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| 1 | Rice metabolic regulatory network spanning the entire life cycle显示文摘As one of the most important crops in the world,rice(Oryza sativa)is a model plant for metabolome research.Although many studies have focused on the analysis of specific tissues,the changes in metab-olite abundance across the entire life cycle have not yet been determined.In this study,combining both tar-geted and nontargeted metabolite profiling methods,a total of 825 annotated metabolites were quantified in rice samples from different tissues covering the entire life cycle.The contents of metabolites in different tissues of rice were significantly different,with various metabolites accumulating in the plumule and radicle during seed germination.Combining these data with transcriptome data obtained from the same time period,we constructed the Rice Metabolic Regulation Network.The metabolites and co-expressed genes were further divided into 12 clusters according to their accumulation patterns,with members within each cluster displaying a uniform and clear pattern of abundance across development.Using this dataset,we established a comprehensive metabolic profile of the rice life cycle and used two independent strategies to identify novel transcription factors-namely the use of known regulatory genes as bait to screen for new networks underlying lignin metabolism and the unbiased identification of new glycerophospholipid metabolism regulators on the basis of tissue specificity.This study thus demonstrates how guilt-by-association analysis of metabolome and transcriptome data spanning the entire life cycle in cereal crops provides novel resources and tools to aid in understanding the mechanisms underlying important agro-nomic traits. | Chenkun Yang Shuangqian Shen Shen Zhou Yufei Li Yuyuan Mao Junjie Zhou Yuheng Shi Longxu An Qianqian Zhou Wenju Peng Yuanyuan Lyu Xuemei Liu Wei Chen Shouchuang Wang Lianghuan Qu Xianqing Liu Alisdair R.Fernie Jie Luo | 2022 | Molecular Plant2022，15，2： | 3 |
| 2 | Modulation of tumor microenvironment by metal-organic-framework-derived nanoenzyme for enhancing nucleus-targeted photodynamic therapy显示文摘Photodynamic therapy(PDT)is a promising strategy for tumor treatment.Still,its therapeutic efficacy is compromised by the unsatisfactory cytotoxicity to specific subcellular organelles and insidious tumor microenvironment properties like hypoxia and high glutathione levels.Here,we fabricated a novel nanoenzyme that derived from metal-organic framework(MOF)with intrinsic catalase-like activities to decompose H2O2 to O2 and simultaneous glutathione consumption for enhancing PDT efficacy.The obtained Mn3O4 nanoparticle shows a larger pore size and surface area compared to native MOF particles,which can be used to load high dose photosensitizer.When decorated with AS1411 aptamer and polyethylene glycol(PEG),the obtained Mn3O4-PEG@C&A particle exhibits excellent stability and cell nucleus targeting ability.Remarkably,Mn3O4-PEG@C&A particle inhibited the tumor growth in the mouse model with high efficacy without any biotoxicity.This is the first report that applied MOF-derived nanoparticle to nucleus-targeted PDT.It may provide a new approach for designing functional nanoenzyme to subcellular organelles-targeted tumor modulation. | Xuemei Zeng Shuangqian Yan Peng Chen Wei Du Bi-Feng Liu | 2020 | Nano Research2020，13，6： | 3 |
| 3 | OsRLCK160 contributes to flavonoid accumulation and UV-B tolerance by regulating OsbZIP48 in rice显示文摘Plants produce specialized metabolites to adapt to the ever-changing environments.Flavonoids are antioxidants essential for growth,development,and breeding with increased stress resistance in crops.However,the mechanism of the involvement of flavonoids in ultraviolet-B(UV-B)stress in rice(Oryza sativa)is largely unknown.In this study,we cloned and functionally identified a receptor-like kinase(OsRLCK160)and a bZIP transcription factor(OsbZIP48)positively regulating flavonoid accumulation through metabolite-based genome-wide association study of the flavonoid content in rice.Meanwhile,OsRLCK160 interacted with and phosphorylated OsbZIP48 to regulate the flavonoid accumulation and participate in UV-B tolerance in rice.Our study indicates the importance of applying OsRLCK160 and OsbZIP48 to advance the fundamental understanding of stable rice production and breed UV-B-tolerant rice varieties,which may contribute to breeding high-yield rice varieties. | Feng Zhang Jiacheng Huang Hao Guo Chenkun Yang Yufei Li Shuangqian Shen Chuansong Zhan Lianghuan Qu Xianqing Liu Shouchuang Wang Wei Chen Jie Luo | 2022 | Science China(Life Sciences)2022，65，7： | 2 |
| 4 | Stability of the rarefaction wave for a two-fluid plasma model with diffusion显示文摘We study the large-time asymptotics of solutions toward the weak rarefaction wave of the quasineutral Euler system for a two-fluid plasma model in the presence of diffusions of velocity and temperature under small perturbations of initial data and also under an extra assumption θ_i,+/θ_e,+=θ_i,-/θ_e,-≥m_i/2m_e,namely, the ratio of the thermal speeds of ions and electrons at both far fields is not less than one half. Meanwhile,we obtain the global existence of solutions based on energy method. | DUAN RenJun LIU ShuangQian YIN HaiYan ZHU ChangJiang | 2016 | Science China Mathematics2016，59，1： | 2 |
| 5 | Combating the Coronavirus Pandemic:Early Detection,Medical Treatment,and a Concerted Effort by the Global Community显示文摘The World Health Organization(WHO)has declared the outbreak of 2019 novel coronavirus,known as 2019-nCoV,a pandemic,as the coronavirus has now infected over 2.6 million people globally and caused more than 185,000 fatalities as of April 23,2020.Coronavirus disease 2019(COVID-19)causes a respiratory illness with symptoms such as dry cough,fever,sudden loss of smell,and,in more severe cases,difficulty breathing.To date,there is no specific vaccine or treatment proven effective against this viral disease.Early and accurate diagnosis of COVID-19 is thus critical to curbing its spread and improving health outcomes.Reverse transcription-polymerase chain reaction(RT-PCR)is commonly used to detect the presence of COVID-19.Other techniques,such as recombinase polymerase amplification(RPA),loopmediated isothermal amplification(LAMP),clustered regularly interspaced short palindromic repeats(CRISPR),and microfluidics,have allowed better disease diagnosis.Here,as part of the effort to expand screening capacity,we review advances and challenges in the rapid detection of COVID-19 by targeting nucleic acids,antigens,or antibodies.We also summarize potential treatments and vaccines against COVID-19 and discuss ongoing clinical trials of interventions to reduce viral progression. | Zichao Luo Melgious Jin Yan Ang Siew Yin Chan Zhigao Yi Yi Yiing Goh Shuangqian Yan Jun Tao Kai Liu Xiaosong Li Hongjie Zhang Wei Huang Xiaogang Liu | 2020 | Research2020，，1： | 1 |
| 6 | Function of hydroxycinnamoyl transferases for the biosynthesis of phenolamides in rice resistance to Magnaporthe oryzae显示文摘Phenolamide(PA)metabolites play important roles in the interaction between plants and pathogens.The putrescine hydroxycinnamoyl transferase genes Os PHT3 and Os PHT4 positively regulate rice cell death and resistance to Magnaporthe oryzae.The b ZIP transcription factor APIP5,a negative regulator of cell death and rice immunity,directly binds to the Os PHT4 promoter to regulate putrescine-derived PAs.Whether other hydroxycinnamoyl transferase(HT)genes also participate in APIP5-mediated immunity remains unclear.Surprisingly,we find that genes encoding agmatine hydroxycinnamoyl transferases Os AHT1 and Os AHT2,tryptamine hydroxycinnamoyl transferases Os TBT1 and Os TBT2,and tyramine hydroxycinnamoyl transferases Os THT1 and Os THT2,responsible for the biosynthesis of polyamine-derived PAs are all up-regulated in APIP5-RNAi transgenic plants compared with segregated wild-type rice.Furthermore,both Os AHT1/2 and Os TBT1/2 are induced during M.oryzae infection,showing expression patterns similar to those previously reported for Os THT1/2 and Os PHT3/4.Transgenic plants overexpressing either Os AHT2-GFP or Os TBT1-GFP show enhanced resistance against M.oryzae and accumulated more PA metabolites and lignin compared with wild-type plants.Interestingly,as demonstrated for Os PHT4,APIP5 directly binds to the promoters of Os AHT1/2,Os TBT1/2,and Os THT1/2,repressing their transcription.Together,these results indicate that the HT genes are common targets of APIP5 and that PAs play critical roles in rice immunity. | Hong Fang Fan Zhang Chongyang Zhang Dan Wang Shuangqian Shen Feng He Hui Tao Ruyi Wang Min Wang Debao Wang Xionglun Liu Jie Luo Guo-Liang Wang Yuese Ning | 2022 | Journal of Genetics and Genomics2022，49，8： | 1 |
| 7 | The non-cutoff Vlasov-Maxwell-Boltzmann system with weak angular singularity显示文摘We establish the global existence of small-amplitude solutions near a global Maxwellian to the Cauchy problem of the Vlasov-Maxwell-Boltzmann system for non-cutoff soft potentials with weak angular singularity. This extends the work of Duan et al.(2013), in which the case of strong angular singularity is considered, to the case of weak angular singularity. | Yingzhe Fan Yuanjie Lei Shuangqian Liu Huijiang Zhao | 2018 | Science China Mathematics2018，61，1： | 1 |
| 8 | The Vlasov-Poisson-Boltzmann System without Angular Cutoff显示文摘 | Renjun Duan Shuangqian Liu | 2013 | Communications in Mathematical Physics2013，，1： | 1 |
| 9 | Smoothing Effects for the Classical Solutions to the Landau-Fermi-Dirac Equation显示文摘The smoothness of the solutions to the full Landau equation for Fermi-Dirac particles is investigated.It is shown that the classical solutions near equilibrium to the Landau-Fermi-Dirac equation have a regularizing effects in all variables (time,space and velocity),that is,they become immediately smooth with respect to all variables. | Shuangqian LIU | 2012 | Chinese Annals of Mathematics,Series B2012，33，6： | 0 |
| 10 | Heat Transfer Problem for the Boltzmann Equation in a Channel with Diffusive Boundary Condition显示文摘In this paper,the authors study the 1 D steady Boltzmann flow in a channel.The walls of the channel are assumed to have vanishing velocity and given temperaturesθ0andθ1.This problem was studied by Esposito-Lebowitz-Marra(1994,1995)where they showed that the solution tends to a local Maxwellian with parameters satisfying the compressible Navier-Stokes equation with no-slip boundary condition.However,a lot of numerical experiments reveal that the fluid layer does not entirely stick to the boundary.In the regime where the Knudsen number is reasonably small,the slip phenomenon is significant near the boundary.Thus,they revisit this problem by taking into account the slip boundary conditions.Following the lines of[Coron,F.,Derivation of slip boundary conditions for the Navier-Stokes system from the Boltzmann equation,J.Stat.Phys.,54(3-4),1989,829-857],the authors will first give a formal asymptotic analysis to see that the flow governed by the Boltzmann equation is accurately approximated by a superposition of a steady CNS equation with a temperature jump condition and two Knudsen layers located at end points.Then they will establish a uniform L∞estimate on the remainder and derive the slip boundary condition for compressible Navier-Stokes equations rigorously. | Renjun DUAN Shuangqian LIU Tong YANG Zhu ZHANG | 2022 | Chinese Annals of Mathematics,Series B2022，43，6： | 0 |
| 11 | Elucidation of the melitidin biosynthesis pathway in pummelo显示文摘Specialized plant metabolism is a rich resource of compounds for drug discovery.The acylated flavonoid glycoside melitidin is being developed as an anti-cholesterol statin drug candidate,but its biosynthetic route in plants has not yet been fully characterized.Here,we describe the gene discovery and functional characterization of a new flavonoid gene cluster(UDP-glucuronosyltransferases(Cg UGTs),1,2rhamnosyltransferase(Cg1,2Rha T),acyltransferases(Cg ATs))that is responsible for melitidin biosynthesis in pummelo(Citrus grandis(L.)Osbeck).Population variation analysis indicated that the tailoring of acyltransferases,specific for bitter substrates,mainly determine the natural abundance of melitidin.Moreover,3-hydroxy-3-methylglutaryl-Co A reductase enzyme inhibition assays showed that the product from this metabolic gene cluster,melitidin,may be an effective anti-cholesterol statin drug candidate.Co-expression of these clustered genes in Nicotiana benthamiana resulted in the formation of melitidin,demonstrating the potential for metabolic engineering of melitidin in a heterologous plant system.This study establishes a biosynthetic pathway for melitidin,which provides genetic resources for the breeding and genetic improvement of pummelo aimed at fortifying the content of biologically active metabolites. | Shuangqian Shen Shouchuang Wang Chenkun Yang Chao Wang Qianqian Zhou Shen Zhou Ran Zhang Yufei Li Zixuan Wang Liupan Dai Wenjv Peng Yingchen Hao Hao Guo Guangping Cao Xianqing Liu Fan Yao Qiang Xu Alisdair RFernie Jie Luo | 2023 | Journal of Integrative Plant Biology2023，65，11： | 0 |
| 12 | Integration of rhythmic metabolome and transcriptome provides insights into the transmission of rhythmic fluctuations and temporal diversity of metabolism in rice显示文摘Various aspects of the organisms adapt to cyclically changing environmental conditions via transcriptional regulation.However,the role of rhythmicity in altering the global aspects of metabolism is poorly characterized.Here,we subjected four rice(Oryza sativa)varieties to a range of metabolic profiles and RNA-seq to investigate the temporal relationships of rhythm between transcription and metabolism.More than 40%of the rhythmic genes and a quarter of metabolites conservatively oscillated across four rice accessions.Compared with the metabolome,the transcriptome was more strongly regulated by rhythm;however,the rhythm of metabolites had an obvious opposite trend between day and night.Through association analysis,the time delay of rhythmic transmission from the transcript to the metabolite level was~4 h under long-day conditions,although the transmission was nearly synchronous for carbohydrate and nucleotide metabolism.The rhythmic accumulation of metabolites maintained highly coordinated temporal relationships in the metabolic network,whereas the correlation of some rhythmic metabolites,such as branched-chain amino acids(BCAAs),was significantly different intervariety.We further demonstrated that the cumulative diversity of BCAAs was due to the differential expression of branched-chain aminotransferase 2 at dawn.Our research reveals the flexible pattern of rice metabolic rhythm existing with conservation and diversity. | Junjie Zhou Chengyuan Liu Qiyu Chen Ling Liu Shuying Niu Ridong Chen Kang Li Yangyang Sun Yuheng Shi Chenkun Yang Shuangqian Shen Yufei Li Junwei Xing Honglun Yuan Xianqing Liu Chuanying Fang Alisdair R.Fernie Jie Luo | 2022 | Science China(Life Sciences)2022，65，9： | 0 |
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