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| 1 | Identification of immunodominant linear B-cell epitopes within the major outer membrane protein of Chlamydia trachomatis显示文摘Chlamydia trachomatis 是最流行的性播送的病原体之一。Chlamydial 主要的外部膜蛋白质(MOMP ) 能在鼠科的模型导致强壮的细胞、体液的有免疫力的回答并且被认为是一个潜在的疫苗的候选人。在这份报告, MOMP 的氨基酸顺序用帮助计算机的技术被分析扫描 B 房间 epitopes,和三可能的线性 B 房间 epitopes 肽(VLKTDVNKE, TKDASIDYHE, TRLIDERAAH ) 与高预言的 antigenicity 和高度,保存被调查。为各潜在的 epitope 编码区域的 DNA 被克隆进 pET32a (+) 并且在 Escherichia coli 表示了为 Trx-His-tag 熔化蛋白质。熔化蛋白质被 Ni-NTA agarose 祷告净化并且由 SDS 页和西方的污点分析列在后面。我们与这三熔化蛋白质使老鼠免疫。从使免疫的老鼠包含 anti-epitope 抗体的 sera 能认出 C。在 ELISA 的 trachomatis serovars D 和 E。这些熔化蛋白质的 Antisera 在 serovar E 的侵略上显示了禁止的效果由在 vitro 中立化试金。另外,浆液从恢复健康的 C 取样。感染 trachomatis 的病人与由西方的污点试金的 epitope 熔化蛋白质是反应的。我们的结果证明生物信息的分析选择的 epitope 序列是高度保存的 C。trachomatis MOMP B 房间 epitopes,并且能是子单元疫苗的发展的好候选人,它能在临床的诊断被使用。 | Shanli Zhu Jun Chen Meixia Zheng Wenci Gong Xiangyang Xue Wenshu Li Lifang Zhang | 2010 | Acta Biochimica et Biophysica Sinica2010，42，11： | 11 |
| 2 | Expression of HPV6b L1/EBV LMP2 multiepitope and immunogenicity in mice显示文摘人的乳头状瘤病毒(HPV ) 专业衣壳蛋白质 L1 是为 epitopes 的交货的重要车辆。调查包含 EpsteinBarr 的 multiepitope 的 hybridized HPV6b L1 的表示和 immunogenicity 病毒(EBV ) 潜伏膜蛋白质 2 (LMP2 ) ,包含哺乳动物的 codon 优化 HPV6b L1 基因和 EBV LMP2 multiepitope 的 recombinant plasmid pcDNA3.1 (+) 被构造。包含 T 房间和 B 房间 epitope 富有的肽的 EBV LMP2 multiepitope 被插入到 HPV6b 编码 L1 顺序的 C 终端。构造 plasmid 在由酶限制试金和 DNA 验证了以后,定序是进 COS-7 房间的 transfected。在 COS-7 房间的妄想的基因的表示被 RT-PCR,西方的污点分析和 immunofluorescence 试金证实。结果在 transfected COS-7 房间在 mRNA 和蛋白质层次两个都揭示了妄想的 HPV6b L1/EBV LMP2 multiepitope 基因的成功的表示。在老鼠的肌内的管理能对 HPV6b 得到不仅抗体 L1 像病毒的粒子和 EBV LMP2,而且对 EBV LMP2 epitopes 的细胞毒素的 T 淋巴细胞活动。现在的结果证实那 HPV L1 蛋白质是潜在的作为 immunogen 把 EBV LMP2 的 multiepitope 送到 MHC 一级和班 II 小径,扩大象交货车辆的 HPV L1 的使用。 | Shanli Zhu Xiangyang Xue Jianxiao Liu Lijun Lu Pengyun Zhao Jia Wang WenShu Li Lifang Zhang | 2010 | Acta Biochimica et Biophysica Sinica2010，42，8： | 4 |
| 3 | Construction of van der Waals substrates for largely mismatched heteroepitaxy systems using first principles显示文摘The high density of defects induced by large strains in largely mismatched heteroepitaxy systems, such as AlN and GaN, because of the large lattice and thermal mismatch between substrates and epilayers is the bottleneck problem. Graphene-assisted van der Waals(vdW) heteroepitaxy offers a new opportunity to resolve this problem. However, it suffers from the difficulty of nucleation. Here we theoretically assess the effects of five 2D materials for vdW heteroepitaxy of AlN and GaN, including graphene, hBN, MoS2, gC3N, and gC3N4, and provide physical insights using first-principle calculations. MoS2 and gC3N exhibit significant potential to overcome the shortcomings of graphene owing to their appropriate binding strengths and Al(or Ga)diffusion barriers. Moreover, the interface behavior between the epilayers and the substrates are carefully analyzed. Our findings are helpful not only for obtaining high-quality AlN and GaN films but also for developing new criterions to discover effective 2D materials for vdW heteroepitaxy. | ZhiMing Shi XiaoJuan Sun YuPing Jia XinKe Liu ShanLi Zhang ZhanBin Qi DaBing Li | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，12： | 3 |
| 4 | Polarization-enhanced AlGaN solar-blind ultraviolet detectors显示文摘AlGaN solar-blind ultraviolet detectors have great potential in many fields,although their performance has not fully meet the requirements until now.Here,we proposed an approach to utilize the inherent polarization effect of AlGaN to improve the detector performance.AlGaN heterostructures were designed to enhance the polarization field in the absorption layer,and a high built-in field and a high electron mobility conduction channel were formed.As a result,a high-performance solar-blind ultraviolet detector with a peak responsivity of 1.42 A/W at 10 V was achieved,being 50 times higher than that of the nonpolarization-enhanced one.Moreover,an electron reservoir structure was proposed to further improve the performance.A higher peak responsivity of 3.1 A/W at30 V was achieved because the electron reservoir structure could modulate the electron concentration in the conduction channel.The investigation presented here provided feasible approaches to improve the performance of the AlGaN detector by taking advantage of its inherent property. | Ke Jiang Xiaojuan Sun Zi-Hui Zhang Jianwei Ben Jiamang Che Zhiming Shi Yuping Jia Yang Chen Shanli Zhang Wei Lv Dabing Li | 2020 | Photonics Research2020，8，7： | 3 |
| 5 | Activities of principal photosynthetic enzymes in green macroalga Ulva linza:functional implication of C_4 pathway in CO_2 assimilation显示文摘The green-tide-forming macroalga Ulva linza was profiled by transcriptome sequencing to ascertain whether the alga carries both C3 and C4 photosynthesis genes.The key enzymes involved in C4 metabolism including pyruvate orthophosphate dikinase(PPDK),phosphoenolpyruvate carboxylase(PEPC),and phosphoenolpyruvate carboxykinase(PCK) were found.When measured under normal and different stress conditions,expression of rbcL was higher under normal conditions and lower under the adverse conditions,whereas that of PPDK was higher under some adverse conditions,namely desiccation,high salinity,and low salinity.Both ribulose-1,5-biphosphate carboxylase(RuBPCase) and PPDK were found to play a role in carbon fixation,with significantly higher PPDK activity across the stress conditions.These results suggest that elevated PPDK activity alters carbon metabolism in U.linza leading to partial operation of the C4 carbon metabolism,a pathway that,under stress conditions,probably contributes to the hardy character of U.linza and thus to its wide distribution. | XU JianFang ZHANG XiaoWen YE NaiHao ZHENG Zhou MOU ShanLi DONG MeiTao XU Dong MIAO JinLai | 2013 | Science China(Life Sciences)2013，56，6： | 2 |
| 6 | Epstein-Barr 病毒的从潜伏的膜蛋白质 2 的重叠 epitopes (LMP2 ) 的空想地熔化的抗原富人作为一支潜在的疫苗和诊断代理人显示文摘Epstein-Barr 病毒(EBV ) 在全世界是流行的并且在人与几恶意的疾病被联系。(LMP2 ) 潜伏的膜蛋白质 2 在联系 EBV 的 tumors; 的致病 EBV 起一个关键作用;因此, LMP2 被认为是一个潜在的 immunodiagnostic 和 immunotherapeutic 目标。multi-epitope-based 抗原是为治疗学的疫苗的一种有希望的选择并且如此的恶意诊断。在这研究,我们系统地屏蔽了细胞毒素的 T 淋巴细胞(CTL ) ,助手 T 房间(Th ) 和在用生物信息学的 EBV-LMP2 以内的 B 房间 epitopes。基于屏幕,在 T 房间和 B 房间的重叠 epitopes 的二个肽富人被选择构造为叫作 EBV-LMP2m 的妄想的 multi-epitope 蛋白质包含顺序的 plasmid,它 LMP2aa195~232 和 LMP2aa419~436 是镇静的。EBV-LMP2m 蛋白质在 E 被表示。在原核生物的 codon 优化以后的 coli BL21 (DE3 ) 。在 BALB/c 老鼠的净化的妄想的抗原的接种在阴道粘液而且特定的 CTL 回答在浆液和能分泌的 IgA 导致了特定的 IgG 的高水平不仅。由把净化的 EBV-LMP2m 用作抗原,在 202 鼻咽的癌(NPC ) 的浆液标本的特定的 IgG 的存在病人们有效地与 52.84 % 被检测;敏感和 95.40 %特性,基于 VCA-IgA 在传统的察觉方法上代表改进, 60.53 %敏感和 76.86 %特性) 。上述结果显示 EBV-LMP2m 可以为 NPC 病人为联系 EBV 的肿瘤而且一个诊断代理人被用作潜在的目标抗原不仅。 | Xiaoyun Lin Shao Chen Xiangyang Xue Lijun Lu Shanli Zhu Wenshu Li Xiangmin Chen Xiaozhi Zhong Pengfei Jiang Torsoo Sophia Sename Yi Zheng Lifang Zhang | 2016 | Cellular & Molecular Immunology2016，13，4： | 2 |
| 7 | A multi-epitope vaccine based on Chlamydia trachomatis major outer membrane protein induces specific immunity in mice显示文摘我们评估了包括主要的外部膜蛋白质(MOMP ) 的一支候选人疫苗的 immunogenicity 和功效 multi-epitopeof Chlamydia trachomatis。从包含多重 T 房间和 B 房间 epitopes 的 MOMP 导出的 multi-epitope 的短基因是人工地,包含 codon 的 synthesized.The recombinant plasmid pET32a (+) 优化了 MOMP multi-epitope 基因被构造。在 Escherichia coli 的 fusionprotein Trx-His-MOMP multi-epitope 的表示被钠 dodecyl sulfatepolyacrylamide 胶化电气泳动和西方的污点分析证实。Balb/c 老鼠是 inoculatedwith 皮下地,三与 2 星期的间隔预定的净化的熔化蛋白质。结果出现了 MOMP multi-epitopeelicited 不仅对 C 的强壮的体液的有免疫力的回答。由显著地产生特定的抗体(IgG1 和 IgG2a ) 的高水平的 trachomatis,而且由 inducingrobust 的细胞的有免疫力的回答在老鼠的细胞毒素的 T 淋巴细胞回答。而且, MOMP multi-epitope 实质地告知 IFN- 的分泌物,表明这支疫苗能导致强壮的 Th1 回答。最后,老鼠与 C 的 MOMP multi-epitope displayeda 减小种牛痘。在 chlamydial 挑战和感染的 C 的一个加速的清理之上流的 trachomatis。trachomatis。在结论, MOMP multi-epitope 疫苗可以对 C 为有效预防 andtherapeutic 疫苗的发展有潜力。trachomatis 感染。 | Jianxin Tu B ailong Hou Bingbing Wang Xiaoyun Lin Wenci Gong Haiyan Dong Shanli Zhu Shao Chen Xiangyang Xue Kong-Nan Zhao Lifang Zhang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，5： | 1 |
| 8 | Detection of two exogenous genes in transgenie cattle by loop-mediated isothermal amplification 显示文摘 | Zhai Shanli Liu Chuxin Zhang Qingde | 2012 | Transgenic Res2012，21，6： | 1 |
| 9 | A compact tri - band PIFA antenna for WLAN And WiMAX applications 显示文摘 | Shanli Zuo Zhiya Zhang Wei jun Wu Yingzeng Yin | 2010 | Microwave And Optical Technology Letters2010，52，4： | 1 |
| 10 | Differential physiological responses of the coastal cyanobacterium Synechococcus sp. PCC7002 to elevated pCO2 at lag, exponential,and stationary growth phases显示文摘We studied the effects of expected end-of-the-century p CO_2(1000 ppm)on the photosynthetic performance of a coastal marine cyanobacterium Synechococcus sp.PCC7002 during the lag,exponential,and stationary growth phases.Elevated p CO_2significantly stimulated growth,and enhanced the maximum cell density during the stationary phase.Under ambient p CO_2conditions,the lag phase lasted for 6 days,while elevated p CO_2shortened the lag phase to two days and extended the exponential phase by four days.The elevated p CO_2increased photosynthesis levels during the lag and exponential phases,but reduced them during the stationary phase.Moreover,the elevated p CO_2reduced the saturated growth light(Ik)and increased the light utilization efficiency(α)during the exponential and stationary phases,and elevated the phycobilisome:chlorophyll a(Chl a)ratio.Furthermore,the elevated p CO_2reduced the particulate organic carbon(POC):Chl a and particulate organic nitrogen(PON):Chl a ratios during the lag and stationary phases,but enhanced them during the exponential phase.Overall,Synechococcus showed differential physiological responses to elevated p CO_2during different growth phases,thus providing insight into previous studies that focused on only the exponential phase,which may have biased the results relative to the effects of elevated p CO_2in ecology or aquaculture. | Shanli MOU Gang LI Hongmei LI Fazhong LI Zhisheng SHAO Jinsong LI Changfeng QU Yongyu ZHANG | 2018 | Science China Earth Sciences2018，61，10： | 1 |
| 11 | Reduning Injection prevents carrageenan-induced inflammation in rats by serum and urine metabolomics analysis显示文摘Objective:To elucidate the anti-inflammatory mechanism of Reduning Injection(RDN)by analyzing the potential biomarkers and metabolic pathways of the carrageenan-induced inflammatory model from the overall metabolic level.Methods:Rat inflammatory model was established by carrageenan.UPLC-Q-TOF/MS was used to detect and analyze changes of endogenous metabolites in the serum and urine of carrageenan-induced inflammatory rats.Combined with multivariate analysis and databases analysis,inflammatory-related potential biomarkers were screened and identified to analyze possible metabolic pathways.The reliability and biological significance of these biomarkers was verified by metabolic network analysis and correlation analysis with pharmacodynamic indicators.Results:A total of 16 potential biomarkers were screened and identified by multivariate analysis and metabolite databases,among which 13 species could be adjusted by RDN.The metabolism pathway analysis revealed that histidine metabolism,sphingolipid metabolism,and tyrosine metabolism were greatly disturbed.Their biomarkers involved urocanic acid,sphingosine,and norepinephrine,all of which showed a callback trend after RDN treatment.The three biomarkers had a certain correlation with some known inflammatory-related small molecules(histamine,arachidonic acid,Leukotriene B4,and PGE2)and pharmacodynamic indicators(IL-6,IL-1β,PGE2and TNF-a),which indicated that the selected biomarkers had certain reliability and biological significance.Conclusion:RDN has a good regulation of the metabolic disorder of endogenous components in carrageenan-induced inflammatory rats.And its anti-inflammatory mechanism is mainly related to the regulation of amino acid and lipid metabolism.This research method is conducive to the interpretation of the overall pharmacological mechanism of Chinese medicine. | Xia Gao Jiajia Wang Xialin Chen Shanli Wang Chaojie Huang Quanchang Zhang Liang Cao Zhenzhong Wang Wei Xiao | 2022 | Chinese Herbal Medicines2022，14，4： | 1 |
| 12 | 240 nm AlGaN-based deep ultraviolet micro-LEDs:size effect versus edge effect显示文摘240 nm AlGaN-based micro-LEDs with different sizes are designed and fabricated.Then,the external quantum efficiency(EQE)and light extraction efficiency(LEE)are systematically investigated by comparing size and edge effects.Here,it is revealed that the peak optical output power increases by 81.83%with the size shrinking from 50.0 to 25.0μm.Thereinto,the LEE increases by 26.21%and the LEE enhancement mainly comes from the sidewall light extraction.Most notably,transversemagnetic(TM)mode light intensifies faster as the size shrinks due to the tilted mesa side-wall and Al reflector design.However,when it turns to 12.5μm sized micro-LEDs,the output power is lower than 25.0μm sized ones.The underlying mechanism is that even though protected by SiO2 passivation,the edge effect which leads to current leakage and Shockley-Read-Hall(SRH)recombination deteriorates rapidly with the size further shrinking.Moreover,the ratio of the p-contact area to mesa area is much lower,which deteriorates the p-type current spreading at the mesa edge.These findings show a role of thumb for the design of high efficiency micro-LEDs with wavelength below 250 nm,which will pave the way for wide applications of deep ultraviolet(DUV)micro-LEDs. | Shunpeng Lu Jiangxiao Bai Hongbo Li Ke Jiang Jianwei Ben Shanli Zhang Zi-Hui Zhang Xiaojuan Sun Dabing Li | 2024 | Journal of Semiconductors2024，45，1： | 0 |
| 13 | Research on nodular iron joint welded with nickel-iron electrode by the moire fringe method显示文摘Nickel-iron type cast iron electrodes are widely used for weldingQT600-3 nodular iron.Nevertheless,the research work has demonstrated thatthe strength of these heterogeneous welded joints is low.In this paper,the uni-axial tension of the joints is studied by the Moire fringe method,and the dis-placement,strain and stress in the total fields are obtaind.Based on the unevendistribution of strain and stress,the reason of crack initiation and propagation isdiscussed.Through examining the appearance of the fracture by scanning elec-tron microscope,a lot of spherical substances distributed on the fracture surfaceare found,which may be another reason leading to cracking of the welded jointunder the lower tensile stress.These new findings will help to improve theproperties of nickel-iron type cast iron electrodes and the strength of the weldedjoint. | Zhou Zhenfeng Zhang Xiaoying Guan Liyuan Zhou Gang Wu Shanli Jilin University of Technology,Changchun | 1992 | China Welding1992，2，2： | 0 |
| 14 | Rapid inactivation of human respiratory RNA viruses by deep ultraviolet irradiation from light-emitting diodes on a high-temperatureannealed AlN/Sapphire template显示文摘Efficient and eco-friendly disinfection of air-borne human respiratory RNA viruses is pursued in both public environment and portable usage.The AlGaN-based deep ultraviolet(DUV)light-emission diode(LED)has high practical potentials because of its advantages of variable wavelength,rapid sterilization,environmental protection,and miniaturization.Therefore,whether the emission wavelength has effects on the disinfection as well as whether the device is feasible to sterilize various respiratory RNA viruses under portable conditions is crucial.Here,we fabricate AlGaN-based DUV LEDs with different wavelength on high-temperature-annealed(HTA)AlN/Sapphire templates and investigate the inactivation effects for several respiratory RNA viruses.The AlN/AlGaN superlattices are employed between the template and upper n-AlGaN to release the strong compressive stress(SCS),improving the crystal quality and interface roughness.DUV LEDs with the wavelength of 256,265,and 278 nm,corresponding to the light output power of 6.8,9.6,and 12.5 mW,are realized,among which the 256 nm-LED shows the most potent inactivation effect in human respiratory RNA viruses,including SARS-CoV-2,influenza A virus(IAV),and human parainfluenza virus(HPIV),at a similar light power density(LPD)of~0.8 mW/cm2 for 10 s.These results will contribute to the advanced DUV LED application of disinfecting viruses with high potency and broad spectrum in a portable and eco-friendly use. | Ke Jiang Simeng Liang Xiaojuan Sun Jianwei Ben Liang Qu Shanli Zhang Yang Chen Yucheng Zheng Ke Lan Dabing Li Ke Xu | 2023 | Opto-Electronic Advances2023，6，9： | 0 |
| 15 | Prediction of network public opinion based on bald eagle algorithm optimized radial basis function neural network显示文摘Purpose-In the new era of highly developed Internet information,the prediction of the development trend of network public opinion has a very important reference significance for monitoring and control of public opinion by relevant government departments.Design/methodology/approach-Aiming at the complex and nonlinear characteristics of the network public opinion,considering the accuracy and stability of the applicable model,a network public opinion prediction model based on the bald eagle algorithm optimized radial basis function neural network(BES-RBF)is proposed.Empirical research is conducted with Baidu indexes such as“COVID-19”,“Winter Olympic Games”,“The 100th Anniversary of the Founding of the Party”and“Aerospace”as samples of network public opinion.Findings-The experimental results show that the model proposed in this paper can better describe the development trend of different network public opinion information,has good stability in predictive performance and can provide a good decision-making reference for government public opinion control departments.Originality/value-A method for optimizing the central value,weight,width and other parameters of the radial basis function neural network with the bald eagle algorithm is given,and it is applied to network public opinion trend prediction.The example verifies that the prediction algorithm has higher accuracy and better stability. | Jialiang Xie Shanli Zhang Ling Lin | 2022 | International Journal of Intelligent Computing and Cybernetics2022，15，2： | 0 |
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