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| 1 | Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis显示文摘 | Yao Chen Yuxuan Zheng Yun Gao Zhen Lin Suming Yang Tongtong Wang Qiu Wang Nannan Xie Rong Hua Mingxi Liu Jiahao Sha Michael D. Griswold Jinsong Li Fuchou Tang Ming-Han Tong | 2018 | Cell Research2018，28，9： | 26 |
| 2 | Screening oligonucleotide sequences for silver staining and D-galactose visual detection using RCA silver staining in a tube显示文摘Oligonucleotides 为强烈染色银的重复序列被屏蔽。一个聚类 AG 的嘌呤序列显示出强壮的染色,并且染色的密度能被破坏聚类 AG 的顺序的连续性损害。赞成污点的顺序然后在为它的产品察觉卷圆扩大(RCA ) 被采用。一个染色试管的方法为方便、视觉的 RCA 试金被开发。而且由介绍 GalR 进 RCA,没有任何设备, d 半乳糖被与肉眼染色试管的 RCA 检测。大约 10 公里 d 半乳糖能容易被识别,并且 d 半乳糖的察觉与几另外的 monosaccharides 的比较是特定的。 | Ziwei Chen Xuying Duan Hua Wei Suming Tang Chidong Xu Yanlei Li Yifu Guan Guojie Zhao | 2018 | Acta Biochimica et Biophysica Sinica2018，50，5： | 0 |
| 3 | Reprogramming of mouse neural stem cells to induced pluripotent stem cells using Oct4 combined with microRNA显示文摘microRNA is important for maintaining characteristics of embryonic stem cells,and microRNA302a(MiR-302a) has been shown to exert important effects on cell reprogramming.Therefore,the present study used miR294 and miR302a,in combination with Oct4,to induce mouse neural stem cells(NSCs) into induced pluripotent stem(iPS) cells.Following identification of iPS cells,the effects of microRNA on cell reprogramming were analyzed.Results suggested that reprogramming efficiency with Oct4 + miR-294 + miR-302a was 7-fold greater than Oct4 alone(0.1% vs.0.014%).The iPS cells were undifferentiated and positive for alkaline phosphatase,SSEA-1,and Oct4.These findings demonstrated that microRNAs play an important role in cell reprogramming and provide a safe and efficient induction system for cellular reprogramming. | Qiuyue Yan Jie Xu Yanqiang Zhan Zhouping Tang Suming Zhang | 2011 | Neural Regeneration Research2011，6，1： | 0 |
| 4 | Effects of cations on small fragment of DNA polymerase I using a novel FRET assay显示文摘DNA 聚合酶(PolI ) 我由朊酶消化了生产包含 53 exonuclease 活动的小碎片(SF ) 。polI 的 53exonuclease 活动在 vivo 在 DNA 复制期间劈开下游的 RNA 教材海滨。以前的在里面 vitro 研究建议了它劈开的能力从 5 终端和水晶另外的核酸酶和生物化学的数据组织的 flap-structure-specific endonuclease activity.From 双, two-metal-ion 机制被求婚了,但是让 notbeen 坚定。在这研究,我们克隆,表示,并且净化,并且建立了新奇荧光 resonanceenergy 转移 SF 蛋白质(烦恼) 分析 SF 蛋白质的催化活动的试金。itscatalytic 能力上的几个金属离子的效果用这被分析烦恼试金。结果证明 Mg 2+, Mn 2+, 和 Zn 2+ 能激活 SF 的劈开,当 Ca 2+, Ni 2+, 和公司 2+ 不对 SF 催化作用合适时。K +, Na +, 和 dNTP 的效果也是坚定的。 | Guojie Zhao Suming Tang Jun Li Tianyu Hu Yifu Guan | 2014 | Acta Biochimica et Biophysica Sinica2014，46，8： | 0 |
| 5 | Revealing an initiation inhibition of RCA and its application in nucleic acid detection显示文摘Rolling circle amplification is a widely used biosensing technique.Although various secondary structures have been employed in RCA,their effects on RCA efficiency have seldom been reported.Here,we find that stems in circular templates can strongly inhibit RCA,and the primer-stem distance is responsible for the inhibition.Based on the results,we propose an initiation inhibition mechanism and present a design principle for a general RCA assay.Inspired by this mechanism,we further propose a new nucleic acid detection method.The results verify that this method can increase RCA detection sensitivity according to the target recycling principle.Besides DNA detection,it can also achieve single mismatch discrimination of miRNA detection after optimization.This method also shows convenient visualization detection.The initiation inhibition of RCA could be helpful for RCA applications as promising detection techniques. | Qian Zhuang Meiqiong Ge Xiaodong Yu Jing Chai Lu Zhang Suming Tang Hua Wei Jinxin Zhang Guojie Zhao | 2023 | Acta Biochimica et Biophysica Sinica2023，55，4： | 0 |
| 6 | Electrocardiogram changes during hematoma enlargement in intracerebral hemorrhage patients显示文摘BACKGROUND:It has been reported that cerebrovascular disease causes changes in electrocardiogram results.OBJECTIVE:To investigate changes in electrocardiogram results in patients with intracerebral hematoma enlargement.DESIGN,TIME AND SETTING:The present case-retrospective analysis study was performed at the Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology from January 2005 to October 2006.PARTICIPANTS:A total of 225 intracerebral hemorrhage patients(142 males and 83 females) that were hospitalized at the Department of Neurology were enrolled the present study.The patient selection was in accordance with diagnostic criteria from the Fourth National Cerebrovascular Disease Congress of China in 1995,and diagnosis was confirmed using computed tomography.All patients underwent computed tomography twice within 24 hours following intracerebral hemorrhage,and were subjected to electrocardiogram examination after admission.METHODS:According to hematoma enlargement following intracerebral hemorrhage,all patients were divided into hematoma enlargement(n = 20) and non-hematoma enlargement(n = 205) groups.Because of the large patient number difference between the two groups,the hematoma enlargement group was matched with the non-hematoma enlargement group.Patients meeting these conditions were included in the non-hematoma enlargement group.Finally,75 patients were included in the final analysis,19 in the hematoma enlargement group and 56 in the non-hematoma enlargement group.Clinical data from the two groups were statistically analyzed.MAIN OUTCOME MEASURES:The incidence of electrocardiographic abnormalities between the hematoma enlargement and non-hematoma enlargement groups.RESULTS:In the hematoma enlargement group,15 patients(79%) developed electrocardiographic abnormalities.In the non-hematoma enlargement group,24 patients(43%) presented with electrocardiographic abnormalities.There were significant differences in electrocardiographic abnormalities between the groups(P < 0.01).CONCLUSION:Patients with electrocardiographic abnormalities suffered from hematoma enlargement following admission. | Zhouping Tang Di Chen Xingyong Chen Xiangwu Meng Xuewei Xie Xiang Luo Suming Zhang | 2008 | Neural Regeneration Research2008，3，9： | 0 |
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