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| 1 | A Novel Chimeric Mitochondrial Gene Confers Cytoplasmic Effects on Seed Oil Content in Polyploid Rapeseed (Brassica napus)显示文摘Cytoplasmic effects (CEs) have been discovered to influence a diverse array of agronomic traits in crops, and understanding the underlying mechanisms can help accelerate breeding programs. Seed oil content (SOC) is of great agricultural, nutritional, and economic importance. However, the genetic basis of CEs on SOC (CE-SOC) remains enigmatic. In this study, we use an optimized approach to sequence the cytoplasmic (plastid and mitochondrial) genomes of allotetraploid oilseed rape (Brassica napus) cultivars, 51218 and 56366, that bear contrasting CE-SOC. By combining comparative genomics and genome-wide transcriptome analysis, we identify mitochondria-encoded orf188 as a potential CE-SOC determinant gene. Functional analyses in the model system Arabidopsis thaliana and rapeseed demonstrated that orf188 governs CE-SOC and could significantly increase SOC, strikingly, through promoting the yield of ATP. Consistent with this finding, transcriptional profiling with microarray and RNA sequencing revealed that orf188 affects transcriptional reprogramming of mitochondrial energy metabolism to facilitate ATP production. Intriguingly, orf188 is a previously uncharacterized chimeric gene, and the presence of this genetic novelty endows rapeseed with positive CE-SOC. Our results shed light on the molecular basis of CEs on a key quantitative trait in polyploid crops and enrich the theory of maternal control of oil content, providing new scientific guidance for breeding high-oil rapeseed germplasms. | Jun Liu Wanjun Hao Jing Liu Shihang Fan Wei Zhao Linbing Deng Xinfa Wang Zhiyong Hu Wei Hua Hanzhong Wang | 2019 | Molecular Plant2019，12，4： | 5 |
| 2 | QTL mapping for grain number per spikelet in wheat using a high-density genetic map显示文摘Grain number per spikelet (GNS) is a key determinant of grain yield in wheat.A recombinant inbred line population comprising 300 lines was developed from a cross between a high GNS variety H461 and Chinese Spring from which the reference genome assembly of bread wheat was obtained.Both parents and the recombinant inbred lines were genotyped using the wheat 55K single nucleotide polymorphism(SNP) array.A high-density genetic map containing 21,197 SNPs was obtained.These markers covered each of the 21 chromosomes with a total linkage distance of 3792.71 c M.Locations of these markers in this linkage map were highly consistent with their physical locations in the genome assembly of Chinese Spring.The two parents and the whole RIL population were assessed for GNS in two consecutive years at two different locations.Based on multi-environment phenotype data and best liner unbiased prediction values,three quantitative trait loci (QTL) for GNS were identified.One of them located on chromosomes 2B and the other two on 2D.Phenotypic variation explained by these loci varied from 3.07%to26.57%.One of these QTL,QGns.sicau-2D-2,was identified in each of all trials conducted.Based on the best linear unbiased prediction values,this locus explained 19.59%–26.57%of phenotypic variation.A KASP(Kompetitive Allele-Specific PCR) marker closely linked with this locus was generated and used to validate the effects of this locus in three different genetic backgrounds.The identified QTL and the KASP marker developed for it will be highly valuable in fine-mapping the locus and in exploiting it for markerassisted selection in wheat breeding programs. | Yu Lin Xiaojun Jiang Haiyan Hu Kunyu Zhou Qing Wang Shifan Yu Xilan Yang Zhiqiang Wang Fangkun Wu Shihang Liu Caixia Li Mei Deng Jian Ma Guangdeng Chen Yuming Wei Youliang Zheng Yaxi Liu | 2021 | The Crop Journal2021，9，5： | 4 |
| 3 | Output Prediction of Helical Microfiber Temperature Sensors in Cycling Measurement by Deep Learning显示文摘The inconsistent response curve of delicate micro/nanofiber(MNF)sensors during cycling measurement is one of the main factors which greatly limit their practical application.In this paper,we proposed a temperature sensor based on the copper rod-supported helical microfiber(HMF).The HMF sensors exhibited different light intensity-temperature response relationships in single-cycle measurements.Two neural networks,the deep belief network(DBN)and the backpropagation neural network(BPNN),were employed respectively to predict the temperature of the HMF sensor in different sensing processes.The input variables of the network were the sensor geometric parameters(the microfiber diameter,wrapped length,coiled turns,and helical angle)and the output optical intensity under different working processes.The root mean square error(RMSE)and Pearson correlation coefficient(R)were used to evaluate the predictive ability of the networks.The DBN with two restricted Boltzmann machines(RBMs)provided the best temperature prediction results(RMSE and R of the heating process are 0.9705℃and 0.9969,while the values of RMSE and R of the cooling process are 0.7866℃and 0.9977,respectively).The prediction results obtained by the optimal BPNN(five hidden layers,10 neurons in each layer,RMSE=1.1266℃,R=0.9957)were slightly inferior to those obtained by the DBN.The neural network could accurately and reliably predict the response of the HMF sensor in cycling operation,which provided the possibility for the flexible application of the complex MNF sensor in a wide sensing range. | Minghui CHEN Jinjin HAN Juan LIU Fangzhu ZHENG Shihang GENG Shimeng TANG Zhijun WU Jixiong PU Xining ZHANG Hao DAI | 2023 | Photonic Sensors2023，13，3： | 0 |
| 4 | Effective method for isolating liquid endosperm and improved procedure for subsequent DNA extraction in Brassica napus显示文摘 | Jun LI Shihang FAN Xingchao SUN Zhiyong HU Jing LIU Xiaoyi ZHU Ming ZHENG Fengming SUN Hongli YANG Liang ZHANG Linbin DENG Hongfang LIU Wei HUA | 2016 | Oil Crop Science2016，1，2： | 0 |
| 5 | Synthesis and characteristic of a novel green fluorescent protein eYGFPuv显示文摘Recently, a novel green fluorescent protein eYGFPuv has been identified in the marine organism Chiridius poppei which displays high fluorescence intensity and can be visible by eyes in dark. Although strong green fluorescence was achieved in transgenic petunia, 3 expression cassettes (about 8 kb) complicate its application. In this study, to confirm whether 1 expression cassette could be used as a transgenic marker in prokaryotes and eukaryotes, eYGFPuv was cloned into prokaryotic expression vector pET28α-eYGFPuv- His and plant binary expression vector 35S::eYGFPuv. Compared to EGFP, eYGFPuv protein exhibited stronger dazzling green fluorescence in E. coli under excited light at 365 nm and maintains steadily over a long period of time without degradation. When transiently expressed in tobacco leaves, eYGFPuv protein displayed strong green fluorescence. Moreover, the fluorescence of eYGFPuv protein also could be directly observed in living plant, and thus can be used easily as a marker to screen transformed lines in transgenic research. Overall, compared to previous studies on eYGFPuv tandem repeats, our data confirmed that single eYGFPuv sequence still possesses high fluorescence intensity and quenching resistance. Furthermore, because of small size of expression cassette,it is suitable for efficient transformation in both prokaryotic and eukaryotic organisms. | Shihang Fan Linbin Deng Hongli Yang Liang Zhang Xingchao Sun Jinglin Liu Jing Liu | 2019 | Oil Crop Science2019，4，2： | 0 |
| 6 | Superconductivity in chromium nitrides Pr3Cr10-xN11 with strong electron correlations显示文摘Exploration of superconductivity in Cr-based compounds has attracted considerable interest because only a few Cr-based superconductors(CrAs,A2Cr3As3 and ACr3As3(A=K,Rb,Cs,Na))have been discovered so far and they show an unconventional pairing mechanism.We report the discovery of bulk superconductivity at 5.25 K in chromium nitride in Pr3Cr10-xN11 with a cubic lattice structure.A relatively large upper critical field of Hc2(0)~12.6 T is determined,which is larger than the estimated Pauli-paramagnetic pair-breaking magnetic field.The material has a large electronic specific-heat coefficient of 170 mJ K^-2mol^-1-about 10 times larger than that estimated by the electronic structure calculation,which suggests that correlations between 3d electrons are very strong in Pr3Cr10-xN11,and thus quantum fluctuations might be involved.Electronic structure calculations show that the density of states at the Fermi energy are contributed predominantly by Cr 3d electrons,implying that the superconductivity results mainly from the condensation of Cr 3d electrons.Pr3Cr10-xN11 represents a rare example of possible unconventional superconductivity emerging in a 3D system with strong electron correlations.Nevertheless,clarification of the specific pairing symmetry needs more investigation. | Wei Wu Kai Liu Yanjie Li Zhenhai Yu Desheng Wu Yuting Shao Shihang Na Gang Li Ruizhen Huang Tao Xiang Jianlin Luo | 2020 | National Science Review2020，7，1： | 0 |
| 7 | Spatial variability and driving factors of soil multifunctionality in drylands of China显示文摘Drylands are highly vulnerable to climate change and human activities.The drylands of China account for approximately 10.8%of global drylands,and China is the country most severely affected by aridity in Asia.Therefore,studying the spatial variation characteristics in soil multifunctionality(SMF)and investigating the driving factors are critical for elucidating and managing the functions of dryland ecosystems in China.Based on the environmental factors(mean annual precipitation(MAP),mean annual temperature(MAT),solar radiation(Srad),soil acidity(pH),enhanced vegetation index(EVI),and cation exchange capacity(CEC))and aridity from the“dataset of soil properties for land surface modeling over China”,we used non-linear regression,ordinary least square(OLS)regression,structural equation model(SEM),and other analytical methods to investigate the relationships of SMF with environmental factors across different aridity levels in China.SMF in different dryland regions varied significantly and showed a patchy distribution,with SMF index values ranging from–1.21 to 2.42.Regions with SMF index values from–0.20 to 0.51 accounting for 63.0%of dryland area in China.OLS regression results revealed that environmental factors like MAP,MAT,Srad,pH,EVI,and CEC were significantly related to SMF(P<0.05).MAP and MAT were correlated to SMF at the whole aridity level(P<0.05).SEM results showed that the driving factors of SMF differed depending on the aridity level.Soil pH was the strongest driving factor of SMF when the aridity was less than 0.80(P<0.001).Both soil CEC and EVI had a positive effect on SMF when aridity was greater than 0.80(P<0.01),with soil CEC being the strongest driving factor.The importance ranking revealed that the relative importance contribution of soil pH to SMF was greatest when aridity was less than 0.80(66.9%).When aridity was set to greater than 0.80,the relative importance contributions of CEC and EVI to SMF increased(45.1%and 31.9%,respectively).Our findings indicated that SMF had high spatial heterogeneity in drylands of China.The aridity threshold controlled the impact of environmental factors on SMF. | ZHANG Shihang CHEN Yusen LU Yongxing GUO Hao GUO Xing LIU Chaohong ZHOU Xiaobing ZHANG Yuanming | 2022 | Regional Sustainability2022，3，3： | 0 |
| 8 | Normalization of Indexed Differentials by Extending Grobner Basis Theory显示文摘It is a fundamental problem to determine the equivalence of indexed differential polynomials in both computer algebra and differential geometry.However,in the literature,there are no general computational theories for this problem.The main reasons are that the ideal generated by the basic syzygies cannot be finitely generated,and it involves eliminations of dummy indices and functions.This paper solves the problem by extending Grobner basis theory.The authors first present a division of the set of elementary indexed differential monomials E■ into disjoint subsets,by defining an equivalence relation on E■ based on Leibniz expansions of monomials.The equivalence relation on E■also induces a division of a Grobner basis of basic syzygies into disjoint subsets.Furthermore,the authors prove that the dummy index numbers of the sim-monomials of the elements in each equivalence class of E■ have upper bounds,and use the upper bounds to construct fundamental restricted rings.Finally,the canonical form of an indexed differential polynomial proves to be the normal form with respect to a subset of the Grobner basis in the fundamental restricted ring. | LIU Jiang NI Feng SONG Shihang DU Mingjun | 2022 | Journal of Systems Science & Complexity2022，35，5： | 0 |
| 9 | Spatio-temporal Changes and Associated Uncertainties of CENTURYmodelled SOC for Chinese Upland Soils, 1980-2010显示文摘Detailed information on the spatio-temporal changes of cropland soil organic carbon(SOC) can significantly contribute to the improvement of soil fertility and mitigate climate change. Nonetheless, information and knowledge on the national scale spatio-temporal changes and the corresponding uncertainties of SOC in Chinese upland soils remain limited. The CENTURY model was used to estimate the SOC storages and their changes in Chinese uplands from 1980 to 2010. With the Monte Carlo method, the uncertainties of CENTURY-modelled SOC dynamics associated with the spatial heterogeneous model inputs were quantified. Results revealed that the SOC storage in Chinese uplands increased from 3.03(1.59 to 4.78) Pg C in 1980 to 3.40(2.39 to 4.62) Pg C in 2010. Increment of SOC storage during this period was 370 Tg C, with an uncertainty interval of –440 to 1110 Tg C. The regional disparities of SOC changes reached a significant level, with considerable SOC accumulation in the Huang-Huai-Hai Plain of China and SOC loss in the northeastern China. The SOC lost from Meadow soils, Black soils and Chernozems was most severe, whilst SOC accumulation in Fluvo-aquic soils, Cinnamon soils and Purplish soils was most significant. In modelling large-scale SOC dynamics, the initial soil properties were major sources of uncertainty. Hence, more detailed information concerning the soil properties must be collected. The SOC stock of Chinese uplands in 2010 was still relatively low, manifesting that recommended agricultural management practices in conjunction with effectively economic and policy incentives to farmers for soil fertility improvement were indispensable for future carbon sequestration in these regions. | LIU Xiaoyu ZHAO Yongcun SHI Xuezheng WANG Shihang FENG Xiang YAN Fang | 2021 | Chinese Geographical Science2021，31，1： | 0 |
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