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| 1 | Development and utilization of a new chemically-induced soybean library with a high mutation density显示文摘Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate(EMS)-induced soybean(Glycine max) population,consisting of 21,600 independent M_2 lines, was developed.Over 1,000 M_(4(5))families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll(Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene(ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean. | Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu Jinhui Lei Ying Liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu | 2017 | Journal of Integrative Plant Biology2017，59，1： | 6 |
| 2 | Effect of pH,temperature and heating time on the formation of furan in sugar–glycine model systems显示文摘Furan(C4H4O)has been classified as a possible animal and human carcinogen by many international agencies.The formation of furan in three sugar–glycine models using glucose,fructose,and sucrose was investigated using headspace gas chromatography mass spectrometry method(HSGC–MS)with various dual combinations of three important heat processing conditions,i.e.pH,temperature,and heating time.Results indicated that furan levels from sugar–glycine model systems during the thermal processing can be attributed to selective sugar types,pH,temperature,and heating time.In glucose–glycine and fructose–glycine system,the lowest furan level was detected in acid condition but in sucrose–glycine system furan formed significantly lower(P<0.05)in acidic conditions the lowest furan level was found in alkaline conditions.The furan levels were observed to increase with heating time in all three model systems.Furthermore,less furan was generated in non-reducing sugar system(sucrose)than in reducing sugar system(glucose and fructose).Therefore,they demonstrate the possibility of limiting the formation of furan in heat processed foods by both the careful selection of carbohydrates(i.e.non-reducing sugars and reducing sugars)ingredients and appropriate processing conditions.©2013 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved. | Shaoping Nie Jungen Huang Jielun Hu Yanan Zhang Sunan Wang Chang Li Massimo Marcone Mingyong Xie | 2013 | Food Science and Human Wellness2013，2，2： | 4 |
| 3 | Interference-Driven Designs of Nonlinear-Phase FIR Filter with Application in FBMC System显示文摘In the forthcoming 5G systems, new technologies, such as amorphous networks and non-orthogonal filter bank multicarriers(FBMC), provide an effective way to accommodate high-rate transmissions. Meanwhile, the prototype filter affects the adjacent channel interference, and therefore is important for FBMC systems. Besides, once the amorphous network is taken into account, the requirement for interference controlmust be much stricter. Accordingly, this paper focuses on the design of prototype filter with better ability of interference controlling, where we exploit the nonlinear phase FIR filter(NLPFF) instead of traditional linear phase FIR filter(LPFF) to achieve more optimization spaces under a small sacrifice of linear phase. In ourdesigns, both the amplitude and phase responsesare handled independently to approach the stopband performance enhancements, in while the nearly perfect reconstruction(NPR) conditionsare relaxed by pre-specified thresholds. Computer simulations confirm the effectiveness of the NLPFF designs, and demonstrate the advantages of the proposed NLPFF in FBMC applications. | Jiangang Wen Jingyu Hua Sunan Li Kai Zhou Dongming Wang | 2016 | China Communications2016，13，12： | 4 |
| 4 | Identification of a novel seed size associated locus SW9-1 in soybean显示文摘Seed size is one of the vital traits determining seed appearance, quality, and yield. Untangling the genetic mechanisms regulating soybean 100-seed weight (100-SW), seed length and seed width across environments may provide a theoretical basis for improving seed yield. However, there are few reports related to QTL mapping of 100-SW across multiple ecological regions. In this study, 21 loci associated with seed size traits were identified using a genome-wide association of 5361 single nucleotide polymorphisms (SNPs) across three ecoregions in China, which could explain 8.12%–14.25% of the phenotypic variance respectively. A new locus, named as SW9-1 on chromosome 9 that explained 10.05%–10.93% of the seed weight variance was found significantly related to seed size traits, and was not previously reported. The selection effect analysis showed that SW9-1 locus has a relatively high phenotypic effect (13.67) on 100-SW, with a greater contribution by the accessions with bigger seeds (3.69) than the accessions with small seeds (1.66). Increases in seed weight were accompanied by increases in the frequency of SW9-1T allele, with >90% of the bred varieties with a 100-SW >30 g carrying SW9-1T. Analysis of SW9-1 allelic variation in additional soybean accessions showed that SW9-1T allele accounting for 13.83% of the wild accessions, while in 46.55% and 51.57% of the landraces and bred accessions, respectively, this results indicating that the SW9-1 locus has been subjected to artificial selection during the early stages of soybean breeding, especially the utilization of SW9-1T in edamame for big seed. These results suggest that SW9-1 is a novel and reliable locus associated with seed size traits, and might have an important implication for increasing soybean seed weight in molecular design breeding. Cloning this locus in future may provide new insights into the genetic mechanisms underlying soybean seed size traits. | Jiajia Li Jinghui Zhao Yinghui Li Yali Gao Sunan Hua Muhammad Nadeem Genlou Sun Wenming Zhang Jinfeng Hou Xiaobo Wang Lijuan Qiu | 2019 | The Crop Journal2019，7，4： | 4 |
| 5 | Comparative population genomics dissects the genetic basis of seven domestication traits in jujube显示文摘Jujube(Ziziphus jujuba Mill.)is an important perennial fruit tree with a range of interesting horticultural traits.It was domesticated from wild jujube(Ziziphus acidojujuba),but the genomic variation dynamics and genetic changes underlying its horticultural traits during domestication are poorly understood.Here,we report a comprehensive genome variation map based on the resequencing of 350 accessions,including wild,semi-wild and cultivated jujube plants,at a>15×depth.Using the combination of a genome-wide association study(GWAS)and selective sweep analysis,we identified several candidate genes potentially involved in regulating seven domestication traits in jujube.For fruit shape and kernel shape,we integrated the GWAS approach with transcriptome profiling data,expression analysis and the transgenic validation of a candidate gene to identify a causal gene,ZjFS3,which encodes an ethyleneresponsive transcription factor.Similarly,we identified a candidate gene for bearing-shoot length and the number of leaves per bearing shoot and two candidate genes for the seed-setting rate using GWAS.In the selective sweep analysis,we also discovered several putative genes for the presence of prickles on bearing shoots and the postharvest shelf life of fleshy fruits.This study outlines the genetic basis of jujube domestication and evolution and provides a rich genomic resource for mining other horticulturally important genes in jujube. | Mingxin Guo Zhongren Zhang Yanwei Cheng Sunan Li Peiyin Shao Qiang Yu Junjie Wang Gan Xu Xiaotian Zhang Jiajia Liu Linlin Hou Hanxiao Liu Xusheng Zhao | 2020 | Horticulture Research2020，7，1： | 2 |
| 6 | Remote plasma-enhanced atomic layer deposition of gallium oxide thin films with NH_3 plasma pretreatment显示文摘High quality gallium oxide(Ga_2O_3) thin films are deposited by remote plasma-enhanced atomic layer deposition(RPEALD) with trimethylgallium(TMG) and oxygen plasma as precursors. By introducing in-situ NH3 plasma pretreatment on the substrates, the deposition rate of Ga_2O_3 films on Si and GaN are remarkably enhanced, reached to 0.53 and 0.46 ?/cycle at 250 °C,respectively. The increasing of deposition rate is attributed to more hydroxyls(–OH) generated on the substrate surfaces after NH3 pretreatment, which has no effect on the stoichiometry and surface morphology of the oxide films, but only modifies the surface states of substrates by enhancing reactive site density. Ga_2O_3 film deposited on GaN wafer is crystallized at 250 °C, with an epitaxial interface between Ga_2O_3 and GaN clearly observed. This is potentially very important for reducing the interface state density through high quality passivation. | Hui Hao Xiao Chen Zhengcheng Li Yang Shen Hu Wang Yanfei Zhao Rong Huang Tong Liu Jian Liang Yuxin An Qing Peng Sunan Ding | 2019 | Journal of Semiconductors2019，40，1： | 1 |
| 7 | Pathological characteristics and immunophenotype analysis of cervical intraepithelial neoplasia显示文摘Objective To explore the clinical pathological features and immunophenotypes of cervical intraepithelial neoplasia(CIN).Methods The protein expression of p16, p53, Bcl-2, and c-erb B-2 in 59 cases of CIN, 20 cases of cervical squamous cell carcinoma, and 20 cases of normal cervical tissues were tested using immunohistochemistry staining.Results The expression rates of p16, p53, Bcl-2, and c-erb B-2 in CIN tissues were 76.3%(45/59), 28.85(17/59), 61.0%(36/59), and 40.0%(23/59), respectively. The expression rates of p16, p53, Bcl-2, and c-erb B-2 in cervical squamous cell carcinoma tissues were 60.0%(12/20), 60.0%(12/20), 75.0%(15/20), and 65.0%(13/20), respectively. The expression rates of p16, p53, Bcl-2, and c-erb B-2 in normal cervical tissues were 0.0%(0/20), 0.0%(0/20), 35.0%(7/20), 0.0%(0/20), respectively. In comparison to normal tissues, the differential expressions of p16, p53, and Bcl-2 in squamous cell carcinoma and CIN were statistically significant(P <0.001), whereas the difference between the squamous cell carcinoma and CIN was not significant(P >0.05). In comparison to normal tissues, the differential expressions of c-erb B-2 and p53 in squamous cell carcinoma and CIN were statistically significant(P >0.05). The differential expressions of Bcl-2, c-erb B-2, and p53 in CIN 3 were statistically significant in comparison to CIN 1 and CIN 2(P <0.05). Conclusion Overexpression of Bcl-2 occurs early in the development of cervical cancer, whereas p16 and c-erb B-2 overexpression are markers for cell malignancy. The expression of p53 is correlated with the development of cervical cancer. | Yingying Li Sunan Wang Yangkun Wang Xingzhen Zeng | 2016 | Oncology and Translational Medicine2016，2，6： | 0 |
| 8 | 胃癌组织中HER2基因扩增与蛋白表达的研究(英文)显示文摘Objective: The aim of the study was to investigate the human epidermal growth factor receptor 2(HER2) gene amplification and protein expression and interpretation points in the stomach mixed carcinomas. Methods: Immunohistochemistry(IHC) and fluorescence in situ hybridization(FISH) technique were used to detect HER2 gene amplification and expression of HER2 protein in 442 cases of gastric mixed carcinoma. Results: The expression rate of HER2 protein was 41.2%(182/442): the HER2 protein expression IHC 3+ extensive type in 18 cases, partial type in 21 cases, focal type in 8 cases, accounting for 10.6%(47/442); the HER2 protein expression IHC 2+ extensive type in 23 cases, partial type in 28 cases, focal type in 11 cases, accounting for 14.0%(62/442); the HER2 protein expression IHC 1+ extensive type in 27 cases, partial type in 31 cases, focal type in 15 cases, accounting for 16.5%(73/442). HER2 gene amplification rate of 442 cases was 16.1%(71/442). In 182 cases of HER2 protein positive expression, the HER2 gene cluster amplification rate was 14.8%(27/182), large granular amplification rate 11.0%(20/182), punctate amplification rate 6.0%(11/182) and high polysomy 7.1%(13/182). In 71 cases of HER2 gene amplification, there was 42 cases of HER2 protein expression IHC 3+, 22 cases of HER2 protein expression IHC 2+, and 7 cases of IHC 1+. Conclusion: HER2 detection of gastric mixed carcinoma has great heterogeneity, HER2 protein positive expression is divided into extensive type, partial type and focal type, and HER2 gene positive amplification is divided into cluster amplification, large granular amplification, punctate amplification and high polysomy. These typing of HER2 protein expression and HER2 gene amplification provide reference index to quantify for targeted therapeutic effect of anticancer drugs. | Sunan Wang Yingying Li Zhengshun Xu Wenzhao Zhao Tian Yun Wuling Zhu Yangkun Wang | 2014 | The Chinese-German Journal of Clinical Oncology2014，13，6： | 0 |
| 9 | Synergistic effect of solvent and solid additives on morphology optimization for high-performance organic solar cells显示文摘Controlling the photoactive layer morphology towards nanoscale bi-continuous donor/acceptor interpenetrating networks is a key issue to build high-performance organic solar cells(OSCs).Due to the distinct properties between donor and acceptor materials,casting an active layer from a single solvent solution usually results in either insufficient or excessive phase separation that reduces the device performance.In comparison to the fullerene acceptors with closed-cage structures,the currently dominant non-fullerene acceptors possess the similar anisotropicπ-πinteractions with p-type organic semiconductor donors,giving rise to the complexity of the morphology regulation.Herein,we employ 4,4′-dimethoxyoctafluorobiphenyl(OFP)with strong crystallinity as a volatile solid additive to optimize the active layer morphology of OSCs.The synergistic effect of 1-chloronaphthalene(CN)and OFP as dual additives shows supreme capability on optimizing the morphology over the conventional additive of CN,which is in favor of improving charge transport and suppressing charge recombination for higher fill factors in various systems.In particular,the PTQ10:m-BTP-C6 Ph-based device processed by the additive showed a remarkable powerconversion efficiency(PCE)of 17.74%,whereas the control device processed by CN additive yielded a relatively lower PCE of16.45%. | Chenling Fan Hang Yang Qing Zhang Sunan Bao Hongyu Fan Xianming Zhu Chaohua Cui Yongfang Li | 2021 | Science China Chemistry2021，64，11： | 0 |
| 10 | Epitaxial growth ofβ-Ga_(2)O_(3)thin films on Ga_(2)O_(3)and Al_(2)O_(3)substrates by using pulsed laser deposition显示文摘In this work,we have successfully grown high quality epitaxialβ-Ga_(2)O_(3)thin films onβ-Ga_(2)O_(3)(100)and Al_(2)O_(3)(0001)substrates using pulsed laser deposition(PLD).By optimizing temperature and oxygen pressure,the best conditions were found to be 650-700℃and 0.5 Pa.To further improve the quality of hetero-epitaxialβ-Ga_(2)O_(3),the sapphire substrates were pretreated for atomic terraced surface by chemical cleaning and high temperature annealing.From the optical transmittance measurements,the films grown at 600-750℃exhibit a clear absorption edge at deep ultraviolet region around 250-275 nm wavelength.High resolution transmission electron microscope(HRTEM)images and X-ray diffraction(XRD)patterns demonstrate thatβ-Ga_(2)O_(3)(-201)//Al_(2)O_(3)(0001)epitaxial texture dominated the epitaxial oxide films on sapphire substrate,which opens up the possibilities of high power electric devices. | Yuxin An Liyan Dai Ying Wu Biao Wu Yanfei Zhao Tong Liu Hui Hao Zhengcheng Li Gang Niu Jinping Zhang Zhiyong Quan Sunan Dingy | 2019 | Journal of Advanced Dielectrics2019，9，4： | 0 |
| 11 | Multi-temporal urban semantic understanding based on GF-2 remote sensing imagery:from tri-temporal datasets to multi-task mapping显示文摘High resolution satellite images are becoming increasingly available for urban multi-temporal semantic understanding.However,few datasets can be used for land-use/land-cover(LULC)classification,binary change detection(BCD)and semantic change detection(SCD)simultaneously because classification datasets always have one time phase and BCD datasets focus only on the changed location,ignoring the changed classes.Public SCD datasets are rare but much needed.To solve the above problems,a tri-temporal SCD dataset made up of Gaofen-2(GF-2)remote sensing imagery(with 11 LULC classes and 60 change directions)was built in this study,namely,the Wuhan Urban Semantic Understanding(WUSU)dataset.Popular deep learning based methods for LULC classification,BCD and SCD are tested to verify the reliability of WUSU.A Siamese-based multi-task joint framework with a multi-task joint loss(MJ loss)named ChangeMJ is proposed to restore the object boundaries and obtains the best results in LULC classification,BCD and SCD,compared to the state-of-the-art(SOTA)methods.Finally,a large spatial-scale mapping for Wuhan central urban area is carried out to verify that the WUsU dataset and the ChangeMJ framework have good application values. | Sunan Shi Yanfei Zhong Yinhe Liu Jue Wang Yuting Wan Ji Zhao Pengyuan Lv Liangpei Zhang Deren Li | 2023 | International Journal of Digital Earth2023，16，1： | 0 |
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