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| 1 | Tissue clearing of both hard and soft tissue organs with the PEGASOS method显示文摘织物空地技术由转织物在 3 尺寸(3-D ) 启用不透明的机关和纸巾的可视化透明。当前的组织空地方法被能与每个单个协议被清除的纸巾的有限类型限制,它不可避免地导致了存在在整个身体或身体部分成像以内盲点。包括骨头和牙齿的难纸巾仍然是要清除的最困难的机关。另外,内长的荧光的损失为基于溶剂的空地方法仍然是一颗主要担心。这里,我们发展了聚乙烯乙二醇(木钉) 联系了溶剂系统(PEGASOS ) ,它将近显示了纸巾的所有类型透明、保存的内长的荧光。骨头和牙齿能被转在变清以后将近不可见。PEGASOS 方法转了透明的整个成年老鼠身体,我们对一个成年老鼠头骨头,牙齿,大脑,肌肉,和其它填写了的图象有能力没有盲目区域的纸巾。难织物透明性使我们能重建在 3-D 的未经触动的上颚,牙齿,腿节,或膝关节。另外,我们在长距离上在亚 cellular 分辨率并且到踪迹个人神经原和轴突管理了未经触动的鼠标大脑到图象。我们也通过 vertebrae 直接设想了背面的根中心。最后,我们在长骨头的髓空间以内在 3-D 揭示了神经网络的分发模式。这些结果建议 PEGASOS 方法是为一般生物医学的研究的一个有用工具。 | Dian Jing Shiwen Zhang Wenjing Luo Xiaofei Gao Yi Men Chi Ma Xiaohua Liu Yating Yi Abhijit Bugde Bo O. Zhou Zhihe Zhao Quan Yuan Jian Q. Feng Liang Gao Woo-Ping Ge Hu Zhao | 2018 | Cell Research2018，28，8： | 18 |
| 2 | PICK1 promotes caveolin-dependent degradation of TGF-β typel receptor显示文摘与 C kinase 交往的蛋白质(PICK1 ) 1 是 α 的一个批评调停人;在神经触处的 -amino-3-hydroxy-5-methyl-4-isoxazolepropionic 酸受体(AMPAR ) trafficking。然而,它的无所不在的表达式建议它可以有另外的非神经的函数。这里,我们证明 PICK1 反对转变生长因素贝它(TGF-β) 由指向 TGF-β 发信号;打字我受体(Tβ RI ) 为降级。生物化学的分析表明 PICK1 直接与 Tβ 的 C 终点交往;经由它是的 PDZ 领域和行为的 RI 提高在 Tβ 之间的相互作用的支架蛋白质; RI 和 caveolin-1,导致提高了类脂化合物 raft/caveolae 本地化。因此, PICK1 增加 Tβ 的调停 caveolin 的 endocytosis, ubiquitination 和降级; RI。而且,在 PICK1 表示和 Tβ 之间的否定关联; RI 或 phospho-Smad2 层次在人的胸肿瘤被观察,显示那 PICK1 可以通过 TGF-β 的抑制参予乳癌开发;发信号。我们的调查结果作为 TGF-β 的一个重要否定管理者揭示 PICK1 的非神经的功能;发信号。 | Bing Zhao Qiang Wang Jun Du Shiwen Luo Jun Xia Ye-Guang Chen | 2012 | Cell Research2012，22，10： | 9 |
| 3 | Hedgehog signaling pathway and ovarian cancer显示文摘Epithelial ovarian carcinoma (EOC) is the most common form of ovarian malignancies and the most lethal gynecologic malignancy in the United States. To date, in spite of treatment to it with the extensive surgical debulking and chemotherapy, the prognosis of EOC remains dismal. Recently, it has become increasingly clear that in many instances, the signaling and molecular players that control development are the same, and when inappropriately regulated, drive tumorigenesis and cancer development. Here, we discuss the possible involvement of Hedgehog (Hh) pathway in the cellular regulation and development of cancer in the ovaries. Using the in vitro and in vivo assays developed has facilitated the dissection of the mechanisms behind Hh-driven ovarian cancers formation and growth. Based on recent studies, we propose that the inhibition of Hh signaling may interfere with spheroid-like structures in ovarian cancers. The components of the Hh signaling may provide novel drug targets, which could be explored as crucial combinatorial strategies for the treatment of ovarian cancers. | Qi Chen Guolan Gao Shiwen Luo | 2013 | Chinese Journal of Cancer Research2013，25，3： | 9 |
| 4 | Cancer-associated adipocyte-derived G-CSF promotes breast cancer malignancy via Stat3 signaling显示文摘Adipocyte is the most predominant cell type in the tumor microenvironment of breast cancer and plays a pivotal role in cancer progression,yet the underlying mechanisms and functional mediators remain elusive.We isolated primary preadipocytes from mammary fat pads of human breast cancer patients and generated mature adipocytes and cancer-associated adipocytes(CAAs)in vitro.The CAAs exhibited significantly different gene expression profiles as assessed by transcriptome sequencing.One of the highly expressed genes in CAAs is granulocyte colony-stimulating factor(G-CSF).Treatment with recombinant human G-CSF protein or stable expression of human G-CSF in triple-negative breast cancer(TNBC)cell lines enhanced epithelial–mesenchymal transition,migration,and invasion of cancer cells,by activating Stat3.Accordantly,targeting G-CSF/Stat3 signaling with G-CSF-neutralizing antibody,a chemical inhibitor,or siRNAs for Stat3 could all abrogate CAA-or G-CSF-induced migration and invasion of breast cancer cells.The pro-invasive genes MMP2 and MMP9 were identified as target genes of G-CSF in TNBC cells.Furthermore,in human breast cancer tissues,elevated G-CSF expression in adipocytes is well correlated with activated Stat3 signal in cancer cells.Together,our results suggest a novel strategy to intervene with invasive breast cancers by targeting CAA-derived G-CSF. | Li Liu Yudong Wu Cheng Zhang Chong Zhou Yining Li Yi Zeng Chunbo Zhang Rong Li Daya Luo Lieliang Wang Long Zhang Shuo Tu Huan Deng Shiwen Luo Ye-Guang Chen Xiangyang Xiong Xiaohua Yan | 2020 | Journal of Molecular Cell Biology2020，12，9： | 4 |
| 5 | Suppression of growth and migration by blocking the hedgehog signaling pathway in gastric cancer cells显示文摘 | Runwei Yan Xiang Peng Xiaogang Yuan Dengliang Huang Jiang Chen Quqin Lu Nonghua Lv Shiwen Luo | 2013 | Cellular Oncology2013，，5： | 1 |
| 6 | Suppression of growth and migration by blocking the hedgehog signaling pathway in gastric cancer cells显示文摘 | Runwei Yan Xiang Peng Xiaogang Yuan Dengliang Huang Jiang Chen Quqin Lu Nonghua Lv Shiwen Luo | 2013 | Cellular Oncology2013，，5： | 1 |
| 7 | Suppression of growth and migration by blocking the hedgehog signaling pathway in gastric cancer cells显示文摘 | Runwei Yan Xiang Peng Xiaogang Yuan Dengliang Huang Jiang Chen Quqin Lu Nonghua Lv Shiwen Luo | 2013 | Cellular Oncology2013，，5： | 1 |
| 8 | CAD APPROACH FOR PLASTIC MOULD PARTING DIRECTIONS OPTIMIZATION显示文摘CADAPPROACHFORPLASTICMOULDPARTINGDIRECTIONSOPTIMIZATION①HuangCanmingLuoShiwenLiangTianpeiDepartmentofMechanicalEngineering,Ho... | Huang, Canming Luo, Shiwen Liang, Tianpei | 1998 | Chinese Journal of Mechanical Engineering1998，11，1： | 1 |
| 9 | Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system显示文摘Achieving uniform optical resolution for a large tissue sample is a major challenge for deep imaging.For conventional tissue clearing methods,loss of resolution and quality in deep regions is inevitable due to limited transparency.Here we describe the Transparent Embedding Solvent System(TESOS)method,which combines tissue clearing,transparent embedding,sectioning and block-face imaging.We used TESOS to acquire volumetric images of uniform resolution for an adult mouse whole-body sample.The TESOS method is highly versatile and can be combined with different microscopy systems to achieve uniformly high resolution.With a light sheet microscope,we imaged the whole body of an adult mouse,including skin,at a uniform 0.8×0.8×3.5μm^(3) voxel resolution within 120 h.With a confocal microscope and a 40×/1.3 numerical aperture objective,we achieved a uniform sub-micron resolution in the whole sample to reveal a complete projection of individual nerve axons within the central or peripheral nervous system.Furthermore,TESOS allowed the first mesoscale connectome mapping of individual sensory neuron axons spanning 5 cm from adult mouse digits to the spinal cord at a uniform sub-micron resolution. | Yating Yi Youqi Li Shiwen Zhang Yi Men Yuhong Wang Dian Jing Jiayi Ding Qingjie Zhu Zexi Chen Xingjun Chen Jun-Liszt Li Yilong Wang Jun Wang Hanchuan Peng Li Zhang Wenjing Luo Jian Q.Feng Yongwen He Woo-Ping Ge Hu Zhao | 2024 | Cell Research2024，34，2： | 0 |
| 10 | Aberrant expression of the extracellular matrix component Biglycan regulated by Hedgehog signalling promotes colorectal cancer cell proliferation显示文摘Hedgehog(Hh)signalling plays essential roles in regulating embryonic development and contributes to tumour initiation,growth and progression in multiple cancers.The detailed mechanism by which Hh signalling participates in tumour growth warrants thorough study,although several downstream target genes have been identified.Herein,a set of novel targets of Hh signalling was identified in multiple types of tumour cells via RNA-Seq analysis.Among these targets,the expression regulation and oncogenic function of the extracellular matrix component biglycan(BGN)were investigated.Further investigation verified that Hh signalling activates the expression of BGN via the transcription factor Gli2,which directly binds to the promoter region of BGN.Functional assays revealed that BGN facilitates tumour cell growth and proliferation in colorectal cancer(CRC)cells,and xenograft assays confirmed that BGN also promotes tumour growth in vivo.Moreover,analysis of clinical CRC samples showed that both the protein and mRNA levels of BGN are increased in CRC tissues compared to those in adjacent tissues,and higher expression of BGN is correlated with poorer prognosis of CRC patients,further confirming the function of BGN in CRC.Taken together,aberrantly activated Hh signalling increases the expression of BGN,possibly regulates the extracellular matrix,and thereby promotes tumour growth in CRC. | Shaopeng Zeng Feifei Zhou Yiqing Wang Zhenyu Zhai Linlin Xu Hailong Wang Xinping Chen Shiwen Luo Minzhang Cheng | 2022 | Acta Biochimica et Biophysica Sinica2022，54，2： | 0 |
| 11 | Mediator complex components are frequent targets for genetic alterations in various types of human cancer显示文摘The Mediator co-activator complex is a highly conserved,multisubunit protein complex required for gene transcription by RNA polymerase Ⅱ(RNAPⅡ)in all eukaryotes(Allen and Taatjes,2015).This complex,which consists of at least 30 polypeptides,can be divided into four structurally distinct sub-modules including the head,middle,tail,and cyclin-dependent kinase 8(CDK8) | Libin Deng Cheng Zhang Duling Miao Xiaoli Tang Shiwen Luo Hong-Bo Xin Huidong Shi | 2017 | Journal of Genetics and Genomics2017，44，12： | 0 |
| 12 | Designing interstitial boron-doped tunnel-type vanadium dioxide cathode for enhancing zinc ion storage capability显示文摘Chemical doping is a powerful method to intrinsically tailor the electrochemical properties of electrode materials.Here,an interstitial boron-doped tunnel-type VO_(2)(B)is constructed via a facile hydrothermal method.Various analysis techniques demonstrate that boron resides in the interstitial site of VO_(2)(B)and such interstitial doping can boost the zinc storage kinetics and structural stability of VO_(2)(B)cathode during cycling.Interestingly,we found that the boron doping level has a saturation limit peculiarity as proved by the quantitative analysis.Notably,the 2 at.%boron-doped VO_(2)(B)shows enhanced zinc ion storage performance with a high storage capacity of 281.7 mAh g^(-1) at 0.1 A g^(-1),excellent rate performance of 142.2 mAh g^(-1) at 20 A g^(-1),and long cycle stability up to 1000 cycles with the capacity retention of 133.3 mAh g^(-1) at 5 A g^(-1).Additionally,the successful preparation of the boron-doped tunneltype α-MnO_(2) further indicates that the interstitial boron doping approach is a general strategy,which supplies a new chance to design other types of functional electrode materials for multivalence batteries. | Shiwen Wang Hang Zhang Kang Zhao Wenqing Liu Nairui Luo Jianan Zhao Shide Wu Junwei Ding Shaoming Fang Fangyi Cheng | 2023 | Carbon Energy2023，5，8： | 0 |
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