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| 1 | Genetic characterization and linkage disequilibrium mapping of resistance to gray leaf spot in maize(Zea mays L.)显示文摘Gray leaf spot(GLS),caused by Cercospora zeae-maydis,is an important foliar disease of maize(Zea mays L.)worldwide,resistance to which is controlled by multiple quantitative trait loci(QTL).To gain insights into the genetic architecture underlying the resistance to this disease,an association mapping population consisting of 161 inbred lines was evaluated for resistance to GLS in a plant pathology nursery at Shenyang in 2010 and 2011.Subsequently,a genome-wide association study,using 41,101 single-nucleotide polymorphisms(SNPs),identified 51 SNPs significantly(P<0.001)associated with GLS resistance,which could be converted into 31 QTL.In addition,three candidate genes related to plant defense were identified,including nucleotidebinding-site/leucine-rich repeat,receptor-like kinase genes similar to those involved in basal defense.Two genic SNPs,PZE-103142893 and PZE-109119001,associated with GLS resistance in chromosome bins 3.07 and 9.07,can be used for marker-assisted selection(MAS)of GLS resistance.These results provide an important resource for developing molecular markers closely linked with the target trait,enhancing breeding efficiency. | Liyu Shi Xiangling Lv Jianfeng Weng Hanyong Zhu Changlin Liu Zhuanfang Hao Yu Zhou Degui Zhang Mingshun Li Xiaoke Ci Xinhai Li Shihuang Zhang | 2014 | The Crop Journal2014，2，Z1： | 9 |
| 2 | Zea mays(L.) P1 locus for cob glume color identified as a post-domestication selection target with an effect on temperate maize genomes显示文摘Artificial selection during domestication and post-domestication improvement results in loss of genetic diversity near target loci. However, the genetic locus associated with cob glume color and the nature of the genomic pattern surrounding it was elusive and the selection effect in that region was not clear. An association mapping panel consisting of 283 diverse modern temperate maize elite lines was genotyped by a chip containing over 55,000 evenly distributed SNPs. Ten-fold resequencing at the target region on 40 of the panel lines and 47 tropical lines was also undertaken. A genome-wide association study(GWAS) for cob glume color confirmed the P1 locus, which is located on the short arm of chromosome 1, with a-log10 P value for surrounding SNPs higher than the Bonferroni threshold(α/n, α < 0.001) when a mixed linear model(MLM) was implemented. A total of 26 markers were identified in a 0.78 Mb region surrounding the P1 locus, including 0.73 Mb and 0.05 Mb upstream and downstream of the P1 gene, respectively. A clear linkage disequilibrium(LD) block was found and LD decayed very rapidly with increasing physical distance surrounding the P1 locus. The estimates of π and Tajima's D were significantly(P < 0.001) lower at both ends compared to the locus. Upon comparison of temperate and tropical lines at much finer resolution by resequencing(180-fold finer than chip SNPs), a more structured LD block pattern was found among the 40 resequenced temperate lines. All evidence indicates that the P1 locus in temperate maize has not undergone neutral evolution but has been subjected to artificial selection during post-domestication selection or improvement. The information and analytical results generated in this study provide insights as to how breeding efforts have affected genome evolution in crop plants. | Chuanxiao Xie Jianfeng Weng Wenguo Liu Cheng Zou Zhuanfang Hao Wenxue Li Minshun Li Xiaosen Guo Gengyun Zhang Yunbi Xu Xinhai Li Shihuang Zhang | 2013 | The Crop Journal2013，1，1： | 6 |
| 3 | Identification of Functional Genetic Variations Underlying Drought Tolerance in Maize Using SNP Markers显示文摘Single nucleotide polymorphism (SNP) is a common form of genetic variation and popularly exists in maize genome. An Illumina GoldenGate assay with 1 536 SNP markers was used to genotype maize inbred lines and identified the functional genetic variations underlying drought tolerance by association analysis. Across 80 lines, 1 006 polymorphic SNPs (65.5% of the total) in the assay with good call quality were used to estimate the pattern of genetic diversity, population structure, and familial relatedness. The analysis showed the best number of fixed subgroups was six, which was consistent with their original sources and results using only simple sequence repeat markers. Pairwise linkage disequilibrium (LD) and association mapping with phenotypic traits investigated under water-stressed and well-watered regimes showed rapid LD decline within 100-500 kb along the physical distance of each chromosome, and that 29 SNPs were associated with at least two phenotypic traits in one or more environments, which were related to drought-tolerant or drought-responsive genes. These drought-tolerant SNPs could be converted into functional markers and then used for maize improvement by marker-assisted selection. | Zhuanfang Hao Xinhai Li Chuanxiao Xie Jianfeng Weng Mingshun Li Degui Zhang Xiaoling Liang Lingling Liu Sisi Liu Shihuang Zhang | 2011 | Journal of Integrative Plant Biology2011，53，8： | 5 |
| 4 | Kernel lysine content does not increase in some maize opaque2 mutants显示文摘 | Gang Zhao Mingshun Li Degui Zhang Xinhai Li Zikai Wu Xiaoke Ci Chuanxiao Xie Li Bai Zhenyu Lu Liang Chen Zhuanfang Hao Shihuang Zhang | 2012 | Planta2012，，1： | 1 |
| 5 | Molecular characterization of global maize breeding germplasm based on genome-wide single nucleotide polymorphisms显示文摘 | Yanli Lu Jianbing Yan Claudia T. Guimar?es Suketoshi Taba Zhuanfang Hao Shibin Gao Shaojiang Chen Jiansheng Li Shihuang Zhang Bindiganavile S. Vivek Cosmos Magorokosho Stephen Mugo Dan Makumbi Sidney N. Parentoni Trushar Shah Tingzhao Rong Jonathan H. Cro | 2009 | Theoretical and Applied Genetics2009，，1： | 1 |
| 6 | The well-shape factor for the measurement of soil hydraulic properties using the Guelph permeameter显示文摘 | Zhuanfang F Zhang Pieter H Groenevelt Gary W Parkin | 1998 | Soil and Tillage Research1998，49，3： | 1 |
| 7 | An analysis of population structure and linkage disequilibrium using multilocus data in 187 maize inbred lines显示文摘 | Chuanxiao Xie Marilyn Warburton Mingshun Li Xinhai Li Muji Xiao Zhuanfang Hao Qi Zhao Shihuang Zhang | 2008 | Molecular Breeding2008，，4： | 1 |
| 8 | The process and mechanism for cesium and rubidium extraction with saponified 4-tert-butyl-2-(a-methylbenzyl) phenol显示文摘Cesium(Cs)and rubidium(Rb)separation from brine is an important and application-oriented topic.4-tert-butyl-2-(a-methylbenzyl)phenol(t-BAMBP)has been used for Cs and Rb extraction.However,the traditional extraction technology is base and acid consumed.In the present work,an innovative process for Cs and Rb extraction with t-BAMBP is developed,which consists of saponification,extraction,scrubbing and stripping.Both infrared spectrum and electrostatic potential analysis indicate the hydrogen of phenolic hydroxyl is dissociated from t-BAMBP during saponification and the oxygen of phenolic hydroxyl is the binding site for alkali metal ions.Saponified organic phase shows an excellent extraction effect for Cs^(+)and Rb^(+).The extraction reaches equilibrium in 5 min,with 99.5%Cs^(+)and 46.7%Rb^(+)are loaded into the organic phase in the single-stage extraction.Slope method indicates the structure of the extraction complex is MOR·3ROH(M=Cs^(+),Rb^(+),K^(+)),where the electrostatic attraction between M^(+)and the oxygen of phenolic hydroxyl is dominant,and the cation–p interaction has a significant effect also.The extraction complex of MOR·3ROH dissociates in the acid environment while scrubbing and stripping is completed.The Cs^(+)and Rb^(+)are separated from the mixture phase,the proton H bonds to the phenolic hydroxyl group,and the extractant is regenerated. | Dengke Pang Zhihong Zhang Yongquan Zhou Zhenhai Fu Quan Li Yongming Zhang Guangguo Wang Zhuanfang Jing | 2022 | Chinese Journal of Chemical Engineering2022，35，6： | 1 |
| 9 | Genomic prediction of yield performance among single-cross maize hybrids using a partial diallel cross design显示文摘Genomic prediction(GP)in plant breeding has the potential to predict and identify the best-performing hybrids based on the genotypes of their parental lines.In a GP experiment,34 elite inbred lines were selected to make 285 single-cross hybrids in a partial-diallel cross design.These lines represented a mini-core collection of Chinese maize germplasm and comprised 18 inbred lines from the Stiff Stalk heterotic group and 16 inbred lines from the Non-Stiff Stalk heterotic group.The parents were genotyped by sequencing and the 285 hybrids were phenotyped for nine yield and yield-related traits at two locations in the summer sowing area(SUS)and three locations in the spring sowing area(SPS)in the main maizeproducing regions of China.Multiple GP models were employed to assess the accuracy of trait prediction in the hybrids.By ten-fold cross-validation,the prediction accuracies of yield performance of the hybrids estimated by the genomic best linear unbiased prediction(GBLUP)model in SUS and SPS were 0.51 and 0.46,respectively.The prediction accuracies of the remaining yield-related traits estimated with GBLUP ranged from 0.49 to 0.86 and from 0.53 to 0.89 in SUS and SPS,respectively.When additive,dominance,epistasis effects,genotype-by-environment interaction,and multi-trait effects were incorporated into the prediction model,the prediction accuracy of hybrid yield performance was improved.The ratio of training to testing population and size of training population optimal for yield prediction were determined.Multiple prediction models can improve prediction accuracy in hybrid breeding. | Ping Luo Houwen Wang Zhiyong Ni Ruisi Yang Fei Wang Hongjun Yong Lin Zhang Zhiqiang Zhou Wei Song Mingshun Li Jie Yang Jianfeng Weng Zhaodong Meng Degui Zhang Jienan Han Yong Chen Runze Zhang Liwei Wang Meng Zhao Wenwei Gao Xiaoyu Chen Wenjie Li Zhuanfang Hao Junjie Fu Xuecai Zhang Xinhai Li | 2023 | The Crop Journal2023，11，6： | 0 |
| 10 | A differentially methylated region of the ZmCCT10 promoter affects flowering time in hybrid maize显示文摘Flowering time(FT) is a key maize domestication trait, variation in which allows maize to grow in a wide range of latitudes. Although previous studies have investigated the genetic control of FT-related traits per se, few studies of FT hybrid performance have been published. We characterized the genomic architecture associated with hybrid performance for FT in a hybrid panel by testcrossing Chang 7–2 with 328Ye478 × Qi319 recombinant inbred lines(RILs). We identified 11 quantitative trait loci(QTL) for hybrid performance in FT-related traits, including a major QTL qFH10 that controls hybrid performance and heterosis in a summer maize-growing region. However, this locus acts in regulating FT traits per se only in a spring maize-growing region. We validated ZmCCT10 as a candidate gene for qFH10 and found that differences between hybrids and their parental lines in DNA methylation in the differentially methylated region(DMR, –700 to –1520) of the ZmCCT10 promoter affected gene expression pattern and thereby FT in the summer maize-growing region. | Zhiqiang Zhou Xin Lu Chaoshu Zhang Mingshun Li Zhuanfang Hao Degui Zhang Hongjun Yong Jienan Han Xinhai Li Jianfeng Weng | 2023 | The Crop Journal2023，11，5： | 0 |
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