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| 1 | Elastic Aerogels of Cellulose Nanofibers@Metal–Organic Frameworks for Thermal Insulation and Fire Retardancy显示文摘Metal–organic frameworks(MOFs)with high microporosity and relatively high thermal stability are potential thermal insulation and flame-retardant materials.However,the difficulties in processing and shaping MOFs have largely hampered their applications in these areas.This study outlines the fabrication of hybrid CNF@MOF aerogels by a stepwise assembly approach involving the coating and cross-linking of cellulose nanofibers(CNFs)with continuous nanolayers of MOFs.The cross-linking gives the aerogels high mechanical strength but superelasticity(80%maximum recoverable strain,high specific compression modulus of^200 MPa cm3 g−1,and specific stress of^100 MPa cm3 g−1).The resultant lightweight aerogels have a cellular network structure and hierarchical porosity,which render the aerogels with relatively low thermal conductivity of^40 mW m−1 K−1.The hydrophobic,thermally stable MOF nanolayers wrapped around the CNFs result in good moisture resistance and fire retardancy.This study demonstrates that MOFs can be used as efficient thermal insulation and flame-retardant materials.It presents a pathway for the design of thermally insulating,superelastic fire-retardant nanocomposites based on MOFs and nanocellulose. | Shengyang Zhou Varvara Apostolopoulou‑Kalkavoura Marcus Vinicius Tavares da Costa Lennart Bergstrom Maria Stromme Chao Xu | 2020 | Nano-Micro Letters2020，12，1： | 10 |
| 2 | Synthesis of monolithic carbon aerogels with high mechanical strength via ambient pressure drying without solvent exchange显示文摘A simple,fast and cost-effective method for monolithic carbon aerogels(CAs) preparation was proposed through sol-gel polycondensation of resorcinol with fo rmaldehyde in a basic aqueous solution followed by ambient pressure drying without solvent exchange,and carbonization.The microstructure and network strength of CAs were tailored by adju sting the catalyst concentration([resorcinol]/[sodium carbonate] in the range of 300-2000),water content([deionized water]/[resorcinol] equals to 17 and 24,respectively),and gelation temperature(Tgel in the range of 30-90℃).Resultantly,the CAs with a wide range of density(0.30-1.13 g/cm3),high specific surface area(465-616 m2/g),high compressive strength(6.5-147.4 MPa)and low thermal conductivity(0.065-0.120 W·m-1 K-1) were obtained in this work.Moreover,the largesized CAs(100×100×20 mm3) can also be prepared by this method since the formed robust skeleton network can resist shrinkage/collapse of pore structure and prevent cracking during drying.The improved mechanical strength and monolithic forming abilities could be mainly attributed to the uniform arrangement of carbon particles and pores,fine particle size,abundant network structure and enhanced particle neck. | Zhi Yang Jian Li Xiaojing Xu Shengyang Pang Chenglong Hu Penglei Guo Sufang Tang Hui-Ming Cheng | 2020 | Journal of Materials Science & Technology2020，47，15： | 4 |
| 3 | N/S co-doped carbon nanosheet bundles as high-capacity anode for potassium-ion battery显示文摘Potassium-ion batteries(PIBs)are of academic and economic significance,but still limited by the lack of highly active electrode materials for de-/intercalation of large-radius K ions.Herein,an interconnected nitrogen/sulfur co-doped carbon nanosheep bundle(N/S-CSB)was proposed as the potassium ions storage material.The rich co-doping of nitrogen/sulfur of N/S-CNB with three-dimensional hierarchical bundled array structure yields distensible interlayer spaces to buffer the volume expansion during K+insertion/extraction,offers more electrochemical active sites to obtain a high specific capacity,and provides efficient channels for fast ion/electron transports.Therefore,the N/S-CSB anode achieved high reversible specific capacity of 365 mAh/g obtained at 50 mA/g after 200 cycles with a coulombic efficiency(CE)close to 100%,high rate performance and long cycle stability.Moreover,the in-situ Raman spectra indicated outstanding reaction kinetics of as-prepared N/S-CSB anode. | Jinhui Cao Jiang Zhong Hanjiao Xu Shengyang Li Hongli Deng Tao Wang Ling Fan Xinghui Wang Lei Wang Jian Zhu Bingan Lu Xidong Duan | 2022 | Nano Research2022，15，3： | 1 |
| 4 | An efficient method for train-track-substructure dynamic interaction analysis by implicit-explicit integration and multi-time-step solution显示文摘In this work,a method is put forward to obtain the dynamic solution efficiently and accurately for a large-scale train-track-substructure(TTS)system.It is called implicit-explicit integration and multi-time-step solution method(abbreviated as mI-nE-MTS method).The TTS system is divided into train-track subsystem and substruc-ture subsystem.Considering that the root cause of low effi-ciency of obtaining TTS solution lies in solving the alge-braic equation of the substructures,the high-efficient Zhai method,an explicit integration scheme,can be introduced to avoid matrix inversion process.The train-track system is solved by implicitly Park method.Moreover,it is known that the requirement of time step size differs for different sub-systems,integration methods and structural frequency response characteristics.A multi-time-step solution is pro-posed,in which time step size for the train-track subsystem and the substructure subsystem can be arbitrarily chosen once satisfying stability and precision demand,namely the time spent for m implicit integral steps is equal to n explicit integral steps,i.e.,mI=nE as mentioned above.The numeri-cal examples show the accuracy,efficiency,and engineering practicality of the proposed method. | Lei Xu Wanming Zhai Shengyang Zhu Weizheng Liu | 2023 | Railway Engineering Science2023，31，1： | 1 |
| 5 | Soft-cuts: A Soft Edge Smoothness Prior for Color Image Super-resolution显示文摘 | Dai Shengyang Han Mei Xu Wei | 2009 | IEEE Transactions on Image Processing2009，18，5： | 1 |
| 6 | Optimaldesign of spatial source-and-relay matrices for a non-regenerative two-way mimo relay system显示文摘 | XU Shengyang HUA Yingbo | 2011 | IEEE Transactions on Wireless Communications2011，10，5： | 1 |
| 7 | SoftCuts : a soft edge smoothness prior for color image super-resolution显示文摘 | Dai Shengyang Han Mei Xu Wei | 2009 | IEEE Transactions on Image Processing2009，18，5： | 1 |
| 8 | SoftCuts: a soft edge smoothness prior for color image super-resolution 显示文摘 | Dai Shengyang Han Mei Xu Wei | 2009 | IEEE Transactions on Image Processing2009，18，5： | 1 |
| 9 | Construction, detection and microarray analysis on Shigella dysenteriae A1 IroN, ShuA single, double mutants显示文摘In this study, we constructed single mutants MTS-1, MTS-2 of IroN and ShuA gene and double mutant MTS of them in Shigella dysenteriae A1 strain 51197 by insert and absence. The functional detection of every mutant was performed at the level of culture medium and cell experiment. The gene expression profiles of the mutants and the wild-type strains under iron- enriched and iron-limited conditions were analyzed by the SD51197 whole genomic microarray. The results showed that all the mutants grew obviously less well than the wild-type strains in L broth appending iron chelator DIP. The addition of iron to the cultures can stimulate the growth of mutants back to wild-type levels. In either the experiments on the ability of intracellular multiplication or the cell-to-cell spread in HeLa and U937 cell lines, mutants showed no obvious change in virulence compared with the parental strain SD51197. However when DIP was added to the cultured HeLa cells, the ability of intracellular multiplication of MTS-1, MTS-2, MTS has reduced about 23.4%, 25.2%, 43.6% respectively. The analysis of expression profiles under the iron-limited condition showed that the mutants were more sensitive for the changes of iron deficiency than the wild-type strains, many genes have been altered. Up-regulated genes mainly involved genes of transcription, coenzyme metabolism, amino acid transport and metabolism, and unknown functional genes, while down-regulated genes mainly involved genes of energy and carbohydrate metabolism and unknown function genes; the expression levels of known iron-transport associated genes generally showed up-regulated. The results demonstrated that iron-transport associated genes IroN, ShuA were likely to have some effects on the virulence and growth of S. dysenteriae. | BIN Wen, LIU Moqing, PENG Junping, SUN Lilian, XU Xingye, ZHANG Jinghai& JIN Qi Shengyang Pharmaceutical University, School of Pharmaceutical Engineering, Shengyang 110016, China State Key Laboratory for Molecular Virology and Genetic Engineering, Beijing 100176, China | 2006 | Science China(Life Sciences)2006，49，3： | 1 |
| 10 | The bacterial effector AvrRxo1 inhibits vitamin B6 biosynthesis to promote infection in rice显示文摘Xanthomonas oryzae pv.oryzicola(Xoc),which causes rice bacterial leaf streak,invades leaves mainly through stomata,which are often closed as a plant immune response against pathogen invasion.How Xoc overcomes stomatal immunity is unclear.Here,we show that the effector protein AvrRxo1,an ATPdependent protease,enhances Xoc virulence and inhibits stomatal immunity by targeting and degrading rice OsPDX1(pyridoxal phosphate synthase),thereby reducing vitamin B6(VB6)levels in rice.VB6 is required for the activity of aldehyde oxidase,which catalyzes the last step of abscisic acid(ABA)biosynthesis,and ABA positively regulates rice stomatal immunity against Xoc.Thus,we provide evidence supporting a model in which a major bacterial pathogen inhibits plant stomatal immunity by directly targeting VB6 biosynthesis and consequently inhibiting the biosynthesis of ABA in guard cells to open stomata.Moreover,AvrRxo1-mediated VB6 targeting also explains the poor nutritional quality,including low VB6 levels,of Xoc-infected rice grains. | Haifeng Liu Chongchong Lu Yang Li Tao Wu Baogang Zhang Baoyou Liu Wenjie Feng Qian Xu Hansong Dong Shengyang He Zhaohui Chu Xinhua Ding | 2022 | Plant Communications2022，3，3： | 1 |
| 11 | Isolation and Identification of Pseudo Seven-Coordinate Ru(Ⅲ)Intermediate Completing the Catalytic Cycle of Ru-bda Type of Water Oxidation Catalysts显示文摘Isolation of RuⅢ-bda(17-electron specie)complex with an aqua ligand(2-electron donor)is challenging due to violation of the 18-electron rule.Although considerable efforts have been dedicated to mechanistic studies of water oxidation by the Ru-bda family,the structure and initial formation of the Ru^(Ⅲ)-bda aqua complex are still controversial.Herein,we challenge this often overlooked step by designing a pocketshape Ru-based complex 1.The computational studies showed that 1 possesses the crucial hydrophobicity at the Ru^(V)(O)state as well as similar probability of access of terminal O to solvent water molecules when compared with classic Ru-bda catalysts.Through characterization of single-crystal structures at the Ru^(Ⅱ) and Ru^(Ⅲ) states,a pseudo seven-coordinate“ready-togo”aqua ligand with Ru^(Ⅲ)…O distance of 3.62A was observed.This aqua ligand was also found to be part of a formed hydrogen-bonding network,providing a good indication of how the Ru^(Ⅲ)-OH_(2)complex is formed. | Tianqi Liu Ge Li Nannan Shen Linqin Wang Brian J.J.Timmer Shengyang Zhou Biaobiao Zhang Alexander Kravchenko Bo Xu Marten S.G.Ahlquist Licheng Sun | 2022 | CCS Chemistry2022，4，7： | 1 |
| 12 | Characteristics of “Guangyuan-Wangcang” trough during Late Middle Permian and its petroleum geological significance in northern Sichuan Basin, SW China显示文摘Based on outcrop characteristics, combined with regional tectonic background, drilling and geophysical data, the sedimentary characteristics of the Middle Permian Maokou Formation in the northern Sichuan Basin was studied by means of macroscopic and microscopic observation, geochemical element test, total organic carbon content and vitrinite reflectance measurement. There is a set of deep-water sediments rich in organic matter in the Guangyuan-Wangcang area of northern Sichuan during the late depositional period of the Middle Permian Maokou Formation. The strata are distributed from northwest to southeast, with thickness of 10–30 m, mainly composed of siliceous rocks and siliceous mudstones, intercalated with gravity flow deposits. Siliceous rocks and siliceous mudstones are characterized by thin single layer, flat bedding and rich siliceous radiolarians, calthrop and brachiopod with small body and thin shell, belonging to the typical sedimentary characteristics of deep-water trough facies. The contents of Cu, Co, Mo, Ni and the ratio of Ni to Co in the geochemical tests all indicate that the siliceous rocks are products of deep-water reducing environment. The TOC value ranges from 3.21% to 8.19%, with an average of 5.53%, indicating that the siliceous rocks have good hydrocarbon generation ability. The south side of the trough is in platform margin facies with high energy, and the sediments are mainly thick massive micritic-calcsparite biogenic(clastic) limestone, which is conducive to the formation and evolution of the reservoir. During the late sedimentary period of the Maokou Formation, the northward subduction and extension of the oceanic crust at the northwestern margin of the Yangtze Plate provided the internal dynamic conditions for the formation of the 'Guangyuan-Wangcang' trough. According to the location, sedimentary characteristics and formation dynamics of the trough, it is similar to the 'Kaijiang-Liangping' trough during Late Permian proposed by previous researchers. It is believed that the 'Kaijiang-Liangping' trough already had its embryonic form during the Late Middle Permian. | WANG Xingzhi LI Bo YANG Xiyan WEN Long XU Liang XIE Shengyang DU Yao FENG Mingyou YANG Xuefei WANG Yaping PEI Senqi | 2021 | Petroleum Exploration and Development2021，48，3： | 0 |
| 13 | Preweaning period is a critical window for rumen microbial regulation of average daily gain in Holstein heifer calves显示文摘Background Rumen bacterial groups can affect growth performance,such as average daily gain(ADG),feed intake,and efficiency.The study aimed to investigate the inter-relationship of rumen bacterial composition,rumen fermentation indicators,serum indicators,and growth performance of Holstein heifer calves with different ADG.Twelve calves were chosen from a trail with 60 calves and divided into higher ADG(HADG,high pre-and post-weaning ADG,n=6)and lower ADG(LADG,low pre-and post-weaning ADG,n=6)groups to investigate differences in bacterial composition and functions and host phenotype.Results During the preweaning period,the relative abundances of propionate producers,including g_norank_f_Butyricicoccaceae,g_Pyramidobacter,and g_norank_f_norank_o_Clostridia_vadin BB60_group,were higher in HADG calves(LDA>2,P<0.05).Enrichment of these bacteria resulted in increased levels of propionate,a gluconeogenic precursor,in preweaning HADG calves(adjusted P<0.05),which consequently raised serum glucose concentrations(adjusted P<0.05).In contrast,the relative abundances of rumen bacteria in post-weaning HADG calves did not exert this effect.Moreover,no significant differences were observed in rumen fermentation parameters and serum indices between the two groups.Conclusions The findings of this study revealed that the preweaning period is the window of opportunity for rumen bacteria to regulate the ADG of calves. | Shengyang Xu Chong Jiao Qiyu Diao Yan Tu | 2024 | Journal of Animal Science and Biotechnology2024，15，1： | 0 |
| 14 | Membrane lipid raft organization during cotton fiber development显示文摘Background:Cotton fiber is a single-celled seed trichome that originates from the ovule epidermis.It is an excellent model for studying cell elongation.Along with the elongation of cotton fiber cell,the plasma membrane is also extremely expanded.Despite progress in understanding cotton fiber cell elongation,knowledge regarding the relationship of plasma membrane in cotton fiber cell development remains elusive.Methods:The plasma membrane of cotton fiber cells was marked with a low toxic fluorescent dye,di-4-ANEPPDHQ,at different stages of development.Fluorescence images were obtained using a confocal laser scanning microscopy.Subsequently,we investigated the relationship between lipid raft activity and cotton fiber development by calculating generalized polarization(GP values)and dual-channel ratio imaging.Results:We demonstrated that the optimum dyeing conditions were treatment with 3μmol·L-1 di-4-ANEPPDHQ for 5 min at room temperature,and the optimal fluorescence images were obtained with 488 nm excitation and500–580 nm and 620–720 nm dual channel emission.First,we examined lipid raft organization in the course of fiber development.The GP values were high in the fiber elongation stage(5–10 DPA,days past anthesis)and relatively low in the initial(0 DPA),secondary cell wall synthesis(20 DPA),and stable synthesis(30 DPA)stages.The GP value peaked in the 10 DPA fiber,and the value in 30 DPA fiber was the lowest.Furthermore,we examined the differences in lipid raft activity in fiber cells between the short fiber cotton mutant,Li-1,and its wild-type.The GP values of the Li-1 mutant fiber were lower than those of the wild type fiber at the elongation stage,and the GP values of 10 DPA fibers were lower than those of 5 DPA fibers in the Li-1 mutant.Conclusions:We established a system for examining membrane lipid raft activity in cotton fiber cells.We verified that lipid raft activity exhibited a low-high-low change regularity during the development of cotton fiber cell,and the pattern was disrupted in the short lint fiber Li-1 mutant,suggesting that membrane lipid order and lipid raft activity are closely linked to fiber cell development. | XU Fan SUO Xiaodong LI Fang BAO Chaoya HE Shengyang HUANG Li LUO Ming | 2020 | Journal of Cotton Research2020，3，2： | 0 |
| 15 | Potassium ion pre‑intercalated MnO_(2)for aqueous multivalent ion batteries显示文摘Manganese dioxide(MnO_(2)),as a cathode material for multivalent ion(such as Mg^(2+)and Al^(3+))storage,is investigated due to its high initial capacity.However,during multivalent ion insertion/extraction,the crystal structure of MnO_(2)partially collapses,leading to fast capacity decay in few charge/discharge cycles.Here,through pre-intercalating potassium-ion(K+)intoδ-MnO_(2),we synthesize a potassium ion pre-intercalated MnO_(2),K_(0.21)MnO_(2)·0.31H_(2)O(KMO),as a reliable cathode material for multivalent ion batteries.The as-prepared KMO exhibits a high reversible capacity of 185 mAh/g at 1 A/g,with considerable rate performance and improved cycling stability in 1 mol/L MgSO_(4)electrolyte.In addition,we observe that aluminum-ion(Al^(3+))can also insert into a KMO cathode.This work provides a valid method for modifcation of manganesebased oxides for aqueous multivalent ion batteries. | Zikang Xu Ruiqi Ren Hang Ren Jingyuan Zhang Jinyao Yang Jiawen Qiu Yizhou Zhang Guoyin Zhu Liang Huang Shengyang Dong | 2023 | Frontiers of Optoelectronics2023，16，4： | 0 |
| 16 | Genomic compositions and phylogenetic analysis of Shigella boydii subgroup显示文摘Comparative Genomic Hybridization (CGH) microarray analysis was used to compare the genomic compositions of all eighteen Shigella boydii serotype representative strains. The results indicated the genomic “backbone” of this subgroup contained 2552 ORFs homologous to nonpatho-genic E. coli K12. Compared with the genome of K12199 ORFs were found to be absent in all S. boydii serotype representatives, including mainly outer membrane protein genes and O-antigen bio-synthesis genes. Yet the specific ORFs of S. boydii subgroup contained basically bacteriophage genes and the function unknown (FUN) genes. Some iron metabolism, transport and type II secretion system related genes were found in most representative strains. According to the CGH phylogenetic analysis, the eighteen S. boydii serotype representatives were divided into four groups, in which se-rotype C13 strain was remarkably distinguished from the other serotype strains. This grouping result corresponded to the distribution of some metabolism related genes. Furthermore, the analysis of genome backbone genes, specific genes, and the phylogenetic trees allowed us to discover the evo-lution laws of S. boydii and to find out important clues to pathogenesis research, vaccination and the therapeutic medicine development. | WANG Jing1*, ZHANG Xiaobing1*, PENG Junping1, YANG E2, BIN Wen3, YANG Jian1, DONG Jie1, SUN Lilian1, XU Xingye1 & JIN Qi1 1. State Key Laboratory for Molecular Virology and Genetic Engineering, Beijing 100176, China 2. School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China 3. School of Pharmaceutical Engineering, Shengyang Pharmaceutical University, Shengyang 110016, China | 2006 | Science China(Life Sciences)2006，49，1： | 0 |
| 17 | Expert consensus on prevention and cardiopulmonary resuscitation for cardiac arrest in COVID-19显示文摘Background:Cardiopulmonary resuscitation(CPR)strategies in COVID-19 patients differ from those in patients suffering from cardiogenic cardiac arrest.During CPR,both healthcare and non-healthcare workers who provide resuscitation are at risk of infection.The Working Group for Expert Consensus on Prevention and Cardiopulmonary Resuscitation for Cardiac Arrest in COVID-19 has developed this Chinese Expert Consensus to guide clinical practice of CPR in COVID-19 patients.Main recommendations:1)A medical team should be assigned to evaluate severe and critical COVID-19 for early monitoring of cardiac-arrest warning signs.2)Psychological counseling and treatment are highly recommended,since sympathetic and vagal abnormalities induced by psychological stress from the COVID-19 pandemic can induce cardiac arrest.3)Healthcare workers should wear personal protective equipment(PPE).4)Mouth-to-mouth ventilation should be avoided on patients suspected of having or diagnosed with COVID-19.5)Hands-only chest compression and mechanical chest compression are recommended.6)Tracheal-intubation procedures should be optimized and tracheal-intubation strategies should be implemented early.7)CPR should be provided for 20-30 min.8)Various factors should be taken into consideration such as the interests of patients and family members,ethics,transmission risks,and laws and regulations governing infectious disease control.Changes in management:The following changes or modifications to CPR strategy in COVID-19 patients are proposed:1)Healthcare workers should wear PPE.2)Hands-only chest compression and mechanical chest compression can be implemented to reduce or avoid the spread of viruses by aerosols.3)Both the benefits to patients and the risk of infection should be considered.4)Hhealthcare workers should be fully aware of and trained in CPR strategies and procedures specifically for patients with COVID-19. | Wei Song Jie Wei Xiangdong Jian Deren Wang Yanhong Ouyang Yuanshui Liu Xianjin Du Ying Chen Yingqi Zhang Heping Xu Shuming Xianyu Qiong Ning Xiang Li Xiaotong Han Feng Zhan Tao Yu Wenteng Chen Jun Zhang Wenwei Cai Sheng’ang Zhou Shengyang Yi Yu Cao Xiaobei Chen Shunjiang Xu Zong’an Liang Duohu Wu Fen Ai Zhong Wang Qingyi Meng Yuhong Mi Sisen Zhang Rongjia Yang Shouchun Yan Wenbin Han Yong Lin Chuanyun Qian Wenwu Zhang Yan Xiong Jun Lv Baochi Liu Yan Cao Xiaojun He Xuelian Sun Yufang Cao Tian’en Zhou | 2021 | Asian Pacific Journal of Tropical Medicine2021，14，6： | 0 |
| 18 | Dynamic response and failure process of horizontal-layered fractured structure rock slope under strong earthquake显示文摘Rock slope with horizontal-layered fractured structure(HLFS)has high stability in its natural state.However,a strong earthquake can induce rock fissure expansion,ultimately leading to slope failure.In this study,the dynamic response,failure mode,and spectral characteristics of rock slope with HLFS under strong earthquake conditions were investigated based on the large-scale shaking table model test.On this basis,multiple sets of numerical calculation models were further established by UDEC discrete element program.Five influencing factors were considered in the parametric study of numerical simulations,including slope height,slope angle,bedding-plane spacing and secondary joint spacing as well as bedrock dip angle.The results showed that the failure process of rock slope with HLFS under earthquake action is mainly divided into four phases,i.e.,the tensile crack of the slope shoulder joints and shear dislocation at the top bedding plane,the extension of vertical joint cracks and increase of shear displacement,the formation of step-through sliding surfaces and the instability,and finally collapse of fractured rock mass.The acceleration response of slopes exhibits elevation amplification effect and surface effect.Numerical simulations indicate that the seismic stability of slopes with HLFS exhibits a negative correlation with slope height and angle,but a positive correlation with bedding-plane spacing,joint spacing,and bedrock dip angle.The results of this study can provide a reference for seismic stability evaluation of weathered rock slopes. | WANG Tong LIU Xianfeng HOU Zhaoxu XU Jiahang ZHANG Jun YUAN Shengyang JIANG Guanlu HU Jinshan | 2024 | Journal of Mountain Science2024，21，3： | 0 |
| 19 | Isolation,identification and artificial inoculation of Ustilago esculenta on Zizania latifolia显示文摘The smut Ustilago esculenta is an endophytic fungus that invades Zizania latifolia(Jiaobai)and plays an important role in inducing the host stem to form a swollen gall.The aims of this study were to investigate isolation,identification and inoculation methods of U.esculenta from Jiaobai.The spore suspension culturing method was the best method of isolating U.esculenta,and yeast-like fungal strains and septate hyphal strains were obtained.The universal primers ITS1 and ITS4 amplified a ribosomal DNA spacer element from all tested strains,while specific primers for Lam16A gene generated a 442 bp product only with septate hyphae strains.De novo transcriptome assembly results showed that the yeast-like fungal strains did not match Ustilago,and genomic analysis suggested the septate hyphae strains were highly similar to known smut fungi.Therefore,the septate hyphae strains were identified as U.esculenta,which indicated that the ITS primers were not species-specific but that Lam16A-specific primers can distinguish U.esculenta accurately and easily.In vitro,U.esculenta grew quickly on PDA medium at 28°C in darkness.Furthermore,an inoculation method was established to produce swollen stems by injecting a spore suspension of U.esculenta at OD600=2.5 into the basal culm internode of male Jiaobai. | Zhiping Zhang Shengyang Xu Mengqi Kong Haibo Dai Yancheng Liu Minmin Miao | 2021 | Horticultural Plant Journal2021，7，4： | 0 |
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