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| 1 | Micro RNA-320 family is downregulated in colorectal adenoma and affects tumor proliferation by targeting CDK6显示文摘AIM: To investigate the microR NA(miR NA) expression during histological progression from colorectal normal mucosa through adenoma to carcinoma within a lesion. METHODS: Using microarray, the sequential changes in miR NA expression profiles were compared in colonic lesions from matched samples; histologically, nonneoplastic mucosa, adenoma, and submucosal invasive carcinoma were microdissected from a tissue sample. Cell proliferation assay was performed to observe the effect of miR NA, and its target genes were predicted using bioinformatics approaches and the expression profile of SW480 transfected with the miR NA mimics. mR NA and protein levels of the target gene in colon cancer cell lines with a mimic control or miR NA mimics were measured using qR TPCR and Western blotting. The expression levels of miR NA and target gene in colorectal tissue samples were also measured.RESULTS: Microarray analysis identified that the miR-320 family, including miR-320 a, miR-320 b, miR-320 c, miR-320 d and miR-320 e, were differentially expressed in adenomaand submucosal invasive carcinoma. The mi R-320 family, which inhibits cell proliferation, is frequently downregulated in colorectal adenoma and submucosal invasive carcinoma tissues. Seven genes including CDK6 were identified to be common in the results of gene expression array and bioinformatics analyses performed to find the target gene of the miR-320 family. We confirmed that mR NA and protein levels of CDK6 were significantly suppressed in colon cancer cell lines with miR-320 family mimics. CDK6 expression was found to increase from non-neoplastic mucosa through adenoma to submucosal invasive carcinoma tissues and showed an inverse correlation with miR-320 family expression.CONCLUSION: MiR-320 family affects colorectal tumor proliferation by targeting CDK6, plays important role in its growth, and is considered to be a biomarker for its early detection. | Toshihiro Tadano Yoichi Kakuta Shin Hamada Yosuke Shimodaira Masatake Kuroha Yoko Kawakami Tomoya Kimura Hisashi Shiga Katsuya Endo Atsushi Masamune Seiichi Takahashi Yoshitaka Kinouchi Tooru Shimosegawa | 2016 | World Journal of Gastrointestinal Oncology2016，8，7： | 9 |
| 2 | Significance of K-ras mutation and CEA level in pancreatic juice in the diagnosis of pancreatic cancer显示文摘 | Noriaki Futakawa Wataru Kimura Seiichi Yamagata Bin Zhao Han Ilsoo Tomomi Inoue Naohiro Sata Yoneei Kawaguchi Yoshiro Kubota Tetsuichiro Muto | 2000 | Journal of Hepato - Biliary - Pancreatic Surgery2000，，1： | 2 |
| 3 | The gene for hepatocyte nuclear factor (HNF)-4α is activated by glucocorticoids and glucagon, and repressed by insulin in rat liver显示文摘 | Seiichi Oyadomari Fumihiko Matsuno Shoaib Chowdhury Tatsuya Kimura Katsuro Iwase Eiichi Araki Motoaki Shichiri Masataka Mori Masaki Takiguchi | 2000 | FEBS Letters2000，，1： | 1 |
| 4 | Significance of K-ras mutation and CEA level in pancreatic juice in the diagnosis of pancreatic cancer显示文摘 | Noriaki Futakawa Wataru Kimura Seiichi Yamagata Bin Zhao Han Ilsoo Tomomi Inoue Naohiro Sata Yoneei Kawaguchi Yoshiro Kubota Tetsuichiro Muto | 2000 | Journal of Hepato - Biliary - Pancreatic Surgery2000，，1： | 1 |
| 5 | Realization of Dynamic Walking and Running of the Quadruped Using Neural Oscillator显示文摘 | Hiroshi Kimura Seiichi Akiyama Kazuaki Sakurama | 1999 | Autonomous Robots1999，，3： | 1 |
| 6 | Realization of Dynamic Walking and Running of the Quadruped Using Neural Oscillator显示文摘 | Hiroshi Kimura Seiichi Akiyama Kazuaki Sakurama | 1999 | Autonomous Robots1999，，3： | 1 |
| 7 | Efficacy of the dual PI3K and mTOR inhibitor NVP-BEZ235 in combination with nilotinib against BCR-ABL-positive leukemia cells involves the ABL kinase domain mutation显示文摘 | Seiichi Okabe Tetsuzo Tauchi Yuko Tanaka Toshihiko Kitahara Shinya Kimura Taira Maekawa Kazuma Ohyashiki | 2014 | Cancer Biology & Therapy2014，，2： | 1 |
| 8 | Embryonic, larval and juvenile development of the roughskin sculpin,Trachidermus fasciatus (Scorpaeniformes: Cottidae)显示文摘 | Naohiko Takeshita Norio Onikura Seiichi Matsui Seir? Kimura | 1997 | Ichthyological Research (-)1997，，2： | 1 |
| 9 | New 9-fluorene-type trispirocyclic compounds for thermally stable hole transport materials in OLEDs显示文摘 | Kimura Makoto Kuwano Seiichi Sawaki Yasuhiko | 2005 | Journal of Materials Chemistry2005，15，24： | 1 |
| 10 | An improved method for the purification of stellate cells from rat liver with Dichloromethylene Diphosphate (CL2MDP)显示文摘 | Yutaka Yata Shin Enosawa Seiichi Suzuki Xiao-Kang Li Akihiko Tamura Hiromitsu Kimura Terumi Takahara Akiharu Watanabe | 1999 | Methods in Cell Science1999，，1： | 1 |
| 11 | Realization of Dynamic Walking and Running of the Quadruped Using Neural Oscillator显示文摘 | Hiroshi Kimura Seiichi Akiyama Kazuaki Sakurama | 1999 | Autonomous Robots1999，，3： | 1 |
| 12 | Embryonic, larval and juvenile development of the roughskin sculpin,Trachidermus fasciatus (Scorpaeniformes: Cottidae)显示文摘 | Naohiko Takeshita Norio Onikura Seiichi Matsui Seir? Kimura | 1997 | Ichthyological Research (-)1997，，2： | 1 |
| 13 | Effect of Catch Cup Geometry on 3D Boundary Layer Flow over the Wafer Surface in a Spin Coating显示文摘最近,当半导体仪器变得更复杂,高技术的开发在生产半导体的进程为薄一致电影的形成被要求了。旋转涂层通常被使用在晶片表面上散布光致抗蚀剂。然而,自从旋转,磁盘的速度在旋转涂层很高,扔的光致抗蚀剂向外散布并且在电影表面上重新依附。钩子杯在纺纱在晶片外面被建立涂层,和散布光致抗蚀剂薄雾被在在晶片边和钩子杯之间的差距产生的 exhaust 流动从晶片边移开。在旋转涂层的干燥过程,是一颗严肃的担心在低旋转的情况中的晶片边附近的电影厚度增加加速。这研究的目的是在晶片表面和液膜的弄干的率上在 3D 界面层流动上弄明白钩子杯几何学的效果。 | Mizue MUNEKATA Seiichi KIMURA Hiroaki KURISHIMA Jinsuke TANAKA Sohei YAMAMOTO Hiroyuki YOSHIKAWA Kazuyoshi MATSUZAKI Hideki OHBA | 2008 | Journal of Thermal Science2008，17，1： | 0 |
| 14 | Influences of the Exhaust Flow on the Boundary Layer Flow on the Wafer Surface in Spin Coating System显示文摘Recently, development of high technology has been required for abe fomaafion of thin uniform film in manufacturing processes of semiconductor as the semiconductor become more sophisticated. Spin coating is usually used for spreading photoresist on a wafer surface. However, since rotating speed of the disk is very high in spin coating, the dropped resist scatters outward and reattaches to the film surface. So, the scattered resist is removed by the exhaust flow generated at the gap between the wafer edge and the catch cup. It is seriously concerned that the stripes called Ekman spiral vortices appears on the disk in the case of high rotating speed and the film thickness increases near the wafer edge in the case of low rotating speed, because it prevent the fomation of uniform film. The purpose of this study is to make clear the generation mechanism of Ekman spiral vortices and the influence of exhaust flow on it. Moreover the influence of the catch cup geometry on the wafer surface boundary layer flow is investigated. | Seiichi KIMURA Mizue MUNEKATA Hiroaki KURISHIMA Kazuyoshi MATSUZAKI Hideki OHBA | 2005 | Journal of Thermal Science2005，14，2： | 0 |
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