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| 1 | Discovery of Late Paleozoic retrograded eclogites from the middle part of the northern margin of North China Craton显示文摘The retrograded eclogites have been discovered in the middle part of the northern margin of the North China Craton, which occur as lens or boudin within bio-tite-plagioclase gneisses in Paleoproterozoic Hongqiyingzi Group. The peak eclogite facies (P > 1.40—1.50 GPa, T = 680—730℃) mineral assemblage is composed of garnet, ompha-cite and rutile (±quartz), which was overprinted by the granulite facies mineral assemblage of vermicular symplec-tite of sodic clinopyroxene and plagioclase which replaced the precursory omphacite, and then amphibolite facies ret-rograded minerals with characterization of Amp+Pl ke-lyphitic rim and symplectite, and amphibole replaced clino-pyroxene. The protolith of retrograded eclogites is oceanic basalt formed at 438±11 Ma.The peak eclogite facies meta-morphic age of the retrograded eclogite is 325±4 Ma. These relict eclogites may be formed by the subduction of Pa-leo-Asian oceanic crust beneath the North China Craton during Late Paleozoic. The discovery of relict eclogite in this paper provides a new insight into farther understanding of tectonic evolution of the northern margin of the North China Craton, and the relationship between the Paleo-Asian Ocean and the North China Craton. | NIZhiyao ZHAIMingguo WANGRenmin TONGYing SHUGuiming HAIXiuling | 2004 | Chinese Science Bulletin2004，49，6： | 20 |
| 2 | In vitro and in vivo activity of D-serine in combination with β-lactam antibiotics against methicillin-resistant Staphylococcus aureus显示文摘As D-amino acids play important roles in the physiological metabolism of bacteria,combination of D-amino acids with antibiotics may provide synergistic antibacterial activity. The aim of the study was to evaluate in vitro and in vivo activity of D-serine alone and in combination withβ-lactams against methicillin-resistant Staphylococcus aureus(MRSA) strains, and to explore the possible sensitization mechanisms. The activity of D-serine, β-lactams alone and in combinations was evaluated both in vitro by standard MICs, time–kill curves and checkerboard assays, and in vivo by murine systemic infection model as well as neutropenic thigh infection model. An in vitro synergistic effect was demonstrated with the combination of D-serine and β-lactams against MRSA standard and clinical strains.Importantly, the combinations enhanced the therapeutic efficacy in the animal models as compared toβ-lactam alone groups. Initial mechanism study suggested possible revision of D-alanine-D-alanine residue to D-alanine-D-serine in peptidoglycan by adding of D-alanine in the medium, which may cause decreased affinity to PBPs during transpeptidation. In conclusion, D-serine had synergistic activity in combination with β-lactams against MRSA strains both in vitro and in vivo. Considering the relatively good safety of D-serine alone or in combination with β-lactams, D-serine is worth following up as new anti-MRSA infection strategies. | Qing Wang Yuemeng Lv Jing Pang Xue Li Xi Lu Xiukun Wang Xinxin Hu Tongying Nie Xinyi Yang Yan Q.Xiong Jiandong Jiang Congran Li Xuefu You | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 6 |
| 3 | Upregulation of miR-133a by adiponectin inhibits pyroptosis pathway and rescues acute aortic dissection显示文摘Acute aortic dissection(AAD)is a cardiovascular emergency caused by the formation of hematoma in the middle layer of the aortic wall.Adiponectin(APN)is an adipose tissue-specific protein that has anti-inflammation and anti-atherosclerosis functions.Pyroptosis,as an inflammatory cell death,depends on the activation of caspasel,while nucleotide-binding oligomerization domainlike receptor protein 3(NLRP3)is a typical representative of the pyroptosis pathway.In this study,we aimed to find whether APN affects the AAD process.The results showed that APN overexpression(OE)inhibited the AAD development and the levels of glucose,triglyceride,and total cholesteroI in mice model.In addition,APN OE inhibited the productions of gasdermin D(GSDMD),NLRP3,caspasel,interleukin-1β(IL-1β),IL-18,and osteopontin(OPN),as well as asmooth muscle actin(α-SMA)downregulation in vitro and in vivo.In addition,NLRP3 was found to be a target gene of miR-133a and miR-133a OE showed similar effects to APN OE in attenuating the LPS-induced productions of GSDMD,NLRP3,caspasel,IL-1βIL-18,and OPN,as well asα-SMA downregulation in vascular smooth muscle cells(vSMCs).Moreover,the beneficial effects of APN OE were abolished by miR-133a knockdown in vSMCs.In conclusion,our present results indicated that the upregulation of miR-133a by APN inhibits pyroptosis pathway,which potentially rescues AAD. | Haizhen Duan Xiaojun Zhang Renjie Song Tongying Liu Yuanyuan Zhang Anyong Yu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，9： | 4 |
| 4 | Meclofenamic acid represses spermatogonial proliferation through modulating m^6A RNA modification显示文摘Background: N6-Methyladenosine(m^6A), the most prevalent modification in mammalian m RNA, plays important roles in numerous biological processes. Several m^6A associated proteins such as methyltransferase like 3(METTL3),methyltransferase like 14(METTL14), α-ketoglutarate-dependent dioxygenase Alk B homolog 5(ALKBH5) and YTH domain containing 2(YTHDC2) are involved in the regulation of spermatogenesis and oogenesis. However, the role of the first detected m^6A demethylase, fat mass and obesity associate protein(FTO), in germ cells remains elusive.Elucidation of FTO roles in the regulation of germ cell fate will provide novel insights into the mammalian reproduction.Methods: Mouse GC-1 spg cells were treated with the ester form of meclofenamic acid(MA2) to inhibit the demethylase activity of FTO. The cellular m^6A and m^6Amlevel were analyzed through high performance liquid chromatography combined with tandem mass spectrometry(HPLC/MS-MS). The cell apoptosis was detected via TUNEL and flow cytometry. The cell proliferation was detected through Ed U and western blot. The m RNA level of core cyclin dependent kinases(CDKs) was quantified via q-PCR. RNA decay assay were performed to detect RNA stability. Dual fluorescence assay was conducted to study whether MA2 affects the expression of CDK2 dependent on the m^6A modification at 3’UTR.Results: MA2 significantly increased the cellular m^6A level and down-regulated the expression of CDK1, CDK2, CDK6 and Cd C25 a, resulting in arrest of G1/S transition and decrease of cell proliferation. MA2 downregulated CDK2 m RNA stability. Additionally, mutation of the predicted m^6A sites in the Cdk2–3’UTR could mitigated the degradation of CDK2 m RNA after MA2 treatment.Conclusion: MA2 affected CDKs expression through the m^6A-dependent m RNA degradation pathway, and thus repressed spermatogonial proliferation. | Tao Huang Jiayin Guo Yinghua Lv Yi Zheng Tongying Feng Qiang Gao Wenxian Zeng | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 4 |
| 5 | Genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii from Beijing,China显示文摘The objective of this study was to investigate the genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii clinical isolates from Beijing,China.173 A.baumannii clinical isolates from hospitals in Beijing from 2006 to 2009 were first subjected to high level aminoglycoside resistance(HLAR,MIC to gentamicin and amikacin>512 mg/mL)phenotype selection by broth microdilution method.The strains were then subjected to genetic basis analysis by PCR detection of the aminoglycoside modifying enzyme genes(aac(3)-Ⅰ,aac(3)-Ⅱc,aac(60)-Ⅰb,aac(60)-Ⅱ,aph(4)-Ⅰa,aph(30)-Ⅰ,aph(30)-Ⅱb,aph(30)-Ⅲa,aph(30)-Ⅵa,aph(2″)-Ⅰb,aph(2″)-Ⅰc,aph(2″)-Ⅰd,ant(2″)-Ⅰa,ant(3″)-Ⅰand ant(40)-Ⅰa)and the 16S rRNA methylase genes(armA,rmtB and rmtC).Correlation analysis between the presence of aminoglycoside resistance gene and HLAR phenotype were performed by SPSS.Totally 102(58.96%)HLAR isolates were selected.The HLAR rates for year 2006,2007,2008 and 2009 were 52.63%,65.22%,51.11%and 70.83%,respectively.Five modifying enzyme genes(aac(3)-Ⅰ,detection rate of 65.69%;aac(60)-Ⅰb,detection rate of 45.10%;aph(30)-Ⅰ,detection rate of 47.06%;aph(30)-Ⅱb,detection rate of 0.98%;ant(3″)-Ⅰ,detection rate of 95.10%)and one methylase gene(armA,detection rate of 98.04%)were detected in the 102 A.baumannii with aac(3)-Ⅰ+aac(60)-Ⅰ+þant(3″)-Ⅰ+armA(detection rate of 25.49%),aac(3)-Ⅰ+aph(30)-Ⅰ+ant(3″)-Ⅰ+armA(detection rate of 21.57%)and ant(3″)-Ⅰ+armA(detection rate of 12.75%)being the most prevalent gene profiles.The values of chi-square tests showed correlation of armA,ant(3″)-Ⅰ,aac(3)-Ⅰ,aph(30)-Ⅰand aac(60)-Ⅰb with HLAR.armA had significant correlation(contingency coefficient 0.685)and good contingency with HLAR(kappa 0.940).The high rates of HLAR may cause a serious problem for combination therapy of aminoglycoside with β-lactams against A.baumannii infections.As armA was reported to be able to cause high level aminoglycoside resistance to most of the clinical important aminoglycosides(gentamicin,amikacin,tobramycin,etc),the function of aminoglycoside modifying enzyme gene(s)in A.baumannii carrying armA deserves further investigation. | Lu Nie Yuemeng Lv Min Yuan Xinxin Hu Tongying Nie Xinyi Yang Guoqing Li Jing Pang Jingpu Zhang Congran Li Xiukun Wang Xuefu You | 2014 | Acta Pharmaceutica Sinica B2014，4，4： | 3 |
| 6 | Disulfiram thermosensitive in-situ gel based on solid dispersion for cataract显示文摘To improve the corneal permeability and water-solubility of disulfiram(DSF), which is an ocular drug for cataract, P188 was selected as a matrix to prepare solid dispersion of DSF(DSF SD) by hot melt method. The DSF SD was characterized by DSC, XRD, and IR, and the results suggested that DSF was amorphous in DSF SD. The DSF SD was added to borate buffer solution(BBS) contained 20% poloxamer P407 and 1.2% poloxamer P188 to form in-situ gel. In vitro and in vivo experiments revealed that DSF SD combined with in-situ gel(DSF SD/in-situ gel) increased the residence time and the amount of DSF penetrated through the corneal. The pharmacodynamics studies exhibited DSF SD/in-situ gel delayed the development of selenium-induced cataract at some content. These results investigated that DSF SD/in-situ gel as a drug delivery system can improve DSF ocular permeability. | Chunjuan Zhang Tonghua Xu Donglei Zhang Wei He Siling Wang Tongying Jiang | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，6： | 3 |
| 7 | Alginate encapsulated mesoporous silica nanospheres as a sustained drug delivery system for the poorly water-soluble drug indomethacin显示文摘We applied a combination of inorganic mesoporous silica material,frequently used as drug carriers,and a natural organic polymer alginate(ALG),to establish a sustained drug delivery system for the poorly water-soluble drug Indomethacin(IND).Mesoporous silica nanospheres(MSNs)were synthesized using an organic template method and then functionalized with aminopropyl groups through postsynthesis.After drug loading into the pores of aninopropyl functionalized MSNs(AP-MSNs),IND loaded AP-MSNs(IND-AP-MSNs)were encapsulated by ALG through the ionic interaction.The effects of surface chemical groups and ALG layer on IND release were systematically studied using scanning electron microscopy(SEM),transmission electron microscopy(TEM),nitrogen adsorption,zetapotential analysis and TGA analysis.The surface structure and surface charge changes of the ALG encapsulated AP-MSNs(ALG-AP-MSNs)were also investigated.The results showed that sustained release of IND from the designed drug delivery system was mainly due to the blockage effect from the coated ALG.We believe that this combination will help designing oral sustained drug delivery systems for poorly water-soluble drugs. | Liang Hu Changshan Sun Aihua Song Di Chang Xin Zheng Yikun Gao Tongying Jiang Siling Wang | 2014 | Asian Journal of Pharmaceutical Sciences2014，9，4： | 2 |
| 8 | Establishment of cell lines with porcine spermatogonial stem cell properties显示文摘Background:Spermatogonial stem cells(SSCs)are capable of both self-renewal and differentiation to mature functional spermatozoa,being the only adult stem cells in the males that can transmit genetic information to the next generation.Porcine SSCs hold great value in transgenic pig production and in establishment of porcine models for regenerative medicine.However,studies and applications of porcine SSCs have been greatly hampered by the low number of SSCs in the testis as well as the lack of an ideal stable long-term culture system to propagate porcine SSCs perpetually.Results:In the present study,by lentiviral transduction of plasmids expressing the simian virus 40(SV40)large T antigen into porcine primary SSCs,we developed two immortalized cell lines with porcine SSC attributes.The established cell lines,with the expression of porcine SSC and germ cell markers UCHL1,PLZF,THY1,VASA and DAZL,could respond to retinoic acid(RA),and could colonize the recipient mouse testis without tumor formation after transplantation.The cell lines displayed infinite proliferation potential,and have now been cultured for more than 7 months and passaged for over 35 times without morphological abnormalities.Conclusions:We have for the first time established porcine SSC lines that could provide abundant cell sources for mechanistic studies on porcine SSC self-renewal and differentiation,thereby facilitating development of an optimal long-term culture system for porcine primary SSCs and their application to animal husbandry and medicine. | Yi Zheng Tongying Feng Pengfei Zhang Peipei Lei Fuyuan Li Wenxian Zeng | 2020 | Journal of Animal Science and Biotechnology2020，11，3： | 2 |
| 9 | Polymyxin resistance caused by large-scale genomic inversion due to IS26 intramolecular translocation in Klebsiella pneumoniae显示文摘Polymyxin B and polymyxin E(colistin)are presently considered the last line of defense against human infections caused by multidrug-resistant Gram-negative organisms such as carbapenemase-producer Enterobacterales,Acinetobacter baumannii,and Klebsiella pneumoniae.Yet resistance to this last-line drugs is a major public health threat and is rapidly increasing.Polymyxin S2(S2)is a polymyxin B analogue previously synthesized in our institute with obviously high antibacterial activity and lower toxicity than polymyxin B and colistin.To predict the possible resistant mechanism of S2for wide clinical application,we experimentally induced bacterial resistant mutants and studied the preliminary resistance mechanisms.Mut-S,a resistant mutant of K.pneumoniae ATCC BAA-2146(Kpn2146)induced by S2,was analyzed by whole genome sequencing,transcriptomics,mass spectrometry and complementation experiment.Surprisingly,large-scale genomic inversion(LSGI)of approximately 1.1 Mbp in the chromosome caused by IS26mediated intramolecular transposition was found in Mut-S,which led to mgrB truncation,lipid A modification and hence S2resistance.The resistance can be complemented by plasmid carrying intact mgrB.The same mechanism was also found in polymyxin B and colistin induced drug-resistant mutants of Kpn2146(Mut-B and Mut-E,respectively).This is the first report of polymyxin resistance caused by IS26 intramolecular transposition mediated mgrB truncation in chromosome in K.pneumoniae.The findings broaden our scope of knowledge for polymyxin resistance and enriched our understanding of how bacteria can manage to survive in the presence of antibiotics. | Haibin Li Lang Sun Han Qiao Zongti Sun Penghe Wang Chunyang Xie Xinxin Hu Tongying Nie Xinyi Yang Guoqing Li Youwen Zhang Xiukun Wang Zhuorong Li Jiandong Jiang Congran Li Xuefu You | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 1 |
| 10 | 3D cubic mesoporous silica microsphere as a carrier for poorly soluble drug carvedilol显示文摘 | Yanchen Hu Zhuangzhi Zhi Qinfu Zhao Chao Wu Peng Zhao Haitao Jiang Tongying Jiang Siling Wang | 2011 | Microporous and Mesoporous Materials2011，，1： | 1 |
| 11 | Fabrication of mesoporous hydroxycarbonate apatite for oral delivery of poorly water-soluble drug carvedilol显示文摘 | Qinfu Zhao Tianyi Wang Jing Wang Li Zheng Tongying Jiang Gang Cheng Siling Wang | 2011 | Journal of Non-Crystalline Solids2011，，2： | 1 |
| 12 | Spherical mesoporous silica nanoparticles for loading and release of the poorly water-soluble drug telmisartan显示文摘 | Yanzhuo Zhang Zhuangzhi Zhi Tongying Jiang Jinghai Zhang Zhanyou Wang Siling Wang | 2010 | Journal of Controlled Release2010，，3： | 1 |
| 13 | Development of biodegradable porous starch foam for improving oral delivery of poorly water soluble drugs显示文摘 | Chao Wu Zhongyan Wang Zhuangzhi Zhi Tongying Jiang Jinghai Zhang Siling Wang | 2010 | International Journal of Pharmaceutics2010，，1： | 1 |
| 14 | Preparationtechnologyandrecentadvancesofliposomes显示文摘 | LIUXQ WANGJY TONGY etal | 2011 | 中国药学杂志2011，46，14： | 1 |
| 15 | AssociationbetweenTCF7L2genepolymorphismsandsusceptibilitytotype2diabetesmellitus:alargeHumanGenomeEpidemiology(HuGE)reviewandmeta-anal-ysis显示文摘 | TongY LinY ZhangY etal | 2009 | BMCMedGenet2009，10，： | 1 |
| 16 | Removal of sulfate and heavy metals by sulfate reducing bacteria in short-term bench scale upflow anaerobic packer bed reactor runs显示文摘 | Tongy J David L P | | 0，，： | 1 |
| 17 | High performance servo drive system of salient pole permanent magnet synchronous motor显示文摘 | MorimotoS HatanakaK TongY | 1991 | Conference record of the 1991 IEEE Industry Applications Society Annual Meeting1991，91，： | 1 |
| 18 | Switching from repression to activation: microRNAs can up-regulate translation 显示文摘 | Vasudevan S TongY Steitz JA | 2007 | Science2007，318，58： | 1 |
| 19 | A parametric formulation of the generalized spectral subtraction method 显示文摘 | SIM B L TONGY C TAN C T | 1998 | IEEE Transactions on Speech and Audio Processing1998，6，4： | 1 |
| 20 | Susceptibili tytesting of Singapore strains of Mycoplasma hominis totetracycline, gatifloxacin, moxifloxacin, ciprofloxacin, clindanycin,andazithromycin by the E -test method显示文摘 | Cecilia CL Ngan Tongying Lim Chin - Mun Choo | 2004 | Diag Microbiol Infect Dis2004，48，： | 1 |
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