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| 1 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 2 | Nalmefene attenuates malignant potential in colorectal cancer cell via inhibition of opioid receptor显示文摘吗啡被要求在在外科手术前的时期期间支持肿瘤生长和转移的一个风险因素,和肿瘤房间的高 glycolysis 被证明加速肿瘤前进。在这研究,我们调查了是否 nalmefene,一个 opioid 受体禁止者,能通过影响细胞 glycolysis 禁止 CT26 结肠癌细胞生长。CCK8 和 transwell 迁居试金证明 nalmefene 以一种集中依赖者方式禁止了 CT26 房间生存能力和移植。细胞外的使发酸率和氧消费率证明 nalmefene 禁止了 CT26 房间的 glycolysis。而且,西方的污点分析和量的即时 PCR 表明 nalmefene 减少了与 glycolysis 有关的酶的表情。流动 cytometry 结果揭示了那细胞内部的钙(Ca 2+) 水平被 nalmefene 改变,西方的污点分析证明 nalmefene 减少了 calmodulin 表示和 calcium/calmodulin 依赖者蛋白质 kinases II (CaMK II ) phosphorylation,因此禁止 serine/threonine kinase (AKT ) 肝糖 synthase kinase-3 (GSK-3 ) 小径。而且, KN93 的效果, CaMK II 的一个禁止者,类似于 nalmefene,和 nalmefene 的反肿瘤效果的效果能被吗啡抵抗。在结论, nalmefene 的反肿瘤效果可以被禁止 opioid 受体和下面调整的 calmodulin 表示和 CaMK II phosphorylation 完成,因此禁止 AKT-GSK-3 小径和 CT26 房间的 glycolysis。 | Qichao Wu Xiangyuan Chen Jiaqiang Wang Pengfei Sun Meilin Weng Wankun Chen Zhirong Sun Minmin Zhu Changhong Miao | 2018 | Acta Biochimica et Biophysica Sinica2018，50，2： | 2 |
| 3 | Propofol attenuates the adhesion of tumor and endothelial cells through inhibiting glycolysis in human umbilical vein endothelial cells显示文摘Propofol is one of the most commonly used intravenous anesthetics and plays an important role in tumor suppression.In the present study,we aimed to investigate the mechanism by which propofol attenuates tumor endothelial cells(TECs)and tumor cell adhesion to inhibit tumor metastasis in vitro.Human umbilical vein endothelial cells(HUVECs)cultured in Dulbecco’s modified Eagle’s medium were treated with tumor conditioned medium for 24 h,followed by 4 h of treatment with or without 25μM of propofol,10μM of KN93,500μM of MK801,or 20μM of rapastinel.It was found that propofol inhibited TEC adhesion and the glycolysis level of TECs.Consistently,propofol inhibited the expressions of adhesion molecules(E-selectin,ICAM-1,and VCAM-1)and glycolysis proteins(GLUT1,HK2,and LDHA)in TECs.Moreover,propofol attenuated the expression of HIF-1α,the phosphorylation of AKT and Ca2+/calmodulin-dependent protein kinase II(CaMKII),and the Ca2+concentration in TECs.MK801,an inhibitor of NMDA receptor,and KN93,an inhibitor of CaMKII,both inhibited the expressions of adhesion molecules and glycolysis proteins,in a manner similar to propofol.Additionally,rapastine,an activator of NMDA receptor,could counteract the effects of propofol.Our results indicated that propofol attenuates intracellular Ca2+concentration,CaMKII and AKT phosphorylation,and HIF-1αexpression,probably via inhibiting the NMDA receptor,thus inhibiting glycolysis and adhesion of tumor and endothelial cells. | Jie Qi Qichao Wu Xuqin Zhu Shan Zhang Xiangyuan Chen Wankun Chen Zhirong Sun Minmin Zhu Changhong Miao | 2019 | Acta Biochimica et Biophysica Sinica2019，51，11： | 1 |
| 4 | Black phosphorus quantum dots as multifunctional nanozymes for tumor photothermal/catalytic synergistic therapy显示文摘Nanozymes are nanomaterials with enzyme-like properties that have attracted significant interest owing to their high stability,easy preparation,and tunable catalytic properties,especially in the field of cancer therapy.However,the unfavorable catalytic effects of nanozymes in the acidic tumor microenvironment have limited their applications.Herein,we developed a biomimetic erythrocyte membrane-camouflaged ultrasmall black phosphorus quantum dots(BPQDs)nanozymes that simultaneously exhibited an exceptional near-infrared(NIR)photothermal property and dramatically photothermal-enhanced glucose oxidase(GOx)-like activity in the acidic tumor microenvironment.We demonstrated the engineered BPQDs gave a photothermal conversion efficiency of 28.9%that could rapidly heat the tumor up to 50℃ while effectively localized into tumors via homing peptide iRGD leading after intravenously injection.Meanwhile,the significantly enhanced GOx-like activity of BPQDs under NIR irradiation was capable of catalytical generating massive toxic reactive oxygen species via using cellular glucose.By combining the intrinsic photothermal property and the unique photothermal-enhanced GOx-like catalytic activity,the developed BPQDs were demonstrated to be an effective therapeutic strategy for inhibiting tumor growth in vivo.We believe that this work will provide a novel perspective for the development of nanozymes in tumor catalytic therapy. | Hui Ding Daji Wang Haibing Huang Xiaozhu Chen Jie Wang Jinjie Sun Jianlin Zhang Lu Lu Beiping Miao Yanjuan Cai Kelong Fan Yongtian Lu Hongsong Dong Xiyun Yan Guohui Nie Minmin Liang | 2022 | Nano Research2022，15，2： | 1 |
| 5 | Propofol Protects Against High Glucose–Induced Endothelial Apoptosis and Dysfunction in Human Umbilical Vein Endothelial Cells显示文摘 | Minmin Zhu Meilin Wen Xia Sun Wankun Chen Jiawei Chen Changhong Miao | 2015 | Anesthesia & Analgesia2015，，4： | 1 |
| 6 | Erratum to:Black phosphorus quantum dots as multifunctional nanozymes for tumor photothermal/catalytic synergistic therapy显示文摘Erratum to Nano Research 2022,15(2):1554–1563 http://gffzzd3cc09b8251d45dfso9n6xupxxkc660fn.ffgz.tsg.suse.edu.cn/10.1007/s12274-021-3701-8 Figure 3(d)in the original paper contained duplicated micrographs(BPQDs+NIR)for different xenografts(B16 vs.CNE-2).This error did not affect any of the conclusions from the published paper. | Hui Ding Daji Wang Haibing Huang Xiaozhu Chen Jie Wang Jinjie Sun Jianlin Zhang Lu Lu Beiping Miao Yanjuan Cai Kelong Fan Yongtian Lu Hongsong Dong Xiyun Yan Guohui Nie Minmin Liang | 2023 | Nano Research2023，16，1： | 0 |
| 7 | Label-Free,Versatile,Real-Time,and High-Throughput Monitoring of Tyrosine Phosphorylation Based on Reversible Configuration Freeze显示文摘Tyrosine Phosphorylation(pTyr)is a critical and ubiquitous regulation mechanism in biology that plays a central role in controlling intracellular signaling networks.Precise recognition and specific detection of pTyr peptides have been of great importance for both discoveries of disease biomarkers and screening of therapeutic drugs,especially cancers.Here we report a label-free,versatile,realtime,and high-throughput detection strategy for phosphopeptide(PP)based on reversible configuration freeze of a unique hemicyanine-labeled 2-(2′-hydroxyphenyl)-4-methyloxazole(H-HPMO).By taking advantage of the“OFF–ON”transition of fluorescence,H-HPMO–Cu^(2+)complex displays a highly sensitive and selective response to PPs with modified sites on serine,threonine,and tyrosine.Specific recognition of Tyr PPs is achieved by performing a simple logic gate operation and introducing Ca^(2+)interference as an input.This PP detection approach is universal for various peptide sequences and displays high potential in large-scale kinase inhibitor screening,which will promote the development of targeted anticancer drugs. | Yongxin Chang Miao Guo Mengyuan Song Wenjing Sun Dongdong Wang Minmin Li Jixia Wang Yahui Zhang Haijuan Qin Guangyan Qing | 2023 | CCS Chemistry2023，5，6： | 0 |
| 8 | The Optical Properties of Nanostructured Ta_2O_5 Films显示文摘Amorphous Ta_2O_5 films were prepared on Si (100) substrates by thermal oxidization.The film consisted of amorphous Ta_2O_5 nanostructure that grew vertically and compactly at a large range.It was found that Ta_2O_5 films became crystalline when annealed at or above 650℃and remained amorphous below 650℃.The effects of annealing on the optical properties of Ta_2O_5 film were also discussed.It is estimated that the refraction indexes and the optical energy gaps of both amorphous Ta_2O_5 film and crystal one are stable.The optical energy gap of as-deposited Ta_2O_5 film is about 4.81 eV.The above results indicate that Ta_2O_5 films have a promising application in the optical devices. | Minmin Zhu Wei Miao Zhengjun Zhang | 2006 | 稀有金属材料与工程2006，35，A03： | 0 |
| 9 | Simulation of greenhouse cucumber growth,water and nitrogen dynamics in areas with high groundwater(HG)levels using the HG EU-Rotate_N model显示文摘The fruit yield of cucumber are associated with high input of nitrogen,which poses a risk of pollution to the environment.The EU-Rotate_N model has been used widely for its high performance in the simulation of vegetable growth,water and nitrogen dynamics.However,whether the underground water level affects the performance of the EU-Rotate_N model is unclear.In this study,we modified the groundwater level algorithms to the original model and named the modified model'the HG EU-Rotate_N model'.Experiments over two years on greenhouse cucumber with four different nitrogen(N)treatments(N1-N4)were conducted in Jiangsu Province,China,which has a high groundwater level(area_(HG)).Both original and modified models were used to simulate cucumber growth,water movement and N fate.For the soil water content,the measured values were significantly larger than the simulated values of the original model(value_(O))and closer to those of the HG model(value_(HG));for the soil available nitrogen concentration(SNC),the measured values were significantly higher and lower than value_(O) in 0−10 cm and 10−30 cm soil layers,respectively,and were also closer to those of the values_(HG).The higher SNC in the 0-10 cm soil layer indicated that a high groundwater level might increase the upwards movement of water and nitrogen in the 0-30 cm soil layer.The root mean square error,Nash Sutcliffe Efficiency and difference values show that the HG model was more applicable for areaHG than the original model.In this study,the nitrogen dosage of the N3 treatment was sufficient to meet the requirements of cucumber growth,indicating that the fertilization recommendation according to nitrogen nutrient balance was applicable in this area. | Bing Hua Zhuangzhuang Cao Kefeng Zhang Xiangying Xu Yonglong Zhang Haibo Dai Zhiping Zhang Jiezeng Jiang Minmin Miao | 2022 | Vegetable Research2022，2，1： | 0 |
| 10 | Isolation,identification and artificial inoculation of Ustilago esculenta on Zizania latifolia显示文摘The smut Ustilago esculenta is an endophytic fungus that invades Zizania latifolia(Jiaobai)and plays an important role in inducing the host stem to form a swollen gall.The aims of this study were to investigate isolation,identification and inoculation methods of U.esculenta from Jiaobai.The spore suspension culturing method was the best method of isolating U.esculenta,and yeast-like fungal strains and septate hyphal strains were obtained.The universal primers ITS1 and ITS4 amplified a ribosomal DNA spacer element from all tested strains,while specific primers for Lam16A gene generated a 442 bp product only with septate hyphae strains.De novo transcriptome assembly results showed that the yeast-like fungal strains did not match Ustilago,and genomic analysis suggested the septate hyphae strains were highly similar to known smut fungi.Therefore,the septate hyphae strains were identified as U.esculenta,which indicated that the ITS primers were not species-specific but that Lam16A-specific primers can distinguish U.esculenta accurately and easily.In vitro,U.esculenta grew quickly on PDA medium at 28°C in darkness.Furthermore,an inoculation method was established to produce swollen stems by injecting a spore suspension of U.esculenta at OD600=2.5 into the basal culm internode of male Jiaobai. | Zhiping Zhang Shengyang Xu Mengqi Kong Haibo Dai Yancheng Liu Minmin Miao | 2021 | Horticultural Plant Journal2021，7，4： | 0 |
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