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| 1 | Indoleamine 2,3-dioxygenase (IDO) is essential for dendritic cell activation and chemotactic responsiveness to chemokines显示文摘Indoleamine 2, 3-dioxygenase (IDO) is a rate-limiting enzyme for the tryptophan catabolism. In human and murine cells, IDO inhibits antigen-specific T cell proliferation in vitro and suppresses T cell responses to fetal alloantigens during murine pregnancy. In mice, IDO expression is an inducible feature of specific subsets of dendritic cells (DCs), and is important for T cell regulatory properties. However, the effect of IDO and tryptophan deprivation on DC func- tions remains unknown. We report here that when tryptophan utilization was prevented by a pharmacological inhibitor of IDO, 1-methyl tryptophan (1MT), DC activation induced by pathogenic stimulus lipopolysaccharide (LPS) or inflam- matory cytokine TNF-α was inhibited both phenotypically and functionally. Such an effect was less remarkable when DC was stimulated by a physiological stimulus, CD40 ligand. Tryptophan deprivation during DC activation also regu- lated the expression of CCR5 and CXCR4, as well as DC responsiveness to chemokines. These results suggest that tryptophan usage in the microenvironment is essential for DC maturation, and may also play a role in the regulation of DC migratory behaviors. | Shih Ling HWANG Nancy Pei-Yee CHUNG Jacqueline Kwai-Yi CHAN Chen-Lung Steve LIN | 2005 | Cell Research2005，15，3： | 11 |
| 2 | Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells显示文摘AIM: To development of an improved p38 MAPK inhibitor-based serum-free medium for embryoid body cardiomyocyte differentiation of human pluripotent stem cells. METHODS: Human embryonic stem cells (hESC) differentiated to cardiomyocytes (CM) using a p38 MAPK inhibitor (SB203580) based serum-free medium (SB media). Nutrient supplements known to increase cell viability were added to SB medium. The ability of these supplements to improve cardiomyogenesis was evaluated by measurements of cell viability, total cell count, and the expression of cardiac markers via flow cytometry. An improved medium containing Soy hydrolysate (HySoy) and bovine serum albumin (BSA) (SupSB media) was developed and tested on 2 additional cell lines (H1 and Siu-hiPSC). Characterization of the cardiomyocytes was done by immunohistochemistry, electrophysiology and quantitative real-time reverse transcriptionpolymerase chain reaction. RESULTS: hESC cell line, HES-3, differentiating in SB medium for 16 d resulted in a cardiomyocyte yield of 0.07 ± 0.03 CM/hESC. A new medium (SupSB media) was developed with the addition of HySoy and BSA to SB medium. This medium resulted in 2.6 fold increase in cardiomyocyte yield (0.21 ± 0.08 CM/hESC). The robustness of SupSB medium was further demonstrated using two additional pluripotent cell lines (H1, hESC and Siu1, hiPSC), showing a 15 and 9 fold increase in cardiomyocyte yield respectively. The age (passage number) of the pluripotent cells did not affect the cardiomyocyte yields. Embryoid body (EB) cardiomyocytes formed in SupSB medium expressed canonical cardiac markers (sarcomeric α-actinin, myosin heavy chain and troponin-T) and demonstrated all three major phenotypes: nodal-, atrial- and ventricular-like. Electrophysiological characteristics (maximum diastolic potentials and action potential durations) of cardiomyocytes derived from SB and SupSB media were similar. CONCLUSION: The nutrient supplementation (HySoy and BSA) leads to increase in cell viability, cell yield and cardiac marker expression during cardiomyocyte differentiation, translating to an overall increase in cardiomyocyte yield. | Sherwin Ting Marti Lecina Yau-Chi Chan Hung Fat Tse Shaul Reuveny Steve KW Oh | 2013 | World Journal of Stem Cells2013，5，3： | 4 |
| 3 | Hedgehog Controls Hepatic Stellate Cell Fate by Regulating Metabolism显示文摘 | Yuping Chen Steve S. Choi Gregory A. Michelotti Isaac S. Chan Marzena Swiderska-Syn Gamze F. Karaca Guanhua Xie Cynthia A. Moylan Francesca Garibaldi Richard Premont Hagir B. Suliman Claude A. Piantadosi Anna Mae Diehl | 2012 | Gastroenterology2012，，5： | 2 |
| 4 | Outcome and prognostic factors in 110 consecutive patients with primary uterine leiomyosarcoma:A Rare Cancer Network study显示文摘Objective: Primary uterine leiomyosarcomas(ULMS) are rare,and the optimal treatment is controversial.We aimed to assess the outcome and prognostic factors in a multicenter population of women treated for primary ULMS.Methods: We retrospectively collected data of 110 women treated in 19 institutions of the Rare Cancer Network(RCN).Inclusion criteria consisted of a pathology report confirming the diagnosis of ULMS,aged 18–80 years,complete International Federation of Gynecology and Obstetrics(FIGO) stage information,complete information on treatment,and a minimum follow-up of 6 months.Local control(LC) and locoregional control(LRC),overall survival(OS) and disease-free survival(DFS) rates were computed using the Kaplan-Meier method.Univariate analysis was implemented using the log rank test,and multivariate analysis using the Cox model.Results: All patients underwent surgery.Seventy-five patients(68%) received adjuvant radiotherapy(RT),including brachytherapy in 18(16%).Seventeen patients(15%) received adjuvant chemotherapy.Median follow-up was 58(range,6–240) months.Five-year OS and DFS rates were 50% and 34%,and LC and LRC rates were 88%and 72%,respectively.On multivariate analysis,independent favorable prognostic factors were younger age,FIGO stage I,small tumor size,previous uterine disease,and no vascular invasion for OS and DFS.FIGO stage was the only favorable factor influencing LRC.Adjuvant local or systemic treatments did not improve the outcomes.Eight patients treated with RT presented a grade 3 acute toxicity,and only one patient with grade 3 late toxicity.Conclusions: In this large population of primary ULMS patients,we found good results in terms of LC and LRC.Nevertheless,OS remains poor,mainly due to the occurrence of distant metastases.An early diagnosis seemed to improve the prognosis of the patients.Adjuvant local or systemic treatments,or more aggressive surgicalprocedures such as the Wertheim procedure,did not seem to impact the outcome. | Alessandra Franzetti Pellanda Berardino De Bari Elisabeth Deniaud-Alexandre MarcoKrengli Paul Van Houtte Antonella Riehetti Salvador Villa Hadassah Goldberg EwaSzutowiez-Zielifiska Michel Bolla Heidi Rutten Marc Van Eijkeren Philip Poortmans Guido Henke Yavuz Anaeak Steve Chan Christine Landmann Carine Kirkove LueianoScandolaro Jacques Bernier Rene-Olivier Mirimanof Mahmut Ozsahin | 2017 | Chinese Journal of Cancer Research2017，29，6： | 2 |
| 5 | Genome-wide High-Resolution Mapping and Functional Analysis of DNA Methylation in Arabidopsis显示文摘 | Xiaoyu Zhang Junshi Yazaki Ambika Sundaresan Shawn Cokus Simon W.-L. Chan Huaming Chen Ian R. Henderson Paul Shinn Matteo Pellegrini Steve E. Jacobsen Joseph R. Ecker | 2006 | Cell2006，，6： | 2 |
| 6 | Distribution, abundance and diversity of Gambierdiscus spp. from a ciguatera-endemic area in Marakei, Republic of Kiribati显示文摘 | Yixiao Xu Mindy L. Richlen Steve L. Morton Yim Ling Mak Leo Lai Chan Aranteiti Tekiau Donald M. Anderson | 2014 | Harmful Algae2014，，： | 1 |
| 7 | Prostate apoptosis response-4 mediates trophic factor withdrawal-induced apoptosis of hippocampal neurons显示文摘 | Sic L Chan Steven P Tammariello Steve Estus | 1999 | Journal of Neurochemistry1999，73，2： | 1 |
| 8 | Mirror,Mirror on the Wall…Are the Freer Countries More Pacific?显示文摘 | Steve | 1984 | Jonnud of Conflit Resolution1984，，28： | 1 |
| 9 | Major-power intervention and war initiation by the weak显示文摘 | Steve Chan | 2010 | International Politics2010，47，2： | 1 |
| 10 | Chinese Political Attitudes and Values in Comparative Context: Cautionary Remarks on Cultural Attributions显示文摘 | Steve Chan | 2008 | Journal of Chinese Political Science2008，，3： | 1 |
| 11 | Alcohol Activates the Hedgehog Pathway and Induces Related Procarcinogenic Processes in the Alcohol‐Preferring Rat Model of Hepatocarcinogenesis显示文摘 | Isaac S. Chan Cynthia D. Guy Mariana V. Machado Abigail Wank Vishnu Kadiyala Gregory Michelotti Steve Choi Marzena Swiderska‐Syn Gamze Karaca Thiago A. Pereira Michele T. Yip‐Schneider C. Max Schmidt Anna Mae Diehl | 2014 | Alcohol Clin Exp Res2014，，3： | 1 |
| 12 | Genome-wide High-Resolution Mapping and Functional Analysis of DNA Methylation in Arabidopsis显示文摘 | Xiaoyu Zhang Junshi Yazaki Ambika Sundaresan Shawn Cokus Simon W.-L. Chan Huaming Chen Ian R. Henderson Paul Shinn Matteo Pellegrini Steve E. Jacobsen Joseph R. Ecker | 2006 | Cell2006，，6： | 1 |
| 13 | Basel III:Is the cure worse than the disease显示文摘 | Bill Allen Ka Kei Chan Alistair Milne Steve Thomas | 2012 | International Review of Financial Analysis2012，，25： | 1 |
| 14 | Hold the Drones: Fostering the Development of Big Data Paradigms through Regulatory Frameworks显示文摘 | Robert Spousta Steve Chan | 2015 | 通讯和计算机(中英文版)2015，12，3： | 1 |
| 15 | Explaining Wars Fought by Established Demoeraeies: Do Institutional Con- straints Matter? 显示文摘 | David Leblang Steve Chan | 2003 | Political Research Quarterly2003，，4： | 1 |
| 16 | Evaluating nurses knowledge, attitude and competency after an education programme on suicide prevention显示文摘 | Sally Wai - chi Chan Wai - tong Chien Steve Tso | 2009 | Nurse Education Today2009，29，： | 1 |
| 17 | Public Opinion as a Con- straint against War:Democracies&Responses to Oper- ation Iraqi Freedom 显示文摘 | Steve Chan William Safran | 2006 | Foreign Policy Analysis2006，，22： | 1 |
| 18 | Basel Ⅲ:Is the Cure Worse than the Disease显示文摘 | Bill Allen Ka Kei Chan Alistair Milne Steve Thomas | | 0，，25： | 1 |
| 19 | A Systematic Approach to Pulmonary Valve Replacement in the Current Era显示文摘Background:Pulmonary valve replacement(PVR)can be accomplished via surgical,transcatheter,or hybrid approaches.There are inherent advantages to transcatheter PVR and hybrid PVR without cardiopulmonary bypass.We review the methods and results of a standardized institutional approach to PVR.Methods:Retrospective review of all PVR cases between February 2017 and February 2020.Hybrid PVR entailed off-pump RVOT plication with percutaneous transcatheter PVR.Results:Primary transcatheter PVR was attempted in 37,hybrid PVR was performed in 11,and on-pump surgical PVR was performed in 9.Median age at PVR was 27 years(6–65).Primary transcatheter PVR was successful in 35/37(2 converted to surgical).Standard surgical PVR was utilized for positive coronary compression testing(n=4),stent/valve system migration(n=2),or patient preference(n=3).In the hybrid group mean RVOT diameter was 34 mm(32–38).Median length of stay was 1 day for transcatheter PVR,5 for surgical,and 3 for hybrid(p=0.02).Median follow-up was 1.5 years.Re-interventions were one balloon valve dilation in a transcatheter PVR,and one valve dilation with subsequent transcatheter valve-in-valve PVR in the surgical cohort.One hybrid patient expired 11 months post procedure.Conclusions:A systematic approach to PVR utilizing all approaches in pre-defined order of preference leads to consistent outcomes in a wide variety of anatomic configurations.Transcatheter PVR may be accomplished in the majority of patients.When necessary,hybrid off-pump RVOT plication with transcatheter PVR avoids the need for cardiopulmonary bypass. | R.Allen Ligon Larry A.Latson Mark M.Ruzmetov Kak-Chen Chan Todd Roth Immanuel I.Turner Frank G.Scholl Steve Bibevski | 2021 | Congenital Heart Disease2021，16，3： | 0 |
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