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| 1 | Molecular cloning of human heat shock protein 27 and study of its protective effects on oxidative damage in rat cardiomyocte H9c2显示文摘Objective: To clone human cardiac heat shock protein 27(HSP27) gene and to determine the effects of HSP27 on the oxidative stress in rat cardiomyocyte cell line H9c2. Methods: Full length of HSP27 cDNA which got by RT-PCR was constructed into pCDNA3.1+. The recombinant was transfected into rat cardiomyocyte cell line H9c2 and the stable transfection cell line was selected by G418. Then we observe the effects of HSP27 over-expression on LDH release and apoptosis induced H2O2 in H9c2. Results:①pCDNA3.1+/HSP27 provided a sound expression of HSP27 in both 293T and H9c2. ②LDH releasing induced by 0, 100,250,500,1000 μmol/L H2O2 in HSP27 over-expression group and wild type group were 0.396±0.017 vs. 0.390±0.009 (p>0.05), 0.437±0.014 vs. 0.416±0.015 (P<0.05), 0.471±0.018 vs. 0.417±0.009 (P<0.001), 0.505±0.030 vs. 0.657±0.022(P<0.001),0.547±0.027 and 0.661±0.011(P<0.001),respectively. ③Apoptosis induced by 150 μmol/L H2O2 in HSP27 over-expression group and wild type group were (10.693±1.122)% vs. (4.027±1.628)%(P<0.01). Conclusion: We cloned and constructed human cardiac HSP27 gene successfully, and over-expression of human HSP27 could inhibit oxidative damage significantly in H9c2. | Li Liu Xiaojin Zhang Surong Jiang Xiang Gao Guoxain Ding Yunlin Cheng | 2005 | Journal of Nanjing Medical University2005，19，4： | 3 |
| 2 | Determination of trace bisphenol A in complex samples using selectivemolecularly imprinted solid-phase extraction coupled with capillary electrophoresis显示文摘 | Surong Mei Da Wub Ming Jiang | 2011 | Microchemical Journal2011，98，： | 1 |
| 3 | Determination of trace bisphenol A in complex samples using selective molecularly imprinted solid-phase extraction coupled with capillary electrophoresis显示文摘 | MEI Surong WU Da JIANG Ming | 2011 | Microchemical Journal2011，98，1： | 1 |
| 4 | Determination of trace bisphenol A in complex samples using selective molecularly imprinted solid-phase extraction coupled with capillary electrophoresis 显示文摘 | Mei Surong Wu Da Jiang Ming | 2011 | Microehemical Journal2011，98，1： | 1 |
| 5 | Re-search on the Kinematic Properties of a Sperm -like Swimming Micro Robot显示文摘 | Chen Bai Jiang Surong Liu Yaodong | 2010 | Journal of BionicEngineering2010，7，： | 1 |
| 6 | A CONTROLLED STUDY OF THERAPEUTIC EFFECTS OF COMPUTER-CONTROLLED ELECTRIC ACUPUNCTURE TREATMENT ON REFRACTORY SCHIZOPHRENIA显示文摘Out of 100 cases of intractable schizophrenia,57 cases were in the group ofComputer-Controlled Electric Acupuncture (CCEA) and 43 in the control group.The therapeutic re-sults showed that clinically CCEA had a remarkably effective rate of 40. 35% on refractoryschizophrenia,which had much better curative effects than those in the control group.Through dy-namic evaluation of brief psychiatric rating scale (BPRS),negative symptom scale (SANS) and posi-tive symptom scale (SAPS) for both groups before,during and after treatment,the results indicatedthat in CCEA group the scores of BPRS,SANS and SAPS after treatment were much less than thosebefore treatment,which were remarkably different from those in the control group (P<0.01).Lab-oratory examination of thyroxin and androgen of patients in both groups before and after treatmentfound a significant result. | Zhang Ben Meng Surong Yu Jiang Quan Changgeng Li Wentong Sun Hexiang Zhang Baoting Shi Qiuying Kailuan Psychiatric Hospital,Tangshan 063001,ChinaWang Jianjun Kailuan Hospital,Tangshan 063001,China | 1993 | World Journal of Acupuncture-Moxibustion1993，3，4： | 1 |
| 7 | α-Lipoic acid protected cardiomyoblasts from the injury induced by sodium nitroprusside through ROS-mediated Akt/Gsk-3β activation显示文摘 | Surong Jiang Weina Zhu Jun Wu Chuanfu Li Xiaojin Zhang Yuehua Li Kejiang Cao Li Liu | 2014 | Toxicology in Vitro2014，，8： | 1 |
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