | 2018 | Chinese Journal of Cancer Research2018，30，4： | 22 |
| 3 | Optical beam steering by using tunable,narrow-linewidth butt-coupled hybrid lasers in a silicon nitride photonics platform显示文摘Chip-scale,tunable narrow-linewidth hybrid integrated diode lasers based on quantum-dot RSOAs at 1.3μm are demonstrated through butt-coupling to a silicon nitride photonic integrated circuit.The hybrid laser linewidth is around 85 k Hz,and the tuning range is around 47 nm.Then,a fully integrated beam steerer is demonstrated by combining the tunable diode laser with a waveguide surface grating.Our system can provide beam steering of 4.1°in one direction by tuning the wavelength of the hybrid laser.Besides,a wavelength-tunable triple-band hybrid laser system working at^1,~1.3,and^1.55μm bands is demonstrated for wide-angle beam steering in a single chip. | YEYU ZHU SIWEI ZENG LIN ZHU | 2020 | Photonics Research2020，8，3： | 4 |
| 4 | Dual functional transparent film for proximity and pressure sensing显示文摘在过去几年,触觉的察觉到的技术的快速的开发显著地作出贡献到直觉的触摸控制和聪明的人机器的相互作用的实现。除了察觉到的物理触觉或压力,最近作为互补功能察觉到能扩大普通单个功能的触觉的传感器的察觉模式。在这个工作,我们在场一透明,矩阵结构集成在一台设备察觉到的最近和压力的能力的双功能的电容的传感器,并且电容的象素的孤立的反应提供的优秀空间分辨率使我们能认识到与快反应,高稳定性和高可逆性接近对象和装载压力的精确地点鉴定。 | Bo Zhang Zemin Xiang Siwei Zhu Qiyi Hu Yuanzhi Cao Junwen Zhong Qize Zhong Bo Wang Yunsheng Fang Bin Hu Jun Zhou Zhonglin wang | 2014 | Nano Research2014，7，10： | 4 |
| 5 | Practice and understanding of developing new technologies and equipment for green and low-carbon production of oilfields显示文摘The core of China’s low-carbon development includes optimization of industrial structure,clean energy technologies,emission reduction technologies,and innovation of relevant systems and institutions.China National Petroleum Corporation(CNPC)has always been a proactive participant in developing low-carbon economy,shouldering the responsibilities of safeguarding oil and gas supply,conserving energy,and reducing emission.Therefore,CNPC fulfills those responsibilities as a substantial part of its overall strategy.Guided by low carbon and driven by innovation,petroleum corporations have taken constant innovation of low-carbon technologies,especially the development of green and low-carbon petroleum engineering technologies and equipment,as major measures for energy conservation and emission reduction.Large-scale development mode o f unconventional resource anhydrous fracturing should be innovated.And supercritical C 02 should be used to replace water for fracturing operation,in order to achieve multiple objectives of C 02 burying,conserve water resource,improve single well production and ultimate recovery,realizing reduced emission and efficient utilization o f C 02 resources.Artificial lifting energy-saving and efficiencyincreasing technologies and injection-production technology in the same well should also be innovated.Energy consumption of high water-cut wells is reduced to support the new low-carbon operation mode of high water-cut oilfields and realize energy saving and efficiency improvement during oil production by developing the operation efficiency of the lifting system and reducing the ineffective lifting of formation water.These technologies have been widely recognized by local and international experts and have greatly enhanced CNPC's international influence.This study expounds the key technologies and equipment with regard to the development of green and low-carbon petroleum engineering and provide relevant suggestions. | He LIU Jianwen YAN Siwei MENG Qinghai YANG Zixiu YAO Shijia ZHU | 2019 | Frontiers of Engineering Management2019，6，4： | 3 |
| 6 | Rare earth doping effects on superconducting properties of MgB_2:A review显示文摘Rare earth(RE) elements and their compounds have been doped into MgB_2 since the discovery of the superconductivity of MgB_2. The doping effects of RE elements and RE compounds are summarized in terms of different doping methods and the accompanying variations in the superconducting transition temperature, upper critical field, irreversibility field, and critical current density(Jc). Conclusions are drawn on the doping mechanisms to demonstrate the possibility of improvement of Jcin the future. REBy phase inclusions are observed in MgB_2 as nano-pinning centers with significant enhancement of Jcin MgB_2 doped with Y, La, Dy, Ho, Nd, etc. Furthermore, some REs with larger magnetic moments will provide more efficient flux pinning centers with larger pinning forces, which is different with the doping effects and mechanism of ferromagnetic transition metals in MgB_2. The co-doping effects of RE with other dopants are also reviewed due to the multiple advantages of different doping mechanisms. | Wenxian Li Jixiang Kang Siwei Fu Yemin Hu Pengfei Hu Mingyuan Zhu Ying Li | 2019 | Journal of Rare Earths2019，37，2： | 2 |
| 7 | Wide-field in situ multiplexed Raman imaging with superresolution显示文摘Because of the fingerprint-like specificity of its characteristic spectrogram, Raman spectral imaging has been applied widely in various research areas. Using a combination of structured illumination with the surface-enhanced Raman scattering(SERS) technique, wide-field Raman imaging is developed with a significant improvement in spatial resolution. As a result of the relatively narrow Raman characteristic peaks, optically encoded SERS nanoparticles can be used to perform multiplexed imaging. The results show excellent superresolution wide-field multiplexed imaging performance. The developed technique has extraordinary potential for applications in biological imaging and other related fields. | HOUKAI CHEN XIAOJING WU YUQUAN ZHANG YONG YANG CHANGJUN MIN SIWEI ZHU XIAOCONG YUAN QIAOLIANG BAO JING BU | 2018 | Photonics Research2018，6，6： | 2 |
| 8 | Meiotic nuclear divisions 1(MND1)fuels cell cycle progression by activating a KLF6/E2F1 positive feedback loop in lung adenocarcinoma显示文摘Background:Considering the increase in the proportion of lung adenocarcinoma(LUAD)cases among all lung cancers and its considerable contribution to cancer-related deaths worldwide,we sought to identify novel oncogenes to provide potential targets and facilitate a better understanding of the malignant progression of LUAD.Methods:The results from the screening of transcriptome and survival analyses according to the integrated Gene Expression Omnibus(GEO)datasets and The Cancer Genome Atlas(TCGA)data were combined,and a promising risk biomarker called meiotic nuclear divisions 1(MND1)was selectively acquired.Cell viability assays and subcutaneous xenograftmodelswere used to validate the oncogenic role ofMND1 in LUADcell proliferation and tumor growth.Aseries of assays,including mass spectrometry,co-immunoprecipitation(Co-IP),and chromatin immunoprecipitation(ChIP),were performed to explore the underlying mechanism.Results:MND1 up-regulation was identified to be an independent risk factor for overall survival in LUAD patients evaluated by both tissue microarray staining and third party data analysis.In vivo and in vitro assays showed that MND1 promoted LUAD cell proliferation by regulating cell cycle.The results of the Co-IP,ChIP and dual-luciferase reporter assays validated that MND1 competitively bound to tumor suppressor Kruppel-like factor 6(KLF6),and thereby protecting E2F transcription factor 1(E2F1)from KLF6-induced transcriptional repression.Luciferase reporter and ChIP assays found that E2F1 activated MND1 transcription by binding to its promoter in a feedback manner.Conclusions:MND1,KLF6,and E2F1 form a positive feedback loop to regulate cell cycle and confer DDP resistance in LUAD.MND1 is crucial for malignant progression and may be a potential therapeutic target in LUAD patients. | Quanli Zhang Run Shi Yongkang Bai Lijuan Meng Jingwen Hu Hongyu Zhu Tongyan Liu Xiaomeng De Siwei Wang Jie Wang Lin Xu Guoren Zhou Rong Yin | 2021 | Cancer Communications2021，41，6： | 2 |
| 9 | An analysis of major scientific problems and research paths of Gulong shale oil in Daqing Oilfield, NE China显示文摘After the preliminary basic research on the problems encountered during the production period of Gulong shale oil in the Songliao Basin, NE China, and the scientific exploration, the special characteristics of Gulong shale oil in terms of reservoir space, phase distribution, flow pattern, and mineral evolution are proposed. The main results are as follows :(1) The source of organic matter, mechanism of hydrocarbon generation and expulsion, and key factors affecting shale oil abundance;(2) The types and structural characteristics of the reservoir and their contribution to porosity and permeability;(3) The mineral origin and evolution of minerals and their influence on reservoir availability, sensitivity, and compressibility;(4) The rock mechanical characteristics and fracture propagation law of Gulong shale;(5) The shale oil products, phase change law and main control factors of adsorption and desorption conversion of Gulong shale oil;(6) The mechanism of shale oil-liquid, solid-liquid gas interaction and enhanced oil recovery. Three key research suggestions are proposed to realize the large-scale economic utilization of the Gulong shale oil as follows:(1) Deepen research on the mechanism of oil and gas generation and discharge, storage and transportation, to guide the selection of geological sweet spots of shale oil;(2) Deepen research on the compressibility and fracture initiation mechanism to support the selection of engineering sweet spots and optimization of engineering design;(3) Deepen research on the fluid interaction mechanism under reservoir conditions, os us to guide the optimization of development schemes and the selection of EOR technologies. A successful development of Gulong shale oil requires global experts and scholars to contribute multidisciplinary innovative ideas and technical ideas to solve production problems. | SUN Longde LIU He HE Wenyuan LI Guoxin ZHANG Shuichang ZHU Rukai JIN Xu MENG Siwei JIANG Hang | 2021 | Petroleum Exploration and Development2021，48，3： | 2 |
| 10 | LncRNA LINC00525 suppresses p21 expression viamRNA decay and triplex-mediated changes in chromatin structure in lung adenocarcinoma显示文摘Background:Emerging evidence suggests that long noncoding RNAs(lncRNAs)play crucial roles in various cancers.In the present study,we aim to investigate the function and molecular mechanism of an up-regulated and survivalassociated lncRNA,LINC00525,in lung adenocarcinoma(LUAD).Methods:The expression level of LINC00525 in tissueswas determined by quantitative reverse transcription polymerase chain reaction(RT-qPCR)and in situ hybridization(ISH).The functional role of LINC00525 in LUAD was investigated using gain-and loss-of-function approaches,both in vivo and in vitro.RNA pull-down,RNA immunoprecipitation(RIP),chromatin immunoprecipitation(ChIP),triplex-capture assay,dual-luciferase assay,gene expression microarray,and bioinformatics analysis were used to investigate the potential underlying mechanisms involved.Results:LINC00525 is highly expressed in LUAD cells and tissues.Survival analysis indicated that upregulation of LINC00525 was associated with poor prognosis in patients with LUAD patients.Knockdown of LINC00525 inhibited cell proliferation and cell cycle progression in vitro.In xenograft models,LINC00525 knockdown suppressed tumor growth and tumorigenesis of tumorbearing mice.Mechanistically,LINC00525 epigenetically suppressed p21 transcription by guiding Enhancer Of Zeste 2 Polycomb Repressive Complex 2 Subunit(EZH2)to the p21 promoter through an formation of RNA-DNA triplex with the p21 promoter,leading to increased trimethylation of lysine 27 on histone 3(H3K27me3)of the p21 promoter.In addition,LINC00525 repressed p21 expression post-transcriptionally by enhancing p21mRNA decay.LINC00525 promoted p21mRNAdecay by competitively binding toRNABindingMotif Single Stranded Interacting Protein 2(RBMS2).Conclusion:Our findings demonstrate that LINC00525 promotes the progression of LUAD by reducing the transcription and stability of p21 mRNA in concert with EZH2 and RBMS2,thus suggesting that LINC00525 may be a potential therapeutic target for clinical intervention in LUAD. | Panqi Fang Hao Chen Zhifei Ma Chencheng Han Wenda Yin Siwei Wang Hongyu Zhu Wenjia Xia Jie Wang Lin Xu Tongyan Liu Rong Yin | 2021 | Cancer Communications2021，41，7： | 2 |
| 11 | Comparison of Three Microwave/Chemical Pretreatment Processes for Enzymatic Hydrolysis of Rice Straw显示文摘 | Shengdong Zhu Yuanxin Wu Ziniu Yu Cunwen Wang Faquan Yu Siwei Jin Yigang Ding Ru’an Chi Jintao Liao Yan Zhang | 2005 | Biosystems Engineering2005，，3： | 1 |
| 12 | Associationbe- tweenpl6 INK4apromoter methylation and non-small cell- lungcancer: ameta - analysis 显示文摘 | Jundong Gu Yanjun wen Siwei zhu | 2013 | PLoS One2013，8，60： | 1 |
| 13 | Conservation of weathered historic sandstone with biomimetic apatite显示文摘The conservation of weathered historic sandstone with apatite was studied.Based on the growth mechanism of bone,calcium and phosphorus were introduced into weathered sandstone and then mineralized at room temperature.The conservation efficiency was investigated by scanning electron microscopy(SEM),energy-dispersive X-ray spectroscopy(EDX),transmission electron microscopy(TEM),selected area electron diffraction(SAED),X-ray diffraction(XRD),and compressive strength,capillary water uptake,water vapor permeability and weather resistance tests.The SEM,EDX,TEM,SAED and XRD results showed that the produced hydroxyapatite could reunite the weathered stone blocks and provide sufficient reinforcement to hold them together.The results of the various tests indicated that the compressive strength and weather resistance of the treated samples was improved significantly.In addition,because of the porous nature of apatite,the water vapor permeability of the treated stone was not affected and its breathability was maintained. | YANG FuWei LIU Yan ZHU YuanCheng LONG ShiJia ZUO GuoFang WANG ChangQing GUO Feng ZHANG BingJian JIANG SiWei | 2012 | Chinese Science Bulletin2012，57，17： | 1 |
| 14 | Retrieved lymph nodes from different anatomic groups in gastric cancer:a proposed optimal number,comparison with other nodal classification strategies and its impact on prognosis显示文摘Background:The optimal number of retrieved lymph nodes(LNs)in gastric cancer(GC)is still debatable and previ-ous studies proposing new classification alternatives mostly focused on the number of retrieved LNs without proper consideration on the anatomic nodal groups’location.Here,we assessed the impact of retrieved LNs from different nodal location groups on the survival of GC patients.Methods:Stage I-III gastric cancer patients who had radical gastrectomy were investigated.LN grouping was deter-mined according to the 13th edition of the JCGC.The optimal cut-off values of retrieved LNs in different LN groups(Group 1 and 2)were calculated,based on which a proposed nodal classification(rN)simultaneously accounting the optimal number and location of retrieved LNs was proposed.The performance of rN was then compared to that of LN ratio,log-odds of metastatic LNs(LODDs)and the 8th edition of the Union for International Cancer Control/American Joint Committee on Cancer(UICC/AJCC)N classification.Results:The optimal cut-off values for Group 1 and 2 were 13 and 9,respectively.The 5-year overall survival(OS)was higher for patients in retrieved Group 1 LNs>13(vs.Group 1 LNs≤13,63.2%vs.57.9%,P=0.005)and retrieved Group 2 LNs>9(vs.Group 2 LNs≤9,72.5%vs.60.7%,P=0.009).Patients staged as pN0-3b were sub classified using this Group 1 and 2 nodal analogy.The OS of pN0-N2 patients in retrieved Group 1 LNs>13 or Group 2 LNs>9 were superior to those in retrieved Group 1 LNs≤13 and Group 2 LNs≤9(All P<0.05);except for pN3 patients.The rN clas-sification was formulated and demonstrated better 5-year OS prognostication performance as compared to the LNR,LODDs,and the 8th UICC/AJCC N staging system.Conclusions:The retrieval of>13 and>9 LNs for Group 1 and Group 2,respectively,could represent an alternative lymph node retrieval approach in radical gastrectomy for more precise survival prognostication and minimizing staging migration,especially if>16 LNs is found to be difficult. | Siwei Pan Pengliang Wang Yanan Xing Kai Li Zhenning Wang Huimian Xu Zhi Zhu | 2019 | Cancer Communications2019，39，1： | 1 |
| 15 | KINETICS OF PREPARATION OF ULTRAFINE TiN POWDER BY HIGH FREQUENCY PLASMA显示文摘The reaction kinetie model for the synthesis of ultrafine TiN powder from vapour phase sys-tem TiCl4-NH3-H2-N2 in the high frequency plasma reactor was proposed as follows:1/V((dnTiCl4)/dt)=0.852e-63.2·RTPTiCl4The majority of factors influenced upon the reaction rate were analysed,so as to providethe theoretical basis for improving quality of TiN powder and for optimizing the design ofplasma reactor. | ZHU Lianxi ZOU Siwei Chengdu University of Science and Technology,Chengdu,China ZHU Lianxi Dept.fo Environmental Science and Engineering,Chengdu University of Science and Technology,Chengdu 610065,China | 1990 | Acta Metallurgica Sinica(English Letters)1990，3，10： | 1 |
| 16 | Achieving white-light emission in a single-component polymer with halogen-assisted interaction显示文摘White-light emitting(WLE) polymers have attracted continuous attention for their promising applications in solid-state lighting,flexible display and related fields. However, achieving dual-emission and pure white-light emission in a single-component polymer is still challenging. In this study, a brominated single-component polymer Br OD-TFB was designed and synthesized,which shows dual-emission and white light emission properties in solution and room-temperature phosphorescence(RTP) in thin films. The dual-emission properties can be tuned by concentration, solvent polarity, and excitation energy. Spectral analyses and theoretical calculations reveal that the origin of the high-energy emission band(HEB) is intramolecular charge transfer(ICT)along the polymer chain, whilst the low-energy emission band(LEB) originates from the excited-state related to the intra-chain and inter-chain C–Br···π interactions as demonstrated by the single-crystal structure of the model compound. Appropriate control of the formation and the destruction of the halogen-assisted interactions can initiate white-light emission in the singlecomponent polymer. More interestingly, by dispersing Br OD-TFB(0.1 wt%) in a non-emissive, colorless and transparent polymer, the characteristics of this white-light emission can be fully demonstrated while exhibiting unexpected RTP properties,with photoluminescence quantum efficiency(Φ_(PL)) of up to 23% and CIE coordinates of(0.32, 0.32). | Zhuxin Zhou Zhu Mao Zhan Yang Tingting Yang Longji Zhu Yubo Long Zhenguo Chi Siwei Liu Matthew P.Aldred Xudong Chen Jiarui Xu Yi Zhang | 2021 | Science China Chemistry2021，64，3： | 1 |
| 17 | Modeling and characterizing the typical under-ice acoustic channel for the Arctic显示文摘The properties of arctic underwater acoustic channel were researched using BurkeTwersky(BT) model and ray theory.In the BT model,sea ice ridge is assumed as a set of semi-elliptical cylinder randomly distributed on a stress-free surface.By approximation of low frequency and high frequency of the BT model,the reflection coefficients were calculated at different frequencies.On the basis of ray theory,the acoustic field was calculated and the properties of underwater acoustic channel was analyzed.Compared to underwater acoustic channel properties when the boundary is air,the results show that under the ice surface some sound rays disappear during transmission,because the ice surface reflection coefficients is less than absolute soft surface.Especially,the higher the frequency,the higher the under-ice transmission loss.As a result,under-ice condition is unfavourable for acoustic signals to travel far.Besides,as to the channel structure,the reflection coefficient of ice layer is smaller,the channel multi-path structure is not significant compared to water-air interface without ice.The research results are valuable for our understanding of hydroacoustic channel and the prediction of the sonar system working in the polar region. | ZHU Guangping YIN Jingwei ZHOU Huanling CHEN Wenjian HU Siwei GUO Longxiang | 2019 | Chinese Journal of Acoustics2019，38，2： | 1 |
| 18 | Investigation of interactive effects of acid fog and ozone on spruce seedlings显示文摘Ozone and acidic fog are considered as two important factors to cause forest decline. In this study ,laboratory experiment was carried out to simulate the effects of rnountainous air pollution on spruce seedlingsat high altitude with high content of ozone | Zou Siwei Zhu Lianxi(Department of Environmental Science and Engineering, Chengdu University of Scienceand Technology , Chengdu 610065 , China)B. Prinz(Department 4 Landesanstdlt fur Immissionsschutz, NRW. 45133 Essen. Germany) | 1995 | Journal of Environmental Sciences1995，7，2： | 1 |
| 19 | Image edge-enhancement in optical microscopy with a phase mismatched spiral phase plate 显示文摘 | WEI Shibiao BU Jing ZHU Siwei | 2011 | Chin Opt Lett2011，9，03： | 1 |
| 20 | Cell-derived extracellular matrix-coated silk fibroin scaffold for cardiogenesis of brown adipose stem cells through modulation of TGF-β pathway显示文摘The cell-derived extracellular matrix(ECM)-modified scaffolds have advantages of mimic tissue specificity and been thought to better mimic the native cellular microenvironment in vitro.ECM derived from cardiac fibroblasts(CFs)are considered as key elements that provide a natural cell growth microenvironment and change the fate of cardiomyocytes(CMs).Here,a new hybrid scaffold is designed based on silk fibroin(SF)scaffold and CFs-derived ECM.CFs were seeded on the SF scaffold for 10days culturing and decellularized to produce CFs-derived ECM-coated SF scaffold.The results showed that the cell-derived ECM-modified silk fibroin scaffold material contained collagen,laminin,fibronectin and other ECM components with myocardial-like properties.Further to explore its effects on brown adipose stem cells(BASCs)differentiation into CMs.We found that the CFderived ECM-coated scaffold also increased the expression of CM-specific proteins(e.g.cardiac troponin T and α-actinin)of BASCs.Notably,the b1-integrin-dependent transforming growth factor-β1 signaling pathway was also involved in the regulation of CF-derived ECM by promoting the differentiation of BASCs into CMs.Overall,these findings provide insights into the bionic manufacturing of engineered cardiac tissues(ECTs)and establish a theoretical basis for the construction of ECTs. | Wei Liu Yanfeng Sun Xiaohui Dong Qi Yin Huimin Zhu Siwei Li Jin Zhou Changyong Wang | 2020 | Regenerative Biomaterials2020，7，4： | 1 |
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