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| 1 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 2 | PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by regulating synaptic transmission in cerebellum显示文摘在充满脯氨酸的 transmembrane 蛋白质的变化(PRRT2 ) 2 与发作性的 kinesigenic 运动障碍(PKD ) 和几另外的发作性的神经病学的疾病被联系,但是 PRRT2 功能和病原的机制仍然保持大部分阴暗。这里,我们证明 PRRT2 是与 SNARE 建筑群和 downregulates 的部件交往的 presynaptic 蛋白质它的形成。Loss-of-function 变异的老鼠显示出显型由小脑的概括发作,过高热,或 optogenetic 刺激触发了的象 PKD 一样。有在小脑的小粒房间(GC ) 的特定的 PRRT2 删除的变异的老鼠概括在 Prrt2 空的老鼠看见的行为的显型。而且,记录在显示出的小脑的片做了在短暂举起的 GC 结果的刺激由 Purkinje 房间开火的抑制跟随了的那 optogenetic。在 PKD 处理使用的 anticonvulsant 药 carbamazepine 也在变异的老鼠减轻了象 PKD 一样行为。一起,我们的调查结果作为 SNARE 建筑群的一个新奇管理者识别 PRRT2 并且提供位于 PRRT2 相关的行为下面的电路机制。 | Guo-He Tan Yuan-Yuan Liu Lu Wang Kui Li Ze-Qiang Zhang Hong-Fu Li Zhong-Fei Yang Yang Li Dan Li Ming-Yue Wu Chun-Lei Yu Juan-Juan Long Ren-Chao Chen Li-Xi Li Lu-Ping Yin Ji-Wei Liu Xue-Wen Cheng Qi Shen You-Sheng Shu Kenji Sakimura Lu-Jian Liao Zhi-Ying Wu Zhi-Qi Xiong | 2018 | Cell Research2018，28，1： | 14 |
| 3 | Surgical treatment of nonfunctioning islet cell tumor: report of 41 cases显示文摘BACKGROUND: Nonfunctioning islet cell tumor (NIT)as a rare pancreatic endocrine neoplasm is characterized byunspecific clinical symptoms and is hard to diagnose. InChina, NIT accounts for 15%-41% in pancreatic endocrineneoplasms just next to insulinoma. In this study, weevaluated the surgical modalities of NIT.METHODS: From January 1978 through February 2002, 41patients with NIT were treated at the Department of Sur-gery of the First Affiliated Hospital, China Medical Univer-sity, Shenyang, China. Tumors in the head of the pancreaswere noted in 28 patients, and in the body or in the tail in13 patients. The mean diameter of the tumors was 10. 7cm. Fifteen patients underwent enucleation and 21 receivedpancreatectomy. Tumors were unresectable in 5 patientsbecause of extensive infiltration. The mean diameter was9.6 cm in patients treated by enucleation, 13.1 cm in thoseby pancreaticoduodenectomy, 9.9 cm in those by distalpancreatectomy, and 11.6 cm in those with unresectabletumors.RESULTS: The curative resection rate was 88% (n =36),and the complication rate after enucleation and pancreatec-tomy was 33% ( n = 5 ) and 14% (n=3), respectively. Nolocal recurrence was found after both enucleation and pan-createctomy. Liver metastases occurred in 3 patients treatedby enucleation.CONCLUSIONS: Both enucleation and pancreatectomy areeffective for NIT of the pancreas. No local recurrence hasbeen found in patients treated by the two surgical proce-dures. The complication rates of the two modalities arecomparable. | Ke-Jian Guo, Hai-Hui Liao, Yu-Lin Tian, Ren-Xuan Guo, San-Guang He and Kui Shen Department of Surgery, First Affiliated Hospital, Chi-na Medical University, Shenyang 110001 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，3： | 9 |
| 4 | IL-37b suppresses T cell priming by modulating dendritic cell maturation and cytokine production via dampening ERK/NF-κB/S6K signalings显示文摘Interleukin 37b (IL-37b ) 在压制有免疫力的回答起一个关键作用,部分由 modulating 树枝状的房间(DC ) 的功能。然而,精确机制大部分仍然是未知的。这里,我们在回答由 DC 导致了的 DC 成熟和 T 房间上调查了 IL-37b 的效果,并且探索了深奥发信号的小径。它被发现那 IL-37b 下面调整在 vitro 的 DC 上的 co-stimulatory 分子 CD80 和 CD86 的表情。同时,支持 inflammatory cytokines 的表情例如 TNF-6, 和 IL-6,被压制,当时 T 房间的表示禁止的 cytokine TGF- 在 IL-37b-treated DC 被增加。另外, T 房间上的 DC 的激活效果被 IL-37b 损害。我们进一步表明细胞外的调整单人赛的 kinase (英皇家空军之阶级最低之兵) ,原子 factor-B (NF-B ) ,和表明小径的 mTOR-S6K 涉及 IL-37b 导致的 DC 的抑制。这被化学禁止者的同样镇压的效果在 DC 在 IL-6 和 TNF- 的表情上对 NF-B,英皇家空军之阶级最低之兵,和 S6K 证实。在结论,这些结果证明 IL-37b 由在英皇家空军之阶级最低之兵, NF-B,和基于 S6K 的禁止的发信号小径包含在 T 房间 priming 压制了 DC 成熟和 immunostimulatory 能力。 | Wantong Wu Weiqiang Wang Yun Wang Wenwen Li Gang Yu Zhonglong Li Chunmin Fang Yue Shen Zhina Sun Ling Han Juan Yu Lijun Fang Song Chen Kui Dong Zhongchao Han Hanzhi Liu Yuechen Luo Xiaoming Feng | 2015 | Acta Biochimica et Biophysica Sinica2015，47，8： | 8 |
| 5 | new anastomosis method for choledochojejunostomy by the way behind antrue pyloricum显示文摘 | YANG Xin-wei YANG Jue WANG Kui ZHANG Bao-hua SHEN Feng WU Meng-chao | 2013 | Chinese Medical Journal2013，，24： | 5 |
| 6 | Tuning F-doped degree of rGO:Restraining corrosion-promotion activity of EP/rGO nanocomposite coating显示文摘Given that graphene features high electrical conductivity,it is a kind of material with corrosion-promotion activity.This study aimed to inhibit the corrosion-promotion activity of graphene in coatings.Here,we report an exciting application of epoxy matrix(EP)/F-doped reduced graphene oxide(rGO)coatings for the long-term corrosion protection of steel.The synthesized F-doped rGO(FG)did not reduce the utilization of rGO by a wide margin and possessed distinctive electrically insulating nature.The electrical conductivity of rGO was approximately 1500 S/m,whereas those of FG-1,FG-2 and FG-3 were 1.17,5.217×10^−2 and 3.643×10^-11 S/m,respectively.FG and rGO were then dispersed into epoxy coatings.The chemical structures of rGO and FG were investigated by transmission electron microscopy(TEM),scanning probe microscopy(SPM),X-ray photoelectron spectroscopy(XPS),Fourier-transform infrared spectroscopy(FTIR),and X-ray diffraction(XRD).EP/FG coatings exhibited outstanding corrosion protection in comparison with blank EP and EP/rGO coatings mainly because the corrosion-promotion effect of rGO was eliminated.The anticorrosion ability of EP/FG coatingswasimproved with increased F-doped degree of FG.In addition,electrochemical impendance spectroscopy(EIS)results indicated that the Rc values of EP/FG-2 and EP/FG-3 were four orders of magnitude higher than those of EP/rGO in diluent NaCl solution(3.5 wt.%)after immersion for 90 days. | Lu Shen Yong Li Wenjie Zhao Kui Wang Xiaojing Ci Yangmin Wu Gang Liu Chao Liu Zhiwen Fang | 2020 | Journal of Materials Science & Technology2020，44，9： | 5 |
| 7 | Patients with advanced primary hepatocellular carcinoma treated by melatonin and transcatheter arterial chemoembolization: a prospective study显示文摘Objective: To observe the clinical efficacy of tran-scatheter arterial chemoembolization (TACE) andTACE+MLT (melatonin) on inoperable advancedprimary hepatocellular carcinoma.Methods: From January 1997 to January 1998, onehundred patients with inoperable advanced primaryhepatocellular carcinoma were treated separately byTACE (50) and TACE+MLT (20 mg/d at 8:00PM orally, 7 days before TACE) (50).Results: The effective rates (WHO standards) ofTACE and TACE+MLT were 16% and 28% respec-tively (P<0.05). After TACE or TACE+MLT,the resection rate at two-stage of TACE was 4% or14% (P<0. 01). The 0.5-, 1- and 2-year survivalrates in the TACE group were 82%, 54% and 26%respectively; in the TACE+MLT group 100%, 68%and 40% respectively. The results were significantlybetter in the TACE+MLT group than in the TACEgroup. MLT could protect liver function from thedamage caused by TACE. The IL-2 levels of all pa-tients significantly increased, whereas sIL-2R expres-sions decreased after TACE+MLT as compared withthe TACE group (P<0.01).Conclusions: With definite protection and treatmenteffect on the liver function damage caused by TACE,MLT can enhance the immunological activities of pa-tients. It also can improve the effect of TACE by in-creasing the survival and resection rate after two-stage operation. | Jian-Jun Yan Feng Shen Kui Wang Meng-Chao Wu From the Eastern Hepatobiliary Surgrry Hospital, Shanghai 200438, China | 2002 | Hepatobiliary & Pancreatic Diseases International2002，1，2： | 5 |
| 8 | A Novel Genetic Algorithm for Global Optimization显示文摘This paper presents a novel genetic algorithm for globally solving un-constraint optimization problem.In this algorithm,a new real coded crossover operator is proposed firstly.Furthermore,for improving the convergence speed and the searching ability of our algorithm,the good point set theory rather than random selection is used to generate the initial population,and the chaotic search operator is adopted in the best solution of the current iteration.The experimental results tested on numerical benchmark functions show that this algorithm has excellent solution quality and convergence characteristics,and performs better than some algorithms. | Chun-feng WANG Kui LIU Pei-ping SHEN | 2020 | Acta Mathematicae Applicatae Sinica2020，36，2： | 3 |
| 9 | A novel ionic liquid based on small size cation显示文摘包含小尺寸阳离子的离子的液体材料被综合,它的作文和结构被英尺红外决定, 1 H NMR, 13 C NMR 和元素分析。然而,它象融化那样的物理性质指,粘性,电导率,等等被测量,结果证明新奇离子的液体由小阳离子组成与高度在报导离子的液体家庭之中很稀罕的对称和更大的阴离子。 | Qing Hai Cai Ya Wei Hou Shen Yi Shi Bin Lu Yong Kui Shan | 2009 | Chinese Chemical Letters2009，20，1： | 3 |
| 10 | Design of environmental decision support system and its application to water quality management显示文摘EDSS is a comprehensive software system for water quality management in tidal river networks in general and for the Pearl River Delta in particular. Its purpose is to provide a practical tool that could assist government agencies in decision making for the efficient management of water resources in terms of both quantity and quality. By combining the capabilities of geographical information system (GIS), database management system (DBMS), model base management system (MBMS) and expert system, the aim is to improve the quality of decision making in what is becoming an increasingly complex area. This paper first outlines the basic concepts and philosophy adopted in developing EDSS, the system architecture, design features, implementation techniques and facilities provided. Thereafter, the core part of the system-the hydrodynamic and water quality models are described briefly. The final contribution in this paper describes the application of EDSS to the Pearl River Delta, which has the most complicated tidal river network patterns as well as the fastest economic development in the world. Examples are given of the real-world problems that can be addressed using the system, including cross-boundary water pollution analysis, regional drinking water take-up site selection, screening of important polluters, environmental impact assessment, and water quality zoning and planning. It is illustrated that EDSS can provide efficient and scientific analytical tools for planning and decision-making purposes in the information era. | ZENG Fan-tang, DU Ling, LIN Kui, SHEN Qian (South China Institute of Environmental Sciences, SEPA, Guangzhou 510655, China) | 2001 | Journal of Geographical Sciences2001，11，2： | 2 |
| 11 | Cloning and characterization of miRNAs from maize seedling roots under low phosphorus stress显示文摘 | Zhiming Zhang Haijian Lin Yaou Shen Jian Gao Kui Xiang Li Liu Haiping Ding Guangsheng Yuan Hai Lan Shufeng Zhou Maojun Zhao Shibin Gao Tingzhao Rong Guangtang Pan | 2012 | Molecular Biology Reports2012，，8： | 1 |
| 12 | ,The cure be- havior of tetraglycidyl diaminodiphenyl methane with diaminodiphenyl stdfone 显示文摘 | Gao Jiawu Shen Kui Gao Zongmong | 2000 | Thermochimica Acta2000，，： | 1 |
| 13 | Subsurface damage in optical substrates显示文摘 | Jian Shen Shouhua Liu Kui Yi Hongbo He Jianda Shao Zhengxiu Fan | 2005 | Optik - International Journal for Light and Electron Optics2005，，6： | 1 |
| 14 | Quantitative Analysis of Culture Using Millions of Digitized Books 显示文摘 | Michel Jean -Baptiste Yuan Kui Shen Aviva Presser Aiden et 1 | 2011 | Science2011，，331： | 1 |
| 15 | Subsurface damage in optical substrates 显示文摘 | Jian Shen Shouhu Liu Yi Kui | 2005 | Optik2005，，116： | 1 |
| 16 | Subsurface damage in optical substrates显示文摘 | Jian Shen Shouhua Liu Kui Yi Hongbo He Jianda Shao Zhengxiu Fan | 2005 | Optik - International Journal for Light and Electron Optics2005，，： | 1 |
| 17 | Novel fusiform core-shell-MOF derived in tact metal@carb on composite:An efficient cathode catalyst for aqueous and solid-state Zn-air batteries显示文摘Owing to the varied mechanisms of ORR/OER,exploiting cost-effective bifunctional catalysts with robust ORR/OER activities and excellent performances in Zn-air batteries is still a challenge.In this work,the Co/CoO@NSC bifunctional catalyst is obtained by using Zn-MOF@Co-MOF as self-template.The Co/CoO@NSC composite has interconnected porous architecture with in tact metal@carb on structure,exhibiting superior electrocatalytic activities toward ORR and OER that can be comparable with the Pt/C and RuO_(2) catalysts,respectively.The Co/CoO@NSC-based aqueous Zn-air battery achieves a high specific capacity(759.7 mAh/g)and energy density(990.5 Wh/kg),and ultra-long rechargeable property(more than 400 h/1200 cycles).The Co/CoO@NSC-based solid-state Zn-air battery also delivers an excellent performance with a long cycle life(more than 143 h/858 cycles).Most importantly,the newly synthesized and recharged Co/CoO@NSC-based solid-state Zn-air battery can be used to light up a 2 V LED lamp for more than 28 h,demonstrating the superior practicability as rechargeable power source. | Di Zhou Hongquan Fu Jilan Long Kui Shen Xinglong Gou | 2022 | Journal of Energy Chemistry2022，31，1： | 1 |
| 18 | Liver resection versus liver transplantation for hepatocellular carcinoma within the Milan criteria based on estimated microvascular invasion risks显示文摘Background:Preoperative prediction of microvascular invasion(MVI)in hepatocellular carcinoma(HCC)may optimize individualized treatment decision-making.This study aimed to investigate the prognostic differences between HCC patients undergoing liver resection(LR)and liver transplantation(LT)based on predicted MVI risks.Methods:We analysed 905 patients who underwent LR,including 524 who underwent anatomical resection(AR)and 117 who underwent LT for HCC within the Milan criteria using propensity score matching.A nomogram model was used to predict preoperative MVI risk.Results:The concordance indices of the nomogram for predicting MVI were 0.809 and 0.838 in patients undergoing LR and LT,respectively.Based on an optimal cut-off value of 200 points,the nomogram defined patients as high-or low-risk MVI groups.LT resulted in a lower 5-year recurrence rate and higher 5-year overall survival(OS)rate than LR among the high-risk patients(23.6%vs 73.2%,P<0.001;87.8%vs 48.1%,P<0.001)and low-risk patients(19.0%vs 45.7%,P<0.001;86.5%vs 70.0%,P=0.002).The hazard ratios(HRs)of LT vs LR for recurrence and OS were 0.18(95%confidence interval[CI],0.09–0.37)and 0.12(95%CI,0.04–0.37)among the high-risk patients and 0.37(95%CI,0.21–0.66)and 0.36(95%CI,0.17–0.78)among the low-risk patients.LT also provided a lower 5-year recurrence rate and higher 5-year OS rate than AR among the high-risk patients(24.8%vs 63.5%,P=0.001;86.7%vs 65.7%,P=0.004),with HRs of LT vs AR for recurrence and OS being 0.24(95%CI,0.11–0.53)and 0.17(95%CI,0.06–0.52),respectively.The 5-year recurrence and OS rates between patients undergoing LT and AR were not significantly different in the low-risk patients(19.4%vs 28.3%,P=0.129;85.7%vs 77.8%,P=0.161).Conclusions:LT was superior to LR for patients with HCC within the Milan criteria with a predicted high or low risk of MVI.No significant differences in prognosis were found between LT and AR in patients with a low risk of MVI. | Pinghua Yang Fei Teng Shilei Bai Yong Xia Zhihao Xie Zhangjun Cheng Jun Li Zhengqing Lei Kui Wang Baohua Zhang Tian Yang Xuying Wan Hao Yin Hao Shen Timothy M Pawlik Wan Yee Lau Zhiren Fu Feng Shen | 2023 | Gastroenterology Report2023，11，1： | 1 |
| 19 | Subsurface damage in optical substrates显示文摘 | SHEN Jian LIU Shou-hua YI Kui | 2005 | Optik2005，，116： | 1 |
| 20 | Adjuvant Therapy With Capecitabine Postpones Recurrence of Hepatocellular Carcinoma After Curative Resection: A Randomized Controlled Trial显示文摘 | Yong Xia MD Yinghe Qiu MD Jun Li MD Lehua Shi MD Kui Wang MD Tao Xi MD Feng Shen MD Zhenlin Yan MD Mengchao Wu MD | 2010 | Annals of Surgical Oncology2010，，12： | 1 |
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