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| 1 | Rabdosia serra alleviates dextran sulfate sodium salt-induced colitis in mice through anti-inflammation,regulating Th17/Treg balance,maintaining intestinal barrier integrity,and modulating gut microbiota显示文摘Rabdosia serra(R.serra),an important component of Chinese herbal tea,has traditionally been used to treat hepatitis,jaundice,cholecystitis,and colitis.However,the chemical composition of R.serra and its effect against colitis remain unclear.In this study,the chemical composition of the water extract of R.serra was analyzed using ultra performance liquid chromatography coupled with a hybrid linear ion trap quadrupole-orbitrap mass spectrometer(UPLC-LTQ-Orbitrap-MS).A total of 46 compounds,comprising ent-kaurane diterpenoids,flavonoids,phenolic acids,and steroids,were identified in the water extract of R.serra,and the extract could significantly alleviate dextran sulfate sodium salt-induced colitis by improving colon length,upregulating anti-inflammatory factors,downregulating proinflammatory factors,and restoring the balance of T helper 17/T regulatory cells.R.serra also preserved intestinal barrier function by increasing the level of tight junction proteins(zonula occludens 1 and occludin)in mouse colonic tissue.In addition,R.serra modulated the gut microbiota composition by increasing bacterial richness and diversity,increasing the abundance of beneficial bacteria(Muribaculaceae,Bacteroides,Lactobacillus,and Prevotellaceae_UCG-001),and decreasing the abundance of pathogenic bacteria(Turicibacter,Eubacterium_fissicatena_group,and Eubacterium_xylanophilum_group).Gut microbiota depletion by antibiotics further confirmed that R.serra alleviated colitis in a microbiota-dependent manner.Overall,our findings provide chemical and biological evidence for the potential application of R.serra in the management of colitis. | Hongyi Li Yi Wang Shumin Shao Hui Yu Deqin Wang Chuyuan Li Qin Yuan Wen Liu Jiliang Cao Xiaojuan Wang Haibiao Guo Xu Wu Shengpeng Wang | 2022 | Journal of Pharmaceutical Analysis2022，12，6： | 4 |
| 2 | Solar-Driven Producing of Value-Added Chemicals with Organic Semiconductor-Bacteria Biohybrid System显示文摘Photosynthetic biohybrid systems exhibit promising performance in biosynthesis;however,these systems can only produce a single metabolite and cannot further transform carbon sources into highly valuable chemical production.Herein,a photosynthetic biohybrid system integrating biological and chemical cascade synthesis was developed for solar-driven conversion of glucose to value-added chemicals.A new ternary cooperative biohybrid system,namely bacterial factory,was constructed by self-assembling of enzyme-modified light-harvesting donor-acceptor conjugated polymer nanoparticles(D-A CPNs)and genetically engineered Escherichia coli(E.coli).The D-A CPNs coating on E.coli could effectively generate electrons under light irradiation,which were transferred into E.coli to promote the 37%increment of threonine production by increasing the ratio of nicotinamide adenine dinucleotide phosphate(NADPH).Subsequently,the metabolized threonine was catalyzed by threonine deaminase covalently linking with D-A CPNs to obtain 2-oxobutyrate,which is an important precursor of drugs and chemicals.The 2-oxobutyrate yield under light irradiation is increased by 58%in comparison to that in dark.This work provides a new organic semiconductor-microorganism photosynthetic biohybrid system for biological and chemical cascade synthesis of highly valuable chemicals by taking advantage of renewable carbon sources and solar energy. | Wen Yu Haotian Bai Yue Zeng Hao Zhao Shengpeng Xia Yiming Huang Fengting Lv Shu Wang | 2022 | Research2022，，3： | 2 |
| 3 | Anti-Lung-Cancer Activity and Liposome-Based Delivery Systems of β -Elemene显示文摘 | Meiwan Chen Jinming Zhang Siqin Yu Shengpeng Wang Zaijun Zhang Jianqiang Chen Jian Xiao Yitao Wang Yoshiyuki Kimura | 2012 | Evidence-Based Complementary and Alternative Medicine2012，，： | 1 |
| 4 | Unexpected Photocatalytic Degeneration of NAD^(+) for Inducing Apoptosis of Hypoxia Cancer Cells显示文摘Developing customized chemical reactions that could regulate a specific biological process on demand is regarded as an advanced and promising strategy for treating diseases.However,conventional chemical reactions become challenging in complex physiological environments,which demand mild reaction conditions,high efficiency,good biocompatibility,and strong controllability.Moreover,the effects of the achieved reactions on the real biological system are usually further lessened.Herein,we describe an advanced photocatalytic reaction that irreversibly converted nicotinamide adenine dinucleotide(NAD+)to nicotinamide and adenosine diphosphate(ADP)-ribose by the cationic conjugated poly(fluorene-co-phenylene)(PFP).This reaction was introduced to tumor cells and triggered cell apoptosis.Under white-light illumination,the photocatalytic reaction decreased the NAD+ratio in tumor cells,disrupted the mitochondrial membrane potential,inhibited the synthesis of adenosine triphosphate(ATP),and effectively induced apoptosis.We propose a mechanism of the reaction where PFP is photoexcited to PFP*,and the obtained photoelectrons are transferred from PFP*to NAD+to produce nicotinamide and another unstable intermediate,ADP-ribosyl radical.ADP-ribosyl radical quickly reacts with triethanolamine to form ADP-ribose.This intracellular utilization of a specific photocatalytic reaction could offer a new approach to affect biological function for efficient cancer treatment. | Shengpeng Xia Haotian Bai Endong Zhang Wen Yu Zhiqiang Gao Fengting Lv Yiming Huang Daoben Zhu Shu Wang | 2023 | CCS Chemistry2023，5，10： | 0 |
| 5 | Oxidative stress as a bridge between age and stroke:A narrative review显示文摘Stroke is the third most common cause of death globally and a leading cause of disability.The cellular and molecular changes following stroke and causes of neuronal death are not fully understood,and there are few effective treatments currently available.A rapid increase in the levels of reactive oxygen species(ROS)post stroke can overwhelm antioxidant defenses and trigger a series of pathophysiologic events including the inflammatory response,blood-brain barrier(BBB)disruption,apoptosis,and autophagy,ultimately leading to neuron degeneration and apoptosis.It is thought that beyond a certain age,the RoS accumulation resulting from stroke increases the risk of morbidity and mortality.In the present review,we summarize the role of oxidative stress(OS)as a link between aging and stroke pathogenesis.We also discuss how antioxidants can play a beneficial role in the prevention and treatment of stroke by eliminating harmful ROS,delaying aging,and alleviating damage to neurons. | Shengjie Feng Miaoxian Yang Shengpeng Liu Yu He Shuixiang Deng Ye Gong | 2023 | Journal of Intensive Medicine2023，3，4： | 0 |
| 6 | RESEARCH ARTICLE Open Access Investigating clinical characteristics and prognostic factors in patients with chronic osteomyelitis of humerus显示文摘Background:Chronic osteomyelitis in the humerus,which has complex neuroanatomy and a good soft tissue envelope,represents a unique clinical challenge.However,there are relatively few related studies in the literature.This article retrospectively reviewed a large case series with the aims of sharing our management experiences and further determining factors associated with the outcomes.Methods:Twenty-eight consecutive adult patients with a mean age of 36 years were identified by reviewing the osteomyelitis database of our clinic centre.The database was used to prospectively identify all osteomyelitis cases between 2013 and 2017,and all data then was retrospectively analysed.Results:The mean follow-up period was 35 months(range 24–60).The aetiology was trauma in 43%(12)of the patients and haematogenous in 57%(16)of the patients,and Staphylococcus aureus was a solitary agent in 50%(14)of the patients.Host-type(Cierny’s classification)was IA in 8,IIIB in 11 and IVB in 9 patients.All patients required debridement followed by the placement of a temporary antibiotic-impregnated cement spacer(rod).Seventeen patients received a cement-coated plate for internal fixation after debridement,and 13 patients needed bone grafts when the spacer was staged removed.All patients attained an infection-free bone healing state at the final follow-up.The final average DASH(disabilities of the arm,shoulder and hand)score was 18.14±5.39,while 6 patients(two developed traumatic olecranarthritis,four developed radial nerve injuries)showed the lowest levels of limb function(p=0.000)and were unemployed.Three patients(type I;significant difference between type I versus type III and type IV patients,p<0.05)experienced recurrence after debridement and underwent a second revision,which was not related to the bone graft(p=0.226)or plate fixation(p=0.050).Conclusions:Humeral chronic osteomyelitis can be treated with general surgery and anti-infective therapy;medullary(type I)infection presents a challenge,and the antibiotic-coated cement plate provides favourable fixation without increasing recurrence of infections.Clinicians should be aware of potential iatrogenic nerve injuries when treating these patients with complicated cases,and an experienced surgeon may improve the outcome. | Hongri Wu Shengpeng Yu Jingshu Fu Dong Sun Shulin Wang Zhao Xie Yungui Wang | 2019 | Burns & Trauma2019，7，1： | 0 |
| 7 | Small molecule inhibitors of RORγt for Th17 regulation in inflammatory and autoimmune diseases显示文摘As a ligand-dependent transcription factor,retinoid-associated orphan receptor gt(RORγt)that controls T helper(Th)17 cell differentiation and interleukin(IL)-17 expression plays a critical role in the progression of several inflammatory and autoimmune conditions.An emerging novel approach to the therapy of these diseases thus involves controlling the transcriptional capacity of RORγt to decrease Th17 cell development and IL-17 production.Several RORγt inhibitors including both antagonists and inverse agonists have been discovered to regulate the transcriptional activity of RORγt by binding to orthosteric-or allosteric-binding sites in the ligand-binding domain.Some of small-molecule inhibitors have entered clinical evaluations.Therefore,in current review,the role of RORγt in Th17 regulation and Th17-related inflammatory and autoimmune diseases was highlighted.Notably,the recently developed RORγt inhibitors were summarized,with an emphasis on their optimization from lead compounds,efficacy,toxicity,mechanisms of action,and clinical trials.The limitations of current development in this area were also discussed to facilitate future research. | Jiuping Zeng Mingxing Li Qianyun Zhao Meijuan Chen Long Zhao Shulin Wei Huan Yang Yueshui Zhao Anqi Wang Jing Shen Fukuan Du Yu Chen Shuai Deng Fang Wang Zhuo Zhang Zhi Li Tiangang Wang Shengpeng Wang Zhangang Xiao Xu Wu | 2023 | Journal of Pharmaceutical Analysis2023，13，6： | 0 |
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