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| 1 | Indirect printing of hierarchical patient-specific scaffolds for meniscus tissue engineering显示文摘The complex meniscus tissue plays a critical role in the knee. The high susceptibility to injury has led to an intense pursuit for better tissue engineering regenerative strategies, where scaffolds play a major role. In this study, indirect printed hierarchical multilayered sca ffolds composed by a silk fibroin (SF) upper layer and an 80/20 (w/w) ratio of SF/ionic-doped β-tricalcium phosphate (TCP) bottom layer were developed. Furthermore, a comparative analysis between two types of sca ffolds pro- duced using di fferent SF concentrations, i.e., 8% (w/v) (Hi8) and 16% (w/v) (Hi16) was performed. In terms of architecture and morphology, the produced sca ffolds presented homogeneous porosity in both layers and no di fferences were observed when comparing both sca ffolds. A decrease in terms of mechanical performance of the sca ffolds was observed when SF concentration decreased from 16 to 8% (w/v). Hi16 revealed a static compressive modulus of 0.66 ± 0.05 MPa and dynamical mechanical properties ranging from 2.17 ± 0.25 to 3.19 ± 0.38 MPa. By its turn, Hi8 presented a compressive modulus of 0.27 ± 0.08 MPa and dynamical mechanical properties ranging from 1.03 ± 0.08 MPa to 1.56 ± 0.13 MPa. In vitro bioactivity studies showed formation of apatite crystals onto the surface of Hi8 and Hi16 bottom layers. Human meniscus cells (hMCs) and human primary osteoblasts were cultured separately onto the top layer (SF8 and SF16) and bottom layer (SF8/TCP and SF16/TCP) of the hierarchical sca ffolds Hi8 and Hi16, respectively. Both cell types showed good adhesion and proliferation as denoted by the live/dead staining, Alamar Blue assay and DNA quanti fication analysis. Subcutaneous implantation in mice revealed weak in flammation and sca ffold’s integrity. The hierarchical indirect printed SF sca ffolds can be promising candidate for meniscus TE sca ffolding applications due their suitable mechanical properties, good biological performance and possibility of being applied in a patient-speci fic approach. | Joao BCosta Joana Silva-Correia Sandra Pina Alain da Silva Morais Sílvia Vieira Hélder Pereira Joao Espregueira-Mendes Rui LReis Joaquim M.Oliveira | 2019 | Bio-Design and Manufacturing2019，2，4： | 2 |
| 2 | Induction of cytochrome c-mediated apoptosis by amyloid β 25- 35 requires functional mitochondria 显示文摘 | Sandra M C Russell H S Catarina R O | 2002 | Brain Research2002，931，2： | 1 |
| 3 | Induction of cytochrome e-mediated apoptosis by amyloid β 25-35 requires functional mitochondria显示文摘 | Sandra M C Russell H S Catarina R O | 2002 | Brain Research2002，931，2： | 1 |
| 4 | Developments in nanoscienee: Polyhedral oligomerie silsesquioxane (POSS)-polymers显示文摘 | Shawn H P Timothy S H Sandra J T | 2004 | Current Opinion in Solid State and Materials Science2004，8，1： | 1 |
| 5 | Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses显示文摘 | Jianhua S William C H Sandra P | 2009 | Nature Structural & Molecular Biology2009，14，： | 1 |
| 6 | Mobility and safety in the multiple myeloma survivor:survivorship care plan of the International Myeloma Foundation Nurse Leadership Board 显示文摘 | Sandra I Rome H Bonnie S | 2011 | Clinical Journal of Oncolo- gy Nursing2011，12，15： | 1 |
| 7 | Effect of LiCl on the stabile length of electrospinning jet by PAN polymer solution显示文摘 | Qin X H Wang S Y Sandra T | 2005 | Material Letters2005，59，： | 1 |
| 8 | Production and characterization of Monoclonal antibodies against sarafloxacin and crossreactivity studies of related fluoroquinolones显示文摘 | Carol K H Sandra A B Larry H S | 1997 | J Agric Food Chem1997，，： | 1 |
| 9 | Developments in nanoscience: polyhedral oligomeric silsesquioxane (POSS)-polymers显示文摘 | Shawn H Phillips Timothy S Haddad Sandra J Tomczak | 2004 | Current Opinion in Solid State & Materials Science2004，，1： | 1 |
| 10 | TAT-mediated protein transduction into mammalian cells 显示文摘 | Michelle B H Sandra S M Dowdy S F | 2001 | METHODS2001，24，： | 1 |
| 11 | Cytogenetic findings in a case of nodular fasciitis of the breast显示文摘 | Sandra H B Janet M S Brenda M S | 1995 | Cancer Genet Cytogenet1995，81，： | 1 |
| 12 | Developments in nanosciece:polyhedral oligomeric silsesquioxane (POSS)-polymers显示文摘 | Shawn H Phillips Timothy S Haddad Sandra J Tomczak | 2004 | Current Opinion in State and Materials Science2004，8，1： | 1 |
| 13 | The effect of formalin on acetylcholinesterase and catalase activities,and on the concentration of oximes,in the earthworm species Eisenia andrei显示文摘 | BRANIMIR K H MIRNA V SANDRA S | | 0，，： | 1 |
| 14 | Microstructuralinvestigations of natural and synthetic graphites and semi-graphites显示文摘 | SANDRA H MANUELA M ISABEL S R | 2013 | International Journal of Coal Geology2013，111，： | 1 |
| 15 | Lipoprotein lipase and apoE polymorphisms: relationship to hypertriglyceridemia during pregnancy显示文摘 | Sandra H McGladdery S H Jiri J | 2001 | J Lipid Res2001，42，11： | 1 |
| 16 | Numerical calculation of pressure fluctuations in the volute of a centrifugal fan 显示文摘 | RAFAEL B T SANDRA V S JUAN PABLO H C | 2006 | Journal of Fluids Engineering2006，3，： | 1 |
| 17 | Chromium is the proximate clastogenic species for lead chromate-induced clastogenicity in human bronchial cells显示文摘 | SANDRA S W AMIE L H MICHAEL E K | 2004 | Mutation Research2004，560，： | 1 |
| 18 | High-yield expression of a viral peptide animal vaccine in transgenic tobacco chloroplasts显示文摘 | Andrea M Sandra H S Henry D | 2004 | Plant Biotechnology Journal2004，2，2： | 1 |
| 19 | Selection of peptides with semiconductor binding opecificity for directed nanocrystal assembly显示文摘 | Sandra R Apley W H English D S | 2000 | Nature2000，405，8： | 1 |
| 20 | Zircon M257-a homogeneous natural reference material for the ion microprobe U-Pb analysis of zircon显示文摘 | Nasdala L Norberg N Schaltegger U Lutz N Wolfgang H Nicholas N James M M Fernando C Wolfgang D Sandra L K Allen K K Andreas K Peter W R Dirk F Jan K Wan Y S Jens G Tobias H Alfred K John W V | 2008 | Geostandards and Geoanalytical Research2008，32，： | 1 |
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