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| 1 | A 4-bp Insertion at ZmPLA 1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize显示文摘 | Chenxu Liu Xiang Li Dexuan Meng Yu Zhong Chen Chen Xin Dong Xiaowei Xu Baojian Chen Wei Li Liang Li Xiaolong Tian Haiming Zhao Weibin Song Haishan Luo Qinghua Zhang Jinsheng Lai Weiwei Jin Jianbing Yan Shaojiang Chen | 2017 | Molecular Plant2017,10,3: | 39 |
| 2 | Genome Editing and Double-Fluorescence Proteins Enable Robust Maternal Haploid Induction and Identification in Maize显示文摘 | Le Dong Lina Li Changlin Liu Chenxu Liu Shuaifeng Geng Xinhai Li Changling Huang Long Mao Shaojiang Chen Chuanxiao Xie | 2018 | Molecular Plant2018,11,9: | 22 |
| 3 | Discovery of β-Dihydroagarofuran-Type Sesquiterpenoids from the Leaves of Tripterygium wilfordii with Neuroprotective Activities显示文摘Nine undescribed dihydro-β-agarofuran sesquiterpenoid derivatives(1—9),along with a known analogue(10),were obtained from the leaves of Tripterygium wilfordii.Their gross structures were determined via extensive spectroscopic data,and the absolute configurations were elucidated by means of single-crystal X-ray diffraction analysis and electron circular dichroism(ECD)techniques,which include ECD exciton chirality,octant rule of saturated cyclohexanone and comparison between the experimental and calculated ECD spectra.All the isolated compounds were tested for their neuroprotective activities against H2O2-induced cell injury in human neuroblastoma SH-SY5Y cells.Compounds 5 and 6 improved cell viability by 16.15%and 15.12%compared with the H2O2 treated group at 25μmol·L^(-1),respectively. | Shuhui Dong Bin Lin Xiaobian Xue Ming Bai Xiaoxiao Huang Shaojiang Song | 2021 | Chinese Journal of Chemistry2021,39,2: | 4 |
| 4 | Genome-wide prediction in a hybrid maize population adapted to Northwest China显示文摘Genome-wide prediction is a promising approach to boost selection gain in hybrid breeding.Our main objective was to evaluate the potential and limits of genome-wide prediction to identify superior hybrid combinations adapted to Northwest China.A total of 490 hybrids derived from crosses among 119 inbred lines from the Shaan A and Shaan B heterotic pattern were used for genome-wide prediction of ten agronomic traits.We tested eight different statistical prediction models considering additive(A)effects and in addition evaluated the impact of dominance(D)and epistasis(E)on the prediction ability.Employing five-fold cross validation,we show that the average prediction ability ranged from 0.386 to 0.794 across traits and models.Six parametric methods,i.e.ridge regression,LASSO,Elastic Net,Bayes B,Bayes C and reproducing kernel Hilbert space(RKHS)approach,displayed a very similar prediction ability for each trait and two non-parametric methods(random forest and support vector machine)had a higher prediction performance for the trait rind penetrometer resistance of the third internode above ground(RPR_TIAG).The models of A+D RKHS and A+D+E RKHS were slightly better for predicting traits with a relatively high non-additive variance.Integrating trait-specific markers into the A+D RKHS model improved the prediction ability of grain yield by 3%,from 0.528 to 0.558.Of all 6328 potential hybrids,selection of the top 44 hybrids would lead to a 6%increase in grain yield compared with Zhengdan 958,a commercially successful hybrid variety.In conclusion,our results substantiate the value of genome-wide prediction for hybrid breeding and suggest dozens of promising single crosses for developing high-yielding hybrids for Northwest China. | Guoliang Li Yuan Dong Yusheng Zhao Xiaokang Tian Tobias Würschum Jiquan Xue Shaojiang Chen Jochen C.Reif Shutu Xu Wenxin Liu | 2020 | The Crop Journal2020,8,5: | 4 |
| 5 | Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum显示文摘Doubled haploid(DH) technology is used to obtain homozygous lines in a single generation, a technique that significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but this technique is limited by species and genotype recalcitrance. In vivo haploid induction(HI) through seed is widely and efficiently used in maize and was recently extended to several other crops. Here we show that in vivo HI can be triggered by mutation of DMP maternal haploid inducer genes in allopolyploid(allotetraploid) Brassica napus and Nicotiana tabacum. We developed a pipeline for selection of DMP orthologs for clustered regularly interspaced palindromic repeats mutagenesis and demonstrated average amphihaploid induction rates of2.4% and 1.2% in multiple B. napus and N. tabacum genotypes, respectively. These results further confirmed the HI ability of DMP gene in polyploid dicot crops. The DMP-HI system offers a novel DH technology to facilitate breeding in these crops. The success of this approach and the conservation of DMP genes in dicots suggest the broad applicability of this technique in other dicot crops. | Yu Zhong Yuwen Wang Baojian Chen Jinchu Liu Dong Wang Mengran Li Xiaolong Qi Chenxu Liu Kim Boutilier Shaojiang Chen | 2022 | Journal of Integrative Plant Biology2022,64,6: | 4 |
| 6 | Development of high-oil maize haploid inducer with a novel phenotyping strategy显示文摘Doubled haploid(DH) technology is important in modern maize breeding. Haploid inducers determine the efficiency of both haploid induction and identification. It has taken decades to improve the efficiency,haploid induction rate(HIR), from the ~2% of the ancestor haploid inducer, stock6, to the ~10% of modern haploid inducers. Improvement of kernel oil content(KOC) would further enhance haploid identification efficiency. Using molecular marker-assisted selection, in combine with the number of haploids per ear as phenotypic criterion, we developed a new high-oil haploid inducer line, CHOI4, with a mean HIR of 15.8%and mean KOC of 11%. High KOC of CHOI4 can achieve a mean accuracy greater than 90% in identification of haploids of different backgrounds, with reduced false discovery rates and false negative rates in comparison with the previous high-oil haploid inducer line, CHOI3. Comparison of phenotypic selection strategies suggested that the number of haploids per ear can be used as a phenotyping criterion during haploid inducer line development. CHOI4 could further increase the efficiency of large-scale DH breeding programs with lower cost. | Chenxu Liu Jinlong Li Ming Chen Wei Li Yu Zhong Xin Dong Xiaowei Xu Chen Chen Xiaolong Tian Shaojiang Chen | 2022 | The Crop Journal2022,10,2: | 2 |
| 7 | Integration of genomic selection with doubled-haploid evaluation in hybrid breeding: From GS 1.0 to GS 4.0 and beyond显示文摘DOUBLED-HAPLOID TECHNOLOGY FACES A GREAT CHALLENGE FOR HYBRID BREEDING,Ensuring food security for the ever-growing population is a common mission and a great challenge for agricultural scientists worldwide.Historically,advances in crop breeding and management practices have contributed substantially to crop productivity.Indeed,the substantial increase in global grain yields over the last eight decades is largely due to the adoption of hybrids.However,the rate of increase of hybrid yields began to slow down in the early 2000s,and since then,it has reached a plateau for many crops and regions(http://gffzzfa4d2e2b911e4942sup9p9v5bfppx6nb6.ffgz.tsg.suse.edu.cn).Therefore,we must find solutions to accelerating genetic gain and boost hybrid development,for which developing new breeding technologies provides novel creative opportunities. | Junjie Fu Yangfan Hao Huihui Li Jochen C.Reif Shaojiang Chen Changling Huang Guoying Wang Xinhai Li Yunbi Xu Liang Li | 2022 | Molecular Plant2022,15,4: | 2 |
| 8 | Construction of homozygous diploid potato through maternal haploid induction显示文摘Reinventing the tetraploid potato into a seed-propagated,diploid,hybrid potato would significantly accelerate potato breeding.In this regard,the development of highly homozygous inbred lines is a prerequisite for breeding hybrid potatoes,but self-incompatibility and inbreeding depression present challenges for developing pure inbred lines.To resolve this impediment,we developed a doubled haploid(DH)technology,based on mutagenesis of the potato DOMAIN OF UNKNOWN FUNCTION 679 membrane protein(StDMP)gene.Here,we show that a deficiency in StDMP allows the generation of maternal haploids for generating diploid potato lines.An exercisable protocol,involving hybridization,fluorescent marker screening,molecular and flow cytometric identification,and doubling with colchicine generates nearly 100%homozygous diploid potato lines.This dmp-triggered haploid induction(HI)system greatly shortens the breeding process and offers a robust method for generating diploid potato inbred lines with high purity. | Jinzhe Zhang Jian Yin Jiayi Luo Die Tang Xijian Zhu Jie Wang Zhihong Liu Pei Wang Yu Zhong Chenxu Liu Canhui Li Shaojiang Chen Sanwen Huang | 2022 | aBIOTECH2022,3,3: | 2 |
| 9 | Genkwadane F-I,New Daphnane-type Diterpenes from the Flower Buds of Daphne genkwa Sieb.et Zucc.Exhibit Anti-tumor Activities via lnducing Apoptosis显示文摘of main observation and conclusion Genkwadane F-I(1-4),four new diterpenes,along with four known ones(5-8),were isolated from the flower buds of D.genkwa.The structures including absolute configurations of 1-4 were determined based on the analysis of NMR and ECD calculations.All compounds were evaluated for their cytotoxicities against MCF-7 and Hep3B cell lines,among which compounds 3 and 4 showed significant activities.Compound 4 exhibited the most potent activity with IC50 values of 1.33μM against MCF-7,and the IC50 values of compound 3 against MCF-7 and Hep3B were 5.54μM and 8.73μM;respectively.Further studies by Hoechst 33258 staining and Annexin V/PI analysis demonstrated that compounds 3 and 4 could induce apoptosis to exhibit anti-tumor activities. | Xuewen Hou Jingjie Chen Xuefen Hou Pinyi Gao Jinlin Wang Shaojiang Song Lingzhi Li | 2020 | Chinese Journal of Chemistry2020,38,12: | 1 |
| 10 | Parallel Keyed Hash Function Construction Based on Chaotic Maps显示文摘 | Xiao Di Liao Xiaofeng Deng Shaojiang | 2008 | Physics Letters A2008,372,26: | 1 |
| 11 | A novel key agreement protocol based on chaotic maps显示文摘 | Di Xiao Xiaofeng Liao Shaojiang Deng | 2006 | Information Sciences2006,,4: | 1 |
| 12 | One-way Hash function construction based on the chaotic map with changeable-parameter显示文摘 | Di Xiao Xiaofeng Liao Shaojiang Deng | 2004 | Chaos Solitons and Fractals2004,,1: | 1 |
| 13 | Using time - stamp to Improve the Security of a Chaotic Maps - based Key A- greement Protocol 显示文摘 | Xiao Di Liao Xiaofeng Deng Shaojiang | 2008 | Information Science2008,178,6: | 1 |
| 14 | A Novel Com- bined Cryptographic and Hash Algorithm Based on Chaotic Control Character 显示文摘 | Deng Shaojiang Xiao Di Li Yantao | 2009 | Communications in Nonlinear Sci- ence and Numerical Simulation2009,14,11: | 1 |
| 15 | Effect of thermal annealing on structural electrical and magnetic properties of Ag-doped Lao167Cao: lnO3 thin films grown on LaAIO3 substrates 显示文摘 | Zhang P X Zhu Shaojiang Liu Xiang | 2006 | Japanese Journal of Applied Physics2006,45,: | 1 |
| 16 | Effect of WC content on the microstructure and mechanical properties of Ti(C 0.5 N 0.5 )–WC–Mo–Ni cermets显示文摘 | Jun Qu Weihao Xiong Dameng Ye Zhenghua Yao Wenjun Liu Shaojiang Lin | 2009 | International Journal of Refractory Metals and Hard Materials2009,,2: | 1 |
| 17 | Current and Voltage Mode Resistorless Universal Biquad Filter Using a Single CCCDTA显示文摘Current mode(CM) Single-input and multi-output(SIMO) and Voltage mode(VM) Multi-input and single-output(MISO) biquad filters are proposed. The presented configurations use one Current controlled current differencing transconductance amplifier(CCCDTA)and two capacitors without any external resistors, which is easy suited for integrated circuit fabrication. The currentmode filter can realize the second-order low pass, band pass, high pass and band stop responses simultaneously.And the voltage-mode filter can realize all the standard filtering functions from the same structure. The natural frequency(ω) and quality factor(Q) of the filters can be controlled electronically by transconductance of the CCCDTA. Moreover, the proposed filters does not require any impedance matching due to its two input resistance can be independently tuned through the diverse external bias currents of CCCDTA. The influences of the CCCDTA non-idealities are also analyzed and both the active and passive sensitivities are considerably low. This design was simulated in PSPICE. Simulation results showed that their performances in power, bandwidth, out-band rejection and in-band ripple are better than the traditional. Additionally,the experiment results are provided in the paper. | LIN Shaojiang ZUO Xiaohua DENG Xiangyi | 2018 | Chinese Journal of Electronics2018,27,6: | 1 |
| 18 | Molecular characterization of global maize breeding germplasm based on genome-wide single nucleotide polymorphisms显示文摘 | Yanli Lu Jianbing Yan Claudia T. Guimar?es Suketoshi Taba Zhuanfang Hao Shibin Gao Shaojiang Chen Jiansheng Li Shihuang Zhang Bindiganavile S. Vivek Cosmos Magorokosho Stephen Mugo Dan Makumbi Sidney N. Parentoni Trushar Shah Tingzhao Rong Jonathan H. Cro | 2009 | Theoretical and Applied Genetics2009,,1: | 1 |
| 19 | Bearing Running State recongnition Based on Non-extensive Wave- let Feature Scale Entropy and Support Vetor machine 显示文摘 | DONG Shaojiang TANG Baoping CHEN Renxing | 2013 | Measurement2013,46,10: | 1 |
| 20 | Adaptive Fuzzy Sliding Mode Control of Uncertain Nonlinear SISO Systems显示文摘 | Shaojiang Wang Li Hou Lu Dong Huajun Xiao | 2011 | Procedia Engineering2011,,: | 1 |