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| 1 | Cotton GhPOX1 encoding plant class Ⅲ peroxidase may be responsible for the high level of reactive oxygen species production that is related to cotton fiber elongation显示文摘The accumulation of reactive oxygen species(ROS) is involved in plant cell development.In plant,class Ⅲ peroxidases are heme-containing enzymes encoded by a large multi-gene family participated in the release or consumption of ROS.The specific function of each member of the family is still elusive.Here,we showed that ROS was significantly generated during cotton fiber initiation and elongation,whereas,application of NADPH oxidase inhibitor diphenyleneiodonium(DPI) and peroxidase inhibitor salicylhydroxamic acid(SHAM) to the wild-type cotton ovule culture significantly suppressed fiber growth,respectively.Their inhibitory effects were caused by the reduction of superoxide radical(O2-).Ten GhPOX genes(cDNAs) encoding cotton class Ⅲ peroxidases were isolated,among them eight GhPOX genes were reported for the first time.Microarray analyses indicated that GhPOX1 was the mostly predomi-nantly expressed in fast-elongating cotton fiber cells.Real-time quantitative PCR analysis revealed the transcript level of GhPOX1 was over 400-fold higher in growing fiber cells than in ovules,flowers,roots,stems and leaves.To reveal the role of GhPOX1 in plant devel-opment,its Arabidopsis orthologue atpox13 mutant was demonstrated to be defective in branch root development.Taken together,the data suggest that GhPOX1 plays an important role during fiber cell elongation possibly by mediating production of reactive oxygen spe-cies. | Wenqian Mei Yongmei Qin Wenqiang Song Jun Li Yuxian Zhu | 2009 | Journal of Genetics and Genomics2009，36，3： | 19 |
| 2 | miR-503-3p promotes epithelialemesenchymal transition in breast cancer by directly targeting SMAD2 and E-cadherin显示文摘Although progress in clinical and basic research has significantly increased our understanding of breast cancer, little is known about the molecular mechanism underlying breast cancer metastasis. Identification of effective therapeutic targets to prevent breast cancer metastasis is urgently needed. The function of mi R-503-3p has been investigated in other cancers, but its role in breast cancer remains undefined.Here, we found that mi R-503-3p was overexpressed in breast cancer tissue and plasma compared with adjacent normal breast tissue and with plasma from healthy individuals. Moreover, we identified mi R-503-3p to be an oncogene of breast cancer cell proliferation, migration and invasion. Upregulation of mi R-503-3p in breast cancer cells inhibited expression of epithelialemesenchymal transition(EMT)-related protein SMAD2 and the epithelial marker protein E-cadherin by directly binding to their m RNA30 untranslated region, whereas increased expression of mesenchymal marker proteins, including vimentin and N-cadherin. Taken together, our findings support a critical role for mi R-503-3p in induction of breast cancer EMT and suggest that plasma mi R-503-3p may be a useful diagnostic biomarker for breast cancer. | Zitong Zhao Xinyi Fan Lanfang Jiang Zhongqiu Xu Liyan Xue Qimin Zhan Yongmei Song | 2017 | Journal of Genetics and Genomics2017，44，2： | 15 |
| 3 | Pediatric reference intervals in China(PRINCE):design and rationale for a large,multicenter collaborative cross-sectional study显示文摘There is a lack of accurate pediatric reference intervals(RIs) in China, with most commonly used RIs established without consideration of the effect of age and gender. The Pediatric Reference Intervals in China(PRINCE) project aims to establish and verify pediatric RIs for 31 common laboratory measurands.The project will be a large, multicenter cross-sectional study:14,490 healthy children and adolescents aged up to 19 years will be surveyed by 10 children's hospitals and one pediatric department of a university hospital. To evaluate the feasibility and efficiency of the study methods, 602 children were surveyed in the pilot phase of the PRINCE study in April 2017: it found that some measurands were distinctly age dependent and that there were differences between values for males and females. The results of the pilot study affirmed the necessity of the PRINCE project for Chinese pediatrics. The pilot also indicated potential difficulties in the full survey, e.g., difficulties in recruiting children aged under 3 years and insufficient collection of blood samples from infants. The operation of the PRINCE project has been modified based on the findings in the pilot study toward improving the validity of the PRINCE project and promoting its openness and transparency. | Xin Ni Wenqi Song Xiaoxia Peng Ying Shen Yaguang Peng Qiliang Li Yan Wang Lixin Hu Yanying Cai Hong Shang Min Zhao Hong Jiang Yaoguo Huang Runqing Mu Wenxiang Chen Mingting Peng Chuanbao Zhang Jie Zeng Chenbin Li Hongling Yang Yongmei Jiang Jin Xu Guixia Li Hongbing Chen Yun Xiang Sancheng Cao Zhenxin Guo Dapeng Chen | 2018 | Science Bulletin2018，63，24： | 12 |
| 4 | Enhanced Expression of miR-425 Promotes Esophageal Squamous Cell Carcinoma Tumorigenesis by Targeting SMAD2显示文摘Esophageal squamous cell carcinoma(ESCC) is one of the most common and deadly cancers in the world. Currently, clinical therapy of ESCC remains limited and the five-year survival rate is poor. The function of miR-425 has been reported in multiple human cancers.However, the tumorigenic role and clinical significance of miR-425 in ESCC remains unclear. We found that enhanced expression of miR-425 in ESCC cell lines not only promoted cell proliferation and colony formation, but also increased cellular metastasis. Furthermore, we revealed the mechanism that miR-425 inhibited the expression of SMAD2 by targeting the second binding site in the 30-untranslated region(30-UTR) in ESCC. This mode of action influenced not only SMAD2 mRNA expression but also protein expression. In addition, we detected the expression of miR-425 in ESCC tissues and plasma. Moreover, we analyzed the relationship between miR-425 expression and SMAD2 m RNA expression. We found that miR-425 was overexpressed in ESCC tissues and the plasma relative to adjacent normal tissues and plasma of healthy individuals. Furthermore, there was a negative correlation between miR-425 expression and SMAD2. Taken together, our results show that miR-425 functions as an oncogene by targeting the 30-UTR of SMAD2 and indicate the potential utility of plasma miR-425 as a novel biomarker for ESCC diagnosis. | Lingyan Liu Zitong Zhao Wei Zhou Xinyi Fan Qimin Zhan Yongmei Song | 2015 | Journal of Genetics and Genomics2015，42，11： | 10 |
| 5 | TRAP1 Shows Clinical Significance and Promotes Cellular Migration and Invasion through STAT3/MMP2 Pathway in Human Esophageal Squamous Cell Cancer显示文摘Tumor necrosis factor receptor-associated protein 1(TRAP1), an important member of mitochondrial heat shock protein 90 family, is involved in multiple biological processes in several types of tumors. However, its pathological role in esophageal squamous cell cancer(ESCC) remains unknown. Herein, we demonstrated the clinical value of TRAP1, and its role in apoptosis and motility in ESCC. The clinical potential of TRAP1 was investigated through immunohistochemical analysis in 328 ESCC samples, which revealed that strong TRAP1 expression was associated with increased risk of lymph node metastasis, while high TRAP1 expression correlated with poor prognosis. Expression of TRAP1 was found to be an independent prognostic factor for patients with ESCC. Additionally, the upregulation of TRAP1 antagonized cisplatin-induced apoptosis while its downregulation sensitized cells to cisplatin-induced apoptosis. As revealed by the transwell assay, TRAP1 overexpression promoted cellular migration and invasion as compared to the control groups. In contrast,silencing of endogenous TRAP1 expression attenuated the ability of migration and invasion. Finally, the molecular mechanism investigated in the present study demonstrated that TRAP1-mediated migration and invasion occurred through STAT3/MMP2 signaling pathway. In conclusion, TRAP1 may be considered as a molecular predictive marker for prognosis and a novel molecular candidate for therapeutic target in ESCC. | Yunwei Ou Lingyan Liu Liyan Xue Wei Zhou Zitong Zhao Bainan Xu Yongmei Song Qimin Zhan | 2014 | Journal of Genetics and Genomics2014，41，10： | 8 |
| 6 | Migfilin promotes migration and invasion in glioma by driving EGFR and MMP-2 signalings:A positive feedback loop regulation显示文摘Glioma is the most common type of primary brain tumors in the central nervous system(CNS). Migfilin occurs in human glioma and enhances cellular motility via the epidermal growth factor receptor(EGFR)pathway. However, the underlying molecular mechanism is not fully understood. In this study, we found that Migfilin promoted matrix metalloproteinase-2(MMP-2) activity, and restrained the expression of tissue inhibitor of metalloproteinase 2(TIMP2), which is an MMP-2 inhibitor. Functional and structural studies showed that the LIM1 domain of Migfilin was required for Migfilin-mediated TIMP2 expression inhibition and MMP-2 activity, and was also necessary in promoting cell motility. Furthermore, Migfilininduced EGFR phosphorylation was greatly reduced by MMP-2 inhibitor(GM6001) or si RNA, while Migfilin-induced MMP-2 activation was also blocked by the EGFR inhibitor(AG1478) or si RNA. MMP-2 and EGFR inhibitors and their si RNAs can block Migfilin-induced migration and invasion, respectively.These results demonstrated that EGFR and MMP-2 signalings may form a positive feedback loop to enhance Migfilin-induced migration and invasion. Finally, we detected that the expression of Migfilin,EGFR phosphorylation(Tyr1173) and MMP-2 activity had a positive correlation in the clinical glioma sample. Taken together, these results suggest that Migfilin is a critical regulator in cellular motility by driving the EGFR-MMP-2 feedback loop, and may be considered as a potential therapeutic target in glioma. | Yunwei Ou Qingnan Wu Chuanyue Wu Xuefeng Liu Yongmei Song Qimin Zhan | 2017 | Journal of Genetics and Genomics2017，44，12： | 7 |
| 7 | Downregulation of miR-503 Promotes ESCC Cell Proliferation,Migration,and Invasion by Targeting Cyclin D1显示文摘Esophageal squamous cell carcinoma(ESCC) is one of the most aggressive cancers in China,but the underlying molecular mechanism of ESCC is still unclear.Involvement of microRNAs has been demonstrated in cancer initiation and progression.Despite the reported function of miR-503 in several human cancers,its detailed anti-oncogenic role and clinical significance in ESCC remain undefined.In this study,we examined miR-503 expression by q PCR and found the downregulation of miR-503 expression in ESCC tissue relative to adjacent normal tissues.Further investigation in the effect of miR-503 on ESCC cell proliferation,migration,and invasion showed that enhanced expression of miR-503 inhibited ESCC aggressive phenotype and overexpression of CCND1 reversed the effect of miR-503-mediated ESCC cell aggressive phenotype.Our study further identified CCND1 as the target gene of miR-503.Thus,miR-503 functions as a tumor suppressor and has an important role in ESCC by targeting CCND1. | Lanfang Jiang Zitong Zhao Leilei Zheng Liyan Xue Qimin Zhan Yongmei Song | 2017 | Genomics, Proteomics & Bioinformatics2017，15，3： | 5 |
| 8 | Novel approach to control adsorbent aggregation: iron fixed bentonite-fly ash for Lead (Pb) and Cadmium (Cd) removal from aqueous media显示文摘 | Teza Mwamulima Xiaolin Zhang Yongmei Wang Shaoxian Song Changsheng Peng | 2018 | Frontiers of Environmental Science & Engineering2018，12，2： | 5 |
| 9 | Advanced multiple response surface method of sensitivity analysis for turbine blisk reliability with multi-physics coupling显示文摘To reasonably implement the reliability analysis and describe the significance of influencing parameters for the multi-failure modes of turbine blisk, advanced multiple response surface method(AMRSM) was proposed for multi-failure mode sensitivity analysis for reliability. The mathematical model of AMRSM was established and the basic principle of multi-failure mode sensitivity analysis for reliability with AMRSM was given. The important parameters of turbine blisk failures are obtained by the multi-failure mode sensitivity analysis of turbine blisk. Through the reliability sensitivity analyses of multiple failure modes(deformation, stress and strain) with the proposed method considering fluid–thermal–solid interaction, it is shown that the comprehensive reliability of turbine blisk is 0.9931 when the allowable deformation, stress and strain are3.7*10^(-3)m, 1.0023*10~9 Pa and 1.05*10^(-2)m/m, respectively; the main impact factors of turbine blisk failure are gas velocity, gas temperature and rotational speed. As demonstrated in the comparison of methods(Monte Carlo(MC) method, traditional response surface method(RSM), multiple response surface method(MRSM) and AMRSM), the proposed AMRSM improves computational efficiency with acceptable computational accuracy. The efforts of this study provide the AMRSM with high precision and efficiency for multi-failure mode reliability analysis, and offer a useful insight for the reliability optimization design of multi-failure mode structure. | Zhang Chunyi Song Lukai Fei Chengwei Lu Cheng Xie Yongmei | 2016 | Chinese Journal of Aeronautics2016，29，4： | 4 |
| 10 | RASSF6-TRIM16 axis promotes cell proliferation,migration and invasion in esophageal squamous cell carcinoma显示文摘Ras-association(RA) domain family number 6(RASSF6) is a member of the Ras-association domain protein family.It is epigenetically inactive and negatively regulates the malignant progression of some tumors.However,its precise role in esophageal squamous cell carcinoma(ESCC) has not been reported.In this study,we performed immunohistochemistry(IHC) assay.The results show that RASSF6 is upregulated in ESCC and that the elevated expression level of RASSF6 is associated with lymph node metastasis and poor survival of ESCC patients.Consistent with the clinical obse rvations,the upregulation of RASSF6 greatly promotes ESCC cell proliferation,migration and invasion as well as the cell cycle transition to Gl/S phase in vitro.According to models in vivo,the downregulation of RASSF6 considerably inhibits ESCC tumor growth and lung metastasis.Mechanistically,RASSF6 negatively regulates the tumor suppressor tripartite-motif-containing protein 16(TRIM16) by promoting its ubiquitination-dependent degradation and eventually activates pathways associated with the cell cycle and epithelialmesenchymal transition(EMT).Together,these results indicate that the RASSF6-TRIM16 axis is a key effector in ESCC progression and that RASSF6 serves as a potential target for the treatment of ESCC. | Leilei Zheng Zitong Zhao Lulu Rong Liyan Xue Yongmei Song | 2019 | Journal of Genetics and Genomics2019，46，10： | 3 |
| 11 | Flotillin1 promotes EMT of human small cell lung cancer via TGF-β signaling pathway显示文摘Objective:Small cell lung carcinoma(SCLC)is considered one of the most aggressive types of lung cancer due to its rapid growth and early metastasis.No tumor markers or therapeutic targets have been demonstrated to be specific or effective in SCLC to date.This study aims to evaluate the potential of Flotillin1(Flot1)as a target of SCLC treatment.Methods:Flot1 expression level in the tissue of SCLC and other tissue of lung disease was detected using immunohistochemical staining.Transwell and Matrigel assays were employed to examine migration and invasion of cancer cells.Flow cytometry and xCELLigence system were used to evaluate cell apoptosis and cell viability,respectively.Expression levels of Flot1,epithelialmesenchymal transition(EMT)marker E-cadherin,vimentin,cyclinD1,TGF-β-Smad2/3,and p-AKT were examined using Western blot.Furthermore,xenograft tumor in nude mice was used to evaluate the growth and metastasis of NCI-H446 cells in vivo.Results:Our results demonstrated that Flot1 is highly expressed in SCLC samples and that its expression correlates strongly with clinical stage,distant metastasis,and poor survival.The knockdown of Flot1 decreased the growth,migration,and invasiveness of SCLC cells and reversed EMT phenotype in vitro and in vivo,while enhanced Flot1 expression exhibited the opposite behavior.Gene expression profile analysis demonstrated that Flot1-regulated genes frequently mapped to the AKT and TGF-β-Smad2/3pathways.Our results further revealed that Flot1 affected the progression of SCLC via regulation of EMT progression.Conclusions:These findings indicated an oncogenic role of Flot1 via promoting EMT in SCLC and suggested its potential as a tumor marker and prognostic indicator. | Lianmei Zhao Jie Li Yueping Liu Wei Zhou Yanan Shan Xinyi Fan Xinliang Zhou Baoen Shan Yongmei Song Qimin Zhan | 2018 | Cancer Biology & Medicine2018，15，4： | 3 |
| 12 | Antibacterial Effects of Chinese Herbal Medicines against Streptococcus iniae in Oreochromis niloticus显示文摘In this study,the antibacterial effects of Chinese herbal medicines against Streptococcus iniae in Oreochromis niloticus were investigated by drug sensitivity test. The results indicated that Streptococcus iniae was extremely sensitive to Galla Chinensis,Fructus Hippophae and Fructus Mume,highly sensitive to Sappan Lignum,Pericarpium Granati,Folium Eucalypti and Radix Scutellariae,moderately sensitive to Rhizoma Coptidis,Radix et Rhizoma Rhei,Flos Caryophyllata,Cortex phellodendri and Rabdosia serra( Maxim.) Hara,and insensitive to Herba Portulacae,Herba Houttuyniae,Polygonum hydropiper L.,Herba Menthae,Radix Glycyrrhizae,Pericarpium Citri Reticulatae,Herba Andrographitis and Rhizoma Acori Tatarinowii. Chinese medicinal herbs which Streptococcus iniae was extremely sensitive or moderately sensitive to were selected and prepared into three Chinese herbal medicine formulae for medicated bath of Oreochromis niloticus infected artificially with Streptococcus iniae. The results showed that medicated bath elicited therapeutic effects on infected Oreochromis niloticus. Formula II exhibited the best therapeutic effect with an effective percentage of 90%. | Guisheng GAO Yanying ZHANG Yongmei SU Qiumei SHI Guangping GAO Yating BAI Hongsheng HAN Jianxin ZHU Qingchun SONG Guanglian REN | 2016 | Agricultural Biotechnology2016，5，1： | 2 |
| 13 | iRGD decorated liposomes:A novel actively penetrating topical ocular drug delivery strategy显示文摘Ocular drug delivery remains a significant challenge that is limited by poor corneal retention and permeation,resulting in low ocular bioavailability(<5%).Worse still,the most convenient and safe route of ocular drug administration,topical administration results in a drug bioavailability of less than 1%.iRGD modified drug delivery strategies have been developed for cancer therapy,however active targeting iRGD platforms for ocular drug delivery have yet to be explored.Herein,an iRGD modified liposomes was developed for ocular drug delivery via topical administration.The results indicated that iRGD modified liposomes could prolong the corneal retention time and enhance corneal permeability in an iRGD receptor mediated manner.These findings provided a novel strategy for topical ocular drug delivery for the treatment of posterior ocular diseases. | Hai Huang Xiaorong Yang Huili Li Hansi Lu James Oswald Yongmei Liu Jun Zeng Chaohui Jin Xingchen Peng Jiyan Liu Xiangrong Song | 2020 | Nano Research2020，13，11： | 2 |
| 14 | Deregulated expression and subcellular localization of CPSF6,a circRNA-binding protein,promote malignant development of esophageal squamous cell carcinoma显示文摘Objective:Cleavage and polyadenylation specific factor 6(CPSF6)has been documented as an oncoprotein in different types of cancer.However,functions of CPSF6 have not been investigated yet in esophageal squamous cell carcinoma(ESCC).Here,we aimed to investigate the potential clinical values and biological functions of CPSF6 in ESCC.Methods:For determining the expression level of CPSF6 in ESCC patients,we analyzed published data,performed quantitative real-time polymerase chain reaction(RT-qPCR)and immunohistochemistry assays.Kaplan-Meier curves and log-rank tests were used for survival analyses.GO and KEGG analyses were done for CPSF6-related genes.Cell proliferation,colony formation and xenograft assays were conducted to verify the effects of CPSF6 on ESCC.In addition,cell cycle and apoptosis assays were also performed to manifest the functions of CPSF6 and circCPSF6.RNA pulldown and radioimmunoprecipitation(RIP)assays were used for confirming the interaction between circCPSF6(hsa_circ_0000417)and CPSF6 protein.The regulatory relationship between CPSF6 protein and circCPSF6 was determined by RT-qPCR.Results:We found that CPSF6 was upregulated in ESCC tissues and overexpression of cytoplasmic CPSF6 was associated with poor prognosis.GO and KEGG analyses suggested that CPSF6 could mainly affect cell division in ESCC.Further experiments manifested that CPSF6 promoted cell proliferation and colony formation in vitro.Xenograft assay showed that knockdown of CPSF6 significantly decreased tumor growth rate in vivo.Subsequently,we verified that depletion of CPSF6 led to cell cycle arrest and apoptosis.Finally,we validated that CPSF6,as a circRNA-binding protein,interacted with and regulated its circular isoform circCPSF6(hsa_circ_0000417),of which depletion also resulted in cell cycle arrest and cell apoptosis in ESCC.Conclusions:These findings gave us insight that overexpression of cytoplasmic CPSF6 protein is associated with poor prognosis in ESCC and CPSF6 may function as an oncoprotein,at least in part,through regulating circCPSF6 expression. | Shichao Guo Guangchao Wang Zitong Zhao Dan Li Yongmei Song Qimin Zhan | 2022 | Chinese Journal of Cancer Research2022，34，1： | 1 |
| 15 | Analysis of Paralytic Shellfish Toxins in Aphanizomenon DC - 1 from Lake Dianchi, China 显示文摘 | Liu Yongmei Chen Wei Li Dunhai Shen Yinwu Liu Yongding Song Lirong | 2006 | Environ Toxicol2006，21，： | 1 |
| 16 | Reinvestigation on the ozonation ofN-nitrosodimethylamine: Influencing factors and degradationmechanism显示文摘 | Juan Lv Yongmei Li Yun Song | 2013 | Water Research2013，5，35： | 1 |
| 17 | Drogue detection for vision-based autonomous aerial refueling via low rank and sparse decomposition with multiple features 显示文摘 | GAO Shibo CHENG Yongmei SONG Chunhua | 2013 | Infrared Physics and Technology(S1350-4495)2013，60，9： | 1 |
| 18 | PTBP1 promotes IRES-mediated translation of cyclin B1 in cancer显示文摘Cyclin B1 is an essential cyclin-dependent protein that involves in the G2/M transition.Multiple studies report that cyclin B1 is upregulated in cancers and promotes cancer progression.However,the mechanism of cyclin B1 upregulation remains unclear.Here we report that the 5′UTR of cyclin B1 mRNA contains an internal ribosome entry site(IRES)by using a bicistronic fluorescent reporter.We show that IRES can initiate the translation of cyclin B1,and the IRES-mediated translation is further activated under cell stress.Interacting trans-acting factors(ITAFs)are required by most IRES to initiate the translation.We find that PTBP1 promotes the IRES-mediated translation of cyclin B1 by binding to the 5′UTR of cyclin B1.On top of that,PTBP1 promotes the malignancy of ESCC cells.Our data suggest that the IRES-mediated translation of cyclin B1 plays an essential role in the cyclin B1 upregulation in cancers. | Xinyi Fan Zitong Zhao Liying Ma Xuanlin Huang Qimin Zhan Yongmei Song | 2022 | Acta Biochimica et Biophysica Sinica2022，54，5： | 1 |
| 19 | Indoor positioning simulation based on Landmarc system 显示文摘 | Li Xingpeng Hu YongMei Song Jibo | 2008 | The Computer Engineering and Application2008，，27： | 1 |
| 20 | Quiescence and attenuated DNA damage response promote survival of esophageal cancer stem cells显示文摘 | Yulin Chen Dan Li Dapeng Wang Xuefeng Liu Ning Yin Yongmei Song Shih Hsin Lu Zhenyu Ju Qimin Zhan | 2012 | J Cell Biochem2012，，12： | 1 |
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