|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Natural polymer-derived photocurable bioadhesive hydrogels for sutureless keratoplasty显示文摘Keratoplasty is the gold standard treatment for visual impairment caused by corneal damage.The use of suturing as the bonding method is the source of many complications following keratoplasty.Currently available corneal adhesives do not have both adequate adhesive strength and acceptable biocompatibility.Herein,we developed a photocurable bioadhesive hydrogel which was composed of gelatin methacryloyl and oxidized dextran for sutureless keratoplasty.The bioadhesive hydrogel exhibited high light transmittance,resistance to enzymatic degradation and excellent biocompatibility.It also had higher adhesive strength than commercial adhesives(fibrin glue).In a rabbit model of lamellar keratoplasty,donor corneal grafts could be closely bonded to the recipient corneal bed and remained attached for 56 days by using of this in situ photopolymerized bioadhesive hydrogel.The operated cornea maintained transparent and noninflamed.Sutureless keratoplasty using bioadhesive hydrogel allowed rapid graft re-epithelialization,typically within 7 days.In vivo confocal microscopic and histological evaluation of the operated cornea did not show any apparent abnormalities in terms of corneal cells and ultrastructure.Thus,this bioadhesive hydrogel is exhibited to be an appealing alternative to sutures for keratoplasty and other corneal surgeries. | Xuan Zhao Saiqun Li Xinyue Du Weihua Li Qian Wang Dalian He Jin Yuan | 2022 | Bioactive Materials2022，7，2： | 4 |
| 2 | Cloning and identification of a novel RNF6 transcrip-tional splice variant Spg2 in human development显示文摘RING finger proteins are zinc finger proteins containing the RING motifs. They act mainly as E3 ubiq-uitin ligases, bind the ubiquitin E2 conjugating enzyme and promote degradation of targeted proteins, Many novel genes have been isolated and differentially expressed in human adult and embryo testis by a testis cDNA-array differential display technique. A novel RING finger cDNA is highly expressed in adult testis and at low level in fetal testis. It was named Spg2. It contains a 2055 nucleotide ORF, en-codes a 685-amino-acid RNF6 protein, and has a RING finger in its C terminal. NCBI Blast shows that the gene is located on chromosome 13 and contains five exons. A multiple tissue expression profile also indicates that it is highly expressed in human testis, so we speculate that it may be associated with human spermatogenesis by virtue of the action of its RING domain. | ZHU Li TONG GuoQin CHEN Jie WANG Yun WANG SaiQun ZHAO MuZhi LI JianMin MA JianHua | 2008 | Science China(Life Sciences)2008，51，4： | 3 |
| 3 | Dual-crosslinked regenerative hydrogel for sutureless long-term repair of corneal defect显示文摘Corneal transplantation is the most effective clinical treatment for corneal defects,but it requires precise size of donor corneas,surgical sutures,and overcoming other technical challenges.Postoperative patients may suffer graft rejection and complications caused by sutures.Ophthalmic glues that can long-term integrate with the corneal tissue and effectively repair the focal corneal damage are highly desirable.Herein,a hybrid hydrogel consisting of porcine decellularized corneal stroma matrix(pDCSM)and methacrylated hyaluronic acid(HAMA)was developed through a non-competitive dual-crosslinking process.It can be directly filled into corneal defects with various shapes.More importantly,through formation of interpenetrating network and stable amide bonds between the hydrogel and adjacent tissue,the hydrogel manifested excellent adhesion properties to achieve suture-free repair.Meanwhile,the hybrid hydrogel not only preserved bioactive components from pDCSM,but also exhibited cornea-matching transparency,low swelling ratio,slow degradation,and enhanced mechanical properties,which was capable of withstanding superhigh intraocular pressure.The combinatorial hydrogel greatly improved the poor cell adhesion performance of HAMA,supported the viability,proliferation of corneal cells,and preservation of keratocyte phenotype.In a rabbit corneal stromal defect model,the experimental eyes treated with the hybrid hydrogel remained transparent and adhered intimately to the stroma bed with long-term retention,accelerated corneal re-epithelialization and wound healing.Giving the advantages of high bioactivity,low-cost,and good practicality,the dual-crosslinked hybrid hydrogel served effectively for long-term suture-free treatment and tissue regeneration after corneal defect. | Xuanren Shen Saiqun Li Xuan Zhao Jiandong Han Jiaxin Chen Zilong Rao Kexin Zhang Daping Quan Jin Yuan Ying Bai | 2023 | Bioactive Materials2023，，2： | 1 |
| 4 | A novel role for DYX1C1, a chaperone protein for both Hsp70 and Hsp90, in breast cancer显示文摘 | Yuxin Chen Muzi Zhao Saiqun Wang Jie Chen Yun Wang Qinhong Cao Wenbin Zhou Jin Liu Zhiyang Xu Guoqing Tong Jianmin Li | 2009 | Journal of Cancer Research and Clinical Oncology2009，，9： | 1 |
| 5 | A Feasible and Novel Multi-Generation System Taking Advantage of Two-Stage Compressor for Luxury Hotels显示文摘In order to satisfy heating,cooling and domestic hot water demand and energy conversion in luxury hotels,a feasible and novel multi-generation system taking advantage of the two-stage compressor was proposed and analyzed.The feature of this novel system is that the compressing power can be reduced and domestic water can be generated at the same time by adopting two-stage compressors instead of conventional one-stage compressor with a high compressing ratio.The energy-saving mechanism and two operating modes were introduced in detail.In a further way,the thermodynamic performance was calculated by Aspen Plus so as to make a better understanding of this novel system.And the calculation results showed that,in summer and transition seasons operating mode,the system can obtain COP(Coefficient of Performance)7.3,and 60℃ domestic hot water,when evaporating temperature is 4℃;while,in winter season operating mode,the system can obtain COP 3.1,and 58℃ domestic hot water,when evaporating temperature is-24℃.Compared with the conventional independent systems,this system can achieve higher energy-efficient,higher waste heat recovery,reduce energy consumption,and meet multiple energy demands simultaneously. | LI Hongqiang ZHOU Saiqun HE Changjie DENG Lu LI Lu LI Shuisheng ZHANG Guoqiang | 2021 | Journal of Thermal Science2021，30，3： | 1 |
| 6 | Corneal biomechanical changes in allergic conjunctivitis显示文摘Background:To explore corneal biomechanical changes,identify related factors and determine early indicators of keratoconus(KC)development risk in allergic conjunctivitis(AC)patients.Methods:A total of 50 patients,including 20 eyes without AC and 30 eyes with AC were enrolled in this study.All patients underwent a complete ocular examination,including evaluations of clinical manifestations of AC,corneal tomography and densitometry by Pentacam,corneal biomechanics by Corvis ST,and corneal and epithelial thickness mapping by RTvue optical coherence tomography(OCT).Results:The index of surface variance(ISV),index of vertical asymmetry(IVA),keratoconus index(KI),index of height decentration(IHD)and Belin/Ambrosio enhanced ectasia total deviation index(BAD-D)were significantly higher in the AC group than in the non-allergic conjunctivitis(NAC)group(P<0.05).The tomography and biomechanical index(TBl)was also significantly higher in the AC group(P=0.04).The average epithelial thickness in the 2-7 mm annulus was significantly thinner in the AC group than in the NAC group(P<0.05).The average densitometry of the total cornea and the anterior layer were higher in the AC group than in the NAC group(P<0.001).The ISV,IVA,KI,IHD and BAD-D were significantly correlated with the TBI and changes in corneal epithelial thickness in AC patients(P<0.05).The changes in epithelial thickness were closely related to the eye rubbing frequency and allergic sign scores(P<0.05).Conclusions:AC patients should be advised to routinely undergo corneal tomographic and biomechanical measurements,and the TBl could be used as an indicator of KC development risk in AC patients.Trial registration:Corneal Biomechanical Changes of Allergic Conjunctivitis,NCT04299399.Registered March 3,2020-Retrospectively registered. | Qian Wang Yuqing Deng Saiqun Li Xinyue Du Xuan Zhao Tingting Zhang Jin Yuan | 2023 | Eye and Vision2023，9，4： | 0 |
| 7 | The inter-visit variability of retinal blood flow velocity measurements using retinal function imager (RFI)显示文摘Background:To determine the inter-visit variability of retinal blood flow velocities(BFVs)using a retinal function imager(RFI)in healthy young subjects.Methods:Twenty eyes of 20 healthy young subjects were enrolled.RFI imaging was performed to obtain the BFVs in retinal arterioles and venules in a field measuring 7.3×7.3 mm^(2)(setting:35 degrees)centered on the fovea,and repeated measurements were obtained on two separate days.The inter-visit variability of BFVs was assessed by the concordance correlation coefficient(CCC)and coefficient of variance(CV).Results:At the first visit,the mean BFV was 3.6±0.8 mm/s and 3.0±0.7 mm/s in arterioles and venules,respectively,which were not significantly different from those at the second visit(the BFV of arterioles was 3.5±0.8 mm/s,and the BFV of venules was 3.0±0.7 mm/s,P>0.05,respectively).The CCC was 0.72 in the BFVs of arterioles and 0.67 in venules,and the CV was 10.8%in the BFVs of arterioles and 11.0%in venules.Conclusion:The inter-visit variability using the retinal function imager(RFI)with a large field of view appeared to be good and comparable to previously reported intra-visit and inter-eye variability. | Yuqing Deng Meng Li Gengyuan Wang Hong Jiang Jianhua Wang Jing Zhong Saiqun Li Jin Yuan | 2018 | Eye and Vision2018，5，1： | 0 |
| 8 | Corneal biomechanical changes in allergic conjunctivitis显示文摘Background:To explore corneal biomechanical changes,identify related factors and determine early indicators of keratoconus(KC)development risk in allergic conjunctivitis(AC)patients.Methods:A total of 50 patients,including 20 eyes without AC and 30 eyes with AC were enrolled in this study.All patients underwent a complete ocular examination,including evaluations of clinical manifestations of AC,corneal tomography and densitometry by Pentacam,corneal biomechanics by Corvis ST,and corneal and epithelial thickness mapping by RTvue optical coherence tomography(OCT).Results:The index of surface variance(ISV),index of vertical asymmetry(IVA),keratoconus index(KI),index of height decentration(IHD)and Belin/Ambrosio enhanced ectasia total deviation index(BAD-D)were significantly higher in the AC group than in the non-allergic conjunctivitis(NAC)group(P<0.05).The tomography and biomechanical index(TBI)was also significantly higher in the AC group(P=0.04).The average epithelial thickness in the 2-7mm annulus was significantly thinner in the AC group than in the NAC group(P<0.05).The average densitometry of the total cornea and the anterior layer were higher in the AC group than in the NAC group(P<0.001).The ISV,IVA,KI,IHD and BAD-D were significantly correlated with the TBI and changes in corneal epithelial thickness in AC patients(P<0.05).The changes in epithelial thickness were closely related to the eye rubbing frequency and allergic sign scores(P<0.05).Conclusions:AC patients should be advised to routinely undergo corneal tomographic and biomechanical measurements,and the TBI could be used as an indicator of KC development risk in AC patients. | Qian Wang Yuqing Deng Saiqun Li Xinyue Du Xuan Zhao Tingting Zhang Jin Yuan | 2021 | Eye and Vision2021，8，1： | 0 |
| 9 | Metabolomics Analysis of Metabolites in Forsythia suspense Fruit Using UPLC/ESI-Q TRAP-MS/MS显示文摘Forsythiae Fructus,the fruit of Forsythia suspense is a traditional Chinese hebal medicine that has the antiviral and antioxidant effects in China.Modern analytical chemistry studies showed that the extracts of Forsythiae Fructus contain many bioactive components,such as flavonoids,lignans,phenolic acids,and terpenoids,which can be used to anti-inflammatory and treat toxicity,tonsillitis,ulcers,pharyngitis and acute nephritis.In order to study the types and quantities of metabolites in Forsythiae Fructus,we isolated,identified and analysed metabolites between two varieties of Forsythiae Fructus using UPLC/ESI-Q TRAP-MS/MS.The results showed that a total of 407 metabolites were identified in Forsythiae Fructus using UPLC/ESI-Q TRAP-MS/MS,including 21 terpenoids,68 phenolic acids,63 flavonoids,43 amino acids and derivatives,22 alkaloids,55 lipids,24 lignans and coumarins,31 nucleotides and derivatives,29 organic acids,and 51 other metabolites.Among,lignans and coumarins,terpenoids,organic acids,lipids,and phenolic acids were rich in Forsythiae Fructus,which accounted for more than 60%of the total metabolite content.Differential metabolite analysis revealed that 80 metabolites differed significantly between the two types of Forsythiae Fructus.Our results greatly enrich the Forsythiae Fructus phytochemical composition database and provide valuable information for further study of the metabolites of Forsythiae Fructus. | Lingdi Liu Chunxiu Wen Wei Tian Xiaoliang Xie Saiqun Wen Tao Jiang | 2022 | Phyton-International Journal of Experimental Botany2022，91，10： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员