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| 1 | BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity显示文摘基础编辑() ,包含 cytidine 脱氨基酶和催化地有缺点的 Cas9,广泛地被用来执行基础编辑。然而,狭窄的编辑窗口是限制它的实用程序。这里,我们开发了依照到由熔化 10 的 U (T) 范围(加) 的更大的 C 为招募 GCN4 肽拷贝到 nCas9 (D10A ) 的编程作为基础编辑命名的一种新编辑技术 scFv-APOBEC-UGI-GB1 到目标地点。新系统完成与一扇拓宽的窗户的基础编辑,导致一个增加的指向染色体的范围。有趣地,新系统很产出更少讨厌的 indels 和 non-C-to-T 变换。我们也通过站 codons (iSTOP ) 的正式就职越过整个染色体在基因混乱表明了它的潜在的使用。一起拿,加系统与编辑窗口和提高的忠实增加提供一个新编辑工具。 | Wen Jiang Songjie Feng Shisheng Huang Wenxia Yu Guanglei Li Guang Yang Yajing Liu Yu Zhang Lei Zhang Yu Hou Jia Chen Jieping Chen Xingxu Huang | 2018 | Cell Research2018，28，8： | 20 |
| 2 | A multi-agent based intelligent configuration method for aircraft fleet maintenance personnel显示文摘A multi-agent based fleet maintenance personnel configuration method is proposed to solve the mission oriented aircraft fleet maintenance personnel configuration problem. The maintenance process of an aircraft fleet is analyzed first. In the process each aircraft contains multiple parts, and different parts are repaired by personnel with different majors and levels. The factors and their relationship involved in the process of maintenance are analyzed and discussed. Then the whole maintenance process is described as a 3-layer multi-agent system(MAS) model. A communication and reasoning strategy among the agents is put forward. A fleet maintenance personnel configuration algorithm is proposed based on contract net protocol(CNP). Finally, a fleet of 10 aircraft is studied for verification purposes. A mission type with 3 waves of continuous dispatch is imaged. Compared with the traditional methods that can just provide configuration results, the proposed method can provide optimal maintenance strategies as well. | Feng Qiang Li Songjie Sun Bo | 2014 | Chinese Journal of Aeronautics2014，27，2： | 16 |
| 3 | Generation of isogenic single and multiplex gene knockout mice by base editing-induced STOP显示文摘Although CRISPR/Cas9 has been widely used to generate knockout mice, two major limitations remain:the founders usually carry a mixture of genotypes, and mosaicism harboring multiple genotypes.Therefore, it takes a long time to get homozygous mutants. Recently developed base editing(BE) system,which introduces C-to-T conversion without double strand DNA cleavage, has been used to introduce artificial stop codons(i-STOP) to prematurely terminate translation, providing a cleaner strategy for genome engineering. Using this strategy, we generated CD160 KO and VISTA/CD160 double KO mice by microinjection of a single sg RNA targeting CD160 and a mixture of sg RNAs targeting VISTA and CD160,respectively. The BE system induced STOP efficiently in mouse embryos and consequently in founder mice without detectable off-target. Most interestingly, the majority of the mutants harbor same genetic modifications, indicating we generated isogenic single and multiplex gene mutant mice by BE-induced STOP. We also obtained homozygous mutant mouse in F1 mice, demonstrating the accelerated strategy in generating animal models. | Guang Yang Tianyu Zhu Zongyang Lu Guanglei Li Hao Zhang Songjie Feng Yajing Liu Jianan Li Yu Zhang Jia Chen Xuejiang Guo Xingxu Huang | 2018 | Science Bulletin2018，63，17： | 2 |
| 4 | Hydrogen Selective NH<sub>2</sub>‐MIL‐53(Al) MOF Membranes with High Permeability显示文摘 | Feng Zhang Xiaoqin Zou Xue Gao Songjie Fan Fuxing Sun Hao Ren Guangshan Zhu | 2012 | Adv Funct Mater2012，，17： | 1 |
| 5 | Petrogenesis of Late Mesozoic Calc-Alkaline Lamprophyres from Sulu UHP Terrane, Eastern China: Implications to Paleo-Pacific Plate Subduction and Destruction of the North China Craton显示文摘Destruction of the North China Craton has caused extensive concern on its multiple potential mechanisms including thermal erosion,chemical erosion and delamination.It is widely accepted that thinning of | WANG Zhuocheng WANG Lu Timothy KUSKY DING Yue WANG Songjie DENG Hao FENG Peng | 2016 | Acta Geologica Sinica(English Edition)2016，90，S1： | 0 |
| 6 | Construction and optimization of a base editor based on the MS2 system显示文摘Background:Catalytic defect Cas9‐cytosine deaminase fusion is widely used in base editing.The Multiple copy numbers of the MS2 binding site(MBS)can recruit multiple MS2 coat proteins(MCPs),which are usually applied to amplify signals.Our study aimed to apply the MS2 signal amplification system to the base editing system in order to achieve simultaneous mutations of multiple bases at the target genome site.Methods:Multiple copy numbers of the MS2 were ligated to the 3′‐end of sgRNA,and MCP was fused to the 5′‐end of cytosine deaminases.The MS2 was recognized by MCP to recruit cytosine deaminase for base substitutions(C‐T)at the target site.Different Cas9 variants,different cytosine deaminases and different copy numbers of MS2 were tested in this system,and the different versions of base editors were compared by editing efficiency and window.Results:In this study,dCas9,nCas9(D10A)and nCas9(H840A)were used.Among these 3 Cas9 variants,dCas9 exhibited higher base mutation efficiency.Two cytosine deaminases were then applied and the efficiency of rAPOBEC1 deaminase was found to be higher than AID.We also increased the copy numbers of MS2 linked to sgRNA from 2 to 12.Disappointingly,the sgRNA‐12x MS2 did not improve the editing efficiency or increase the editing window.Conclusion:An optimal version of base editor based on the MS2 system,MS2‐BErAPOBEC1(sgRNA‐2x MS2,MCP‐rAPOBEC1 and dCas9),was obtained.This tool can simultaneously mutate multiple bases at the target site,providing a new approach for the study of genome functions. | Shuzhen Zhang Songjie Feng Wen Jiang Xingxu Huang Jieping Chen | 2019 | Animal Models and Experimental Medicine2019，2，3： | 0 |
| 7 | 哺乳动物成熟配子的染色体三维结构及其在早期胚胎发育中的重编程规律显示文摘文章简介染色体高级结构在调控基因表达中发挥非常重要的作用。此前对于哺乳动物胚胎发育过程中染色体三维结构动态变化了解有限。课题组使用优化后的少量细胞Hi-C方法建立小鼠配子和早期胚胎的染色体三维结构图谱。 | Yuwen Ke Yanan Xu Xuepeng Chen Songjie Feng Zhenbo Liu Yaoyu Sun Xuelong Yao Fangzhen Li Wei Zhu Lei Gao Haojie Chen Zhenhai Du Wei Xie Xiaocui Xu 黄行许 刘江 | 2018 | 科学新闻2018，0，4： | 0 |
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